Reviewed, UniProtKB/Swiss-Prot P32282 (RIR1_BPT4)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase
    Protein B1

Gene names

Name:

NRDA

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 1 ATP-cone domain.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187226
Regions
	Domain	4 – 93	90	ATP-cone
Sites
	Active site	222	1	Hydrogen atom transfer By similarity
	Active site	435	1	Proton acceptor By similarity
	Active site	437	1	Proton acceptor By similarity
	Active site	439	1	Proton acceptor By similarity
	Active site	457	1	Hydrogen atom transfer By similarity
	Active site	726	1	Electron transfer By similarity
	Active site	727	1	Electron transfer By similarity
	Site	229	1	Allosteric effector binding By similarity
	Site	259	1	Allosteric effector binding By similarity
	Site	749	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	752	1	Interacts with thioredoxin/glutaredoxin By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32282-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 31D178EAA580068A	FASTA	754	85,973
	10 20 30 40 50 60 MQLINVIKSS GVSQSFDPQK IIKVLSWAAE GTSVDPYELY ENIKSYLRDG MTTDDIQTIV 70 80 90 100 110 120 IKAAANSISV EEPDYQYVAA RCLMFALRKH VYGQYEPRSF IDHISYCVNA GKYDPELLSK 130 140 150 160 170 180 YSAEEITFLE SKIKHERDME FTYSGAMQLK EKYLVKDKTT GQIYETPQFA FMTIGMALHQ 190 200 210 220 230 240 DEPVDRLKHV IRFYEAVSTR QISLPTPIMA GCRTPTRQFS SCVVIEAGDS LKSINKASAS 250 260 270 280 290 300 IVEYISKRAG IGINVGMIRA EGSKIGMGEV RHTGVIPFWK HFQTAVKSCS QGGIRGGAAT 310 320 330 340 350 360 AYYPIWHLEV ENLLVLKNNK GVEENRIRHM DYGVQLNDLM MERFGKNDYI TLFSPHEMGG 370 380 390 400 410 420 ELYYSYFKDQ DRFRELYEAA EKDPNIRKKR IKARELFELL MTERSGTARI YVQFIDNTNN 430 440 450 460 470 480 YTPFIREKAP IRQSNLCCEI AIPTNDVNSP DAEIGLCTLS AFVLDNFDWQ DQDKINELAE 490 500 510 520 530 540 VQVRALDNLL DYQGYPVPEA EKAKKRRNLG VGVTNYAAWL ASNFASYEDA NDLTHELFER 550 560 570 580 590 600 LQYGLIKASI KLAKEKGPSE YYSDTRWSRG ELPIDWYNKK IDQIAAPKYV CDWSALREDL 610 620 630 640 650 660 KLFGIRNSTL SALMPCESSS QVSNSTNGYE PPRGPVSVKE SKEGSFNQVV PNIEHNIDLY 670 680 690 700 710 720 DYTWKLAKKG NKPYLTQVAI MLKWVCQSAS ANTYYDPQIF PKGKVPMSIM IDDMLYGWYY 730 740 750 GIKNFYYHNT RDGSGTDDYE IETPKADDCA ACKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase." Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R. J. Biol. Chem. 263:16242-16251(1988) [PubMed: 2846540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Localization of the T4 phage ribonucleotide reductase B1 subunit gene and the nucleotide sequence of its upstream and 5' coding regions." Chu F.K., Maley G.F., Wang A.M., Maley F. Gene 57:143-148(1987) [PubMed: 3322944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-66.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03968 Genomic DNA. Translation: AAA32527.1. AF158101 Genomic DNA. Translation: AAD42621.1. M22627 Genomic DNA. Translation: AAA32531.1.
RefSeq	NP_049845.1.
3D structure databases
HSSP	HSSP built from PDB template 1R1R based on UniProtKB P00452.
ModBase	Search...
Genome annotation databases
GeneID	1258795.
GenomeReviews	Gene locus T4p235 in contig AF158101_GR.
Enzyme and pathway databases
BRENDA	1.17.4.1. 1108.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF03477. ATP-cone. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS51161. ATP_CONE. 1 hit. PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_BPT4

Accession

Primary (citable) accession number: P32282

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)