Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase dda

Gene

dda

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 398ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase dda (EC:3.6.4.12)
Gene namesi
Name:dda
Synonyms:sud
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439ATP-dependent DNA helicase ddaPRO_0000164925Add
BLAST

Proteomic databases

PRIDEiP32270.

Interactioni

Subunit structurei

Interacts with UvsX and gene 32 protein.1 Publication

Structurei

Secondary structure

1
439
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2215Combined sources
Beta strandi23 – 253Combined sources
Beta strandi27 – 315Combined sources
Helixi38 – 5114Combined sources
Beta strandi57 – 637Combined sources
Helixi64 – 7411Combined sources
Beta strandi78 – 803Combined sources
Helixi81 – 855Combined sources
Beta strandi87 – 915Combined sources
Beta strandi96 – 1005Combined sources
Beta strandi110 – 1156Combined sources
Helixi117 – 1193Combined sources
Helixi122 – 13110Combined sources
Beta strandi137 – 1426Combined sources
Helixi161 – 1633Combined sources
Beta strandi168 – 1725Combined sources
Helixi182 – 19110Combined sources
Beta strandi199 – 2013Combined sources
Beta strandi204 – 2085Combined sources
Beta strandi211 – 2144Combined sources
Helixi215 – 22511Combined sources
Beta strandi235 – 2417Combined sources
Helixi242 – 25615Combined sources
Beta strandi268 – 2736Combined sources
Beta strandi275 – 2817Combined sources
Beta strandi284 – 2918Combined sources
Beta strandi296 – 30914Combined sources
Beta strandi318 – 32912Combined sources
Beta strandi331 – 3333Combined sources
Beta strandi337 – 3426Combined sources
Helixi346 – 36419Combined sources
Beta strandi368 – 3714Combined sources
Helixi375 – 3817Combined sources
Beta strandi387 – 3948Combined sources
Helixi396 – 3983Combined sources
Beta strandi403 – 4097Combined sources
Helixi411 – 4155Combined sources
Helixi418 – 43114Combined sources
Beta strandi432 – 4398Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UPUX-ray3.30A/B/C1-439[»]
ProteinModelPortaliP32270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To E.coli recD (exonuclease V).Curated

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P32270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFDDLTEGQ KNAFNIVMKA IKEKKHHVTI NGPAGTGKTT LTKFIIEALI
60 70 80 90 100
STGGTGIILA APTHAAKKIL SKLSGKEAST IHSILKINPV TYEENVLFEQ
110 120 130 140 150
KEVPDLAKCR VLICDEVSMY DRKLFKILLS TIPPWCTIIG IGDNKQIRPV
160 170 180 190 200
EPGENTAYIS PFFTHKDFYQ CELTEVKRSN APIIDVATDV RNGKWNYDKV
210 220 230 240 250
VDGHGVRGFT GDTALRDFMV NYFSIVKSLD DLFENRVMAF TNKSVDKLNS
260 270 280 290 300
IIRKKIFETD KDFIVGEIIV MQEPLFKTYK IDGKPVSEII FNNGQLVRII
310 320 330 340 350
EAEYTSTFVK ARGVPGEYLI RHWDLTVETY GDDEYYREKI KIISSDEELY
360 370 380 390 400
KFNLFLAKTA ETYKNWNKGG KAPWSDFWDA KSQFSKVKAL PASTFHKAQG
410 420 430
MSVDRAFIYT PCIHYADVEL AQQLLYVGVT RGRYDVFYV
Length:439
Mass (Da):49,897
Last modified:December 19, 2001 - v2
Checksum:i2CDAC6AB99A5A583
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541G → E in AAA32488 (PubMed:1328208).Curated
Sequence conflicti151 – 1511E → D in AAA32488 (PubMed:1328208).Curated
Sequence conflicti196 – 1961N → I in AAA32488 (PubMed:1328208).Curated
Sequence conflicti357 – 3571A → G in AAA32488 (PubMed:1328208).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93048 Genomic DNA. Translation: AAA32488.1.
AF158101 Genomic DNA. Translation: AAD42555.1.
PIRiA45068.
RefSeqiNP_049632.1. NC_000866.4.

Genome annotation databases

GeneIDi1258784.
KEGGivg:1258784.

Cross-referencesi

Web resourcesi

Wikipedia

Dda (DNA-dependent ATPase) entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93048 Genomic DNA. Translation: AAA32488.1.
AF158101 Genomic DNA. Translation: AAD42555.1.
PIRiA45068.
RefSeqiNP_049632.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UPUX-ray3.30A/B/C1-439[»]
ProteinModelPortaliP32270.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP32270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258784.
KEGGivg:1258784.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDA_BPT4
AccessioniPrimary (citable) accession number: P32270
Secondary accession number(s): Q9T0W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 19, 2001
Last modified: September 16, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.