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P32299

- BKRB2_MOUSE

UniProt

P32299 - BKRB2_MOUSE

Protein

B2 bradykinin receptor

Gene

Bdkrb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 4 (30 May 2000)
      Previous versions | rss
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    Functioni

    Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

    GO - Molecular functioni

    1. bradykinin receptor activity Source: Ensembl

    GO - Biological processi

    1. arachidonic acid secretion Source: Ensembl
    2. negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: MGI
    3. negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator Source: MGI
    4. negative regulation of peptidyl-serine phosphorylation Source: MGI
    5. response to salt stress Source: MGI
    6. smooth muscle contraction Source: InterPro
    7. vasoconstriction Source: InterPro
    8. vasodilation Source: MGI

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    B2 bradykinin receptor
    Short name:
    B2R
    Short name:
    BK-2 receptor
    Gene namesi
    Name:Bdkrb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:102845. Bdkrb2.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392B2 bradykinin receptorPRO_0000069191Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi131 ↔ 212PROSITE-ProRule annotation
    Modified residuei157 – 1571PhosphotyrosineBy similarity
    Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
    Modified residuei348 – 3481PhosphotyrosineBy similarity
    Lipidationi352 – 3521S-palmitoyl cysteineSequence Analysis
    Modified residuei367 – 3671PhosphoserineBy similarity
    Modified residuei370 – 3701PhosphothreonineBy similarity
    Modified residuei374 – 3741Phosphoserine; by GRK6By similarity
    Modified residuei376 – 3761Phosphoserine; by GRK6By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP32299.

    PTM databases

    PhosphoSiteiP32299.

    Expressioni

    Gene expression databases

    BgeeiP32299.
    CleanExiMM_BDKRB2.
    GenevestigatoriP32299.

    Interactioni

    Subunit structurei

    Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP32299.
    SMRiP32299. Positions 57-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6161ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini86 – 949CytoplasmicSequence Analysis
    Topological domaini120 – 13213ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini155 – 17622CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini200 – 22223ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini250 – 26819CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini294 – 31219ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini337 – 39256CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei62 – 8524Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei95 – 11925Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei133 – 15422Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei177 – 19923Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei223 – 24927Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei269 – 29325Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei313 – 33624Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148810.
    GeneTreeiENSGT00720000108671.
    HOGENOMiHOG000010314.
    HOVERGENiHBG103183.
    InParanoidiP32299.
    KOiK03916.
    OMAiHKSSCTV.
    OrthoDBiEOG7HHWSV.
    PhylomeDBiP32299.
    TreeFamiTF330024.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001504. Brdyknn_2_rcpt.
    IPR000496. Brdyknn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00425. BRADYKININR.
    PR00994. BRADYKINNB2R.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P32299-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPCSWKLLGF LSVHEPMPTA ASFGIEMFNV TTQVLGSALN GTLSKDNCPD    50
    TEWWSWLNAI QAPFLWVLFL LAALENLFVL SVFFLHKNSC TVAEIYLGNL 100
    AAADLILACG LPFWAITIAN NFDWVFGEVL CRVVNTMIYM NLYSSICFLM 150
    LVSIDRYLAL VKTMSMGRMR GVRWAKLYSL VIWGCTLLLS SPMLVFRTMR 200
    EYSEEGHNVT ACVIVYPSRS WEVFTNVLLN LVGFLLPLSV ITFCTVRILQ 250
    VLRNNEMKKF KEVQTERKAT VLVLAVLGLF VLCWVPFQIS TFLDTLLRLG 300
    VLSGCWDEHA VDVITQISSY VAYSNSGLNP LVYVIVGKRF RKKSREVYRV 350
    LCQKGGCMGE PVQMENSMGT LRTSISVERQ IHKLQDWAGK KQ 392
    Length:392
    Mass (Da):44,389
    Last modified:May 30, 2000 - v4
    Checksum:iFB1AA59BCBFFF5F9
    GO
    Isoform Short (identifier: P32299-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-26: Missing.

    Show »
    Length:366
    Mass (Da):41,558
    Checksum:i9276DBA985F42379
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti298 – 2981R → A(PubMed:8394991)Curated
    Sequence conflicti298 – 2981R → A(PubMed:7959003)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2626Missing in isoform Short. 2 PublicationsVSP_001866Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69676 Genomic DNA. Translation: CAA49357.1.
    X69682 mRNA. Translation: CAA49362.1.
    L26047 Unassigned DNA. Translation: AAA19797.1.
    X78438 mRNA. Translation: CAA55202.1.
    L27595 Genomic DNA. Translation: AAA62616.1.
    BC137755 mRNA. Translation: AAI37756.1.
    CCDSiCCDS49160.1. [P32299-1]
    PIRiI49519.
    RefSeqiNP_033877.3. NM_009747.2. [P32299-1]
    XP_006515505.1. XM_006515442.1. [P32299-1]
    UniGeneiMm.390475.

    Genome annotation databases

    EnsembliENSMUST00000001652; ENSMUSP00000001652; ENSMUSG00000021070. [P32299-1]
    GeneIDi12062.
    KEGGimmu:12062.
    UCSCiuc007oym.1. mouse. [P32299-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69676 Genomic DNA. Translation: CAA49357.1 .
    X69682 mRNA. Translation: CAA49362.1 .
    L26047 Unassigned DNA. Translation: AAA19797.1 .
    X78438 mRNA. Translation: CAA55202.1 .
    L27595 Genomic DNA. Translation: AAA62616.1 .
    BC137755 mRNA. Translation: AAI37756.1 .
    CCDSi CCDS49160.1. [P32299-1 ]
    PIRi I49519.
    RefSeqi NP_033877.3. NM_009747.2. [P32299-1 ]
    XP_006515505.1. XM_006515442.1. [P32299-1 ]
    UniGenei Mm.390475.

    3D structure databases

    ProteinModelPortali P32299.
    SMRi P32299. Positions 57-341.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    GuidetoPHARMACOLOGYi 42.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P32299.

    Proteomic databases

    PRIDEi P32299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001652 ; ENSMUSP00000001652 ; ENSMUSG00000021070 . [P32299-1 ]
    GeneIDi 12062.
    KEGGi mmu:12062.
    UCSCi uc007oym.1. mouse. [P32299-1 ]

    Organism-specific databases

    CTDi 624.
    MGIi MGI:102845. Bdkrb2.

    Phylogenomic databases

    eggNOGi NOG148810.
    GeneTreei ENSGT00720000108671.
    HOGENOMi HOG000010314.
    HOVERGENi HBG103183.
    InParanoidi P32299.
    KOi K03916.
    OMAi HKSSCTV.
    OrthoDBi EOG7HHWSV.
    PhylomeDBi P32299.
    TreeFami TF330024.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 280373.
    PROi P32299.
    SOURCEi Search...

    Gene expression databases

    Bgeei P32299.
    CleanExi MM_BDKRB2.
    Genevestigatori P32299.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001504. Brdyknn_2_rcpt.
    IPR000496. Brdyknn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00425. BRADYKININR.
    PR00994. BRADYKINNB2R.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
      McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
      Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
      Strain: C57BL/6.
    2. "Differential pharmacology of cloned human and mouse B2 bradykinin receptors."
      Hess J.F.R., Borkowski J.A., Macneil T., Stonesifer G.Y., Fraher J., Strader C.D., Ransom R.W.
      Mol. Pharmacol. 45:1-8(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT).
      Strain: 129/J.
    3. "B2 bradykinin receptors in NG108-15 cells: cDNA cloning and functional expression."
      Yokoyama S., Kimura Y., Taketo M., Black J.A., Ransom B.R., Higashida H.
      Biochem. Biophys. Res. Commun. 200:634-641(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Strain: A/J.
    4. "Cloning, sequence analysis and expression of the gene encoding the mouse bradykinin B2 receptor."
      Ma J.X., Wang D.Z., Chao L., Chao J.
      Gene 149:283-288(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Testis.
    6. Cited for: IDENTIFICATION OF LONG FORM.

    Entry informationi

    Entry nameiBKRB2_MOUSE
    AccessioniPrimary (citable) accession number: P32299
    Secondary accession number(s): B9EHE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 127 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3