Reviewed, UniProtKB/Swiss-Prot P32289 (GLNA_VIGAC)

Names and origin

Protein names	Recommended name:     Glutamine synthetase nodule isozyme       Short name=GS     EC=6.3.1.2 Alternative name(s):     Glutamate--ammonia ligase
Organism	Vigna aconitifolia (Mothbean)
Taxonomic identifier	3918 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Glutamine synthetase nodule isozyme		PRO_0000153198

Sequences

	Sequence		Length	Mass (Da)
	P32289-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: FF62D00D9BE16745	FASTA	356	39,104
	10 20 30 40 50 60 MSLLSDLINL NLSDTTEKII AEYIWIGGSG LDLRSKARTL PGPVSDPSKL PKWNYDGSST 70 80 90 100 110 120 GQAPGEDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE PIPTNKRHNA AKIFSHPDVV 130 140 150 160 170 180 AEEPWYGIEQ EYTLLQKDVN WPLGWPVGGF PGPQGPYYCG AGADKAFGRD IVDAHYKACL 190 200 210 220 230 240 YAGINISGIN GEVMPGQWEF QVGPAVGISA GDELWVARYI LERITEIAGV VLSFDPKPIK 250 260 270 280 290 300 GDWNGAGAHT NYSTKTMRND GGYEVIKSAI EKLGKRHKEH IAAYGEGNER RLTGRHETAD 310 320 330 340 350 INTFLWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIADT TILWKP

References

[1]	"A nodulin-specific glutamine synthetase from Vigna aconitifolia." Lin Z., Miao G.H., Verma D.P.S. Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94765 mRNA. Translation: AAA34239.1.
3D structure databases
SMR	P32289. Positions 4-355.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.1.2. 18771.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA_VIGAC

Accession

Primary (citable) accession number: P32289

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)