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P32296

- P5CS_VIGAC

UniProt

P32296 - P5CS_VIGAC

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Protein
Delta-1-pyrroline-5-carboxylate synthase
Gene
N/A
Organism
Vigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Substrate By similarity
Binding sitei158 – 1581Substrate By similarity
Binding sitei177 – 1771Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 1982ATP By similarity
Nucleotide bindingi237 – 2437ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP32296.
UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
OrganismiVigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Taxonomic identifieri3918 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 671671Delta-1-pyrroline-5-carboxylate synthase
PRO_0000109778Add
BLAST

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Structurei

3D structure databases

ProteinModelPortaliP32296.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 297297Glutamate 5-kinase
Add
BLAST
Regioni298 – 671374Gamma-glutamyl phosphate reductase
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32296-1 [UniParc]FASTAAdd to Basket

« Hide

MESAVDPSRG FMKDVKRVII KVGTAVVTRE EGRLAVGRLG ALCEQIKQLN    50
SLGYDIILVS SGPVGIGRQR LRFRKLINSS FADLQKPQLE LDGKACAAVG 100
QNSLMALYDT LFTQLDVTSA QLLVTDNDFR DKDFRKQLTE TVKSLLALKV 150
IPVFNENDAV STRKAPYEDS SGIFWDNDSL SALLALELKA DLLVLLSDVE 200
GLYSGPPSDP HSKLIYTYNK EKHQNEITFG DKSRVGRGGM TAKVKAAVHA 250
AEAGIPVVIT SGFAPENIIN VLQGQRIGTL FHKDAHEWAQ VKEVDAREMA 300
VAAGNVREGS RRYLQRKGNK ILLKIADALE ANEKIIRIEN EADVTAAQEA 350
GYEKSLVARL ALKPGKIASL ANNMRIIANM EDPIGRVLKR TELSDGLILE 400
KTSSPLGVLL IVFESRPDAL VQIASLAIRS GNGLLLKGGK EAKRSNAILH 450
KVIIEAIPDN VGGKLIGLVT SREEIPELLK LDDVIDLVIP RGSNKLVSQI 500
KSSTKIPVLG HADGICHVYV DKSANVEMAK RIVLDAKVDY PAACNAMETL 550
LIHKDLIEKG WLKEIILDLR TEGVILYGGP VASSLLNIPQ AHSFHHEYSS 600
LACTAEIVDD VYAAIDHINL YGSAHTDSIV AEDNEVANVF LRQVDSAAVF 650
HNASTRFSDG ARFETRRRGW N 671
Length:671
Mass (Da):73,291
Last modified:October 1, 1993 - v1
Checksum:i30BAE7AAF7ED1DA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92276 mRNA. No translation available.
PIRiA46295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92276 mRNA. No translation available.
PIRi A46295.

3D structure databases

ProteinModelPortali P32296.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
UPA00098 ; UER00360 .
SABIO-RK P32296.

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPi MF_00412. ProA.
MF_00456. ProB.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036429. P5C_syn. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants."
    Hu C.-A.A., Delauney A.J., Verma D.P.S.
    Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiP5CS_VIGAC
AccessioniPrimary (citable) accession number: P32296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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