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P32299 (BKRB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
B2 bradykinin receptor

Short name=B2R
Short name=BK-2 receptor
Gene names
Name:Bdkrb2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit structure

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarachidonic acid secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress

Inferred from mutant phenotype PubMed 16571598. Source: MGI

negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator

Inferred from genetic interaction PubMed 16571598. Source: MGI

negative regulation of peptidyl-serine phosphorylation

Inferred from mutant phenotype PubMed 16571598. Source: MGI

response to salt stress

Inferred from genetic interaction PubMed 16571598. Source: MGI

smooth muscle contraction

Inferred from electronic annotation. Source: InterPro

vasoconstriction

Inferred from electronic annotation. Source: InterPro

vasodilation

Inferred from mutant phenotype PubMed 11282893. Source: MGI

   Cellular_componentendosome

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbradykinin receptor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P32299-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P32299-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392B2 bradykinin receptor
PRO_0000069191

Regions

Topological domain1 – 6161Extracellular Potential
Transmembrane62 – 8524Helical; Name=1; Potential
Topological domain86 – 949Cytoplasmic Potential
Transmembrane95 – 11925Helical; Name=2; Potential
Topological domain120 – 13213Extracellular Potential
Transmembrane133 – 15422Helical; Name=3; Potential
Topological domain155 – 17622Cytoplasmic Potential
Transmembrane177 – 19923Helical; Name=4; Potential
Topological domain200 – 22223Extracellular Potential
Transmembrane223 – 24927Helical; Name=5; Potential
Topological domain250 – 26819Cytoplasmic Potential
Transmembrane269 – 29325Helical; Name=6; Potential
Topological domain294 – 31219Extracellular Potential
Transmembrane313 – 33624Helical; Name=7; Potential
Topological domain337 – 39256Cytoplasmic Potential

Amino acid modifications

Modified residue1571Phosphotyrosine By similarity
Modified residue3481Phosphotyrosine By similarity
Modified residue3671Phosphoserine By similarity
Modified residue3701Phosphothreonine By similarity
Modified residue3741Phosphoserine; by GRK6 By similarity
Modified residue3761Phosphoserine; by GRK6 By similarity
Lipidation3521S-palmitoyl cysteine Potential
Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Disulfide bond131 ↔ 212 By similarity

Natural variations

Alternative sequence1 – 2626Missing in isoform Short.
VSP_001866

Experimental info

Sequence conflict2981R → A Ref.1
Sequence conflict2981R → A Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 30, 2000. Version 4.
Checksum: FB1AA59BCBFFF5F9

FASTA39244,389
        10         20         30         40         50         60 
MPCSWKLLGF LSVHEPMPTA ASFGIEMFNV TTQVLGSALN GTLSKDNCPD TEWWSWLNAI 

        70         80         90        100        110        120 
QAPFLWVLFL LAALENLFVL SVFFLHKNSC TVAEIYLGNL AAADLILACG LPFWAITIAN 

       130        140        150        160        170        180 
NFDWVFGEVL CRVVNTMIYM NLYSSICFLM LVSIDRYLAL VKTMSMGRMR GVRWAKLYSL 

       190        200        210        220        230        240 
VIWGCTLLLS SPMLVFRTMR EYSEEGHNVT ACVIVYPSRS WEVFTNVLLN LVGFLLPLSV 

       250        260        270        280        290        300 
ITFCTVRILQ VLRNNEMKKF KEVQTERKAT VLVLAVLGLF VLCWVPFQIS TFLDTLLRLG 

       310        320        330        340        350        360 
VLSGCWDEHA VDVITQISSY VAYSNSGLNP LVYVIVGKRF RKKSREVYRV LCQKGGCMGE 

       370        380        390 
PVQMENSMGT LRTSISVERQ IHKLQDWAGK KQ 

« Hide

Isoform Short [UniParc].

Checksum: 9276DBA985F42379
Show »

FASTA36641,558

References

« Hide 'large scale' references
[1]"Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
Strain: C57BL/6.
[2]"Differential pharmacology of cloned human and mouse B2 bradykinin receptors."
Hess J.F.R., Borkowski J.A., Macneil T., Stonesifer G.Y., Fraher J., Strader C.D., Ransom R.W.
Mol. Pharmacol. 45:1-8(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT).
Strain: 129/J.
[3]"B2 bradykinin receptors in NG108-15 cells: cDNA cloning and functional expression."
Yokoyama S., Kimura Y., Taketo M., Black J.A., Ransom B.R., Higashida H.
Biochem. Biophys. Res. Commun. 200:634-641(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Strain: A/J.
[4]"Cloning, sequence analysis and expression of the gene encoding the mouse bradykinin B2 receptor."
Ma J.X., Wang D.Z., Chao L., Chao J.
Gene 149:283-288(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Testis.
[6]"Structure of the bradykinin B2 receptors' amino terminus."
Abdalla S., Godovac-Zimmermann J., Braun A., Roscher A.A., Mueller-Esterl W., Quitterer U.
Biochemistry 35:7514-7519(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF LONG FORM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69676 Genomic DNA. Translation: CAA49357.1.
X69682 mRNA. Translation: CAA49362.1.
L26047 Unassigned DNA. Translation: AAA19797.1.
X78438 mRNA. Translation: CAA55202.1.
L27595 Genomic DNA. Translation: AAA62616.1.
BC137755 mRNA. Translation: AAI37756.1.
CCDSCCDS49160.1. [P32299-1]
PIRI49519.
RefSeqNP_033877.3. NM_009747.2. [P32299-1]
XP_006515505.1. XM_006515442.1. [P32299-1]
UniGeneMm.390475.

3D structure databases

ProteinModelPortalP32299.
SMRP32299. Positions 57-341.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY42.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32299.

Proteomic databases

PRIDEP32299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001652; ENSMUSP00000001652; ENSMUSG00000021070. [P32299-1]
GeneID12062.
KEGGmmu:12062.
UCSCuc007oym.1. mouse. [P32299-1]

Organism-specific databases

CTD624.
MGIMGI:102845. Bdkrb2.

Phylogenomic databases

eggNOGNOG148810.
GeneTreeENSGT00720000108671.
HOGENOMHOG000010314.
HOVERGENHBG103183.
InParanoidP32299.
KOK03916.
OMAHKSSCTV.
OrthoDBEOG7HHWSV.
PhylomeDBP32299.
TreeFamTF330024.

Gene expression databases

BgeeP32299.
CleanExMM_BDKRB2.
GenevestigatorP32299.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280373.
PROP32299.
SOURCESearch...

Entry information

Entry nameBKRB2_MOUSE
AccessionPrimary (citable) accession number: P32299
Secondary accession number(s): B9EHE3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries