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Protein

Delta-type opioid receptor

Gene

Oprd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-type opioid receptor
Short name:
D-OR-1
Short name:
DOR-1
Alternative name(s):
K56
MSL-2
Gene namesi
Name:Oprd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:97438. Oprd1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47Extracellular1 PublicationAdd BLAST47
Transmembranei48 – 75Helical; Name=1Add BLAST28
Topological domaini76 – 85Cytoplasmic1 Publication10
Transmembranei86 – 110Helical; Name=2Add BLAST25
Topological domaini111 – 122Extracellular1 PublicationAdd BLAST12
Transmembranei123 – 144Helical; Name=3Add BLAST22
Topological domaini145 – 163Cytoplasmic1 PublicationAdd BLAST19
Transmembranei164 – 186Helical; Name=4Add BLAST23
Topological domaini187 – 206Extracellular1 PublicationAdd BLAST20
Transmembranei207 – 238Helical; Name=5Add BLAST32
Topological domaini239 – 261Cytoplasmic1 PublicationAdd BLAST23
Transmembranei262 – 284Helical; Name=6Add BLAST23
Topological domaini285 – 299Extracellular1 PublicationAdd BLAST15
Transmembranei300 – 321Helical; Name=7Add BLAST22
Topological domaini322 – 372Cytoplasmic1 PublicationAdd BLAST51

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine.1 Publication

Chemistry databases

ChEMBLiCHEMBL3222.
GuidetoPHARMACOLOGYi317.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699631 – 372Delta-type opioid receptorAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi18N-linked (GlcNAc...)Sequence analysis1
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi121 ↔ 198PROSITE-ProRule annotation1 Publication
Lipidationi333S-palmitoyl cysteineSequence analysis1

Post-translational modificationi

Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Ubl conjugation

Proteomic databases

EPDiP32300.
PaxDbiP32300.
PRIDEiP32300.

PTM databases

iPTMnetiP32300.
PhosphoSitePlusiP32300.

Expressioni

Tissue specificityi

Brain, with high concentrations in the basal ganglia and limbic regions.2 Publications

Gene expression databases

BgeeiENSMUSG00000050511.
CleanExiMM_OPRD1.
ExpressionAtlasiP32300. baseline and differential.
GenevisibleiP32300. MM.

Interactioni

Subunit structurei

May form homooligomers. Forms a heterodimer with OPRM1 (PubMed:18836069). Interacts with GPRASP1 (By similarity). Interacts with RTP4; the interaction promotes cell surface localization of the OPRD1-OPRM1 heterodimer (PubMed:18836069).By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-46417N.
IntActiP32300. 7 interactors.
STRINGi10090.ENSMUSP00000050077.

Chemistry databases

BindingDBiP32300.

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi42 – 76Combined sources35
Helixi83 – 100Combined sources18
Helixi102 – 111Combined sources10
Helixi118 – 151Combined sources34
Helixi153 – 159Combined sources7
Helixi162 – 186Combined sources25
Beta strandi187 – 192Combined sources6
Beta strandi195 – 200Combined sources6
Beta strandi203 – 205Combined sources3
Helixi206 – 220Combined sources15
Helixi223 – 238Combined sources16
Helixi258 – 286Combined sources29
Helixi294 – 321Combined sources28
Helixi323 – 326Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EJ4X-ray3.40A36-342[»]
ProteinModelPortaliP32300.
SMRiP32300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP32300.
KOiK04213.
OMAiIVRYTKL.
OrthoDBiEOG091G0HEN.
PhylomeDBiP32300.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELVPSARAE LQSSPLVNLS DAFPSAFPSA GANASGSPGA RSASSLALAI
60 70 80 90 100
AITALYSAVC AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS
110 120 130 140 150
TLPFQSAKYL METWPFGELL CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV
160 170 180 190 200
CHPVKALDFR TPAKAKLINI CIWVLASGVG VPIMVMAVTQ PRDGAVVCML
210 220 230 240 250
QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL RSVRLLSGSK
260 270 280 290 300
EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL
310 320 330 340 350
HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRTPCGRQ EPGSLRRPRQ
360 370
ATTRERVTAC TPSDGPGGGA AA
Length:372
Mass (Da):40,561
Last modified:October 1, 1993 - v1
Checksum:iBC3B3BBD2E52D3F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06322 mRNA. Translation: AAA37522.1.
L07271 mRNA. No translation available.
L11064 mRNA. Translation: AAA37520.1.
S65335 mRNA. Translation: AAA16009.1.
S66181 mRNA. Translation: AAB28546.1.
CCDSiCCDS18718.1.
PIRiB48227.
RefSeqiNP_038650.3. NM_013622.3.
UniGeneiMm.5243.

Genome annotation databases

EnsembliENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511.
GeneIDi18386.
KEGGimmu:18386.
UCSCiuc008vap.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06322 mRNA. Translation: AAA37522.1.
L07271 mRNA. No translation available.
L11064 mRNA. Translation: AAA37520.1.
S65335 mRNA. Translation: AAA16009.1.
S66181 mRNA. Translation: AAB28546.1.
CCDSiCCDS18718.1.
PIRiB48227.
RefSeqiNP_038650.3. NM_013622.3.
UniGeneiMm.5243.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EJ4X-ray3.40A36-342[»]
ProteinModelPortaliP32300.
SMRiP32300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46417N.
IntActiP32300. 7 interactors.
STRINGi10090.ENSMUSP00000050077.

Chemistry databases

BindingDBiP32300.
ChEMBLiCHEMBL3222.
GuidetoPHARMACOLOGYi317.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP32300.
PhosphoSitePlusiP32300.

Proteomic databases

EPDiP32300.
PaxDbiP32300.
PRIDEiP32300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511.
GeneIDi18386.
KEGGimmu:18386.
UCSCiuc008vap.1. mouse.

Organism-specific databases

CTDi4985.
MGIiMGI:97438. Oprd1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP32300.
KOiK04213.
OMAiIVRYTKL.
OrthoDBiEOG091G0HEN.
PhylomeDBiP32300.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP32300.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050511.
CleanExiMM_OPRD1.
ExpressionAtlasiP32300. baseline and differential.
GenevisibleiP32300. MM.

Family and domain databases

InterProiIPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPRD_MOUSE
AccessioniPrimary (citable) accession number: P32300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.