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Protein

ATP-dependent DNA helicase dda

Gene

dda

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 39ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase dda (EC:3.6.4.12)
Gene namesi
Name:dda
Synonyms:sud
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649251 – 439ATP-dependent DNA helicase ddaAdd BLAST439

Proteomic databases

PRIDEiP32270.

Interactioni

Subunit structurei

Interacts with UvsX and gene 32 protein.1 Publication

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 22Combined sources15
Beta strandi23 – 25Combined sources3
Beta strandi27 – 31Combined sources5
Helixi38 – 51Combined sources14
Beta strandi57 – 63Combined sources7
Helixi64 – 74Combined sources11
Beta strandi78 – 80Combined sources3
Helixi81 – 85Combined sources5
Beta strandi87 – 91Combined sources5
Beta strandi96 – 100Combined sources5
Beta strandi110 – 115Combined sources6
Helixi117 – 119Combined sources3
Helixi122 – 131Combined sources10
Beta strandi137 – 142Combined sources6
Helixi161 – 163Combined sources3
Beta strandi168 – 172Combined sources5
Helixi182 – 191Combined sources10
Beta strandi199 – 201Combined sources3
Beta strandi204 – 208Combined sources5
Beta strandi211 – 214Combined sources4
Helixi215 – 225Combined sources11
Beta strandi235 – 241Combined sources7
Helixi242 – 256Combined sources15
Beta strandi268 – 273Combined sources6
Beta strandi275 – 281Combined sources7
Beta strandi284 – 291Combined sources8
Beta strandi296 – 309Combined sources14
Beta strandi318 – 329Combined sources12
Beta strandi331 – 333Combined sources3
Beta strandi337 – 342Combined sources6
Helixi346 – 364Combined sources19
Beta strandi368 – 371Combined sources4
Helixi375 – 381Combined sources7
Beta strandi387 – 394Combined sources8
Helixi396 – 398Combined sources3
Beta strandi403 – 409Combined sources7
Helixi411 – 415Combined sources5
Helixi418 – 431Combined sources14
Beta strandi432 – 439Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UPUX-ray3.30A/B/C1-439[»]
ProteinModelPortaliP32270.
SMRiP32270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To E.coli recD (exonuclease V).Curated

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P32270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFDDLTEGQ KNAFNIVMKA IKEKKHHVTI NGPAGTGKTT LTKFIIEALI
60 70 80 90 100
STGGTGIILA APTHAAKKIL SKLSGKEAST IHSILKINPV TYEENVLFEQ
110 120 130 140 150
KEVPDLAKCR VLICDEVSMY DRKLFKILLS TIPPWCTIIG IGDNKQIRPV
160 170 180 190 200
EPGENTAYIS PFFTHKDFYQ CELTEVKRSN APIIDVATDV RNGKWNYDKV
210 220 230 240 250
VDGHGVRGFT GDTALRDFMV NYFSIVKSLD DLFENRVMAF TNKSVDKLNS
260 270 280 290 300
IIRKKIFETD KDFIVGEIIV MQEPLFKTYK IDGKPVSEII FNNGQLVRII
310 320 330 340 350
EAEYTSTFVK ARGVPGEYLI RHWDLTVETY GDDEYYREKI KIISSDEELY
360 370 380 390 400
KFNLFLAKTA ETYKNWNKGG KAPWSDFWDA KSQFSKVKAL PASTFHKAQG
410 420 430
MSVDRAFIYT PCIHYADVEL AQQLLYVGVT RGRYDVFYV
Length:439
Mass (Da):49,897
Last modified:December 19, 2001 - v2
Checksum:i2CDAC6AB99A5A583
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54G → E in AAA32488 (PubMed:1328208).Curated1
Sequence conflicti151E → D in AAA32488 (PubMed:1328208).Curated1
Sequence conflicti196N → I in AAA32488 (PubMed:1328208).Curated1
Sequence conflicti357A → G in AAA32488 (PubMed:1328208).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93048 Genomic DNA. Translation: AAA32488.1.
AF158101 Genomic DNA. Translation: AAD42555.1.
PIRiA45068.
RefSeqiNP_049632.1. NC_000866.4.

Genome annotation databases

GeneIDi1258784.
KEGGivg:1258784.

Cross-referencesi

Web resourcesi

Wikipedia

Dda (DNA-dependent ATPase) entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93048 Genomic DNA. Translation: AAA32488.1.
AF158101 Genomic DNA. Translation: AAD42555.1.
PIRiA45068.
RefSeqiNP_049632.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UPUX-ray3.30A/B/C1-439[»]
ProteinModelPortaliP32270.
SMRiP32270.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP32270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258784.
KEGGivg:1258784.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDA_BPT4
AccessioniPrimary (citable) accession number: P32270
Secondary accession number(s): Q9T0W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 19, 2001
Last modified: November 2, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.