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Reviewed, UniProtKB/Swiss-Prot P32263 (P5CR_YEAST)

Last modified June 16, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Pyrroline-5-carboxylate reductase
      Short name=P5C reductase
      Short name=P5CR
    EC=1.5.1.2
Gene names
Name: PRO3
Synonyms: ORE2
Ordered Locus Names: YER023W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.

Subunit structure

Homotetramer.

Miscellaneous

Present with 43500 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Pyrroline-5-carboxylate reductase
PRO_0000187328

Amino acid modifications

Modified residue2461Phosphothreonine Ref.6
Modified residue2791Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P32263-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: AEB71D93B46D08B3

FASTA28630,132
        10         20         30         40         50         60 
MTYTLAILGC GVMGQALLSA IYNAPKAADE TAAAFYPSKI ITCNHDEPSA QQVTDLVETF 

        70         80         90        100        110        120 
DESPNGIKVE STYGHNVSAV EEASVVLLGT KPFLAEEVLN GVKSVIGGKL LISLAAGWTI 

       130        140        150        160        170        180 
DQLSQYTSTV CRVMTNTPAK YGYGCAVVSY SADVSKEQKP LVNELISQVG KYVELPEKNM 

       190        200        210        220        230        240 
DAATALVGSG PAFVLLMLES LMESGLKLGI PLQESKECAM KVLEGTVKMV EKSGAHPSVL 

       250        260        270        280 
KHQVCTPGGT TIAGLCVMEE KGVKSGIING VEEAARVASQ LGQKKK 

« Hide

References

« Hide 'large scale' references
[1]"Proline biosynthesis in Saccharomyces cerevisiae: analysis of the PRO3 gene, which encodes delta 1-pyrroline-5-carboxylate reductase."
Brandriss M.C., Falvey D.A.
J. Bacteriol. 174:3782-3788(1992) [PubMed: 1592829] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Erratum
Brandriss M.C., Falvey D.A.
J. Bacteriol. 174:5176-5176(1992) [PubMed: 1352771] [Abstract]
[3]"ore2, a mutation affecting proline biosynthesis in the yeast Saccharomyces cerevisiae, leads to a cdc phenotype."
Neuville P., Aigle M.
Mol. Gen. Genet. 234:193-200(1992) [PubMed: 1508147] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-246 AND SER-279, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

M57886 Genomic DNA. Translation: AAA34905.1.
X57338 Genomic DNA. Translation: CAA40614.1.
U18778 Genomic DNA. Translation: AAB64556.1.
PIRS25293.
RefSeqNP_010940.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1529N.
IntActP32263. 25 interactions.

Proteomic databases

PeptideAtlasP32263.
PRIDEP32263.

Genome annotation databases

EnsemblYER023W. Saccharomyces cerevisiae. [Contig view]
GeneID856744.
GenomeReviewsGene locus YER023W in contig U00092_GR.
KEGGsce:YER023W.
NMPDRfig|4932.3.peg.2000.

Organism-specific databases

CYGDYER023w.
SGDS000000825. PRO3.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP32263.
OMAP32263. APSGHSK.

Enzyme and pathway databases

BioCycMetaCyc:MON-11544.
BRENDA1.5.1.2. 250.

Gene expression databases

GermOnlineYER023W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004455. NADP_OxRdtase_F420.
IPR000304. P5CR.
[Graphical view]
PANTHERPTHR11645. P5CR. 1 hit.
PfamPF03807. F420_oxidored. 1 hit.
[Graphical view]
PIRSFPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
TIGRFAMsTIGR00112. proC. 1 hit.
PROSITEPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio982882.

Entry information

Entry nameP5CR_YEAST
AccessionPrimary (citable) accession number: P32263
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents