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P32261 (ANT3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Antithrombin-III

Short name=ATIII
Alternative name(s):
Serpin C1
Gene names
Name:Serpinc1
Synonyms:At3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin By similarity.

Subunit structure

Forms protease inhibiting heterodimer with TMPRSS7 By similarity.

Subcellular location

Secretedextracellular space By similarity.

Tissue specificity

Plasma.

Post-translational modification

Phosphorylation sites are present in the extracellular medium By similarity.

Sequence similarities

Belongs to the serpin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 By similarity
Chain33 – 465433Antithrombin-III
PRO_0000032490

Sites

Binding site821Heparin By similarity
Binding site1621Heparin By similarity
Binding site1781Heparin By similarity
Site426 – 4272Reactive bond

Amino acid modifications

Glycosylation1291N-linked (GlcNAc...) Ref.4
Glycosylation1681N-linked (GlcNAc...) Ref.4
Glycosylation1881N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation2251N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 161 By similarity
Disulfide bond54 ↔ 128 By similarity
Disulfide bond280 ↔ 463 By similarity

Sequences

Sequence LengthMass (Da)Tools
P32261 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 5CE087E98874E35D

FASTA46552,004
        10         20         30         40         50         60 
MYSPGAGSGA AGERKLCLLS LLLIGALGCA ICHGNPVDDI CIAKPRDIPV NPLCIYRSPG 

        70         80         90        100        110        120 
KKATEEDGSE QKVPEATNRR VWELSKANSR FATNFYQHLA DSKNDNDNIF LSPLSISTAF 

       130        140        150        160        170        180 
AMTKLGACND TLKQLMEVFK FDTISEKTSD QIHFFFAKLN CRLYRKANKS SDLVSANRLF 

       190        200        210        220        230        240 
GDKSLTFNES YQDVSEVVYG AKLQPLDFKE NPEQSRVTIN NWVANKTEGR IKDVIPQGAI 

       250        260        270        280        290        300 
NELTALVLVN TIYFKGLWKS KFSPENTRKE PFYKVDGQSC PVPMMYQEGK FKYRRVAEGT 

       310        320        330        340        350        360 
QVLELPFKGD DITMVLILPK PEKSLAKVEQ ELTPELLQEW LDELSETMLV VHMPRFRTED 

       370        380        390        400        410        420 
GFSLKEQLQD MGLIDLFSPE KSQLPGIVAG GRDDLYVSDA FHKAFLEVNE EGSEAAASTS 

       430        440        450        460 
VVITGRSLNP NRVTFKANRP FLVLIREVAL NTIIFMGRVA NPCVN 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and cell-free expression of mouse antithrombin III."
Wu J.K., Sheffield W.P., Blajchman M.A.
Thromb. Haemost. 68:291-296(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[3]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-188.
Strain: C57BL/6.
Tissue: Plasma.
[4]"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
Bernhard O.K., Kapp E.A., Simpson R.J.
J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129; ASN-168 AND ASN-188.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S47225 mRNA. Translation: AAB23965.1.
BC019447 mRNA. Translation: AAH19447.1.
BC033377 mRNA. Translation: AAH33377.1.
CCDSCCDS15411.1.
RefSeqNP_543120.1. NM_080844.4.
XP_006496689.1. XM_006496626.1.
UniGeneMm.260770.

3D structure databases

ProteinModelPortalP32261.
SMRP32261. Positions 39-464.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP32261. 3 interactions.
MINTMINT-1846278.
STRING10090.ENSMUSP00000068971.

Protein family/group databases

MEROPSI04.018.

PTM databases

PhosphoSiteP32261.

Proteomic databases

MaxQBP32261.
PaxDbP32261.
PRIDEP32261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064725; ENSMUSP00000068971; ENSMUSG00000026715.
GeneID11905.
KEGGmmu:11905.
UCSCuc007dem.2. mouse.

Organism-specific databases

CTD462.
MGIMGI:88095. Serpinc1.

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG005957.
InParanoidP32261.
KOK03911.
OMAKADGESC.
OrthoDBEOG7327PB.
PhylomeDBP32261.
TreeFamTF343094.

Gene expression databases

BgeeP32261.
GenevestigatorP32261.

Family and domain databases

InterProIPR015555. AT-III.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PTHR11461:SF53. PTHR11461:SF53. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279953.
PROP32261.
SOURCESearch...

Entry information

Entry nameANT3_MOUSE
AccessionPrimary (citable) accession number: P32261
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot