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P32300

- OPRD_MOUSE

UniProt

P32300 - OPRD_MOUSE

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Protein

Delta-type opioid receptor

Gene

Oprd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.3 Publications

GO - Molecular functioni

  1. enkephalin receptor activity Source: Ensembl
  2. opioid receptor activity Source: MGI

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: MGI
  2. adult locomotory behavior Source: MGI
  3. cellular response to growth factor stimulus Source: Ensembl
  4. neuropeptide signaling pathway Source: MGI
  5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  6. protein import into nucleus, translocation Source: Ensembl
  7. regulation of calcium ion transport Source: Ensembl
  8. regulation of sensory perception of pain Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-type opioid receptor
Short name:
D-OR-1
Short name:
DOR-1
Alternative name(s):
K56
MSL-2
Gene namesi
Name:Oprd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:97438. Oprd1.

Subcellular locationi

Cell membrane 4 Publications; Multi-pass membrane protein 4 Publications

GO - Cellular componenti

  1. axon terminus Source: Ensembl
  2. cytoplasm Source: Ensembl
  3. dendrite membrane Source: Ensembl
  4. integral component of membrane Source: MGI
  5. intrinsic component of plasma membrane Source: Ensembl
  6. membrane Source: MGI
  7. membrane raft Source: Ensembl
  8. postsynaptic membrane Source: Ensembl
  9. vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Delta-type opioid receptorPRO_0000069963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi18 – 181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi121 ↔ 1981 PublicationPROSITE-ProRule annotation
Lipidationi333 – 3331S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP32300.

PTM databases

PhosphoSiteiP32300.

Expressioni

Tissue specificityi

Brain, with high concentrations in the basal ganglia and limbic regions.2 Publications

Gene expression databases

BgeeiP32300.
CleanExiMM_OPRD1.
ExpressionAtlasiP32300. baseline and differential.
GenevestigatoriP32300.

Interactioni

Subunit structurei

May form homooligomers. Interacts with GPRASP1 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-46417N.
IntActiP32300. 7 interactions.

Structurei

Secondary structure

1
372
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi42 – 7635
Helixi83 – 10018
Helixi102 – 11110
Helixi118 – 15134
Helixi153 – 1597
Helixi162 – 18625
Beta strandi187 – 1926
Beta strandi195 – 2006
Beta strandi203 – 2053
Helixi206 – 22015
Helixi223 – 23816
Helixi258 – 28629
Helixi294 – 32128
Helixi323 – 3264

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EJ4X-ray3.40A36-342[»]
ProteinModelPortaliP32300.
SMRiP32300. Positions 36-338.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747Extracellular1 PublicationAdd
BLAST
Topological domaini76 – 8510Cytoplasmic1 Publication
Topological domaini111 – 12212Extracellular1 PublicationAdd
BLAST
Topological domaini145 – 16319Cytoplasmic1 PublicationAdd
BLAST
Topological domaini187 – 20620Extracellular1 PublicationAdd
BLAST
Topological domaini239 – 26123Cytoplasmic1 PublicationAdd
BLAST
Topological domaini285 – 29915Extracellular1 PublicationAdd
BLAST
Topological domaini322 – 37251Cytoplasmic1 PublicationAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei48 – 7528Helical; Name=1Add
BLAST
Transmembranei86 – 11025Helical; Name=2Add
BLAST
Transmembranei123 – 14422Helical; Name=3Add
BLAST
Transmembranei164 – 18623Helical; Name=4Add
BLAST
Transmembranei207 – 23832Helical; Name=5Add
BLAST
Transmembranei262 – 28423Helical; Name=6Add
BLAST
Transmembranei300 – 32122Helical; Name=7Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG241795.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP32300.
KOiK04213.
OMAiLCRKPCG.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP32300.
TreeFamiTF315737.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32300-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELVPSARAE LQSSPLVNLS DAFPSAFPSA GANASGSPGA RSASSLALAI
60 70 80 90 100
AITALYSAVC AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS
110 120 130 140 150
TLPFQSAKYL METWPFGELL CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV
160 170 180 190 200
CHPVKALDFR TPAKAKLINI CIWVLASGVG VPIMVMAVTQ PRDGAVVCML
210 220 230 240 250
QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL RSVRLLSGSK
260 270 280 290 300
EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL
310 320 330 340 350
HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRTPCGRQ EPGSLRRPRQ
360 370
ATTRERVTAC TPSDGPGGGA AA
Length:372
Mass (Da):40,561
Last modified:October 1, 1993 - v1
Checksum:iBC3B3BBD2E52D3F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06322 mRNA. Translation: AAA37522.1.
L07271 mRNA. No translation available.
L11064 mRNA. Translation: AAA37520.1.
S65335 mRNA. Translation: AAA16009.1.
S66181 mRNA. Translation: AAB28546.1.
CCDSiCCDS18718.1.
PIRiB48227.
RefSeqiNP_038650.3. NM_013622.3.
UniGeneiMm.5243.

Genome annotation databases

EnsembliENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511.
GeneIDi18386.
KEGGimmu:18386.
UCSCiuc008vap.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06322 mRNA. Translation: AAA37522.1 .
L07271 mRNA. No translation available.
L11064 mRNA. Translation: AAA37520.1 .
S65335 mRNA. Translation: AAA16009.1 .
S66181 mRNA. Translation: AAB28546.1 .
CCDSi CCDS18718.1.
PIRi B48227.
RefSeqi NP_038650.3. NM_013622.3.
UniGenei Mm.5243.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4EJ4 X-ray 3.40 A 36-342 [» ]
ProteinModelPortali P32300.
SMRi P32300. Positions 36-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-46417N.
IntActi P32300. 7 interactions.

Chemistry

BindingDBi P32300.
ChEMBLi CHEMBL2221343.
GuidetoPHARMACOLOGYi 317.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P32300.

Proteomic databases

PRIDEi P32300.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000056336 ; ENSMUSP00000050077 ; ENSMUSG00000050511 .
GeneIDi 18386.
KEGGi mmu:18386.
UCSCi uc008vap.1. mouse.

Organism-specific databases

CTDi 4985.
MGIi MGI:97438. Oprd1.

Phylogenomic databases

eggNOGi NOG241795.
GeneTreei ENSGT00760000118797.
HOGENOMi HOG000230486.
HOVERGENi HBG106919.
InParanoidi P32300.
KOi K04213.
OMAi LCRKPCG.
OrthoDBi EOG7BKCVQ.
PhylomeDBi P32300.
TreeFami TF315737.

Miscellaneous databases

NextBioi 293976.
PROi P32300.
SOURCEi Search...

Gene expression databases

Bgeei P32300.
CleanExi MM_OPRD1.
ExpressionAtlasi P32300. baseline and differential.
Genevestigatori P32300.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization."
    Kieffer B.L., Befort K., Gaveriaux-Ruff C., Hirth C.G.
    Proc. Natl. Acad. Sci. U.S.A. 89:12048-12052(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Cloning of a delta opioid receptor by functional expression."
    Evans C.J., Keith D.E. Jr., Morrison H., Magendzo K., Edwards R.H.
    Science 258:1952-1955(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
    Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
    Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  4. "Characterization and mapping of a delta opioid receptor clone from NG108-15 cells."
    Keith D.E. Jr., Anton B., Evans C.J.
    Proc. West. Pharmacol. Soc. 36:299-306(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Regional expression and chromosomal localization of the delta opiate receptor gene."
    Bzdega T., Chin H., Kim K., Jung H.H., Kozak C.A., Klee W.A.
    Proc. Natl. Acad. Sci. U.S.A. 90:9305-9309(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-372.
  6. "Retention of supraspinal delta-like analgesia and loss of morphine tolerance in delta opioid receptor knockout mice."
    Zhu Y., King M.A., Schuller A.G., Nitsche J.F., Reidl M., Elde R.P., Unterwald E., Pasternak G.W., Pintar J.E.
    Neuron 24:243-252(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "A 3D model of the delta opioid receptor and ligand-receptor complexes."
    Alkorta I., Loew G.H.
    Protein Eng. 9:573-583(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.
  8. "Structure of the delta-opioid receptor bound to naltrindole."
    Granier S., Manglik A., Kruse A.C., Kobilka T.S., Thian F.S., Weis W.I., Kobilka B.K.
    Nature 485:400-404(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 36-342 IN COMPLEX WITH NALTRINDOLE, SUBCELLULAR LOCATION, DISULFIDE BOND, TOPOLOGY.

Entry informationi

Entry nameiOPRD_MOUSE
AccessioniPrimary (citable) accession number: P32300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3