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P32300 (OPRD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-type opioid receptor

Short name=D-OR-1
Short name=DOR-1
Alternative name(s):
K56
MSL-2
Gene names
Name:Oprd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Ref.1 Ref.2 Ref.6

Subunit structure

May form homooligomers. Interacts with GPRASP1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1 Ref.2 Ref.6 Ref.8.

Tissue specificity

Brain, with high concentrations in the basal ganglia and limbic regions. Ref.2 Ref.6

Disruption phenotype

Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine. Ref.6

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 8738226. Source: MGI

adult locomotory behavior

Inferred from mutant phenotype PubMed 10835636. Source: MGI

cellular response to growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

neuropeptide signaling pathway

Inferred from direct assay Ref.3. Source: MGI

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

protein import into nucleus, translocation

Inferred from electronic annotation. Source: Ensembl

regulation of calcium ion transport

Inferred from electronic annotation. Source: Ensembl

regulation of sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentaxon terminus

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

dendrite membrane

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from direct assay Ref.3. Source: MGI

intrinsic component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from direct assay PubMed 8738226. Source: MGI

membrane raft

Inferred from electronic annotation. Source: Ensembl

postsynaptic membrane

Inferred from electronic annotation. Source: Ensembl

vesicle

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionenkephalin receptor activity

Inferred from electronic annotation. Source: Ensembl

opioid receptor activity

Inferred from direct assay Ref.3PubMed 8738226. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Delta-type opioid receptor
PRO_0000069963

Regions

Topological domain1 – 4747Extracellular Ref.8
Transmembrane48 – 7528Helical; Name=1
Topological domain76 – 8510Cytoplasmic Ref.8
Transmembrane86 – 11025Helical; Name=2
Topological domain111 – 12212Extracellular Ref.8
Transmembrane123 – 14422Helical; Name=3
Topological domain145 – 16319Cytoplasmic Ref.8
Transmembrane164 – 18623Helical; Name=4
Topological domain187 – 20620Extracellular Ref.8
Transmembrane207 – 23832Helical; Name=5
Topological domain239 – 26123Cytoplasmic Ref.8
Transmembrane262 – 28423Helical; Name=6
Topological domain285 – 29915Extracellular Ref.8
Transmembrane300 – 32122Helical; Name=7
Topological domain322 – 37251Cytoplasmic Ref.8

Amino acid modifications

Lipidation3331S-palmitoyl cysteine Potential
Glycosylation181N-linked (GlcNAc...) Potential
Glycosylation331N-linked (GlcNAc...) Potential
Disulfide bond121 ↔ 198 Ref.8

Secondary structure

........................... 372
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32300 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: BC3B3BBD2E52D3F9

FASTA37240,561
        10         20         30         40         50         60 
MELVPSARAE LQSSPLVNLS DAFPSAFPSA GANASGSPGA RSASSLALAI AITALYSAVC 

        70         80         90        100        110        120 
AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS TLPFQSAKYL METWPFGELL 

       130        140        150        160        170        180 
CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPAKAKLINI CIWVLASGVG 

       190        200        210        220        230        240 
VPIMVMAVTQ PRDGAVVCML QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL 

       250        260        270        280        290        300 
RSVRLLSGSK EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL 

       310        320        330        340        350        360 
HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRTPCGRQ EPGSLRRPRQ ATTRERVTAC 

       370 
TPSDGPGGGA AA 

« Hide

References

[1]"The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization."
Kieffer B.L., Befort K., Gaveriaux-Ruff C., Hirth C.G.
Proc. Natl. Acad. Sci. U.S.A. 89:12048-12052(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"Cloning of a delta opioid receptor by functional expression."
Evans C.J., Keith D.E. Jr., Morrison H., Magendzo K., Edwards R.H.
Science 258:1952-1955(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"Characterization and mapping of a delta opioid receptor clone from NG108-15 cells."
Keith D.E. Jr., Anton B., Evans C.J.
Proc. West. Pharmacol. Soc. 36:299-306(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Regional expression and chromosomal localization of the delta opiate receptor gene."
Bzdega T., Chin H., Kim K., Jung H.H., Kozak C.A., Klee W.A.
Proc. Natl. Acad. Sci. U.S.A. 90:9305-9309(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-372.
[6]"Retention of supraspinal delta-like analgesia and loss of morphine tolerance in delta opioid receptor knockout mice."
Zhu Y., King M.A., Schuller A.G., Nitsche J.F., Reidl M., Elde R.P., Unterwald E., Pasternak G.W., Pintar J.E.
Neuron 24:243-252(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"A 3D model of the delta opioid receptor and ligand-receptor complexes."
Alkorta I., Loew G.H.
Protein Eng. 9:573-583(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[8]"Structure of the delta-opioid receptor bound to naltrindole."
Granier S., Manglik A., Kruse A.C., Kobilka T.S., Thian F.S., Weis W.I., Kobilka B.K.
Nature 485:400-404(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 36-342 IN COMPLEX WITH NALTRINDOLE, SUBCELLULAR LOCATION, DISULFIDE BOND, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L06322 mRNA. Translation: AAA37522.1.
L07271 mRNA. No translation available.
L11064 mRNA. Translation: AAA37520.1.
S65335 mRNA. Translation: AAA16009.1.
S66181 mRNA. Translation: AAB28546.1.
CCDSCCDS18718.1.
PIRB48227.
RefSeqNP_038650.3. NM_013622.3.
UniGeneMm.5243.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EJ4X-ray3.40A36-342[»]
ProteinModelPortalP32300.
SMRP32300. Positions 36-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-46417N.
IntActP32300. 7 interactions.

Chemistry

BindingDBP32300.
ChEMBLCHEMBL2221343.
GuidetoPHARMACOLOGY317.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32300.

Proteomic databases

PRIDEP32300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511.
GeneID18386.
KEGGmmu:18386.
UCSCuc008vap.1. mouse.

Organism-specific databases

CTD4985.
MGIMGI:97438. Oprd1.

Phylogenomic databases

eggNOGNOG241795.
GeneTreeENSGT00630000089574.
HOGENOMHOG000230486.
HOVERGENHBG106919.
InParanoidP32300.
KOK04213.
OMALCRKPCG.
OrthoDBEOG7BKCVQ.
PhylomeDBP32300.
TreeFamTF315737.

Gene expression databases

BgeeP32300.
CleanExMM_OPRD1.
GenevestigatorP32300.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293976.
PROP32300.
SOURCESearch...

Entry information

Entry nameOPRD_MOUSE
AccessionPrimary (citable) accession number: P32300
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries