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Protein

Pyrroline-5-carboxylate reductase

Gene

PRO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 43500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrroline-5-carboxylate reductase (PRO3)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • pyrroline-5-carboxylate reductase activity Source: SGD

GO - Biological processi

  • L-proline biosynthetic process Source: UniProtKB-UniPathway
  • proline biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:YER023W-MONOMER
YEAST:YER023W-MONOMER
ReactomeiR-SCE-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00098; UER00361

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase (EC:1.5.1.2)
Short name:
P5C reductase
Short name:
P5CR
Gene namesi
Name:PRO3
Synonyms:ORE2
Ordered Locus Names:YER023W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER023W
SGDiS000000825 PRO3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001873281 – 286Pyrroline-5-carboxylate reductaseAdd BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki171Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei246PhosphothreonineCombined sources1
Modified residuei279PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32263
PaxDbiP32263
PRIDEiP32263

PTM databases

iPTMnetiP32263

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-13885,EBI-13885

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi36757, 35 interactors
DIPiDIP-1529N
IntActiP32263, 10 interactors
MINTiP32263
STRINGi4932.YER023W

Structurei

3D structure databases

ProteinModelPortaliP32263
SMRiP32263
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000007443
HOGENOMiHOG000230247
InParanoidiP32263
KOiK00286
OMAiRVMTNTP
OrthoDBiEOG092C39UX

Family and domain databases

HAMAPiMF_01925 P5C_reductase, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR036291 NAD(P)-bd_dom_sf
IPR028939 P5C_Rdtase_cat_N
IPR029036 P5CR_dimer
IPR000304 Pyrroline-COOH_reductase
PfamiView protein in Pfam
PF03807 F420_oxidored, 1 hit
PF14748 P5CR_dimer, 1 hit
PIRSFiPIRSF000193 Pyrrol-5-carb_rd, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00112 proC, 1 hit
PROSITEiView protein in PROSITE
PS00521 P5CR, 1 hit

Sequencei

Sequence statusi: Complete.

P32263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYTLAILGC GVMGQALLSA IYNAPKAADE TAAAFYPSKI ITCNHDEPSA
60 70 80 90 100
QQVTDLVETF DESPNGIKVE STYGHNVSAV EEASVVLLGT KPFLAEEVLN
110 120 130 140 150
GVKSVIGGKL LISLAAGWTI DQLSQYTSTV CRVMTNTPAK YGYGCAVVSY
160 170 180 190 200
SADVSKEQKP LVNELISQVG KYVELPEKNM DAATALVGSG PAFVLLMLES
210 220 230 240 250
LMESGLKLGI PLQESKECAM KVLEGTVKMV EKSGAHPSVL KHQVCTPGGT
260 270 280
TIAGLCVMEE KGVKSGIING VEEAARVASQ LGQKKK
Length:286
Mass (Da):30,132
Last modified:October 1, 1993 - v1
Checksum:iAEB71D93B46D08B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57886 Genomic DNA Translation: AAA34905.1
X57338 Genomic DNA Translation: CAA40614.1
U18778 Genomic DNA Translation: AAB64556.1
BK006939 Genomic DNA Translation: DAA07676.1
PIRiS25293
RefSeqiNP_010940.3, NM_001178914.3

Genome annotation databases

EnsemblFungiiYER023W; YER023W; YER023W
GeneIDi856744
KEGGisce:YER023W

Similar proteinsi

Entry informationi

Entry nameiP5CR_YEAST
AccessioniPrimary (citable) accession number: P32263
Secondary accession number(s): D3DLS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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