ID   BKRB2_MOUSE             Reviewed;         392 AA.
AC   P32299; B9EHE3;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 4.
DT   27-MAR-2024, entry version 184.
DE   RecName: Full=B2 bradykinin receptor;
DE            Short=B2R;
DE            Short=BK-2 receptor;
GN   Name=Bdkrb2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT), AND FUNCTION.
RC   STRAIN=C57BL/6J;
RX   PubMed=8394991;
RA   McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.;
RT   "Cloned murine bradykinin receptor exhibits a mixed B1 and B2
RT   pharmacological selectivity.";
RL   Mol. Pharmacol. 44:346-355(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE (ISOFORM SHORT), AND FUNCTION.
RC   STRAIN=129/J;
RX   PubMed=8302267;
RA   Hess J.F.R., Borkowski J.A., Macneil T., Stonesifer G.Y., Fraher J.,
RA   Strader C.D., Ransom R.W.;
RT   "Differential pharmacology of cloned human and mouse B2 bradykinin
RT   receptors.";
RL   Mol. Pharmacol. 45:1-8(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
RC   STRAIN=A/J;
RX   PubMed=8166739; DOI=10.1006/bbrc.1994.1495;
RA   Yokoyama S., Kimura Y., Taketo M., Black J.A., Ransom B.R., Higashida H.;
RT   "B2 bradykinin receptors in NG108-15 cells: cDNA cloning and functional
RT   expression.";
RL   Biochem. Biophys. Res. Commun. 200:634-641(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7959003; DOI=10.1016/0378-1119(94)90162-7;
RA   Ma J.X., Wang D.Z., Chao L., Chao J.;
RT   "Cloning, sequence analysis and expression of the gene encoding the mouse
RT   bradykinin B2 receptor.";
RL   Gene 149:283-288(1994).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   IDENTIFICATION OF LONG FORM.
RX   PubMed=8652530; DOI=10.1021/bi9601060;
RA   Abdalla S., Godovac-Zimmermann J., Braun A., Roscher A.A.,
RA   Mueller-Esterl W., Quitterer U.;
RT   "Structure of the bradykinin B2 receptors' amino terminus.";
RL   Biochemistry 35:7514-7519(1996).
CC   -!- FUNCTION: Receptor for bradykinin. It is associated with G proteins
CC       that activate a phosphatidylinositol-calcium second messenger system.
CC       {ECO:0000269|PubMed:8302267, ECO:0000269|PubMed:8394991}.
CC   -!- SUBUNIT: Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1
CC       (By similarity). {ECO:0000250|UniProtKB:P30411}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P30411};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Long;
CC         IsoId=P32299-1; Sequence=Displayed;
CC       Name=Short;
CC         IsoId=P32299-2; Sequence=VSP_001866;
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       Bradykinin receptor subfamily. BDKRB2 sub-subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; X69676; CAA49357.1; -; Genomic_DNA.
DR   EMBL; X69682; CAA49362.1; -; mRNA.
DR   EMBL; L26047; AAA19797.1; -; Unassigned_DNA.
DR   EMBL; X78438; CAA55202.1; -; mRNA.
DR   EMBL; L27595; AAA62616.1; -; Genomic_DNA.
DR   EMBL; BC137755; AAI37756.1; -; mRNA.
DR   CCDS; CCDS49160.1; -. [P32299-1]
DR   PIR; I49519; I49519.
DR   RefSeq; NP_033877.3; NM_009747.2. [P32299-1]
DR   RefSeq; XP_006515505.1; XM_006515442.3. [P32299-1]
DR   AlphaFoldDB; P32299; -.
DR   SMR; P32299; -.
DR   STRING; 10090.ENSMUSP00000001652; -.
DR   DrugCentral; P32299; -.
DR   GuidetoPHARMACOLOGY; 42; -.
DR   GlyCosmos; P32299; 3 sites, No reported glycans.
DR   GlyGen; P32299; 3 sites.
DR   iPTMnet; P32299; -.
DR   PhosphoSitePlus; P32299; -.
DR   SwissPalm; P32299; -.
DR   PaxDb; 10090-ENSMUSP00000001652; -.
DR   ProteomicsDB; 273496; -. [P32299-1]
DR   ProteomicsDB; 273497; -. [P32299-2]
DR   Antibodypedia; 14184; 335 antibodies from 35 providers.
DR   DNASU; 12062; -.
DR   Ensembl; ENSMUST00000001652.7; ENSMUSP00000001652.6; ENSMUSG00000021070.7. [P32299-1]
DR   GeneID; 12062; -.
DR   KEGG; mmu:12062; -.
DR   UCSC; uc007oym.1; mouse. [P32299-1]
DR   AGR; MGI:102845; -.
DR   CTD; 624; -.
DR   MGI; MGI:102845; Bdkrb2.
DR   VEuPathDB; HostDB:ENSMUSG00000021070; -.
DR   eggNOG; ENOG502QTX6; Eukaryota.
DR   GeneTree; ENSGT01030000234534; -.
DR   HOGENOM; CLU_009579_8_3_1; -.
DR   InParanoid; P32299; -.
DR   OMA; LAFRTMK; -.
DR   OrthoDB; 4066805at2759; -.
DR   PhylomeDB; P32299; -.
DR   TreeFam; TF330024; -.
DR   Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR   Reactome; R-MMU-416476; G alpha (q) signalling events.
DR   Reactome; R-MMU-418594; G alpha (i) signalling events.
DR   BioGRID-ORCS; 12062; 1 hit in 78 CRISPR screens.
DR   ChiTaRS; Bdkrb2; mouse.
DR   PRO; PR:P32299; -.
DR   Proteomes; UP000000589; Chromosome 12.
DR   RNAct; P32299; Protein.
DR   Bgee; ENSMUSG00000021070; Expressed in esophagus and 40 other cell types or tissues.
DR   GO; GO:0005768; C:endosome; ISO:MGI.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0031698; F:beta-2 adrenergic receptor binding; ISO:MGI.
DR   GO; GO:0004947; F:bradykinin receptor activity; ISO:MGI.
DR   GO; GO:0002020; F:protease binding; ISO:MGI.
DR   GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR   GO; GO:0031702; F:type 1 angiotensin receptor binding; ISO:MGI.
DR   GO; GO:0002438; P:acute inflammatory response to antigenic stimulus; ISO:MGI.
DR   GO; GO:0050482; P:arachidonic acid secretion; ISO:MGI.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:1990127; P:intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator; IGI:MGI.
DR   GO; GO:0035633; P:maintenance of blood-brain barrier; ISO:MGI.
DR   GO; GO:0045776; P:negative regulation of blood pressure; ISO:MGI.
DR   GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR   GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; IMP:MGI.
DR   GO; GO:1902239; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator; IGI:MGI.
DR   GO; GO:0033137; P:negative regulation of peptidyl-serine phosphorylation; IMP:MGI.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR   GO; GO:0009651; P:response to salt stress; IGI:MGI.
DR   GO; GO:0006939; P:smooth muscle contraction; IEA:InterPro.
DR   GO; GO:0042310; P:vasoconstriction; IEA:InterPro.
DR   GO; GO:0042311; P:vasodilation; IMP:MGI.
DR   CDD; cd15381; 7tmA_BK-2; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR001504; Brdyknn_2_rcpt.
DR   InterPro; IPR000496; Brdyknn_rcpt.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR10489:SF948; B2 BRADYKININ RECEPTOR; 1.
DR   PANTHER; PTHR10489; CELL ADHESION MOLECULE; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00425; BRADYKININR.
DR   PRINTS; PR00994; BRADYKINNB2R.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P32299; MM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Disulfide bond;
KW   G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW   Phosphoprotein; Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..392
FT                   /note="B2 bradykinin receptor"
FT                   /id="PRO_0000069191"
FT   TOPO_DOM        1..61
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..85
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        86..94
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..119
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        120..132
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..154
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        155..176
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..199
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..222
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..249
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        250..268
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        269..293
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        294..312
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        313..336
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        337..392
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         157
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P25023"
FT   MOD_RES         348
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P25023"
FT   MOD_RES         367
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P25023"
FT   MOD_RES         370
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P25023"
FT   MOD_RES         374
FT                   /note="Phosphoserine; by GRK6"
FT                   /evidence="ECO:0000250|UniProtKB:P30411"
FT   MOD_RES         376
FT                   /note="Phosphoserine; by GRK6"
FT                   /evidence="ECO:0000250|UniProtKB:P30411"
FT   LIPID           352
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        29
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        40
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        208
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        131..212
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   VAR_SEQ         1..26
FT                   /note="Missing (in isoform Short)"
FT                   /evidence="ECO:0000303|PubMed:8166739,
FT                   ECO:0000303|PubMed:8394991"
FT                   /id="VSP_001866"
FT   CONFLICT        298
FT                   /note="R -> A (in Ref. 1 and 4)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   392 AA;  44389 MW;  FB1AA59BCBFFF5F9 CRC64;
     MPCSWKLLGF LSVHEPMPTA ASFGIEMFNV TTQVLGSALN GTLSKDNCPD TEWWSWLNAI
     QAPFLWVLFL LAALENLFVL SVFFLHKNSC TVAEIYLGNL AAADLILACG LPFWAITIAN
     NFDWVFGEVL CRVVNTMIYM NLYSSICFLM LVSIDRYLAL VKTMSMGRMR GVRWAKLYSL
     VIWGCTLLLS SPMLVFRTMR EYSEEGHNVT ACVIVYPSRS WEVFTNVLLN LVGFLLPLSV
     ITFCTVRILQ VLRNNEMKKF KEVQTERKAT VLVLAVLGLF VLCWVPFQIS TFLDTLLRLG
     VLSGCWDEHA VDVITQISSY VAYSNSGLNP LVYVIVGKRF RKKSREVYRV LCQKGGCMGE
     PVQMENSMGT LRTSISVERQ IHKLQDWAGK KQ
//