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Protein

Glutamate 5-kinase

Gene

PRO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline.

Miscellaneous

Present with 10400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (PRO1)
  2. Gamma-glutamyl phosphate reductase (PRO2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateBy similarity1
Binding sitei143SubstrateBy similarity1
Binding sitei155Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 176ATPBy similarity2
Nucleotide bindingi219 – 225ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate 5-kinase activity Source: SGD
  • identical protein binding Source: IntAct
  • RNA binding Source: InterPro

GO - Biological processi

  • L-proline biosynthetic process Source: UniProtKB-UniPathway
  • proline biosynthetic process Source: SGD
  • ribophagy Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YDR300C-MONOMER.
BRENDAi2.7.2.11. 984.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gene namesi
Name:PRO1
Ordered Locus Names:YDR300C
ORF Names:D9740.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR300C.
SGDiS000002708. PRO1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097681 – 428Glutamate 5-kinaseAdd BLAST428

Proteomic databases

MaxQBiP32264.
PRIDEiP32264.
TopDownProteomicsiP32264.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi32352. 29 interactors.
DIPiDIP-5221N.
IntActiP32264. 10 interactors.
MINTiMINT-516807.

Structurei

3D structure databases

ProteinModelPortaliP32264.
SMRiP32264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini321 – 412PUAAdd BLAST92

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075661.
HOGENOMiHOG000246369.
InParanoidiP32264.
KOiK00931.
OMAiREPRIAN.
OrthoDBiEOG092C2KL8.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

P32264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDANESKSY TIVIKLGSSS LVDEKTKEPK LAIMSLIVET VVKLRRMGHK
60 70 80 90 100
VIIVSSGGIA VGLRTMRMNK RPKHLAEVQA IAAIGQGRLI GRWDLLFSQF
110 120 130 140 150
DQRIAQILLT RNDILDWTQY KNAQNTINEL LNMGVIPIVN ENDTLSVREI
160 170 180 190 200
KFGDNDTLSA ITSALIHADY LFLLTDVDCL YTDNPRTNPD AMPILVVPDL
210 220 230 240 250
SKGLPGVNTA GGSGSDVGTG GMETKLVAAD LATNAGVHTL IMKSDTPANI
260 270 280 290 300
GRIVEYMQTL ELDDENKVKQ AYNGDLTDLQ KREFEKLKAL NVPLHTKFIA
310 320 330 340 350
NDNKHHLKNR EFWILHGLVS KGAVVIDQGA YAALTRKNKA GLLPAGVIDV
360 370 380 390 400
QGTFHELECV DIKVGKKLPD GTLDPDFPLQ TVGKARCNYT SSELTKIKGL
410 420
HSDQIEEELG YNDSEYVAHR ENLAFPPR
Length:428
Mass (Da):47,162
Last modified:October 1, 1996 - v2
Checksum:i0DBAC7CA98051C80
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti332A → R in AAA34904 (PubMed:1350780).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85293 Genomic DNA. Translation: AAA34904.1.
U28374 Genomic DNA. Translation: AAB64736.1.
BK006938 Genomic DNA. Translation: DAA12139.1.
PIRiS61186.
RefSeqiNP_010586.3. NM_001180608.3.

Genome annotation databases

EnsemblFungiiYDR300C; YDR300C; YDR300C.
GeneIDi851894.
KEGGisce:YDR300C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85293 Genomic DNA. Translation: AAA34904.1.
U28374 Genomic DNA. Translation: AAB64736.1.
BK006938 Genomic DNA. Translation: DAA12139.1.
PIRiS61186.
RefSeqiNP_010586.3. NM_001180608.3.

3D structure databases

ProteinModelPortaliP32264.
SMRiP32264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32352. 29 interactors.
DIPiDIP-5221N.
IntActiP32264. 10 interactors.
MINTiMINT-516807.

Proteomic databases

MaxQBiP32264.
PRIDEiP32264.
TopDownProteomicsiP32264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR300C; YDR300C; YDR300C.
GeneIDi851894.
KEGGisce:YDR300C.

Organism-specific databases

EuPathDBiFungiDB:YDR300C.
SGDiS000002708. PRO1.

Phylogenomic databases

GeneTreeiENSGT00550000075661.
HOGENOMiHOG000246369.
InParanoidiP32264.
KOiK00931.
OMAiREPRIAN.
OrthoDBiEOG092C2KL8.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciYEAST:YDR300C-MONOMER.
BRENDAi2.7.2.11. 984.

Miscellaneous databases

PROiPR:P32264.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_YEAST
AccessioniPrimary (citable) accession number: P32264
Secondary accession number(s): D6VSS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: March 15, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.