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P32296

- P5CS_VIGAC

UniProt

P32296 - P5CS_VIGAC

Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene
N/A
Organism
Vigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
    L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

    Enzyme regulationi

    Feedback regulated by proline.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611SubstrateBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei177 – 1771Substrate; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 1982ATPBy similarity
    Nucleotide bindingi237 – 2437ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-EC
    3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP32296.
    UniPathwayiUPA00098; UER00359.
    UPA00098; UER00360.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-1-pyrroline-5-carboxylate synthase
    Short name:
    P5CS
    Including the following 2 domains:
    Glutamate 5-kinase (EC:2.7.2.11)
    Short name:
    GK
    Alternative name(s):
    Gamma-glutamyl kinase
    Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
    Short name:
    GPR
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
    OrganismiVigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
    Taxonomic identifieri3918 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 671671Delta-1-pyrroline-5-carboxylate synthasePRO_0000109778Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

    Structurei

    3D structure databases

    ProteinModelPortaliP32296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 297297Glutamate 5-kinaseAdd
    BLAST
    Regioni298 – 671374Gamma-glutamyl phosphate reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
    In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

    Family and domain databases

    Gene3Di3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    HAMAPiMF_00412. ProA.
    MF_00456. ProB.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036429. P5C_syn. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESAVDPSRG FMKDVKRVII KVGTAVVTRE EGRLAVGRLG ALCEQIKQLN    50
    SLGYDIILVS SGPVGIGRQR LRFRKLINSS FADLQKPQLE LDGKACAAVG 100
    QNSLMALYDT LFTQLDVTSA QLLVTDNDFR DKDFRKQLTE TVKSLLALKV 150
    IPVFNENDAV STRKAPYEDS SGIFWDNDSL SALLALELKA DLLVLLSDVE 200
    GLYSGPPSDP HSKLIYTYNK EKHQNEITFG DKSRVGRGGM TAKVKAAVHA 250
    AEAGIPVVIT SGFAPENIIN VLQGQRIGTL FHKDAHEWAQ VKEVDAREMA 300
    VAAGNVREGS RRYLQRKGNK ILLKIADALE ANEKIIRIEN EADVTAAQEA 350
    GYEKSLVARL ALKPGKIASL ANNMRIIANM EDPIGRVLKR TELSDGLILE 400
    KTSSPLGVLL IVFESRPDAL VQIASLAIRS GNGLLLKGGK EAKRSNAILH 450
    KVIIEAIPDN VGGKLIGLVT SREEIPELLK LDDVIDLVIP RGSNKLVSQI 500
    KSSTKIPVLG HADGICHVYV DKSANVEMAK RIVLDAKVDY PAACNAMETL 550
    LIHKDLIEKG WLKEIILDLR TEGVILYGGP VASSLLNIPQ AHSFHHEYSS 600
    LACTAEIVDD VYAAIDHINL YGSAHTDSIV AEDNEVANVF LRQVDSAAVF 650
    HNASTRFSDG ARFETRRRGW N 671
    Length:671
    Mass (Da):73,291
    Last modified:October 1, 1993 - v1
    Checksum:i30BAE7AAF7ED1DA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92276 mRNA. No translation available.
    PIRiA46295.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92276 mRNA. No translation available.
    PIRi A46295.

    3D structure databases

    ProteinModelPortali P32296.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    UPA00098 ; UER00360 .
    SABIO-RK P32296.

    Family and domain databases

    Gene3Di 3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    HAMAPi MF_00412. ProA.
    MF_00456. ProB.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036429. P5C_syn. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants."
      Hu C.-A.A., Delauney A.J., Verma D.P.S.
      Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiP5CS_VIGAC
    AccessioniPrimary (citable) accession number: P32296
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3