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P32266

- MGM1_YEAST

UniProt

P32266 - MGM1_YEAST

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Protein

Dynamin-like GTPase MGM1, mitochondrial

Gene
MGM1, MDM17, YOR211C, YOR50-1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi217 – 2248GTP Reviewed prediction
Nucleotide bindingi317 – 3215GTP Reviewed prediction
Nucleotide bindingi385 – 3884GTP Reviewed prediction

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. membrane fusion Source: SGD
  3. mitochondrial fusion Source: UniProtKB
  4. mitochondrial genome maintenance Source: UniProtKB
  5. mitochondrial membrane organization Source: UniProtKB
  6. mitochondrion inheritance Source: UniProtKB
  7. mitochondrion organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33713-MONOMER.

Protein family/group databases

TCDBi9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-like GTPase MGM1, mitochondrial
Alternative name(s):
Mitochondrial division and morphology protein 17
Mitochondrial genome maintenance protein 1
Cleaved into the following 2 chains:
Gene namesi
Name:MGM1
Synonyms:MDM17
Ordered Locus Names:YOR211C
ORF Names:YOR50-1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

SGDiS000005737. MGM1.

Subcellular locationi

Chain Dynamin-like GTPase MGM1 large isoform : Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side 3 Publications
Chain Dynamin-like GTPase MGM1 small isoform : Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side 3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini60 – 7213Mitochondrial matrix Reviewed predictionAdd
BLAST
Transmembranei73 – 9220Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini93 – 881789Mitochondrial intermembrane Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. extrinsic component of mitochondrial inner membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. intrinsic component of mitochondrial inner membrane Source: SGD
  4. mitochondrial crista Source: SGD
  5. mitochondrial inner boundary membrane Source: SGD
  6. mitochondrial intermembrane space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 11M → A: Abolishes translation.
Mutagenesisi39 – 391M → A: No effect on translation.
Mutagenesisi79 – 791G → D or K: Loss of function; most MGM1 processed to s-MGM1. 1 Publication
Mutagenesisi81 – 811M → A: No effect on translation.
Mutagenesisi92 – 921M → A: No effect on translation.
Mutagenesisi223 – 2231K → A: Loss of stability. 1 Publication
Mutagenesisi224 – 2241S → A: Loss of GTPase activity. 2 Publications
Mutagenesisi224 – 2241S → N: Loss of GTPase activity. 2 Publications
Mutagenesisi244 – 2441T → A: Loss of GTPase activity. 1 Publication
Mutagenesisi294 – 2941E → K in mdm17; loss of function at 37 degrees Celsius. 1 Publication
Mutagenesisi824 – 8241R → A: Loss of GED function. 1 Publication
Mutagenesisi854 – 8541K → A: Loss of GED function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5959Mitochondrion1 PublicationAdd
BLAST
Chaini60 – 881822Dynamin-like GTPase MGM1 large isoformPRO_0000007400Add
BLAST
Chaini140 – 881742Dynamin-like GTPase MGM1 small isoformPRO_0000007401Add
BLAST

Post-translational modificationi

Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.

Proteomic databases

MaxQBiP32266.
PaxDbiP32266.
PeptideAtlasiP32266.

Miscellaneous databases

PMAP-CutDBP32266.

Expressioni

Gene expression databases

GenevestigatoriP32266.

Interactioni

Subunit structurei

Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FZO1P382972EBI-10865,EBI-20900
UGO1Q033272EBI-10865,EBI-32955

Protein-protein interaction databases

BioGridi34606. 91 interactions.
DIPiDIP-8008N.
IntActiP32266. 6 interactions.
MINTiMINT-1354456.
STRINGi4932.YOR211C.

Structurei

3D structure databases

ProteinModelPortaliP32266.
SMRiP32266. Positions 205-424.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 505299Dynamin-type GAdd
BLAST
Domaini780 – 87293GEDAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi149 – 17830Asp-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 GED domain.

Keywords - Domaini

Signal-anchor, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0699.
GeneTreeiENSGT00550000074851.
HOGENOMiHOG000206760.
OMAiYVGVVCK.
OrthoDBiEOG7ZD23R.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32266-1 [UniParc]FASTAAdd to Basket

« Hide

MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA    50
LSFVITRRSI SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK 100
DKLDRIKDLG ESMKEKFNKM FSGDKSQDGG HGNDGTVPTA TLIAATSLDD 150
DESKRQGDPK DDDDEDDDDE DDENDSVDTT QDEMLNLTKQ MIEIRTILNK 200
VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS NMVTRRPIEL 250
TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP 300
IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI 350
ILAISAADVD LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN 400
NKKYPLSMGY VGVITKTPSS INRKHLGLFG EAPSSSLSGI FSKGQHGQSS 450
GEENTNGLKQ IVSHQFEKAY FKENKKYFTN CQVSTKKLRE KLIKILEISM 500
SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN IDVLKLGIKE 550
FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM 600
LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI 650
KDLVSNTAIN VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN 700
LIKEELRQCN SRYQAIKNAV GSKKLANVMG YLENESNLQK ETLGMSKLLL 750
ERGSEAIFLD KRCKVLSFRL KMLKNKCHST IEKDRCPEVF LSAVSDKLTS 800
TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE DPKISRHIEL 850
QKRKELLELA LEKIDSILVF KKSYKGVSKN L 881
Length:881
Mass (Da):99,178
Last modified:April 26, 2004 - v3
Checksum:i31524B40C5350096
GO

Sequence cautioni

The sequence CAA44637.1 differs from that shown. Reason: Frameshift at position 4.
The sequence CAA63174.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA99426.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA99428.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41S → T in CAA44637. 1 Publication
Sequence conflicti129 – 1291G → C in CAA44637. 1 Publication
Sequence conflicti170 – 1701E → A in L07419. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62834 Genomic DNA. Translation: CAA44637.1. Frameshift.
L07419 Genomic DNA. No translation available.
Z75119 Genomic DNA. Translation: CAA99426.1. Different initiation.
Z75120 Genomic DNA. Translation: CAA99428.1. Different initiation.
X92441 Genomic DNA. Translation: CAA63174.1. Different initiation.
L11274 Genomic DNA. Translation: AAB59316.1.
BK006948 Genomic DNA. Translation: DAA10983.1.
PIRiS33918.
RefSeqiNP_014854.2. NM_001183630.1.

Genome annotation databases

EnsemblFungiiYOR211C; YOR211C; YOR211C.
GeneIDi854386.
KEGGisce:YOR211C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62834 Genomic DNA. Translation: CAA44637.1 . Frameshift.
L07419 Genomic DNA. No translation available.
Z75119 Genomic DNA. Translation: CAA99426.1 . Different initiation.
Z75120 Genomic DNA. Translation: CAA99428.1 . Different initiation.
X92441 Genomic DNA. Translation: CAA63174.1 . Different initiation.
L11274 Genomic DNA. Translation: AAB59316.1 .
BK006948 Genomic DNA. Translation: DAA10983.1 .
PIRi S33918.
RefSeqi NP_014854.2. NM_001183630.1.

3D structure databases

ProteinModelPortali P32266.
SMRi P32266. Positions 205-424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34606. 91 interactions.
DIPi DIP-8008N.
IntActi P32266. 6 interactions.
MINTi MINT-1354456.
STRINGi 4932.YOR211C.

Protein family/group databases

TCDBi 9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

Proteomic databases

MaxQBi P32266.
PaxDbi P32266.
PeptideAtlasi P32266.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR211C ; YOR211C ; YOR211C .
GeneIDi 854386.
KEGGi sce:YOR211C.

Organism-specific databases

SGDi S000005737. MGM1.

Phylogenomic databases

eggNOGi COG0699.
GeneTreei ENSGT00550000074851.
HOGENOMi HOG000206760.
OMAi YVGVVCK.
OrthoDBi EOG7ZD23R.

Enzyme and pathway databases

BioCyci YEAST:G3O-33713-MONOMER.

Miscellaneous databases

NextBioi 976535.
PMAP-CutDB P32266.

Gene expression databases

Genevestigatori P32266.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11566. PTHR11566. 1 hit.
Pfami PF00350. Dynamin_N. 1 hit.
[Graphical view ]
PRINTSi PR00195. DYNAMIN.
SMARTi SM00053. DYNc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin."
    Jones B.A., Fangman W.L.
    Genes Dev. 6:380-389(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BJ41-8C.
  2. "Normal mitochondrial structure and genome maintenance in yeast requires the dynamin-like product of the MGM1 gene."
    Guan K., Farh L., Marshall T., Deschenes R.J.
    Curr. Genet. 24:141-148(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
    Galisson F., Dujon B.
    Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-784.
    Strain: ATCC 96604 / S288c / FY1679.
  6. "Interactions between three common subunits of yeast RNA polymerases I and III."
    Lalo D., Carles C., Sentenac A., Thuriaux P.
    Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 713-881.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  7. "Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA."
    Herlan M., Vogel F., Bornhoevd C., Neupert W., Reichert A.S.
    J. Biol. Chem. 278:27781-27788(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 60-67 AND 140-147, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
    Strain: ATCC 200060 / W303.
  8. "The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance."
    Shepard K.A., Yaffe M.P.
    J. Cell Biol. 144:711-720(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF SER-224 AND GLU-294, IDENTIFICATION OF THE TRANSLATION INITIATION CODON.
  9. "The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion."
    Wong E.D., Wagner J.A., Scott S.V., Okreglak V., Holewinske T.J., Cassidy-Stone A., Nunnari J.
    J. Cell Biol. 160:303-311(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-223; SER-224; THR-244; ARG-824 AND LYS-854.
  10. "Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane."
    Sesaki H., Southard S.M., Yaffe M.P., Jensen R.E.
    Mol. Biol. Cell 14:2342-2356(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FZO1 AND UGO1.
  11. "Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion."
    Sesaki H., Jensen R.E.
    J. Biol. Chem. 279:28298-28303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UGO1.
  12. "Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor."
    Herlan M., Bornhoevd C., Hell K., Neupert W., Reichert A.S.
    J. Cell Biol. 165:167-173(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-79.
  13. "Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
    Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
    Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiMGM1_YEAST
AccessioniPrimary (citable) accession number: P32266
Secondary accession number(s): D6W2R7, Q02609, Q08627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 26, 2004
Last modified: July 9, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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