Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32266

- MGM1_YEAST

UniProt

P32266 - MGM1_YEAST

Protein

Dynamin-like GTPase MGM1, mitochondrial

Gene

MGM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 3 (26 Apr 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi217 – 2248GTPSequence Analysis
    Nucleotide bindingi317 – 3215GTPSequence Analysis
    Nucleotide bindingi385 – 3884GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. membrane fusion Source: SGD
    3. mitochondrial fusion Source: UniProtKB
    4. mitochondrial genome maintenance Source: UniProtKB
    5. mitochondrial membrane organization Source: UniProtKB
    6. mitochondrion inheritance Source: UniProtKB
    7. mitochondrion organization Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33713-MONOMER.

    Protein family/group databases

    TCDBi9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynamin-like GTPase MGM1, mitochondrial
    Alternative name(s):
    Mitochondrial division and morphology protein 17
    Mitochondrial genome maintenance protein 1
    Cleaved into the following 2 chains:
    Gene namesi
    Name:MGM1
    Synonyms:MDM17
    Ordered Locus Names:YOR211C
    ORF Names:YOR50-1
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    SGDiS000005737. MGM1.

    Subcellular locationi

    GO - Cellular componenti

    1. extrinsic component of mitochondrial inner membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. intrinsic component of mitochondrial inner membrane Source: SGD
    4. mitochondrial crista Source: SGD
    5. mitochondrial inner boundary membrane Source: SGD
    6. mitochondrial intermembrane space Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1 – 11M → A: Abolishes translation.
    Mutagenesisi39 – 391M → A: No effect on translation.
    Mutagenesisi79 – 791G → D or K: Loss of function; most MGM1 processed to s-MGM1. 1 Publication
    Mutagenesisi81 – 811M → A: No effect on translation.
    Mutagenesisi92 – 921M → A: No effect on translation.
    Mutagenesisi223 – 2231K → A: Loss of stability. 1 Publication
    Mutagenesisi224 – 2241S → A: Loss of GTPase activity. 2 Publications
    Mutagenesisi224 – 2241S → N: Loss of GTPase activity. 2 Publications
    Mutagenesisi244 – 2441T → A: Loss of GTPase activity. 1 Publication
    Mutagenesisi294 – 2941E → K in mdm17; loss of function at 37 degrees Celsius. 1 Publication
    Mutagenesisi824 – 8241R → A: Loss of GED function. 1 Publication
    Mutagenesisi854 – 8541K → A: Loss of GED function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5959Mitochondrion1 PublicationAdd
    BLAST
    Chaini60 – 881822Dynamin-like GTPase MGM1 large isoformPRO_0000007400Add
    BLAST
    Chaini140 – 881742Dynamin-like GTPase MGM1 small isoformPRO_0000007401Add
    BLAST

    Post-translational modificationi

    Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.1 Publication

    Proteomic databases

    MaxQBiP32266.
    PaxDbiP32266.
    PeptideAtlasiP32266.

    Miscellaneous databases

    PMAP-CutDBP32266.

    Expressioni

    Gene expression databases

    GenevestigatoriP32266.

    Interactioni

    Subunit structurei

    Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FZO1P382972EBI-10865,EBI-20900
    UGO1Q033272EBI-10865,EBI-32955

    Protein-protein interaction databases

    BioGridi34606. 92 interactions.
    DIPiDIP-8008N.
    IntActiP32266. 6 interactions.
    MINTiMINT-1354456.
    STRINGi4932.YOR211C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32266.
    SMRiP32266. Positions 205-424.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini60 – 7213Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini93 – 881789Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei73 – 9220Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini207 – 505299Dynamin-type GAdd
    BLAST
    Domaini780 – 87293GEDPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi149 – 17830Asp-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 1 GED domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0699.
    GeneTreeiENSGT00550000074851.
    HOGENOMiHOG000206760.
    OMAiYVGVVCK.
    OrthoDBiEOG7ZD23R.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11566. PTHR11566. 1 hit.
    PfamiPF00350. Dynamin_N. 1 hit.
    [Graphical view]
    PRINTSiPR00195. DYNAMIN.
    SMARTiSM00053. DYNc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32266-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA    50
    LSFVITRRSI SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK 100
    DKLDRIKDLG ESMKEKFNKM FSGDKSQDGG HGNDGTVPTA TLIAATSLDD 150
    DESKRQGDPK DDDDEDDDDE DDENDSVDTT QDEMLNLTKQ MIEIRTILNK 200
    VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS NMVTRRPIEL 250
    TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP 300
    IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI 350
    ILAISAADVD LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN 400
    NKKYPLSMGY VGVITKTPSS INRKHLGLFG EAPSSSLSGI FSKGQHGQSS 450
    GEENTNGLKQ IVSHQFEKAY FKENKKYFTN CQVSTKKLRE KLIKILEISM 500
    SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN IDVLKLGIKE 550
    FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM 600
    LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI 650
    KDLVSNTAIN VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN 700
    LIKEELRQCN SRYQAIKNAV GSKKLANVMG YLENESNLQK ETLGMSKLLL 750
    ERGSEAIFLD KRCKVLSFRL KMLKNKCHST IEKDRCPEVF LSAVSDKLTS 800
    TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE DPKISRHIEL 850
    QKRKELLELA LEKIDSILVF KKSYKGVSKN L 881
    Length:881
    Mass (Da):99,178
    Last modified:April 26, 2004 - v3
    Checksum:i31524B40C5350096
    GO

