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P32296 (P5CS_VIGAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-1-pyrroline-5-carboxylate synthase

Short name=P5CS

Including the following 2 domains:

  1. Glutamate 5-kinase
    Short name=GK
    EC=2.7.2.11
    Alternative name(s):
    Gamma-glutamyl kinase
  2. Gamma-glutamyl phosphate reductase
    Short name=GPR
    EC=1.2.1.41
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
OrganismVigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Taxonomic identifier3918 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Protein attributes

Sequence length671 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulation

Feedback regulated by proline.

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.

Tissue specificity

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Sequence similarities

In the N-terminal section; belongs to the glutamate 5-kinase family.

In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 671671Delta-1-pyrroline-5-carboxylate synthase
PRO_0000109778

Regions

Nucleotide binding197 – 1982ATP By similarity
Nucleotide binding237 – 2437ATP By similarity
Region1 – 297297Glutamate 5-kinase
Region298 – 671374Gamma-glutamyl phosphate reductase

Sites

Binding site611Substrate By similarity
Binding site1581Substrate By similarity
Binding site1771Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P32296 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 30BAE7AAF7ED1DA6

FASTA67173,291
        10         20         30         40         50         60 
MESAVDPSRG FMKDVKRVII KVGTAVVTRE EGRLAVGRLG ALCEQIKQLN SLGYDIILVS 

        70         80         90        100        110        120 
SGPVGIGRQR LRFRKLINSS FADLQKPQLE LDGKACAAVG QNSLMALYDT LFTQLDVTSA 

       130        140        150        160        170        180 
QLLVTDNDFR DKDFRKQLTE TVKSLLALKV IPVFNENDAV STRKAPYEDS SGIFWDNDSL 

       190        200        210        220        230        240 
SALLALELKA DLLVLLSDVE GLYSGPPSDP HSKLIYTYNK EKHQNEITFG DKSRVGRGGM 

       250        260        270        280        290        300 
TAKVKAAVHA AEAGIPVVIT SGFAPENIIN VLQGQRIGTL FHKDAHEWAQ VKEVDAREMA 

       310        320        330        340        350        360 
VAAGNVREGS RRYLQRKGNK ILLKIADALE ANEKIIRIEN EADVTAAQEA GYEKSLVARL 

       370        380        390        400        410        420 
ALKPGKIASL ANNMRIIANM EDPIGRVLKR TELSDGLILE KTSSPLGVLL IVFESRPDAL 

       430        440        450        460        470        480 
VQIASLAIRS GNGLLLKGGK EAKRSNAILH KVIIEAIPDN VGGKLIGLVT SREEIPELLK 

       490        500        510        520        530        540 
LDDVIDLVIP RGSNKLVSQI KSSTKIPVLG HADGICHVYV DKSANVEMAK RIVLDAKVDY 

       550        560        570        580        590        600 
PAACNAMETL LIHKDLIEKG WLKEIILDLR TEGVILYGGP VASSLLNIPQ AHSFHHEYSS 

       610        620        630        640        650        660 
LACTAEIVDD VYAAIDHINL YGSAHTDSIV AEDNEVANVF LRQVDSAAVF HNASTRFSDG 

       670 
ARFETRRRGW N 

« Hide

References

[1]"A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants."
Hu C.-A.A., Delauney A.J., Verma D.P.S.
Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92276 mRNA. No translation available.
PIRA46295.

3D structure databases

ProteinModelPortalP32296.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP32296.
UniPathwayUPA00098; UER00359.
UPA00098; UER00360.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF036429. P5C_syn. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5CS_VIGAC
AccessionPrimary (citable) accession number: P32296
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways