Dynamin-like GTPase MGM1, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32266 (MGM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dynamin-like GTPase MGM1, mitochondrial

Alternative name(s):
Mitochondrial division and morphology protein 17
Mitochondrial genome maintenance protein 1

Cleaved into the following 2 chains:

Dynamin-like GTPase MGM1 large isoform
Short name=l-MGM1
Dynamin-like GTPase MGM1 small isoform
Short name=s-MGM1

Gene names

Name:	MGM1
Synonyms:	MDM17
Ordered Locus Names:	YOR211C
ORF Names:	YOR50-1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane. Ref.7 Ref.8 Ref.9 Ref.11
Subunit structure	Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain. Ref.8 Ref.9 Ref.13
Subcellular location	Dynamin-like GTPase MGM1 large isoform: Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side Ref.7 Ref.9 Ref.12. Dynamin-like GTPase MGM1 small isoform: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side Ref.7 Ref.9 Ref.12.
Post-translational modification	Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.
Miscellaneous	Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain.
Sequence caution	The sequence CAA44637.1 differs from that shown. Reason: Frameshift at position 4. The sequence CAA63174.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA99426.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA99428.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Signal-anchor Transit peptide Transmembrane Transmembrane helix
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial fusion Inferred from direct assay Ref.9. Source: UniProtKB mitochondrial genome maintenance Inferred from direct assay Ref.7. Source: UniProtKB mitochondrial membrane organization Inferred from direct assay Ref.9. Source: UniProtKB mitochondrion inheritance Inferred from direct assay Ref.7. Source: UniProtKB
Cellular component	extrinsic to mitochondrial inner membrane Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to mitochondrial inner membrane Inferred from direct assay. Source: SGD mitochondrial crista Inferred from direct assay. Source: SGD mitochondrial inner boundary membrane Inferred from direct assay. Source: SGD mitochondrial intermembrane space Inferred from direct assay Ref.7. Source: UniProtKB
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.11. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FZO1	P38297	2	EBI-10865,EBI-20900
UGO1	Q03327	2	EBI-10865,EBI-32955

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 59

Mitochondrion Ref.7

Chain

60 – 881

822

Dynamin-like GTPase MGM1 large isoform

PRO_0000007400

Chain

140 – 881

742

Dynamin-like GTPase MGM1 small isoform

PRO_0000007401

Regions

Topological domain

60 – 72

Mitochondrial matrix Potential

Transmembrane

73 – 92

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

93 – 881

789

Mitochondrial intermembrane Potential

Domain

780 – 872

GED

Nucleotide binding

217 – 224

GTP Potential

Nucleotide binding

317 – 321

GTP Potential

Nucleotide binding

385 – 388

GTP Potential

Compositional bias

149 – 178

Asp-rich (acidic)

Experimental info

Mutagenesis

M → A: Abolishes translation.

Mutagenesis

M → A: No effect on translation.

Mutagenesis

G → D or K: Loss of function; most MGM1 processed to s-MGM1. Ref.12

Mutagenesis

M → A: No effect on translation.

Mutagenesis

M → A: No effect on translation.

Mutagenesis

223

K → A: Loss of stability. Ref.9

Mutagenesis

224

S → A: Loss of GTPase activity. Ref.8 Ref.9

Mutagenesis

224

S → N: Loss of GTPase activity. Ref.8 Ref.9

Mutagenesis

244

T → A: Loss of GTPase activity. Ref.9

Mutagenesis

294

E → K in mdm17; loss of function at 37 degrees Celsius. Ref.8

Mutagenesis

824

R → A: Loss of GED function. Ref.9

Mutagenesis

854

K → A: Loss of GED function. Ref.9

Sequence conflict

S → T in CAA44637. Ref.1

Sequence conflict

129

G → C in CAA44637. Ref.1

Sequence conflict

170

E → A in L07419. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P32266 [UniParc].

Last modified April 26, 2004. Version 3.
Checksum: 31524B40C5350096
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FASTA

881

99,178

        10         20         30         40         50         60 
MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA LSFVITRRSI 

        70         80         90        100        110        120 
SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK DKLDRIKDLG ESMKEKFNKM 

       130        140        150        160        170        180 
FSGDKSQDGG HGNDGTVPTA TLIAATSLDD DESKRQGDPK DDDDEDDDDE DDENDSVDTT 

       190        200        210        220        230        240 
QDEMLNLTKQ MIEIRTILNK VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS 

       250        260        270        280        290        300 
NMVTRRPIEL TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP 

       310        320        330        340        350        360 
IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI ILAISAADVD 

       370        380        390        400        410        420 
LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN NKKYPLSMGY VGVITKTPSS 

       430        440        450        460        470        480 
INRKHLGLFG EAPSSSLSGI FSKGQHGQSS GEENTNGLKQ IVSHQFEKAY FKENKKYFTN 

       490        500        510        520        530        540 
CQVSTKKLRE KLIKILEISM SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN 

       550        560        570        580        590        600 
IDVLKLGIKE FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM 

       610        620        630        640        650        660 
LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI KDLVSNTAIN 

       670        680        690        700        710        720 
VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN LIKEELRQCN SRYQAIKNAV 

       730        740        750        760        770        780 
GSKKLANVMG YLENESNLQK ETLGMSKLLL ERGSEAIFLD KRCKVLSFRL KMLKNKCHST 

       790        800        810        820        830        840 
IEKDRCPEVF LSAVSDKLTS TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE 

       850        860        870        880 
DPKISRHIEL QKRKELLELA LEKIDSILVF KKSYKGVSKN L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin." Jones B.A., Fangman W.L. Genes Dev. 6:380-389(1992) [PubMed: 1532158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BJ41-8C.
[2]	"Normal mitochondrial structure and genome maintenance in yeast requires the dynamin-like product of the MGM1 gene." Guan K., Farh L., Marshall T., Deschenes R.J. Curr. Genet. 24:141-148(1993) [PubMed: 7916673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae." Galisson F., Dujon B. Yeast 12:877-885(1996) [PubMed: 8840505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-784. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Interactions between three common subunits of yeast RNA polymerases I and III." Lalo D., Carles C., Sentenac A., Thuriaux P. Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed: 8516295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 713-881. Strain: ATCC 28383 / FL100 / VTT C-80102.
[7]	"Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA." Herlan M., Vogel F., Bornhoevd C., Neupert W., Reichert A.S. J. Biol. Chem. 278:27781-27788(2003) [PubMed: 12707284] [Abstract] Cited for: PROTEIN SEQUENCE OF 60-67 AND 140-147, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY. Strain: ATCC 200060 / W303.
[8]	"The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance." Shepard K.A., Yaffe M.P. J. Cell Biol. 144:711-720(1999) [PubMed: 10037792] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF SER-224 AND GLU-294, IDENTIFICATION OF THE TRANSLATION INITIATION CODON.
[9]	"The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion." Wong E.D., Wagner J.A., Scott S.V., Okreglak V., Holewinske T.J., Cassidy-Stone A., Nunnari J. J. Cell Biol. 160:303-311(2003) [PubMed: 12566426] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-223; SER-224; THR-244; ARG-824 AND LYS-854.
[10]	"Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane." Sesaki H., Southard S.M., Yaffe M.P., Jensen R.E. Mol. Biol. Cell 14:2342-2356(2003) [PubMed: 12808034] [Abstract] Cited for: INTERACTION WITH FZO1 AND UGO1.
[11]	"Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion." Sesaki H., Jensen R.E. J. Biol. Chem. 279:28298-28303(2004) [PubMed: 15087460] [Abstract] Cited for: FUNCTION, INTERACTION WITH UGO1.
[12]	"Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor." Herlan M., Bornhoevd C., Hell K., Neupert W., Reichert A.S. J. Cell Biol. 165:167-173(2004) [PubMed: 15096522] [Abstract] Cited for: PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-79.
[13]	"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins." Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C. Mol. Biol. Cell 17:1436-1450(2006) [PubMed: 16407407] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62834 Genomic DNA. Translation: CAA44637.1. Frameshift. L07419 Genomic DNA. No translation available. Z75119 Genomic DNA. Translation: CAA99426.1. Different initiation. Z75120 Genomic DNA. Translation: CAA99428.1. Different initiation. X92441 Genomic DNA. Translation: CAA63174.1. Different initiation. L11274 Genomic DNA. Translation: AAB59316.1. BK006948 Genomic DNA. Translation: DAA10983.1.
PIR	S33918.
RefSeq	NP_014854.2. NM_001183630.1.
3D structure databases
ProteinModelPortal	P32266.
SMR	P32266. Positions 182-864.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8008N.
IntAct	P32266. 37 interactions.
MINT	MINT-1354456.
STRING	P32266.
Protein family/group databases
TCDB	9.B.25.1.1. mitochondrial inner/outer membrane fusion (MMF) family.
Proteomic databases
PeptideAtlas	P32266.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR211C; YOR211C; YOR211C.
GeneID	854386.
KEGG	sce:YOR211C.
NMPDR	fig\|4932.3.peg.5966.
Organism-specific databases
SGD	S000005737. MGM1.
Phylogenomic databases
eggNOG	fuNOG05268.
GeneTree	EFGT00050000001920.
HOGENOM	HBG333387.
OMA	GYVGVIT.
OrthoDB	EOG4HX88B.
Gene expression databases
ArrayExpress	P32266.
Genevestigator	P32266.
GermOnline	YOR211C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022812. Dynamin. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR020850. GTPase_effector_domain_GED. [Graphical view]
Pfam	PF00350. Dynamin_N. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976535.
PMAP-CutDB	P32266.

Entry information

Entry name

MGM1_YEAST

Accession

Primary (citable) accession number: P32266
Secondary accession number(s): D6W2R7, Q02609, Q08627

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	April 26, 2004
Last modified:	January 25, 2012
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents