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Protein

10 kDa anti-sigma factor

Gene

asiA

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
10 kDa anti-sigma factor
Alternative name(s):
10 kDa RNA polymerase-associated protein
Audrey Stevens' inhibitor
Gene namesi
Name:asiA
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649191 – 9010 kDa anti-sigma factorAdd BLAST90

Interactioni

Subunit structurei

Interacts with the host sigma factor RpoD, and thereby inhibits its interaction with the catalytic core of the host RNA polymerase.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rpoDP005795EBI-2124737,EBI-545104From a different organism.

Protein-protein interaction databases

DIPiDIP-60748N.
IntActiP32267. 4 interactors.

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 20Combined sources17
Helixi24 – 27Combined sources4
Helixi30 – 40Combined sources11
Beta strandi44 – 47Combined sources4
Helixi52 – 59Combined sources8
Helixi63 – 70Combined sources8
Beta strandi73 – 77Combined sources5
Turni78 – 82Combined sources5
Helixi83 – 87Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JR5NMR-A/B1-90[»]
1TKVNMR-A/B1-90[»]
1TL6NMR-A1-90[»]
1TLHNMR-A1-90[»]
ProteinModelPortaliP32267.
SMRiP32267.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32267.

Family & Domainsi

Family and domain databases

Gene3Di1.10.1810.10. 1 hit.
InterProiIPR015100. AsiA.
[Graphical view]
PfamiPF09010. AsiA. 1 hit.
[Graphical view]
SUPFAMiSSF69070. SSF69070. 1 hit.

Sequencei

Sequence statusi: Complete.

P32267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKNIDTVRE IITVASILIK FSREDIVENR ANFIAFLNEI GVTHEGRKLN
60 70 80 90
QNSFRKIVSE LTQEDKKTLI DEFNEGFEGV YRYLEMYTNK
Length:90
Mass (Da):10,590
Last modified:October 1, 1993 - v1
Checksum:i1802A150946F2D22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99441 Genomic DNA. Translation: AAA32480.1.
AF158101 Genomic DNA. Translation: AAD42539.1.
RefSeqiNP_049866.1. NC_000866.4.

Genome annotation databases

GeneIDi1258709.
KEGGivg:1258709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99441 Genomic DNA. Translation: AAA32480.1.
AF158101 Genomic DNA. Translation: AAD42539.1.
RefSeqiNP_049866.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JR5NMR-A/B1-90[»]
1TKVNMR-A/B1-90[»]
1TL6NMR-A1-90[»]
1TLHNMR-A1-90[»]
ProteinModelPortaliP32267.
SMRiP32267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60748N.
IntActiP32267. 4 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258709.
KEGGivg:1258709.

Miscellaneous databases

EvolutionaryTraceiP32267.

Family and domain databases

Gene3Di1.10.1810.10. 1 hit.
InterProiIPR015100. AsiA.
[Graphical view]
PfamiPF09010. AsiA. 1 hit.
[Graphical view]
SUPFAMiSSF69070. SSF69070. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASIA_BPT4
AccessioniPrimary (citable) accession number: P32267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.