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P32282

- RIR1_BPT4

UniProt

P32282 - RIR1_BPT4

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene
NRDA
Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei206 – 2061Substrate By similarity
Sitei222 – 2221Important for hydrogen atom transfer By similarity
Sitei229 – 2291Allosteric effector binding By similarity
Binding sitei250 – 2501Substrate; via amide nitrogen By similarity
Sitei259 – 2591Allosteric effector binding By similarity
Active sitei435 – 4351Proton acceptor By similarity
Active sitei437 – 4371Cysteine radical intermediate By similarity
Active sitei439 – 4391Proton acceptor By similarity
Sitei457 – 4571Important for hydrogen atom transfer By similarity
Sitei726 – 7261Important for electron transfer By similarity
Sitei727 – 7271Important for electron transfer By similarity
Sitei749 – 7491Interacts with thioredoxin/glutaredoxin By similarity
Sitei752 – 7521Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Protein B1
Ribonucleotide reductase
Gene namesi
Name:NRDA
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi222 ↔ 457Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliP32282.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 9390ATP-coneAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 2222Substrate binding By similarity
Regioni435 – 4395Substrate binding By similarity
Regioni615 – 6195Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32282-1 [UniParc]FASTAAdd to Basket

« Hide

MQLINVIKSS GVSQSFDPQK IIKVLSWAAE GTSVDPYELY ENIKSYLRDG    50
MTTDDIQTIV IKAAANSISV EEPDYQYVAA RCLMFALRKH VYGQYEPRSF 100
IDHISYCVNA GKYDPELLSK YSAEEITFLE SKIKHERDME FTYSGAMQLK 150
EKYLVKDKTT GQIYETPQFA FMTIGMALHQ DEPVDRLKHV IRFYEAVSTR 200
QISLPTPIMA GCRTPTRQFS SCVVIEAGDS LKSINKASAS IVEYISKRAG 250
IGINVGMIRA EGSKIGMGEV RHTGVIPFWK HFQTAVKSCS QGGIRGGAAT 300
AYYPIWHLEV ENLLVLKNNK GVEENRIRHM DYGVQLNDLM MERFGKNDYI 350
TLFSPHEMGG ELYYSYFKDQ DRFRELYEAA EKDPNIRKKR IKARELFELL 400
MTERSGTARI YVQFIDNTNN YTPFIREKAP IRQSNLCCEI AIPTNDVNSP 450
DAEIGLCTLS AFVLDNFDWQ DQDKINELAE VQVRALDNLL DYQGYPVPEA 500
EKAKKRRNLG VGVTNYAAWL ASNFASYEDA NDLTHELFER LQYGLIKASI 550
KLAKEKGPSE YYSDTRWSRG ELPIDWYNKK IDQIAAPKYV CDWSALREDL 600
KLFGIRNSTL SALMPCESSS QVSNSTNGYE PPRGPVSVKE SKEGSFNQVV 650
PNIEHNIDLY DYTWKLAKKG NKPYLTQVAI MLKWVCQSAS ANTYYDPQIF 700
PKGKVPMSIM IDDMLYGWYY GIKNFYYHNT RDGSGTDDYE IETPKADDCA 750
ACKL 754
Length:754
Mass (Da):85,973
Last modified:October 1, 1993 - v1
Checksum:i31D178EAA580068A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03968 Genomic DNA. Translation: AAA32527.1.
AF158101 Genomic DNA. Translation: AAD42621.1.
M22627 Genomic DNA. Translation: AAA32531.1.
RefSeqiNP_049845.1. NC_000866.4.

Genome annotation databases

GeneIDi1258795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03968 Genomic DNA. Translation: AAA32527.1 .
AF158101 Genomic DNA. Translation: AAD42621.1 .
M22627 Genomic DNA. Translation: AAA32531.1 .
RefSeqi NP_049845.1. NC_000866.4.

3D structure databases

ProteinModelPortali P32282.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258795.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase."
    Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R.
    J. Biol. Chem. 263:16242-16251(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Localization of the T4 phage ribonucleotide reductase B1 subunit gene and the nucleotide sequence of its upstream and 5' coding regions."
    Chu F.K., Maley G.F., Wang A.M., Maley F.
    Gene 57:143-148(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-66.

Entry informationi

Entry nameiRIR1_BPT4
AccessioniPrimary (citable) accession number: P32282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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