Reviewed, UniProtKB/Swiss-Prot P32266 (MGM1_YEAST)

Names and origin

Protein names

Recommended name:
    Dynamin-like GTPase MGM1, mitochondrial
Alternative name(s):
    Mitochondrial genome maintenance protein 1
    Mitochondrial division and morphology protein 17
Cleaved into the following 2 chains:
    1- Recommended name:
            Dynamin-like GTPase MGM1 large isoform
                Short name=l-MGM1
    2- Recommended name:
            Dynamin-like GTPase MGM1 small isoform
                Short name=s-MGM1

Gene names

Name:	MGM1
Synonyms:	MDM17
Ordered Locus Names:	YOR211C
ORF Names:	YOR50-1

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane. Ref.6 Ref.7 Ref.8 Ref.10
Subunit structure	Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain. Ref.7 Ref.8 Ref.12
Subcellular location	Dynamin-like GTPase MGM1 large isoform: Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side. Dynamin-like GTPase MGM1 small isoform: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Post-translational modification	Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.
Miscellaneous	Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to loose their mitochondrial DNA and become respiration deficient.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain.
Sequence caution	The sequence CAA44637.1 differs from that shown. Reason: Frameshift at position 4.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Signal-anchor Transit peptide Transmembrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial fusion Ref.8 Ref.9 Inferred from direct assay. Source: UniProtKB mitochondrial genome maintenance Ref.1 Ref.6 Inferred from direct assay. Source: UniProtKB mitochondrial membrane organization Ref.8 Inferred from direct assay. Source: UniProtKB mitochondrion inheritance Ref.6 Inferred from direct assay. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial intermembrane space Ref.6 Inferred from direct assay. Source: UniProtKB
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: SGD protein binding Ref.9 Ref.10 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q02895	1	EBI-10865,EBI-35030
CEM1	P39525	1	EBI-10865,EBI-4467
COX7	P10174	1	EBI-10865,EBI-2050505
FOX2	Q02207	1	EBI-10865,EBI-2060458
FPS1	P23900	1	EBI-10865,EBI-7074
FZO1	P38297	2	EBI-10865,EBI-20900
GCV1	P48015	1	EBI-10865,EBI-7499
HIS2	P38635	1	EBI-10865,EBI-2045554
ITC1	P53125	1	EBI-10865,EBI-23967
MCA1	Q08601	1	EBI-10865,EBI-30428
MLH2	Q07980	1	EBI-10865,EBI-33369
MND1	P53102	1	EBI-10865,EBI-24091
MSH3	P25336	1	EBI-10865,EBI-11362
NCB2	Q92317	1	EBI-10865,EBI-37723
NCR1	Q12200	1	EBI-10865,EBI-32032
NOP7	P53261	1	EBI-10865,EBI-13145
NSA2	P40078	1	EBI-10865,EBI-22681
ORC4	P54791	1	EBI-10865,EBI-12580
PGM1	P33401	1	EBI-10865,EBI-13289
PRM3	Q08931	1	EBI-10865,EBI-36479
RAD10	P06838	1	EBI-10865,EBI-14637
RFT1	P38206	1	EBI-10865,EBI-15028
RIB7	P33312	1	EBI-10865,EBI-15177
SGF29	P25554	1	EBI-10865,EBI-21678
SSE2	P32590	1	EBI-10865,EBI-8655
SSP120	P39931	1	EBI-10865,EBI-16366
TAF6	P53040	1	EBI-10865,EBI-18876
TIM13	P53299	1	EBI-10865,EBI-9121
TOM1	Q03280	1	EBI-10865,EBI-36817
TPC1	P53257	1	EBI-10865,EBI-2095999
UGO1	Q03327	2	EBI-10865,EBI-32955
UIP3	P39547	1	EBI-10865,EBI-20760
VPS73	P53142	1	EBI-10865,EBI-23899
YDC1	Q02896	1	EBI-10865,EBI-22203
YRF1-4	O13559	1	EBI-10865,EBI-29562

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Mitochondrion Ref.6
	Chain	60 – 881	822	Dynamin-like GTPase MGM1 large isoform		PRO_0000007400
	Chain	140 – 881	742	Dynamin-like GTPase MGM1 small isoform		PRO_0000007401
Regions
	Topological domain	60 – 72	13	Mitochondrial matrix Potential
	Transmembrane	73 – 92	20	Signal-anchor for type II membrane protein Potential
	Topological domain	93 – 881	789	Mitochondrial intermembrane Potential
	Domain	780 – 872	93	GED
	Nucleotide binding	217 – 224	8	GTP Potential
	Nucleotide binding	317 – 321	5	GTP Potential
	Nucleotide binding	385 – 388	4	GTP Potential
	Compositional bias	149 – 178	30	Asp-rich (acidic)
Experimental info
	Mutagenesis	1	1	M → A: Abolishes translation.
	Mutagenesis	39	1	M → A: No effect on translation.
	Mutagenesis	79	1	G → D or K: Loss of function; most MGM1 processed to s-MGM1. Ref.11
	Mutagenesis	81	1	M → A: No effect on translation.
	Mutagenesis	92	1	M → A: No effect on translation.
	Mutagenesis	223	1	K → A: Loss of stability. Ref.8
	Mutagenesis	224	1	S → A: Loss of GTPase activity. Ref.7 Ref.8
	Mutagenesis	224	1	S → N: Loss of GTPase activity. Ref.7 Ref.8
	Mutagenesis	244	1	T → A: Loss of GTPase activity. Ref.8
	Mutagenesis	294	1	E → K in mdm17; loss of function at 37 degrees Celsius. Ref.7
	Mutagenesis	824	1	R → A: Loss of GED function. Ref.8
	Mutagenesis	854	1	K → A: Loss of GED function. Ref.8
	Sequence conflict	4	1	S → T in CAA44637. Ref.1
	Sequence conflict	129	1	G → C in CAA44637. Ref.1
	Sequence conflict	170	1	E → A in L07419. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32266-1 [UniParc]. Last modified April 26, 2004. Version 3. Checksum: 31524B40C5350096	FASTA	881	99,178
	10 20 30 40 50 60 MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA LSFVITRRSI 70 80 90 100 110 120 SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK DKLDRIKDLG ESMKEKFNKM 130 140 150 160 170 180 FSGDKSQDGG HGNDGTVPTA TLIAATSLDD DESKRQGDPK DDDDEDDDDE DDENDSVDTT 190 200 210 220 230 240 QDEMLNLTKQ MIEIRTILNK VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS 250 260 270 280 290 300 NMVTRRPIEL TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP 310 320 330 340 350 360 IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI ILAISAADVD 370 380 390 400 410 420 LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN NKKYPLSMGY VGVITKTPSS 430 440 450 460 470 480 INRKHLGLFG EAPSSSLSGI FSKGQHGQSS GEENTNGLKQ IVSHQFEKAY FKENKKYFTN 490 500 510 520 530 540 CQVSTKKLRE KLIKILEISM SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN 550 560 570 580 590 600 IDVLKLGIKE FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM 610 620 630 640 650 660 LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI KDLVSNTAIN 670 680 690 700 710 720 VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN LIKEELRQCN SRYQAIKNAV 730 740 750 760 770 780 GSKKLANVMG YLENESNLQK ETLGMSKLLL ERGSEAIFLD KRCKVLSFRL KMLKNKCHST 790 800 810 820 830 840 IEKDRCPEVF LSAVSDKLTS TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE 850 860 870 880 DPKISRHIEL QKRKELLELA LEKIDSILVF KKSYKGVSKN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin." Jones B.A., Fangman W.L. Genes Dev. 6:380-389(1992) [PubMed: 1532158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BJ41-8C.
[2]	"Normal mitochondrial structure and genome maintenance in yeast requires the dynamin-like product of the MGM1 gene." Guan K., Farh L., Marshall T., Deschenes R.J. Curr. Genet. 24:141-148(1993) [PubMed: 7916673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae." Galisson F., Dujon B. Yeast 12:877-885(1996) [PubMed: 8840505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-784. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Interactions between three common subunits of yeast RNA polymerases I and III." Lalo D., Carles C., Sentenac A., Thuriaux P. Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed: 8516295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 713-881. Strain: ATCC 28383 / FL100 / VTT C-80102.
[6]	"Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA." Herlan M., Vogel F., Bornhoevd C., Neupert W., Reichert A.S. J. Biol. Chem. 278:27781-27788(2003) [PubMed: 12707284] [Abstract] Cited for: PROTEIN SEQUENCE OF 60-67 AND 140-147, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY. Strain: ATCC 200060 / W303.
[7]	"The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance." Shepard K.A., Yaffe M.P. J. Cell Biol. 144:711-720(1999) [PubMed: 10037792] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF SER-224 AND GLU-294, IDENTIFICATION OF THE TRANSLATION INITIATION CODON.
[8]	"The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion." Wong E.D., Wagner J.A., Scott S.V., Okreglak V., Holewinske T.J., Cassidy-Stone A., Nunnari J. J. Cell Biol. 160:303-311(2003) [PubMed: 12566426] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-223; SER-224; THR-244; ARG-824 AND LYS-854.
[9]	"Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane." Sesaki H., Southard S.M., Yaffe M.P., Jensen R.E. Mol. Biol. Cell 14:2342-2356(2003) [PubMed: 12808034] [Abstract] Cited for: INTERACTION WITH FZO1 AND UGO1.
[10]	"Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion." Sesaki H., Jensen R.E. J. Biol. Chem. 279:28298-28303(2004) [PubMed: 15087460] [Abstract] Cited for: FUNCTION, INTERACTION WITH UGO1.
[11]	"Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor." Herlan M., Bornhoevd C., Hell K., Neupert W., Reichert A.S. J. Cell Biol. 165:167-173(2004) [PubMed: 15096522] [Abstract] Cited for: PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-79.
[12]	"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins." Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C. Mol. Biol. Cell 17:1436-1450(2006) [PubMed: 16407407] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62834 Genomic DNA. Translation: CAA44637.1. Frameshift. L07419 Genomic DNA. No translation available. Z75119 Genomic DNA. Translation: CAA99426.1. Different initiation. Z75120 Genomic DNA. Translation: CAA99428.1. Different initiation. X92441 Genomic DNA. Translation: CAA63174.1. Different initiation. L11274 Genomic DNA. Translation: AAB59316.1.
PIR	S33918.
RefSeq	NP_014854.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:8008N.
IntAct	P32266. 86 interactions.
STRING	P32266.
Proteomic databases
PeptideAtlas	P32266.
Genome annotation databases
Ensembl	YOR211C; YOR211C; YOR211C; Saccharomyces cerevisiae. [Genome view]
GeneID	854386.
KEGG	sce:YOR211C.
NMPDR	fig|4932.3.peg.5966.
Organism-specific databases
CYGD	YOR211c.
SGD	S000005737. MGM1.
Phylogenomic databases
HOGENOM	P32266.
OrthoDB	EOG97H76F
Gene expression databases
ArrayExpress	P32266.
Genevestigator	P32266.
GermOnline	YOR211C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR020850. GTPase_effector_domain_GED. [Graphical view]
Pfam	PF00350. Dynamin_N. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976535.
PMAP-CutDB	P32266.

Entry information

Entry name

MGM1_YEAST

Accession

Primary (citable) accession number: P32266
Secondary accession number(s): Q02609, Q08627

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	April 26, 2004
Last modified:	November 24, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)