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P32266 (MGM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynamin-like GTPase MGM1, mitochondrial
Alternative name(s):
Mitochondrial division and morphology protein 17
Mitochondrial genome maintenance protein 1

Cleaved into the following 2 chains:

  1. Dynamin-like GTPase MGM1 large isoform
    Short name=l-MGM1
  2. Dynamin-like GTPase MGM1 small isoform
    Short name=s-MGM1
Gene names
Name:MGM1
Synonyms:MDM17
Ordered Locus Names:YOR211C
ORF Names:YOR50-1
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length881 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane. Ref.7 Ref.8 Ref.9 Ref.11

Subunit structure

Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain. Ref.8 Ref.9 Ref.13

Subcellular location

Dynamin-like GTPase MGM1 large isoform: Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side Ref.7 Ref.9 Ref.12.

Dynamin-like GTPase MGM1 small isoform: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side Ref.7 Ref.9 Ref.12.

Post-translational modification

Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.

Miscellaneous

Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.

Sequence similarities

Belongs to the dynamin family.

Contains 1 GED domain.

Sequence caution

The sequence CAA44637.1 differs from that shown. Reason: Frameshift at position 4.

The sequence CAA63174.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA99426.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA99428.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainSignal-anchor
Transit peptide
Transmembrane
Transmembrane helix
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from sequence alignment PubMed 11038181. Source: GOC

membrane fusion

Inferred from direct assay PubMed 22977249. Source: SGD

mitochondrial fusion

Inferred from direct assay Ref.9. Source: UniProtKB

mitochondrial genome maintenance

Inferred from direct assay Ref.7. Source: UniProtKB

mitochondrial membrane organization

Inferred from direct assay Ref.9. Source: UniProtKB

mitochondrion inheritance

Inferred from direct assay Ref.7. Source: UniProtKB

mitochondrion organization

Inferred from mutant phenotype PubMed 15125685. Source: SGD

   Cellular_componentextrinsic component of mitochondrial inner membrane

Inferred from direct assay PubMed 11038181. Source: SGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intrinsic component of mitochondrial inner membrane

Inferred from direct assay PubMed 19505460. Source: SGD

mitochondrial crista

Inferred from direct assay PubMed 19505460. Source: SGD

mitochondrial inner boundary membrane

Inferred from direct assay PubMed 19505460. Source: SGD

mitochondrial intermembrane space

Inferred from direct assay Ref.7. Source: UniProtKB

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from sequence alignment PubMed 11038181. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FZO1P382972EBI-10865,EBI-20900
UGO1Q033272EBI-10865,EBI-32955

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5959Mitochondrion Ref.7
Chain60 – 881822Dynamin-like GTPase MGM1 large isoform
PRO_0000007400
Chain140 – 881742Dynamin-like GTPase MGM1 small isoform
PRO_0000007401

Regions

Topological domain60 – 7213Mitochondrial matrix Potential
Transmembrane73 – 9220Helical; Signal-anchor for type II membrane protein; Potential
Topological domain93 – 881789Mitochondrial intermembrane Potential
Domain780 – 87293GED
Nucleotide binding217 – 2248GTP Potential
Nucleotide binding317 – 3215GTP Potential
Nucleotide binding385 – 3884GTP Potential
Compositional bias149 – 17830Asp-rich (acidic)

Experimental info

Mutagenesis11M → A: Abolishes translation.
Mutagenesis391M → A: No effect on translation.
Mutagenesis791G → D or K: Loss of function; most MGM1 processed to s-MGM1. Ref.12
Mutagenesis811M → A: No effect on translation.
Mutagenesis921M → A: No effect on translation.
Mutagenesis2231K → A: Loss of stability. Ref.9
Mutagenesis2241S → A: Loss of GTPase activity. Ref.8 Ref.9
Mutagenesis2241S → N: Loss of GTPase activity. Ref.8 Ref.9
Mutagenesis2441T → A: Loss of GTPase activity. Ref.9
Mutagenesis2941E → K in mdm17; loss of function at 37 degrees Celsius. Ref.8
Mutagenesis8241R → A: Loss of GED function. Ref.9
Mutagenesis8541K → A: Loss of GED function. Ref.9
Sequence conflict41S → T in CAA44637. Ref.1
Sequence conflict1291G → C in CAA44637. Ref.1
Sequence conflict1701E → A in L07419. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P32266 [UniParc].

