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Protein

Glutamine synthetase

Gene

GLN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-ammonia ligase activity Source: SGD

GO - Biological processi

  • glutamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YPR035W-MONOMER.
YEAST:YPR035W-MONOMER.
ReactomeiR-SCE-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-SCE-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Short name:
GS
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:GLN1
Ordered Locus Names:YPR035W
ORF Names:YP3085.01, YP9367.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR035W.
SGDiS000006239. GLN1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001531662 – 370Glutamine synthetaseAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei5PhosphoserineCombined sources1
Cross-linki283Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki324Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources2 Publications
Cross-linki363Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32288.
PRIDEiP32288.

2D gel databases

SWISS-2DPAGEP32288.

PTM databases

iPTMnetiP32288.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi36213. 43 interactors.
DIPiDIP-6699N.
IntActiP32288. 8 interactors.
MINTiMINT-658171.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 31Combined sources8
Beta strandi33 – 35Combined sources3
Beta strandi37 – 46Combined sources10
Helixi51 – 53Combined sources3
Beta strandi57 – 59Combined sources3
Turni61 – 65Combined sources5
Beta strandi74 – 83Combined sources10
Turni85 – 87Combined sources3
Beta strandi92 – 99Combined sources8
Beta strandi103 – 105Combined sources3
Helixi111 – 120Combined sources10
Helixi122 – 124Combined sources3
Beta strandi127 – 137Combined sources11
Beta strandi141 – 143Combined sources3
Beta strandi155 – 157Combined sources3
Turni164 – 166Combined sources3
Helixi170 – 183Combined sources14
Beta strandi187 – 192Combined sources6
Beta strandi198 – 207Combined sources10
Helixi209 – 227Combined sources19
Turni228 – 230Combined sources3
Beta strandi232 – 234Combined sources3
Beta strandi239 – 244Combined sources6
Beta strandi248 – 254Combined sources7
Helixi256 – 259Combined sources4
Beta strandi260 – 262Combined sources3
Helixi264 – 276Combined sources13
Helixi278 – 283Combined sources6
Helixi289 – 291Combined sources3
Beta strandi307 – 310Combined sources4
Beta strandi314 – 318Combined sources5
Helixi320 – 325Combined sources6
Beta strandi330 – 332Combined sources3
Helixi341 – 353Combined sources13
Turni361 – 363Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FKYX-ray2.95A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-370[»]
ProteinModelPortaliP32288.
SMRiP32288.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32288.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010047.
HOGENOMiHOG000061500.
InParanoidiP32288.
KOiK01915.
OMAiGIMCETV.
OrthoDBiEOG092C2OM1.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEASIEKTQ ILQKYLELDQ RGRIIAEYVW IDGTGNLRSK GRTLKKRITS
60 70 80 90 100
IDQLPEWNFD GSSTNQAPGH DSDIYLKPVA YYPDPFRRGD NIVVLAACYN
110 120 130 140 150
NDGTPNKFNH RHEAAKLFAA HKDEEIWFGL EQEYTLFDMY DDVYGWPKGG
160 170 180 190 200
YPAPQGPYYC GVGAGKVYAR DMIEAHYRAC LYAGLEISGI NAEVMPSQWE
210 220 230 240 250
FQVGPCTGID MGDQLWMARY FLHRVAEEFG IKISFHPKPL KGDWNGAGCH
260 270 280 290 300
TNVSTKEMRQ PGGMKYIEQA IEKLSKRHAE HIKLYGSDND MRLTGRHETA
310 320 330 340 350
SMTAFSSGVA NRGSSIRIPR SVAKEGYGYF EDRRPASNID PYLVTGIMCE
360 370
TVCGAIDNAD MTKEFERESS
Length:370
Mass (Da):41,766
Last modified:September 21, 2011 - v4
Checksum:i43139C40E97DB34D
GO

Sequence cautioni

The sequence AAA34644 differs from that shown. The submitted sequence does not correspond to the sequence published in the paper.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165G → D AA sequence (PubMed:2891705).Curated1
Sequence conflicti172M → V AA sequence (PubMed:2891705).Curated1
Sequence conflicti251T → A in CAA92141 (PubMed:9169875).Curated1
Sequence conflicti251T → A in CAA94985 (PubMed:9169875).Curated1
Sequence conflicti264M → T in CAA92141 (PubMed:9169875).Curated1
Sequence conflicti264M → T in CAA94985 (PubMed:9169875).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65157 Genomic DNA. Translation: AAA34644.1. Sequence problems.
Z68111 Genomic DNA. Translation: CAA92141.1.
Z71255 Genomic DNA. Translation: CAA94985.1.
Z49274 Genomic DNA. Translation: CAA89289.1.
BK006949 Genomic DNA. Translation: DAA11461.2.
PIRiS61058.
RefSeqiNP_015360.2. NM_001184132.2.

Genome annotation databases

EnsemblFungiiYPR035W; YPR035W; YPR035W.
GeneIDi856147.
KEGGisce:YPR035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65157 Genomic DNA. Translation: AAA34644.1. Sequence problems.
Z68111 Genomic DNA. Translation: CAA92141.1.
Z71255 Genomic DNA. Translation: CAA94985.1.
Z49274 Genomic DNA. Translation: CAA89289.1.
BK006949 Genomic DNA. Translation: DAA11461.2.
PIRiS61058.
RefSeqiNP_015360.2. NM_001184132.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FKYX-ray2.95A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-370[»]
ProteinModelPortaliP32288.
SMRiP32288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36213. 43 interactors.
DIPiDIP-6699N.
IntActiP32288. 8 interactors.
MINTiMINT-658171.

PTM databases

iPTMnetiP32288.

2D gel databases

SWISS-2DPAGEP32288.

Proteomic databases

MaxQBiP32288.
PRIDEiP32288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR035W; YPR035W; YPR035W.
GeneIDi856147.
KEGGisce:YPR035W.

Organism-specific databases

EuPathDBiFungiDB:YPR035W.
SGDiS000006239. GLN1.

Phylogenomic databases

GeneTreeiENSGT00390000010047.
HOGENOMiHOG000061500.
InParanoidiP32288.
KOiK01915.
OMAiGIMCETV.
OrthoDBiEOG092C2OM1.

Enzyme and pathway databases

BioCyciMetaCyc:YPR035W-MONOMER.
YEAST:YPR035W-MONOMER.
ReactomeiR-SCE-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-SCE-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

EvolutionaryTraceiP32288.
PROiP32288.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA_YEAST
AccessioniPrimary (citable) accession number: P32288
Secondary accession number(s): D6W445, Q03959
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 21, 2011
Last modified: November 2, 2016
This is version 155 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 346000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.