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P32282

- RIR1_BPT4

UniProt

P32282 - RIR1_BPT4

Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

NRDA

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Enzyme regulationi

    Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei206 – 2061SubstrateBy similarity
    Sitei222 – 2221Important for hydrogen atom transferBy similarity
    Sitei229 – 2291Allosteric effector bindingBy similarity
    Binding sitei250 – 2501Substrate; via amide nitrogenBy similarity
    Sitei259 – 2591Allosteric effector bindingBy similarity
    Active sitei435 – 4351Proton acceptorBy similarity
    Active sitei437 – 4371Cysteine radical intermediateBy similarity
    Active sitei439 – 4391Proton acceptorBy similarity
    Sitei457 – 4571Important for hydrogen atom transferBy similarity
    Sitei726 – 7261Important for electron transferBy similarity
    Sitei727 – 7271Important for electron transferBy similarity
    Sitei749 – 7491Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei752 – 7521Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
    Alternative name(s):
    Protein B1
    Ribonucleotide reductase
    Gene namesi
    Name:NRDA
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 754754Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi222 ↔ 457Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Structurei

    3D structure databases

    ProteinModelPortaliP32282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 9390ATP-conePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni221 – 2222Substrate bindingBy similarity
    Regioni435 – 4395Substrate bindingBy similarity
    Regioni615 – 6195Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 ATP-cone domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF03477. ATP-cone. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS51161. ATP_CONE. 1 hit.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32282-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLINVIKSS GVSQSFDPQK IIKVLSWAAE GTSVDPYELY ENIKSYLRDG    50
    MTTDDIQTIV IKAAANSISV EEPDYQYVAA RCLMFALRKH VYGQYEPRSF 100
    IDHISYCVNA GKYDPELLSK YSAEEITFLE SKIKHERDME FTYSGAMQLK 150
    EKYLVKDKTT GQIYETPQFA FMTIGMALHQ DEPVDRLKHV IRFYEAVSTR 200
    QISLPTPIMA GCRTPTRQFS SCVVIEAGDS LKSINKASAS IVEYISKRAG 250
    IGINVGMIRA EGSKIGMGEV RHTGVIPFWK HFQTAVKSCS QGGIRGGAAT 300
    AYYPIWHLEV ENLLVLKNNK GVEENRIRHM DYGVQLNDLM MERFGKNDYI 350
    TLFSPHEMGG ELYYSYFKDQ DRFRELYEAA EKDPNIRKKR IKARELFELL 400
    MTERSGTARI YVQFIDNTNN YTPFIREKAP IRQSNLCCEI AIPTNDVNSP 450
    DAEIGLCTLS AFVLDNFDWQ DQDKINELAE VQVRALDNLL DYQGYPVPEA 500
    EKAKKRRNLG VGVTNYAAWL ASNFASYEDA NDLTHELFER LQYGLIKASI 550
    KLAKEKGPSE YYSDTRWSRG ELPIDWYNKK IDQIAAPKYV CDWSALREDL 600
    KLFGIRNSTL SALMPCESSS QVSNSTNGYE PPRGPVSVKE SKEGSFNQVV 650
    PNIEHNIDLY DYTWKLAKKG NKPYLTQVAI MLKWVCQSAS ANTYYDPQIF 700
    PKGKVPMSIM IDDMLYGWYY GIKNFYYHNT RDGSGTDDYE IETPKADDCA 750
    ACKL 754
    Length:754
    Mass (Da):85,973
    Last modified:October 1, 1993 - v1
    Checksum:i31D178EAA580068A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03968 Genomic DNA. Translation: AAA32527.1.
    AF158101 Genomic DNA. Translation: AAD42621.1.
    M22627 Genomic DNA. Translation: AAA32531.1.
    RefSeqiNP_049845.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258795.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03968 Genomic DNA. Translation: AAA32527.1 .
    AF158101 Genomic DNA. Translation: AAD42621.1 .
    M22627 Genomic DNA. Translation: AAA32531.1 .
    RefSeqi NP_049845.1. NC_000866.4.

    3D structure databases

    ProteinModelPortali P32282.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258795.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF03477. ATP-cone. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS51161. ATP_CONE. 1 hit.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase."
      Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R.
      J. Biol. Chem. 263:16242-16251(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Localization of the T4 phage ribonucleotide reductase B1 subunit gene and the nucleotide sequence of its upstream and 5' coding regions."
      Chu F.K., Maley G.F., Wang A.M., Maley F.
      Gene 57:143-148(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-66.

    Entry informationi

    Entry nameiRIR1_BPT4
    AccessioniPrimary (citable) accession number: P32282
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3