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P32259 (MED16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 16
Alternative name(s):
Global transcriptional regulator SIN4
Mediator complex subunit 16
SNF1 suppressor protein 4
SWI4 suppressor protein 5
Transcriptional silencing factor 3
YGP1 expression regulatory protein 1
Gene names
Name:SIN4
Synonyms:BEL2, GAL22, MED16, RYE1, SDI3, SSF5, SSN4, TSF3
Ordered Locus Names:YNL236W
ORF Names:N1135
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length974 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.15 Ref.16 Ref.17

Subunit structure

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Interacts with HOG1. Ref.8 Ref.18

Subcellular location

Nucleus Ref.9.

Post-translational modification

Phosphorylated by KIN28. Ref.12

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Mediator complex subunit 16 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 974974Mediator of RNA polymerase II transcription subunit 16
PRO_0000096364

Regions

Motif889 – 8935Nuclear localization signal Potential
Compositional bias63 – 8119Ser/Thr-rich

Sequences

Sequence LengthMass (Da)Tools
P32259 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 12ECF5E4CDC05A8E

FASTA974111,297
        10         20         30         40         50         60 
MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV LHPQLHEVQY 

        70         80         90        100        110        120 
QESSSTLSTH STTTSVNGST TAGVGSTPNF GGNSNKSPPQ FFYNISSIHW NNWFSLPGDM 

       130        140        150        160        170        180 
LAVCDELGNM TMLITGQRPD RATTYEKLTM VFQDNVYKIY NHVMPLKPVD KLKPMNIERK 

       190        200        210        220        230        240 
QTRKEYNTSI LEFRWLTSSK SVIVSQFCAF DSSSNTYRSR AQQVPPYGVY HPPFIKYACL 

       250        260        270        280        290        300 
AIRKNGQIDF WYQFSNSKDH KKITLQLLDT SNQRFKDLQW LEFARITPMN DDQCMLITTY 

       310        320        330        340        350        360 
SKLSKNISFY KLHVNWNLNA TKPNVLNDPS LKIQFILSTT LDPTDDEGHV LKLENLHVVS 

       370        380        390        400        410        420 
KSSIEKDPSP EILVLYNVCD TSKSLVKRYR LAPTQLSAEY LVILKPDLNI DRNNSTNQIF 

       430        440        450        460        470        480 
QSRRYNLRRH SDIVLDKKVT LITSEMFDAF VSFYFEDGTI ESYNQNDWKL ETERLISQSQ 

       490        500        510        520        530        540 
LGKFKNIIAS PLSAGFNYGK LPLPPSVEWM KVSPSMCGVI VKQYNKKWPQ FYAAVQKNYA 

       550        560        570        580        590        600 
DPEKDSINAT ALAFGYVKSL HKQISAEDLT IAAKTHILRI SFLDRKRAKE FITTLLKSLY 

       610        620        630        640        650        660 
SFFNISPDAP KEIMDKIITS RPLQKIMLLQ LELGSCFSQE NIEEMARVIL YLKNVLFAFN 

       670        680        690        700        710        720 
GVARNFHFAI EQISNNSNQQ QNPKLFQTIF SKQDLIHSLI PVAKWFVKFI TYLTQEILIL 

       730        740        750        760        770        780 
INDPTNKEYT LVHGIFGAKM SRTLILSILN EIKKVTQIVA KFPETSYPIL NESSTFLKLV 

       790        800        810        820        830        840 
LSESPVDFEK FETFLVDVNN KFIALCEQQP SQEREFSLLV KAEIPPEYAK VGDFLLQYAN 

       850        860        870        880        890        900 
NAVISHANAA AVYFADTSGL KISNSEFFNP EIFHLLQPLE EGLIIDTDKL PIKNRTSKSF 

       910        920        930        940        950        960 
SKLLYDDVTC DKLSVSEISD GKLKRCSRCG SVTRAGNIIS SDKTIVPTSI QTKRWPTMYT 

       970 
RLCICSGMLF EMDG 

« Hide

References

« Hide 'large scale' references
[1]"TSF3, a global regulatory protein that silences transcription of yeast GAL genes, also mediates repression by alpha 2 repressor and is identical to SIN4."
Chen S., West R.W. Jr., Johnson S.L., Gans H., Kruger B., Ma J.
Mol. Cell. Biol. 13:831-840(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YM256.
[2]"Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae."
Jiang Y.W., Stillman D.J.
Mol. Cell. Biol. 12:4503-4514(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"BEL2/SIN4/TSF3 encodes a position-dependent general repressor of a diverse set of genes in yeast."
Harashima S., Mabuchi H., Ramash R., Hasebe M., Tanaka A., Oshima Y.
Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme."
Li Y., Bjoerklund S., Jiang Y.W., Kim Y.-J., Lane W.S., Stillman D.J., Kornberg R.D.
Proc. Natl. Acad. Sci. U.S.A. 92:10864-10868(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPONENT OF MEDIATOR COMPLEX.
[8]"Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HOG1.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II."
Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. expand/collapse author list , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D.
Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[12]"Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex."
Liu Y., Kung C., Fishburn J., Ansari A.Z., Shokat K.M., Hahn S.
Mol. Cell. Biol. 24:1721-1735(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY KIN28.
[13]"A high resolution protein interaction map of the yeast Mediator complex."
Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.
Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE OF THE MEDIATOR COMPLEX.
[14]"Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[15]"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
Nair D., Kim Y., Myers L.C.
J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[16]"Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Mediator as a general transcription factor."
Takagi Y., Kornberg R.D.
J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[18]"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
Baidoobonso S.M., Guidi B.W., Myers L.C.
J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[19]"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93050 Genomic DNA. Translation: AAA35044.1.
X64516 Genomic DNA. Translation: CAA45819.1.
D12918 Genomic DNA. Translation: BAA02302.1.
Z69381 Genomic DNA. Translation: CAA93362.1.
Z71512 Genomic DNA. Translation: CAA96140.1.
BK006947 Genomic DNA. Translation: DAA10323.1.
PIRA44484.
RefSeqNP_014163.1. NM_001183074.1.

3D structure databases

ProteinModelPortalP32259.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35603. 388 interactions.
DIPDIP-1617N.
IntActP32259. 37 interactions.
MINTMINT-386177.
STRING4932.YNL236W.

Proteomic databases

PaxDbP32259.
PeptideAtlasP32259.
PRIDEP32259.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL236W; YNL236W; YNL236W.
GeneID855485.
KEGGsce:YNL236W.

Organism-specific databases

CYGDYNL236w.
SGDS000005180. SIN4.

Phylogenomic databases

eggNOGNOG297401.
HOGENOMHOG000113525.
KOK15160.
OMAIYRQISK.
OrthoDBEOG780RVT.

Enzyme and pathway databases

BioCycYEAST:G3O-33235-MONOMER.

Gene expression databases

GenevestigatorP32259.

Family and domain databases

InterProIPR021665. Mediator_Med16.
[Graphical view]
PfamPF11635. Med16. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979460.

Entry information

Entry nameMED16_YEAST
AccessionPrimary (citable) accession number: P32259
Secondary accession number(s): D6W0V7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families