    Sequence cautioni

    The sequence CAA44637.1 differs from that shown. Reason: Frameshift at position 4.
    The sequence CAA63174.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA99426.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA99428.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41S → T in CAA44637. (PubMed:1532158)Curated
    Sequence conflicti129 – 1291G → C in CAA44637. (PubMed:1532158)Curated
    Sequence conflicti170 – 1701E → A in L07419. (PubMed:7916673)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62834 Genomic DNA. Translation: CAA44637.1. Frameshift.
    L07419 Genomic DNA. No translation available.
    Z75119 Genomic DNA. Translation: CAA99426.1. Different initiation.
    Z75120 Genomic DNA. Translation: CAA99428.1. Different initiation.
    X92441 Genomic DNA. Translation: CAA63174.1. Different initiation.
    L11274 Genomic DNA. Translation: AAB59316.1.
    BK006948 Genomic DNA. Translation: DAA10983.1.
    PIRiS33918.
    RefSeqiNP_014854.2. NM_001183630.1.

    Genome annotation databases

    EnsemblFungiiYOR211C; YOR211C; YOR211C.
    GeneIDi854386.
    KEGGisce:YOR211C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62834 Genomic DNA. Translation: CAA44637.1 . Frameshift.
    L07419 Genomic DNA. No translation available.
    Z75119 Genomic DNA. Translation: CAA99426.1 . Different initiation.
    Z75120 Genomic DNA. Translation: CAA99428.1 . Different initiation.
    X92441 Genomic DNA. Translation: CAA63174.1 . Different initiation.
    L11274 Genomic DNA. Translation: AAB59316.1 .
    BK006948 Genomic DNA. Translation: DAA10983.1 .
    PIRi S33918.
    RefSeqi NP_014854.2. NM_001183630.1.

    3D structure databases

    ProteinModelPortali P32266.
    SMRi P32266. Positions 205-424.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34606. 92 interactions.
    DIPi DIP-8008N.
    IntActi P32266. 6 interactions.
    MINTi MINT-1354456.
    STRINGi 4932.YOR211C.

    Protein family/group databases

    TCDBi 9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

    Proteomic databases

    MaxQBi P32266.
    PaxDbi P32266.
    PeptideAtlasi P32266.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR211C ; YOR211C ; YOR211C .
    GeneIDi 854386.
    KEGGi sce:YOR211C.

    Organism-specific databases

    SGDi S000005737. MGM1.

    Phylogenomic databases

    eggNOGi COG0699.
    GeneTreei ENSGT00550000074851.
    HOGENOMi HOG000206760.
    OMAi YVGVVCK.
    OrthoDBi EOG7ZD23R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33713-MONOMER.

    Miscellaneous databases

    NextBioi 976535.
    PMAP-CutDB P32266.

    Gene expression databases

    Genevestigatori P32266.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11566. PTHR11566. 1 hit.
    Pfami PF00350. Dynamin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00195. DYNAMIN.
    SMARTi SM00053. DYNc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin."
      Jones B.A., Fangman W.L.
      Genes Dev. 6:380-389(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BJ41-8C.
    2. "Normal mitochondrial structure and genome maintenance in yeast requires the dynamin-like product of the MGM1 gene."
      Guan K., Farh L., Marshall T., Deschenes R.J.
      Curr. Genet. 24:141-148(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
      Galisson F., Dujon B.
      Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-784.
      Strain: ATCC 96604 / S288c / FY1679.
    6. "Interactions between three common subunits of yeast RNA polymerases I and III."
      Lalo D., Carles C., Sentenac A., Thuriaux P.
      Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 713-881.
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    7. "Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA."
      Herlan M., Vogel F., Bornhoevd C., Neupert W., Reichert A.S.
      J. Biol. Chem. 278:27781-27788(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 60-67 AND 140-147, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
      Strain: ATCC 200060 / W303.
    8. "The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance."
      Shepard K.A., Yaffe M.P.
      J. Cell Biol. 144:711-720(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF SER-224 AND GLU-294, IDENTIFICATION OF THE TRANSLATION INITIATION CODON.
    9. "The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion."
      Wong E.D., Wagner J.A., Scott S.V., Okreglak V., Holewinske T.J., Cassidy-Stone A., Nunnari J.
      J. Cell Biol. 160:303-311(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-223; SER-224; THR-244; ARG-824 AND LYS-854.
    10. "Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane."
      Sesaki H., Southard S.M., Yaffe M.P., Jensen R.E.
      Mol. Biol. Cell 14:2342-2356(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FZO1 AND UGO1.
    11. "Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion."
      Sesaki H., Jensen R.E.
      J. Biol. Chem. 279:28298-28303(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH UGO1.
    12. "Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor."
      Herlan M., Bornhoevd C., Hell K., Neupert W., Reichert A.S.
      J. Cell Biol. 165:167-173(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-79.
    13. "Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
      Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
      Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiMGM1_YEAST
    AccessioniPrimary (citable) accession number: P32266
    Secondary accession number(s): D6W2R7, Q02609, Q08627
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 133 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3