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P32300

- OPRD_MOUSE

UniProt

P32300 - OPRD_MOUSE

Protein

Delta-type opioid receptor

Gene

Oprd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.3 Publications

    GO - Molecular functioni

    1. enkephalin receptor activity Source: Ensembl
    2. opioid receptor activity Source: MGI

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: MGI
    2. adult locomotory behavior Source: MGI
    3. cellular response to growth factor stimulus Source: Ensembl
    4. neuropeptide signaling pathway Source: MGI
    5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    6. protein import into nucleus, translocation Source: Ensembl
    7. regulation of calcium ion transport Source: Ensembl
    8. regulation of sensory perception of pain Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-type opioid receptor
    Short name:
    D-OR-1
    Short name:
    DOR-1
    Alternative name(s):
    K56
    MSL-2
    Gene namesi
    Name:Oprd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:97438. Oprd1.

    Subcellular locationi

    Cell membrane 4 Publications; Multi-pass membrane protein 4 Publications

    GO - Cellular componenti

    1. axon terminus Source: Ensembl
    2. cytoplasm Source: Ensembl
    3. dendrite membrane Source: Ensembl
    4. integral component of membrane Source: MGI
    5. intrinsic component of plasma membrane Source: Ensembl
    6. membrane Source: MGI
    7. membrane raft Source: Ensembl
    8. postsynaptic membrane Source: Ensembl
    9. vesicle Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Delta-type opioid receptorPRO_0000069963Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi18 – 181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi121 ↔ 1981 PublicationPROSITE-ProRule annotation
    Lipidationi333 – 3331S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP32300.

    PTM databases

    PhosphoSiteiP32300.

    Expressioni

    Tissue specificityi

    Brain, with high concentrations in the basal ganglia and limbic regions.2 Publications

    Gene expression databases

    BgeeiP32300.
    CleanExiMM_OPRD1.
    GenevestigatoriP32300.

    Interactioni

    Subunit structurei

    May form homooligomers. Interacts with GPRASP1 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-46417N.
    IntActiP32300. 7 interactions.

    Structurei

    Secondary structure

    1
    372
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi42 – 7635
    Helixi83 – 10018
    Helixi102 – 11110
    Helixi118 – 15134
    Helixi153 – 1597
    Helixi162 – 18625
    Beta strandi187 – 1926
    Beta strandi195 – 2006
    Beta strandi203 – 2053
    Helixi206 – 22015
    Helixi223 – 23816
    Helixi258 – 28629
    Helixi294 – 32128
    Helixi323 – 3264

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EJ4X-ray3.40A36-342[»]
    ProteinModelPortaliP32300.
    SMRiP32300. Positions 36-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4747Extracellular1 PublicationAdd
    BLAST
    Topological domaini76 – 8510Cytoplasmic1 Publication
    Topological domaini111 – 12212Extracellular1 PublicationAdd
    BLAST
    Topological domaini145 – 16319Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini187 – 20620Extracellular1 PublicationAdd
    BLAST
    Topological domaini239 – 26123Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini285 – 29915Extracellular1 PublicationAdd
    BLAST
    Topological domaini322 – 37251Cytoplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 7528Helical; Name=1Add
    BLAST
    Transmembranei86 – 11025Helical; Name=2Add
    BLAST
    Transmembranei123 – 14422Helical; Name=3Add
    BLAST
    Transmembranei164 – 18623Helical; Name=4Add
    BLAST
    Transmembranei207 – 23832Helical; Name=5Add
    BLAST
    Transmembranei262 – 28423Helical; Name=6Add
    BLAST
    Transmembranei300 – 32122Helical; Name=7Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG241795.
    GeneTreeiENSGT00630000089574.
    HOGENOMiHOG000230486.
    HOVERGENiHBG106919.
    InParanoidiP32300.
    KOiK04213.
    OMAiLCRKPCG.
    OrthoDBiEOG7BKCVQ.
    PhylomeDBiP32300.
    TreeFamiTF315737.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000321. Delta_opi_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001418. Opioid_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00525. DELTAOPIOIDR.
    PR00237. GPCRRHODOPSN.
    PR00384. OPIOIDR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32300-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELVPSARAE LQSSPLVNLS DAFPSAFPSA GANASGSPGA RSASSLALAI    50
    AITALYSAVC AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS 100
    TLPFQSAKYL METWPFGELL CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV 150
    CHPVKALDFR TPAKAKLINI CIWVLASGVG VPIMVMAVTQ PRDGAVVCML 200
    QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL RSVRLLSGSK 250
    EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL 300
    HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRTPCGRQ EPGSLRRPRQ 350
    ATTRERVTAC TPSDGPGGGA AA 372
    Length:372
    Mass (Da):40,561
    Last modified:October 1, 1993 - v1
    Checksum:iBC3B3BBD2E52D3F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06322 mRNA. Translation: AAA37522.1.
    L07271 mRNA. No translation available.
    L11064 mRNA. Translation: AAA37520.1.
    S65335 mRNA. Translation: AAA16009.1.
    S66181 mRNA. Translation: AAB28546.1.
    CCDSiCCDS18718.1.
    PIRiB48227.
    RefSeqiNP_038650.3. NM_013622.3.
    UniGeneiMm.5243.

    Genome annotation databases

    EnsembliENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511.
    GeneIDi18386.
    KEGGimmu:18386.
    UCSCiuc008vap.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06322 mRNA. Translation: AAA37522.1 .
    L07271 mRNA. No translation available.
    L11064 mRNA. Translation: AAA37520.1 .
    S65335 mRNA. Translation: AAA16009.1 .
    S66181 mRNA. Translation: AAB28546.1 .
    CCDSi CCDS18718.1.
    PIRi B48227.
    RefSeqi NP_038650.3. NM_013622.3.
    UniGenei Mm.5243.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EJ4 X-ray 3.40 A 36-342 [» ]
    ProteinModelPortali P32300.
    SMRi P32300. Positions 36-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46417N.
    IntActi P32300. 7 interactions.

    Chemistry

    BindingDBi P32300.
    ChEMBLi CHEMBL2221343.
    GuidetoPHARMACOLOGYi 317.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P32300.

    Proteomic databases

    PRIDEi P32300.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000056336 ; ENSMUSP00000050077 ; ENSMUSG00000050511 .
    GeneIDi 18386.
    KEGGi mmu:18386.
    UCSCi uc008vap.1. mouse.

    Organism-specific databases

    CTDi 4985.
    MGIi MGI:97438. Oprd1.

    Phylogenomic databases

    eggNOGi NOG241795.
    GeneTreei ENSGT00630000089574.
    HOGENOMi HOG000230486.
    HOVERGENi HBG106919.
    InParanoidi P32300.
    KOi K04213.
    OMAi LCRKPCG.
    OrthoDBi EOG7BKCVQ.
    PhylomeDBi P32300.
    TreeFami TF315737.

    Miscellaneous databases

    NextBioi 293976.
    PROi P32300.
    SOURCEi Search...

    Gene expression databases

    Bgeei P32300.
    CleanExi MM_OPRD1.
    Genevestigatori P32300.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000321. Delta_opi_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001418. Opioid_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00525. DELTAOPIOIDR.
    PR00237. GPCRRHODOPSN.
    PR00384. OPIOIDR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization."
      Kieffer B.L., Befort K., Gaveriaux-Ruff C., Hirth C.G.
      Proc. Natl. Acad. Sci. U.S.A. 89:12048-12052(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Cloning of a delta opioid receptor by functional expression."
      Evans C.J., Keith D.E. Jr., Morrison H., Magendzo K., Edwards R.H.
      Science 258:1952-1955(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    3. "Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
      Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
      Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    4. "Characterization and mapping of a delta opioid receptor clone from NG108-15 cells."
      Keith D.E. Jr., Anton B., Evans C.J.
      Proc. West. Pharmacol. Soc. 36:299-306(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Regional expression and chromosomal localization of the delta opiate receptor gene."
      Bzdega T., Chin H., Kim K., Jung H.H., Kozak C.A., Klee W.A.
      Proc. Natl. Acad. Sci. U.S.A. 90:9305-9309(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-372.
    6. "Retention of supraspinal delta-like analgesia and loss of morphine tolerance in delta opioid receptor knockout mice."
      Zhu Y., King M.A., Schuller A.G., Nitsche J.F., Reidl M., Elde R.P., Unterwald E., Pasternak G.W., Pintar J.E.
      Neuron 24:243-252(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "A 3D model of the delta opioid receptor and ligand-receptor complexes."
      Alkorta I., Loew G.H.
      Protein Eng. 9:573-583(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.
    8. "Structure of the delta-opioid receptor bound to naltrindole."
      Granier S., Manglik A., Kruse A.C., Kobilka T.S., Thian F.S., Weis W.I., Kobilka B.K.
      Nature 485:400-404(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 36-342 IN COMPLEX WITH NALTRINDOLE, SUBCELLULAR LOCATION, DISULFIDE BOND, TOPOLOGY.

    Entry informationi

    Entry nameiOPRD_MOUSE
    AccessioniPrimary (citable) accession number: P32300
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3