Last modified April 26, 2004. Version 3.
Checksum: 31524B40C5350096

FASTA88199,178
        10         20         30         40         50         60 
MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA LSFVITRRSI 

        70         80         90        100        110        120 
SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK DKLDRIKDLG ESMKEKFNKM 

       130        140        150        160        170        180 
FSGDKSQDGG HGNDGTVPTA TLIAATSLDD DESKRQGDPK DDDDEDDDDE DDENDSVDTT 

       190        200        210        220        230        240 
QDEMLNLTKQ MIEIRTILNK VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS 

       250        260        270        280        290        300 
NMVTRRPIEL TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP 

       310        320        330        340        350        360 
IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI ILAISAADVD 

       370        380        390        400        410        420 
LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN NKKYPLSMGY VGVITKTPSS 

       430        440        450        460        470        480 
INRKHLGLFG EAPSSSLSGI FSKGQHGQSS GEENTNGLKQ IVSHQFEKAY FKENKKYFTN 

       490        500        510        520        530        540 
CQVSTKKLRE KLIKILEISM SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN 

       550        560        570        580        590        600 
IDVLKLGIKE FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM 

       610        620        630        640        650        660 
LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI KDLVSNTAIN 

       670        680        690        700        710        720 
VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN LIKEELRQCN SRYQAIKNAV 

       730        740        750        760        770        780 
GSKKLANVMG YLENESNLQK ETLGMSKLLL ERGSEAIFLD KRCKVLSFRL KMLKNKCHST 

       790        800        810        820        830        840 
IEKDRCPEVF LSAVSDKLTS TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE 

       850        860        870        880 
DPKISRHIEL QKRKELLELA LEKIDSILVF KKSYKGVSKN L 

« Hide

References

« Hide 'large scale' references
[1]"Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin."
Jones B.A., Fangman W.L.
Genes Dev. 6:380-389(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BJ41-8C.
[2]"Normal mitochondrial structure and genome maintenance in yeast requires the dynamin-like product of the MGM1 gene."
Guan K., Farh L., Marshall T., Deschenes R.J.
Curr. Genet. 24:141-148(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
Galisson F., Dujon B.
Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-784.
Strain: ATCC 96604 / S288c / FY1679.
[6]"Interactions between three common subunits of yeast RNA polymerases I and III."
Lalo D., Carles C., Sentenac A., Thuriaux P.
Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 713-881.
Strain: ATCC 28383 / FL100 / VTT C-80102.
[7]"Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA."
Herlan M., Vogel F., Bornhoevd C., Neupert W., Reichert A.S.
J. Biol. Chem. 278:27781-27788(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 60-67 AND 140-147, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
Strain: ATCC 200060 / W303.
[8]"The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance."
Shepard K.A., Yaffe M.P.
J. Cell Biol. 144:711-720(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF SER-224 AND GLU-294, IDENTIFICATION OF THE TRANSLATION INITIATION CODON.
[9]"The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion."
Wong E.D., Wagner J.A., Scott S.V., Okreglak V., Holewinske T.J., Cassidy-Stone A., Nunnari J.
J. Cell Biol. 160:303-311(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-223; SER-224; THR-244; ARG-824 AND LYS-854.
[10]"Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane."
Sesaki H., Southard S.M., Yaffe M.P., Jensen R.E.
Mol. Biol. Cell 14:2342-2356(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FZO1 AND UGO1.
[11]"Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion."
Sesaki H., Jensen R.E.
J. Biol. Chem. 279:28298-28303(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH UGO1.
[12]"Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor."
Herlan M., Bornhoevd C., Hell K., Neupert W., Reichert A.S.
J. Cell Biol. 165:167-173(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-79.
[13]"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62834 Genomic DNA. Translation: CAA44637.1. Frameshift.
L07419 Genomic DNA. No translation available.
Z75119 Genomic DNA. Translation: CAA99426.1. Different initiation.
Z75120 Genomic DNA. Translation: CAA99428.1. Different initiation.
X92441 Genomic DNA. Translation: CAA63174.1. Different initiation.
L11274 Genomic DNA. Translation: AAB59316.1.
BK006948 Genomic DNA. Translation: DAA10983.1.
PIRS33918.
RefSeqNP_014854.2. NM_001183630.1.

3D structure databases

ProteinModelPortalP32266.
SMRP32266. Positions 182-864.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34606. 91 interactions.
DIPDIP-8008N.
IntActP32266. 6 interactions.
MINTMINT-1354456.
STRING4932.YOR211C.

Protein family/group databases

TCDB9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

Proteomic databases

PaxDbP32266.
PeptideAtlasP32266.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR211C; YOR211C; YOR211C.
GeneID854386.
KEGGsce:YOR211C.

Organism-specific databases

SGDS000005737. MGM1.

Phylogenomic databases

eggNOGCOG0699.
GeneTreeENSGT00550000074851.
HOGENOMHOG000206760.
OMAYVGVVCK.
OrthoDBEOG7ZD23R.

Enzyme and pathway databases

BioCycYEAST:G3O-33713-MONOMER.

Gene expression databases

GenevestigatorP32266.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11566. PTHR11566. 1 hit.
PfamPF00350. Dynamin_N. 1 hit.
[Graphical view]
PRINTSPR00195. DYNAMIN.
SMARTSM00053. DYNc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00410. DYNAMIN. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976535.
PMAP-CutDBP32266.

Entry information

Entry nameMGM1_YEAST
AccessionPrimary (citable) accession number: P32266
Secondary accession number(s): D6W2R7, Q02609, Q08627
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 26, 2004
Last modified: April 16, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families