Reviewed, UniProtKB/Swiss-Prot P32263 (P5CR_YEAST)

Names and origin

Protein names

Recommended name:
    Pyrroline-5-carboxylate reductase
      Short name=P5C reductase
      Short name=P5CR
    EC=1.5.1.2

Gene names

Name:	PRO3
Synonyms:	ORE2
Ordered Locus Names:	YER023W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subunit structure	Homotetramer.
Miscellaneous	Present with 43500 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct pyrroline-5-carboxylate reductase activity Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-13885,EBI-13885
ARO8	P53090	1	EBI-13885,EBI-2042933
ATX1	P38636	1	EBI-13885,EBI-2046026
CAR2	P07991	1	EBI-13885,EBI-12430
CYS4	P32582	1	EBI-13885,EBI-4167
GLN1	P32288	1	EBI-13885,EBI-7665
IPP1	P00817	1	EBI-13885,EBI-9338
MEX67	Q99257	1	EBI-13885,EBI-11642
OYE2	Q03558	1	EBI-13885,EBI-12729
SEC27	P41811	1	EBI-13885,EBI-4898
TAF2	P23255	1	EBI-13885,EBI-18862
UBA1	P22515	1	EBI-13885,EBI-19703
VAS1	P07806-1	1	EBI-13885,EBI-1009942
VMA4	P22203	1	EBI-13885,EBI-20268

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Pyrroline-5-carboxylate reductase		PRO_0000187328
Amino acid modifications
	Modified residue	246	1	Phosphothreonine Ref.6
	Modified residue	279	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P32263-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: AEB71D93B46D08B3	FASTA	286	30,132
	10 20 30 40 50 60 MTYTLAILGC GVMGQALLSA IYNAPKAADE TAAAFYPSKI ITCNHDEPSA QQVTDLVETF 70 80 90 100 110 120 DESPNGIKVE STYGHNVSAV EEASVVLLGT KPFLAEEVLN GVKSVIGGKL LISLAAGWTI 130 140 150 160 170 180 DQLSQYTSTV CRVMTNTPAK YGYGCAVVSY SADVSKEQKP LVNELISQVG KYVELPEKNM 190 200 210 220 230 240 DAATALVGSG PAFVLLMLES LMESGLKLGI PLQESKECAM KVLEGTVKMV EKSGAHPSVL 250 260 270 280 KHQVCTPGGT TIAGLCVMEE KGVKSGIING VEEAARVASQ LGQKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proline biosynthesis in Saccharomyces cerevisiae: analysis of the PRO3 gene, which encodes delta 1-pyrroline-5-carboxylate reductase." Brandriss M.C., Falvey D.A. J. Bacteriol. 174:3782-3788(1992) [PubMed: 1592829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Brandriss M.C., Falvey D.A. J. Bacteriol. 174:5176-5176(1992) [PubMed: 1352771] [Abstract]
[3]	"ore2, a mutation affecting proline biosynthesis in the yeast Saccharomyces cerevisiae, leads to a cdc phenotype." Neuville P., Aigle M. Mol. Gen. Genet. 234:193-200(1992) [PubMed: 1508147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-246 AND SER-279, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M57886 Genomic DNA. Translation: AAA34905.1. X57338 Genomic DNA. Translation: CAA40614.1. U18778 Genomic DNA. Translation: AAB64556.1.
PIR	S25293.
RefSeq	NP_010940.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1529N.
IntAct	P32263. 25 interactions.
Proteomic databases
PeptideAtlas	P32263.
PRIDE	P32263.
Genome annotation databases
Ensembl	YER023W. Saccharomyces cerevisiae. [Contig view]
GeneID	856744.
GenomeReviews	Gene locus YER023W in contig U00092_GR.
KEGG	sce:YER023W.
NMPDR	fig|4932.3.peg.2000.
Organism-specific databases
CYGD	YER023w.
SGD	S000000825. PRO3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P32263.
OMA	P32263. APSGHSK.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11544.
BRENDA	1.5.1.2. 250.
Gene expression databases
GermOnline	YER023W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004455. NADP_OxRdtase_F420. IPR000304. P5CR. [Graphical view]
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982882.

Entry information

Entry name

P5CR_YEAST

Accession

Primary (citable) accession number: P32263

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)