P32300 (OPRD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-type opioid receptor
Short name=D-OR-1
Short name=DOR-1

Alternative name(s):
K56
MSL-2

Gene names

Name:

Oprd1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.
Subunit structure	Interacts with GPRASP1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Brain, with high concentrations in the basal ganglia and limbic regions.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 8738226. Source: MGI adult locomotory behavior Inferred from mutant phenotype PubMed 10835636. Source: MGI cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara neuropeptide signaling pathway Inferred from direct assay Ref.3. Source: MGI phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein import into nucleus, translocation Inferred from electronic annotation. Source: Compara regulation of calcium ion transport Inferred from electronic annotation. Source: Compara regulation of sensory perception of pain Inferred from electronic annotation. Source: Compara
Cellular_component	axon terminus Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara dendrite membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from direct assay Ref.3. Source: MGI membrane raft Inferred from electronic annotation. Source: Compara postsynaptic membrane Inferred from electronic annotation. Source: Compara vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	enkephalin receptor activity Inferred from electronic annotation. Source: Compara opioid receptor activity Inferred from direct assay Ref.3PubMed 8738226. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 372

372

Delta-type opioid receptor

PRO_0000069963

Regions

Topological domain

1 – 45

45

Extracellular Potential

Transmembrane

46 – 75

30

Helical; Name=1; Potential

Topological domain

76 – 84

9

Cytoplasmic Potential

Transmembrane

85 – 102

18

Helical; Name=2; Potential

Topological domain

103 – 124

22

Extracellular Potential

Transmembrane

125 – 144

20

Helical; Name=3; Potential

Topological domain

145 – 174

30

Cytoplasmic Potential

Transmembrane

175 – 190

16

Helical; Name=4; Potential

Topological domain

191 – 215

25

Extracellular Potential

Transmembrane

216 – 238

23

Helical; Name=5; Potential

Topological domain

239 – 261

23

Cytoplasmic Potential

Transmembrane

262 – 284

23

Helical; Name=6; Potential

Topological domain

285 – 293

9

Extracellular Potential

Transmembrane

294 – 310

17

Helical; Name=7; Potential

Topological domain

311 – 372

62

Cytoplasmic Potential

Amino acid modifications

Lipidation

333

1

S-palmitoyl cysteine Potential

Glycosylation

18

1

N-linked (GlcNAc...) Potential

Glycosylation

33

1

N-linked (GlcNAc...) Potential

Disulfide bond

121 ↔ 198

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

372

Helix Strand Turn

Details...

Helix

42 – 76

35

Helix

83 – 100

18

Helix

102 – 111

10

Helix

118 – 151

34

Helix

153 – 159

7

Helix

162 – 186

25

Beta strand

187 – 192

6

Beta strand

195 – 200

6

Beta strand

203 – 205

3

Helix

206 – 220

15

Helix

223 – 238

16

Helix

258 – 286

29

Helix

294 – 321

28

Helix

323 – 326

4

Sequences

	Sequence		Length	Mass (Da)
	P32300 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: BC3B3BBD2E52D3F9	FASTA	372	40,561
	10 20 30 40 50 60 MELVPSARAE LQSSPLVNLS DAFPSAFPSA GANASGSPGA RSASSLALAI AITALYSAVC 70 80 90 100 110 120 AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS TLPFQSAKYL METWPFGELL 130 140 150 160 170 180 CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPAKAKLINI CIWVLASGVG 190 200 210 220 230 240 VPIMVMAVTQ PRDGAVVCML QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL 250 260 270 280 290 300 RSVRLLSGSK EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL 310 320 330 340 350 360 HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRTPCGRQ EPGSLRRPRQ ATTRERVTAC 370 TPSDGPGGGA AA

References

[1]	"The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization." Kieffer B.L., Befort K., Gaveriaux-Ruff C., Hirth C.G. Proc. Natl. Acad. Sci. U.S.A. 89:12048-12052(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of a delta opioid receptor by functional expression." Evans C.J., Keith D.E. Jr., Morrison H., Magendzo K., Edwards R.H. Science 258:1952-1955(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and functional comparison of kappa and delta opioid receptors from mouse brain." Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I. Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"Characterization and mapping of a delta opioid receptor clone from NG108-15 cells." Keith D.E. Jr., Anton B., Evans C.J. Proc. West. Pharmacol. Soc. 36:299-306(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Regional expression and chromosomal localization of the delta opiate receptor gene." Bzdega T., Chin H., Kim K., Jung H.H., Kozak C.A., Klee W.A. Proc. Natl. Acad. Sci. U.S.A. 90:9305-9309(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-372.
[6]	"A 3D model of the delta opioid receptor and ligand-receptor complexes." Alkorta I., Loew G.H. Protein Eng. 9:573-583(1996) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L06322 mRNA. Translation: AAA37522.1.
L07271 mRNA. No translation available.
L11064 mRNA. Translation: AAA37520.1.
S65335 mRNA. Translation: AAA16009.1.
S66181 mRNA. Translation: AAB28546.1.

IPI

IPI00137544.

PIR

B48227.

RefSeq

NP_038650.3. NM_013622.3.

UniGene

Mm.5243.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4EJ4	X-ray	3.40	A	36-342	[»]

ProteinModelPortal

P32300.

SMR

P32300. Positions 41-328.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46417N.

IntAct

P32300. 1 interaction.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P32300.

Proteomic databases

PRIDE

P32300.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511.

GeneID

18386.

KEGG

mmu:18386.

Organism-specific databases

CTD

4985.

MGI

MGI:97438. Oprd1.

Phylogenomic databases

eggNOG

NOG241795.

GeneTree

ENSGT00630000089574.

HOGENOM

HOG000230486.

HOVERGEN

HBG106919.

InParanoid

P32300.

KO

K04213.

OMA

LCRKPCG.

OrthoDB

EOG4XWFZ5.

Gene expression databases

ArrayExpress

P32300.

Bgee

P32300.

CleanEx

MM_OPRD1.

Genevestigator

P32300.

GermOnline

ENSMUSG00000050511. Mus musculus.

Family and domain databases

InterPro

IPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P32300.

ChEMBL

CHEMBL3222.

NextBio

293976.

SOURCE

Search...

Entry information

Entry name

OPRD_MOUSE

Accession

Primary (citable) accession number: P32300

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program