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Protein

Antithrombin-III

Gene

Serpinc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821HeparinBy similarity
Binding sitei162 – 1621HeparinBy similarity
Binding sitei178 – 1781HeparinBy similarity
Sitei426 – 4272Reactive bond

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_286713. Common Pathway of Fibrin Clot Formation.
REACT_340184. Intrinsic Pathway of Fibrin Clot Formation.

Protein family/group databases

MEROPSiI04.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Antithrombin-III
Short name:
ATIII
Alternative name(s):
Serpin C1
Gene namesi
Name:Serpinc1
Synonyms:At3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88095. Serpinc1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232By similarityAdd
BLAST
Chaini33 – 465433Antithrombin-IIIPRO_0000032490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 161By similarity
Disulfide bondi54 ↔ 128By similarity
Modified residuei69 – 691PhosphoserineBy similarity
Glycosylationi129 – 1291N-linked (GlcNAc...)1 Publication
Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication
Glycosylationi188 – 1881N-linked (GlcNAc...)2 Publications
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi280 ↔ 463By similarity

Post-translational modificationi

Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32261.
PaxDbiP32261.
PRIDEiP32261.

PTM databases

PhosphoSiteiP32261.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiP32261.
ExpressionAtlasiP32261. baseline and differential.
GenevisibleiP32261. MM.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7.By similarity

Protein-protein interaction databases

IntActiP32261. 3 interactions.
MINTiMINT-1846278.
STRINGi10090.ENSMUSP00000068971.

Structurei

3D structure databases

ProteinModelPortaliP32261.
SMRiP32261. Positions 39-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP32261.
KOiK03911.
OMAiWGCVTCH.
OrthoDBiEOG7327PB.
PhylomeDBiP32261.
TreeFamiTF343094.

Family and domain databases

InterProiIPR015555. AT-III.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PTHR11461:SF53. PTHR11461:SF53. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSPGAGSGA AGERKLCLLS LLLIGALGCA ICHGNPVDDI CIAKPRDIPV
60 70 80 90 100
NPLCIYRSPG KKATEEDGSE QKVPEATNRR VWELSKANSR FATNFYQHLA
110 120 130 140 150
DSKNDNDNIF LSPLSISTAF AMTKLGACND TLKQLMEVFK FDTISEKTSD
160 170 180 190 200
QIHFFFAKLN CRLYRKANKS SDLVSANRLF GDKSLTFNES YQDVSEVVYG
210 220 230 240 250
AKLQPLDFKE NPEQSRVTIN NWVANKTEGR IKDVIPQGAI NELTALVLVN
260 270 280 290 300
TIYFKGLWKS KFSPENTRKE PFYKVDGQSC PVPMMYQEGK FKYRRVAEGT
310 320 330 340 350
QVLELPFKGD DITMVLILPK PEKSLAKVEQ ELTPELLQEW LDELSETMLV
360 370 380 390 400
VHMPRFRTED GFSLKEQLQD MGLIDLFSPE KSQLPGIVAG GRDDLYVSDA
410 420 430 440 450
FHKAFLEVNE EGSEAAASTS VVITGRSLNP NRVTFKANRP FLVLIREVAL
460
NTIIFMGRVA NPCVN
Length:465
Mass (Da):52,004
Last modified:October 1, 1993 - v1
Checksum:i5CE087E98874E35D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S47225 mRNA. Translation: AAB23965.1.
BC019447 mRNA. Translation: AAH19447.1.
BC033377 mRNA. Translation: AAH33377.1.
CCDSiCCDS15411.1.
RefSeqiNP_543120.1. NM_080844.4.
XP_006496689.1. XM_006496626.1.
UniGeneiMm.260770.

Genome annotation databases

EnsembliENSMUST00000064725; ENSMUSP00000068971; ENSMUSG00000026715.
GeneIDi11905.
KEGGimmu:11905.
UCSCiuc007dem.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S47225 mRNA. Translation: AAB23965.1.
BC019447 mRNA. Translation: AAH19447.1.
BC033377 mRNA. Translation: AAH33377.1.
CCDSiCCDS15411.1.
RefSeqiNP_543120.1. NM_080844.4.
XP_006496689.1. XM_006496626.1.
UniGeneiMm.260770.

3D structure databases

ProteinModelPortaliP32261.
SMRiP32261. Positions 39-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32261. 3 interactions.
MINTiMINT-1846278.
STRINGi10090.ENSMUSP00000068971.

Protein family/group databases

MEROPSiI04.018.

PTM databases

PhosphoSiteiP32261.

Proteomic databases

MaxQBiP32261.
PaxDbiP32261.
PRIDEiP32261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064725; ENSMUSP00000068971; ENSMUSG00000026715.
GeneIDi11905.
KEGGimmu:11905.
UCSCiuc007dem.2. mouse.

Organism-specific databases

CTDi462.
MGIiMGI:88095. Serpinc1.

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP32261.
KOiK03911.
OMAiWGCVTCH.
OrthoDBiEOG7327PB.
PhylomeDBiP32261.
TreeFamiTF343094.

Enzyme and pathway databases

ReactomeiREACT_286713. Common Pathway of Fibrin Clot Formation.
REACT_340184. Intrinsic Pathway of Fibrin Clot Formation.

Miscellaneous databases

NextBioi279953.
PROiP32261.
SOURCEiSearch...

Gene expression databases

BgeeiP32261.
ExpressionAtlasiP32261. baseline and differential.
GenevisibleiP32261. MM.

Family and domain databases

InterProiIPR015555. AT-III.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PTHR11461:SF53. PTHR11461:SF53. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and cell-free expression of mouse antithrombin III."
    Wu J.K., Sheffield W.P., Blajchman M.A.
    Thromb. Haemost. 68:291-296(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-188.
    Strain: C57BL/6.
    Tissue: Plasma.
  4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129; ASN-168 AND ASN-188.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiANT3_MOUSE
AccessioniPrimary (citable) accession number: P32261
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 24, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.