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P32264

- PROB_YEAST

UniProt

P32264 - PROB_YEAST

Protein

Glutamate 5-kinase

Gene

PRO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline.

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561SubstrateBy similarity
    Binding sitei143 – 1431SubstrateBy similarity
    Binding sitei155 – 1551Substrate; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi175 – 1762ATPBy similarity
    Nucleotide bindingi219 – 2257ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: SGD
    3. identical protein binding Source: IntAct
    4. protein binding Source: IntAct
    5. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway
    2. proline biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YDR300C-MONOMER.
    BRENDAi2.7.2.11. 984.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinase (EC:2.7.2.11)
    Short name:
    GK
    Alternative name(s):
    Gamma-glutamyl kinase
    Gene namesi
    Name:PRO1
    Ordered Locus Names:YDR300C
    ORF Names:D9740.12
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR300c.
    SGDiS000002708. PRO1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Glutamate 5-kinasePRO_0000109768Add
    BLAST

    Proteomic databases

    MaxQBiP32264.
    PaxDbiP32264.
    PeptideAtlasiP32264.
    PRIDEiP32264.

    Expressioni

    Gene expression databases

    GenevestigatoriP32264.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-13879,EBI-13879
    HSP82P028293EBI-13879,EBI-8659

    Protein-protein interaction databases

    BioGridi32352. 26 interactions.
    DIPiDIP-5221N.
    IntActiP32264. 10 interactions.
    MINTiMINT-516807.
    STRINGi4932.YDR300C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32264.
    SMRiP32264. Positions 9-423.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini321 – 41292PUAAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.Curated
    Contains 1 PUA domain.Curated

    Phylogenomic databases

    eggNOGiCOG0263.
    GeneTreeiENSGT00550000075661.
    HOGENOMiHOG000246369.
    KOiK00931.
    OMAiPRIENAR.
    OrthoDBiEOG7SFJ62.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32264-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDANESKSY TIVIKLGSSS LVDEKTKEPK LAIMSLIVET VVKLRRMGHK    50
    VIIVSSGGIA VGLRTMRMNK RPKHLAEVQA IAAIGQGRLI GRWDLLFSQF 100
    DQRIAQILLT RNDILDWTQY KNAQNTINEL LNMGVIPIVN ENDTLSVREI 150
    KFGDNDTLSA ITSALIHADY LFLLTDVDCL YTDNPRTNPD AMPILVVPDL 200
    SKGLPGVNTA GGSGSDVGTG GMETKLVAAD LATNAGVHTL IMKSDTPANI 250
    GRIVEYMQTL ELDDENKVKQ AYNGDLTDLQ KREFEKLKAL NVPLHTKFIA 300
    NDNKHHLKNR EFWILHGLVS KGAVVIDQGA YAALTRKNKA GLLPAGVIDV 350
    QGTFHELECV DIKVGKKLPD GTLDPDFPLQ TVGKARCNYT SSELTKIKGL 400
    HSDQIEEELG YNDSEYVAHR ENLAFPPR 428
    Length:428
    Mass (Da):47,162
    Last modified:October 1, 1996 - v2
    Checksum:i0DBAC7CA98051C80
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti332 – 3321A → R in AAA34904. (PubMed:1350780)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85293 Genomic DNA. Translation: AAA34904.1.
    U28374 Genomic DNA. Translation: AAB64736.1.
    BK006938 Genomic DNA. Translation: DAA12139.1.
    PIRiS61186.
    RefSeqiNP_010586.3. NM_001180608.3.

    Genome annotation databases

    EnsemblFungiiYDR300C; YDR300C; YDR300C.
    GeneIDi851894.
    KEGGisce:YDR300C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85293 Genomic DNA. Translation: AAA34904.1 .
    U28374 Genomic DNA. Translation: AAB64736.1 .
    BK006938 Genomic DNA. Translation: DAA12139.1 .
    PIRi S61186.
    RefSeqi NP_010586.3. NM_001180608.3.

    3D structure databases

    ProteinModelPortali P32264.
    SMRi P32264. Positions 9-423.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32352. 26 interactions.
    DIPi DIP-5221N.
    IntActi P32264. 10 interactions.
    MINTi MINT-516807.
    STRINGi 4932.YDR300C.

    Proteomic databases

    MaxQBi P32264.
    PaxDbi P32264.
    PeptideAtlasi P32264.
    PRIDEi P32264.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR300C ; YDR300C ; YDR300C .
    GeneIDi 851894.
    KEGGi sce:YDR300C.

    Organism-specific databases

    CYGDi YDR300c.
    SGDi S000002708. PRO1.

    Phylogenomic databases

    eggNOGi COG0263.
    GeneTreei ENSGT00550000075661.
    HOGENOMi HOG000246369.
    KOi K00931.
    OMAi PRIENAR.
    OrthoDBi EOG7SFJ62.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci YEAST:YDR300C-MONOMER.
    BRENDAi 2.7.2.11. 984.

    Miscellaneous databases

    NextBioi 969886.

    Gene expression databases

    Genevestigatori P32264.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Proline biosynthesis in Saccharomyces cerevisiae: molecular analysis of the PRO1 gene, which encodes gamma-glutamyl kinase."
      Li W., Brandriss M.C.
      J. Bacteriol. 174:4148-4156(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPROB_YEAST
    AccessioniPrimary (citable) accession number: P32264
    Secondary accession number(s): D6VSS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10400 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3