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P32263

- P5CR_YEAST

UniProt

P32263 - P5CR_YEAST

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Protein

Pyrroline-5-carboxylate reductase

Gene

PRO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathwayi

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. pyrroline-5-carboxylate reductase activity Source: SGD

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
  2. proline biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11544.
YEAST:YER023W-MONOMER.
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase (EC:1.5.1.2)
Short name:
P5C reductase
Short name:
P5CR
Gene namesi
Name:PRO3
Synonyms:ORE2
Ordered Locus Names:YER023W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER023w.
SGDiS000000825. PRO3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Pyrroline-5-carboxylate reductasePRO_0000187328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei246 – 2461Phosphothreonine1 Publication
Modified residuei279 – 2791Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32263.
PaxDbiP32263.
PeptideAtlasiP32263.

Expressioni

Gene expression databases

GenevestigatoriP32263.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-13885,EBI-13885

Protein-protein interaction databases

BioGridi36757. 34 interactions.
DIPiDIP-1529N.
IntActiP32263. 11 interactions.
MINTiMINT-411759.
STRINGi4932.YER023W.

Structurei

3D structure databases

ProteinModelPortaliP32263.
SMRiP32263. Positions 5-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0345.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
InParanoidiP32263.
KOiK00286.
OMAiCCKPQQA.
OrthoDBiEOG76DV3T.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32263-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTYTLAILGC GVMGQALLSA IYNAPKAADE TAAAFYPSKI ITCNHDEPSA
60 70 80 90 100
QQVTDLVETF DESPNGIKVE STYGHNVSAV EEASVVLLGT KPFLAEEVLN
110 120 130 140 150
GVKSVIGGKL LISLAAGWTI DQLSQYTSTV CRVMTNTPAK YGYGCAVVSY
160 170 180 190 200
SADVSKEQKP LVNELISQVG KYVELPEKNM DAATALVGSG PAFVLLMLES
210 220 230 240 250
LMESGLKLGI PLQESKECAM KVLEGTVKMV EKSGAHPSVL KHQVCTPGGT
260 270 280
TIAGLCVMEE KGVKSGIING VEEAARVASQ LGQKKK
Length:286
Mass (Da):30,132
Last modified:October 1, 1993 - v1
Checksum:iAEB71D93B46D08B3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57886 Genomic DNA. Translation: AAA34905.1.
X57338 Genomic DNA. Translation: CAA40614.1.
U18778 Genomic DNA. Translation: AAB64556.1.
BK006939 Genomic DNA. Translation: DAA07676.1.
PIRiS25293.
RefSeqiNP_010940.3. NM_001178914.3.

Genome annotation databases

EnsemblFungiiYER023W; YER023W; YER023W.
GeneIDi856744.
KEGGisce:YER023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57886 Genomic DNA. Translation: AAA34905.1 .
X57338 Genomic DNA. Translation: CAA40614.1 .
U18778 Genomic DNA. Translation: AAB64556.1 .
BK006939 Genomic DNA. Translation: DAA07676.1 .
PIRi S25293.
RefSeqi NP_010940.3. NM_001178914.3.

3D structure databases

ProteinModelPortali P32263.
SMRi P32263. Positions 5-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36757. 34 interactions.
DIPi DIP-1529N.
IntActi P32263. 11 interactions.
MINTi MINT-411759.
STRINGi 4932.YER023W.

Proteomic databases

MaxQBi P32263.
PaxDbi P32263.
PeptideAtlasi P32263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER023W ; YER023W ; YER023W .
GeneIDi 856744.
KEGGi sce:YER023W.

Organism-specific databases

CYGDi YER023w.
SGDi S000000825. PRO3.

Phylogenomic databases

eggNOGi COG0345.
GeneTreei ENSGT00390000007443.
HOGENOMi HOG000230247.
InParanoidi P32263.
KOi K00286.
OMAi CCKPQQA.
OrthoDBi EOG76DV3T.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00361 .
BioCyci MetaCyc:MONOMER-11544.
YEAST:YER023W-MONOMER.

Miscellaneous databases

NextBioi 982882.
PROi P32263.

Gene expression databases

Genevestigatori P32263.

Family and domain databases

Gene3Di 1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01925. P5C_reductase.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view ]
PANTHERi PTHR11645. PTHR11645. 1 hit.
Pfami PF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view ]
PIRSFi PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR00112. proC. 1 hit.
PROSITEi PS00521. P5CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Proline biosynthesis in Saccharomyces cerevisiae: analysis of the PRO3 gene, which encodes delta 1-pyrroline-5-carboxylate reductase."
    Brandriss M.C., Falvey D.A.
    J. Bacteriol. 174:3782-3788(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erratum
    Brandriss M.C., Falvey D.A.
    J. Bacteriol. 174:5176-5176(1992) [PubMed] [Europe PMC] [Abstract]
  3. "ore2, a mutation affecting proline biosynthesis in the yeast Saccharomyces cerevisiae, leads to a cdc phenotype."
    Neuville P., Aigle M.
    Mol. Gen. Genet. 234:193-200(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-246 AND SER-279, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiP5CR_YEAST
AccessioniPrimary (citable) accession number: P32263
Secondary accession number(s): D3DLS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 43500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3