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Protein

B2 bradykinin receptor

Gene

Bdkrb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  1. bradykinin receptor activity Source: MGI
  2. protease binding Source: MGI
  3. protein heterodimerization activity Source: MGI
  4. type 1 angiotensin receptor binding Source: MGI

GO - Biological processi

  1. arachidonic acid secretion Source: MGI
  2. negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: MGI
  3. negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator Source: MGI
  4. negative regulation of peptidyl-serine phosphorylation Source: MGI
  5. response to salt stress Source: MGI
  6. smooth muscle contraction Source: InterPro
  7. vasoconstriction Source: InterPro
  8. vasodilation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.
REACT_250376. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
B2 bradykinin receptor
Short name:
B2R
Short name:
BK-2 receptor
Gene namesi
Name:Bdkrb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:102845. Bdkrb2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6161ExtracellularSequence AnalysisAdd
BLAST
Transmembranei62 – 8524Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini86 – 949CytoplasmicSequence Analysis
Transmembranei95 – 11925Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini120 – 13213ExtracellularSequence AnalysisAdd
BLAST
Transmembranei133 – 15422Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini155 – 17622CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei177 – 19923Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini200 – 22223ExtracellularSequence AnalysisAdd
BLAST
Transmembranei223 – 24927Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini250 – 26819CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei269 – 29325Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini294 – 31219ExtracellularSequence AnalysisAdd
BLAST
Transmembranei313 – 33624Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini337 – 39256CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endosome Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392B2 bradykinin receptorPRO_0000069191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi131 ↔ 212PROSITE-ProRule annotation
Modified residuei157 – 1571PhosphotyrosineBy similarity
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
Modified residuei348 – 3481PhosphotyrosineBy similarity
Lipidationi352 – 3521S-palmitoyl cysteineSequence Analysis
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei370 – 3701PhosphothreonineBy similarity
Modified residuei374 – 3741Phosphoserine; by GRK6By similarity
Modified residuei376 – 3761Phosphoserine; by GRK6By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP32299.

PTM databases

PhosphoSiteiP32299.

Expressioni

Gene expression databases

BgeeiP32299.
CleanExiMM_BDKRB2.
ExpressionAtlasiP32299. baseline and differential.
GenevestigatoriP32299.

Interactioni

Subunit structurei

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP32299.
SMRiP32299. Positions 57-341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148810.
GeneTreeiENSGT00760000119055.
HOGENOMiHOG000010314.
HOVERGENiHBG103183.
InParanoidiP32299.
KOiK03916.
OMAiHKSSCTV.
OrthoDBiEOG7HHWSV.
PhylomeDBiP32299.
TreeFamiTF330024.

Family and domain databases

InterProiIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P32299-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPCSWKLLGF LSVHEPMPTA ASFGIEMFNV TTQVLGSALN GTLSKDNCPD
60 70 80 90 100
TEWWSWLNAI QAPFLWVLFL LAALENLFVL SVFFLHKNSC TVAEIYLGNL
110 120 130 140 150
AAADLILACG LPFWAITIAN NFDWVFGEVL CRVVNTMIYM NLYSSICFLM
160 170 180 190 200
LVSIDRYLAL VKTMSMGRMR GVRWAKLYSL VIWGCTLLLS SPMLVFRTMR
210 220 230 240 250
EYSEEGHNVT ACVIVYPSRS WEVFTNVLLN LVGFLLPLSV ITFCTVRILQ
260 270 280 290 300
VLRNNEMKKF KEVQTERKAT VLVLAVLGLF VLCWVPFQIS TFLDTLLRLG
310 320 330 340 350
VLSGCWDEHA VDVITQISSY VAYSNSGLNP LVYVIVGKRF RKKSREVYRV
360 370 380 390
LCQKGGCMGE PVQMENSMGT LRTSISVERQ IHKLQDWAGK KQ
Length:392
Mass (Da):44,389
Last modified:May 30, 2000 - v4
Checksum:iFB1AA59BCBFFF5F9
GO
Isoform Short (identifier: P32299-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:366
Mass (Da):41,558
Checksum:i9276DBA985F42379
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti298 – 2981R → A(PubMed:8394991)Curated
Sequence conflicti298 – 2981R → A(PubMed:7959003)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2626Missing in isoform Short. 2 PublicationsVSP_001866Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69676 Genomic DNA. Translation: CAA49357.1.
X69682 mRNA. Translation: CAA49362.1.
L26047 Unassigned DNA. Translation: AAA19797.1.
X78438 mRNA. Translation: CAA55202.1.
L27595 Genomic DNA. Translation: AAA62616.1.
BC137755 mRNA. Translation: AAI37756.1.
CCDSiCCDS49160.1. [P32299-1]
PIRiI49519.
RefSeqiNP_033877.3. NM_009747.2. [P32299-1]
XP_006515505.1. XM_006515442.1. [P32299-1]
UniGeneiMm.390475.

Genome annotation databases

EnsembliENSMUST00000001652; ENSMUSP00000001652; ENSMUSG00000021070. [P32299-1]
GeneIDi12062.
KEGGimmu:12062.
UCSCiuc007oym.1. mouse. [P32299-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69676 Genomic DNA. Translation: CAA49357.1.
X69682 mRNA. Translation: CAA49362.1.
L26047 Unassigned DNA. Translation: AAA19797.1.
X78438 mRNA. Translation: CAA55202.1.
L27595 Genomic DNA. Translation: AAA62616.1.
BC137755 mRNA. Translation: AAI37756.1.
CCDSiCCDS49160.1. [P32299-1]
PIRiI49519.
RefSeqiNP_033877.3. NM_009747.2. [P32299-1]
XP_006515505.1. XM_006515442.1. [P32299-1]
UniGeneiMm.390475.

3D structure databases

ProteinModelPortaliP32299.
SMRiP32299. Positions 57-341.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi42.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP32299.

Proteomic databases

PRIDEiP32299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001652; ENSMUSP00000001652; ENSMUSG00000021070. [P32299-1]
GeneIDi12062.
KEGGimmu:12062.
UCSCiuc007oym.1. mouse. [P32299-1]

Organism-specific databases

CTDi624.
MGIiMGI:102845. Bdkrb2.

Phylogenomic databases

eggNOGiNOG148810.
GeneTreeiENSGT00760000119055.
HOGENOMiHOG000010314.
HOVERGENiHBG103183.
InParanoidiP32299.
KOiK03916.
OMAiHKSSCTV.
OrthoDBiEOG7HHWSV.
PhylomeDBiP32299.
TreeFamiTF330024.

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.
REACT_250376. G alpha (i) signalling events.

Miscellaneous databases

NextBioi280373.
PROiP32299.
SOURCEiSearch...

Gene expression databases

BgeeiP32299.
CleanExiMM_BDKRB2.
ExpressionAtlasiP32299. baseline and differential.
GenevestigatoriP32299.

Family and domain databases

InterProiIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
    McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
    Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
    Strain: C57BL/6.
  2. "Differential pharmacology of cloned human and mouse B2 bradykinin receptors."
    Hess J.F.R., Borkowski J.A., Macneil T., Stonesifer G.Y., Fraher J., Strader C.D., Ransom R.W.
    Mol. Pharmacol. 45:1-8(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT).
    Strain: 129/J.
  3. "B2 bradykinin receptors in NG108-15 cells: cDNA cloning and functional expression."
    Yokoyama S., Kimura Y., Taketo M., Black J.A., Ransom B.R., Higashida H.
    Biochem. Biophys. Res. Commun. 200:634-641(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Strain: A/J.
  4. "Cloning, sequence analysis and expression of the gene encoding the mouse bradykinin B2 receptor."
    Ma J.X., Wang D.Z., Chao L., Chao J.
    Gene 149:283-288(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Tissue: Testis.
  6. Cited for: IDENTIFICATION OF LONG FORM.

Entry informationi

Entry nameiBKRB2_MOUSE
AccessioniPrimary (citable) accession number: P32299
Secondary accession number(s): B9EHE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: February 4, 2015
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.