P32263 (P5CR_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrroline-5-carboxylate reductase
Short name=P5C reductase
Short name=P5CR
EC=1.5.1.2

Gene names

Name:	PRO3
Synonyms:	ORE2
Ordered Locus Names:	YER023W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subunit structure	Homotetramer.
Miscellaneous	Present with 43500 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway proline biosynthetic process Inferred from mutant phenotype Ref.3PubMed 2824433. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 7009582. Source: SGD
Molecular_function	pyrroline-5-carboxylate reductase activity Inferred from genetic interaction PubMed 2824433. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		4	EBI-13885,EBI-13885

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Pyrroline-5-carboxylate reductase		PRO_0000187328
Amino acid modifications
	Modified residue	246	1	Phosphothreonine Ref.7
	Modified residue	279	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P32263 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: AEB71D93B46D08B3	FASTA	286	30,132
	10 20 30 40 50 60 MTYTLAILGC GVMGQALLSA IYNAPKAADE TAAAFYPSKI ITCNHDEPSA QQVTDLVETF 70 80 90 100 110 120 DESPNGIKVE STYGHNVSAV EEASVVLLGT KPFLAEEVLN GVKSVIGGKL LISLAAGWTI 130 140 150 160 170 180 DQLSQYTSTV CRVMTNTPAK YGYGCAVVSY SADVSKEQKP LVNELISQVG KYVELPEKNM 190 200 210 220 230 240 DAATALVGSG PAFVLLMLES LMESGLKLGI PLQESKECAM KVLEGTVKMV EKSGAHPSVL 250 260 270 280 KHQVCTPGGT TIAGLCVMEE KGVKSGIING VEEAARVASQ LGQKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proline biosynthesis in Saccharomyces cerevisiae: analysis of the PRO3 gene, which encodes delta 1-pyrroline-5-carboxylate reductase." Brandriss M.C., Falvey D.A. J. Bacteriol. 174:3782-3788(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Brandriss M.C., Falvey D.A. J. Bacteriol. 174:5176-5176(1992) [PubMed] [Europe PMC] [Abstract]
[3]	"ore2, a mutation affecting proline biosynthesis in the yeast Saccharomyces cerevisiae, leads to a cdc phenotype." Neuville P., Aigle M. Mol. Gen. Genet. 234:193-200(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-246 AND SER-279, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57886 Genomic DNA. Translation: AAA34905.1. X57338 Genomic DNA. Translation: CAA40614.1. U18778 Genomic DNA. Translation: AAB64556.1. BK006939 Genomic DNA. Translation: DAA07676.1.
PIR	S25293.
RefSeq	NP_010940.3. NM_001178914.3.
3D structure databases
ProteinModelPortal	P32263.
SMR	P32263. Positions 5-282.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1529N.
IntAct	P32263. 9 interactions.
MINT	MINT-411759.
STRING	4932.YER023W.
Proteomic databases
PaxDb	P32263.
PeptideAtlas	P32263.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER023W; YER023W; YER023W.
GeneID	856744.
KEGG	sce:YER023W.
Organism-specific databases
CYGD	YER023w.
SGD	S000000825. PRO3.
Phylogenomic databases
eggNOG	COG0345.
GeneTree	ENSGT00390000007443.
HOGENOM	HOG000230247.
KO	K00286.
OMA	THPGAMK.
OrthoDB	EOG4GQTDW.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11544. YEAST:YER023W-MONOMER.
UniPathway	UPA00098; UER00361.
Gene expression databases
Genevestigator	P32263.
GermOnline	YER023W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. Pyrroline-COOH_reductase. [Graphical view]
PANTHER	PTHR11645. PTHR11645. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982882.

Entry information

Entry name

P5CR_YEAST

Accession

Primary (citable) accession number: P32263
Secondary accession number(s): D3DLS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 29, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program