Reviewed,
UniProtKB/Swiss-Prot P32288 (GLNA_YEAST)
Last modified
June 16, 2009.
Version 91.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Glutamine synthetase Short name=GS EC=6.3.1.2 Alternative name(s): Glutamate--ammonia ligase | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 370 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine. |
| Subunit structure | Homooctamer. |
| Subcellular location | |
| Miscellaneous | Present with 346000 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the glutamine synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| PTM | Acetylation Isopeptide bond Ubl conjugation |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from direct assay. Source: SGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Ref.1Inferred from mutant phenotype. Source: SGD protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.3 | ||||||
| Chain | 2 – 370 | 369 | Glutamine synthetase | PRO_0000153166 | |||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.4 | ||||||
| Cross-link | 324 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6 Ref.7 | |||||||
Experimental info | |||||||||
| Sequence conflict | 165 | 1 | G → D AA sequence Ref.3 | ||||||
| Sequence conflict | 172 | 1 | M → V AA sequence Ref.3 | ||||||
| Sequence conflict | 251 | 1 | T → A in CAA92141. Ref.2 | ||||||
| Sequence conflict | 251 | 1 | T → A in CAA94985. Ref.2 | ||||||
| Sequence conflict | 264 | 1 | M → T in CAA92141. Ref.2 | ||||||
| Sequence conflict | 264 | 1 | M → T in CAA94985. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence of the GLN1 gene of Saccharomyces cerevisiae: role of the upstream region in regulation of glutamine synthetase expression." Minehart P.L., Magasanik B. J. Bacteriol. 174:1828-1836(1992) [PubMed: 1347768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.  Hani J.Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Sequence of peptides from Saccharomyces cerevisiae glutamine synthetase. N-terminal peptide and ATP-binding domain." Kim K.H., Rhee S.G. J. Biol. Chem. 263:833-838(1988) [PubMed: 2891705] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8; 149-166; 171-178; 220-224 AND 286-293. |
| [4] | "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT ALA-2. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-324, MASS SPECTROMETRY. |
| [7] | "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-324, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M65157 Genomic DNA. Translation: AAA34644.1. Sequence problems. Z68111 Genomic DNA. Translation: CAA92141.1. Z71255 Genomic DNA. Translation: CAA94985.1. Z49274 Genomic DNA. Translation: CAA89289.1. | |
| PIR | S61058. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LGR based on UniProtKB P06201. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6699N. |
| IntAct | P32288. 105 interactions. |
2-D gel databases | |
| SWISS-2DPAGE | P32288. |
Proteomic databases | |
| PeptideAtlas | P32288. |
| PRIDE | P32288. |
Genome annotation databases | |
| Ensembl | YPR035W. Saccharomyces cerevisiae. [Contig view] |
| GenomeReviews | Gene locus YPR035W in contig U00094_GR. |
| KEGG | sce:YPR035W. |
Organism-specific databases | |
| CYGD | YPR035w. |
| SGD | S000006239. GLN1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P32288. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MON-12439. |
| BRENDA | 6.3.1.2. 250. |
Gene expression databases | |
| ArrayExpress | P32288. |
| GermOnline | YPR035W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view] |
| Gene3D | G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit. |
| Pfam | PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view] |
| ProDom | PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GLNA_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32288 Secondary accession number(s): Q03959 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
