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P32259

- MED16_YEAST

UniProt

P32259 - MED16_YEAST

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Protein
Mediator of RNA polymerase II transcription subunit 16
Gene
SIN4, BEL2, GAL22, MED16, RYE1, SDI3, SSF5, SSN4, TSF3, YNL236W, N1135
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.3 Publications

GO - Molecular functioni

  1. RNA polymerase II activating transcription factor binding Source: SGD

GO - Biological processi

  1. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33235-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 16
Alternative name(s):
Global transcriptional regulator SIN4
Mediator complex subunit 16
SNF1 suppressor protein 4
SWI4 suppressor protein 5
Transcriptional silencing factor 3
YGP1 expression regulatory protein 1
Gene namesi
Name:SIN4
Synonyms:BEL2, GAL22, MED16, RYE1, SDI3, SSF5, SSN4, TSF3
Ordered Locus Names:YNL236W
ORF Names:N1135
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL236w.
SGDiS000005180. SIN4.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. core mediator complex Source: SGD
  2. mediator complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 974974Mediator of RNA polymerase II transcription subunit 16
PRO_0000096364Add
BLAST

Post-translational modificationi

Phosphorylated by KIN28.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32259.
PaxDbiP32259.
PeptideAtlasiP32259.
PRIDEiP32259.

Expressioni

Gene expression databases

GenevestigatoriP32259.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Interacts with HOG1.2 Publications

Protein-protein interaction databases

BioGridi35603. 388 interactions.
DIPiDIP-1617N.
IntActiP32259. 37 interactions.
MINTiMINT-386177.
STRINGi4932.YNL236W.

Structurei

3D structure databases

ProteinModelPortaliP32259.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi889 – 8935Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 8119Ser/Thr-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG297401.
HOGENOMiHOG000113525.
KOiK15160.
OMAiIYRQISK.
OrthoDBiEOG780RVT.

Family and domain databases

InterProiIPR021665. Mediator_Med16.
[Graphical view]
PfamiPF11635. Med16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32259-1 [UniParc]FASTAAdd to Basket

« Hide

MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV    50
LHPQLHEVQY QESSSTLSTH STTTSVNGST TAGVGSTPNF GGNSNKSPPQ 100
FFYNISSIHW NNWFSLPGDM LAVCDELGNM TMLITGQRPD RATTYEKLTM 150
VFQDNVYKIY NHVMPLKPVD KLKPMNIERK QTRKEYNTSI LEFRWLTSSK 200
SVIVSQFCAF DSSSNTYRSR AQQVPPYGVY HPPFIKYACL AIRKNGQIDF 250
WYQFSNSKDH KKITLQLLDT SNQRFKDLQW LEFARITPMN DDQCMLITTY 300
SKLSKNISFY KLHVNWNLNA TKPNVLNDPS LKIQFILSTT LDPTDDEGHV 350
LKLENLHVVS KSSIEKDPSP EILVLYNVCD TSKSLVKRYR LAPTQLSAEY 400
LVILKPDLNI DRNNSTNQIF QSRRYNLRRH SDIVLDKKVT LITSEMFDAF 450
VSFYFEDGTI ESYNQNDWKL ETERLISQSQ LGKFKNIIAS PLSAGFNYGK 500
LPLPPSVEWM KVSPSMCGVI VKQYNKKWPQ FYAAVQKNYA DPEKDSINAT 550
ALAFGYVKSL HKQISAEDLT IAAKTHILRI SFLDRKRAKE FITTLLKSLY 600
SFFNISPDAP KEIMDKIITS RPLQKIMLLQ LELGSCFSQE NIEEMARVIL 650
YLKNVLFAFN GVARNFHFAI EQISNNSNQQ QNPKLFQTIF SKQDLIHSLI 700
PVAKWFVKFI TYLTQEILIL INDPTNKEYT LVHGIFGAKM SRTLILSILN 750
EIKKVTQIVA KFPETSYPIL NESSTFLKLV LSESPVDFEK FETFLVDVNN 800
KFIALCEQQP SQEREFSLLV KAEIPPEYAK VGDFLLQYAN NAVISHANAA 850
AVYFADTSGL KISNSEFFNP EIFHLLQPLE EGLIIDTDKL PIKNRTSKSF 900
SKLLYDDVTC DKLSVSEISD GKLKRCSRCG SVTRAGNIIS SDKTIVPTSI 950
QTKRWPTMYT RLCICSGMLF EMDG 974
Length:974
Mass (Da):111,297
Last modified:October 1, 1993 - v1
Checksum:i12ECF5E4CDC05A8E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93050 Genomic DNA. Translation: AAA35044.1.
X64516 Genomic DNA. Translation: CAA45819.1.
D12918 Genomic DNA. Translation: BAA02302.1.
Z69381 Genomic DNA. Translation: CAA93362.1.
Z71512 Genomic DNA. Translation: CAA96140.1.
BK006947 Genomic DNA. Translation: DAA10323.1.
PIRiA44484.
RefSeqiNP_014163.1. NM_001183074.1.

Genome annotation databases

EnsemblFungiiYNL236W; YNL236W; YNL236W.
GeneIDi855485.
KEGGisce:YNL236W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93050 Genomic DNA. Translation: AAA35044.1 .
X64516 Genomic DNA. Translation: CAA45819.1 .
D12918 Genomic DNA. Translation: BAA02302.1 .
Z69381 Genomic DNA. Translation: CAA93362.1 .
Z71512 Genomic DNA. Translation: CAA96140.1 .
BK006947 Genomic DNA. Translation: DAA10323.1 .
PIRi A44484.
RefSeqi NP_014163.1. NM_001183074.1.

3D structure databases

ProteinModelPortali P32259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35603. 388 interactions.
DIPi DIP-1617N.
IntActi P32259. 37 interactions.
MINTi MINT-386177.
STRINGi 4932.YNL236W.

Proteomic databases

MaxQBi P32259.
PaxDbi P32259.
PeptideAtlasi P32259.
PRIDEi P32259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL236W ; YNL236W ; YNL236W .
GeneIDi 855485.
KEGGi sce:YNL236W.

Organism-specific databases

CYGDi YNL236w.
SGDi S000005180. SIN4.

Phylogenomic databases

eggNOGi NOG297401.
HOGENOMi HOG000113525.
KOi K15160.
OMAi IYRQISK.
OrthoDBi EOG780RVT.

Enzyme and pathway databases

BioCyci YEAST:G3O-33235-MONOMER.

Miscellaneous databases

NextBioi 979460.

Gene expression databases

Genevestigatori P32259.

Family and domain databases

InterProi IPR021665. Mediator_Med16.
[Graphical view ]
Pfami PF11635. Med16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TSF3, a global regulatory protein that silences transcription of yeast GAL genes, also mediates repression by alpha 2 repressor and is identical to SIN4."
    Chen S., West R.W. Jr., Johnson S.L., Gans H., Kruger B., Ma J.
    Mol. Cell. Biol. 13:831-840(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YM256.
  2. "Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae."
    Jiang Y.W., Stillman D.J.
    Mol. Cell. Biol. 12:4503-4514(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "BEL2/SIN4/TSF3 encodes a position-dependent general repressor of a diverse set of genes in yeast."
    Harashima S., Mabuchi H., Ramash R., Hasebe M., Tanaka A., Oshima Y.
    Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
    Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
    Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme."
    Li Y., Bjoerklund S., Jiang Y.W., Kim Y.-J., Lane W.S., Stillman D.J., Kornberg R.D.
    Proc. Natl. Acad. Sci. U.S.A. 92:10864-10868(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF MEDIATOR COMPLEX.
  8. "Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
    Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
    EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HOG1.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. Cited for: NOMENCLATURE.
  12. "Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex."
    Liu Y., Kung C., Fishburn J., Ansari A.Z., Shokat K.M., Hahn S.
    Mol. Cell. Biol. 24:1721-1735(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY KIN28.
  13. Cited for: STRUCTURE OF THE MEDIATOR COMPLEX.
  14. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
    Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
    J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
  15. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
    Nair D., Kim Y., Myers L.C.
    J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  16. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
    van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
    Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Mediator as a general transcription factor."
    Takagi Y., Kornberg R.D.
    J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  18. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
    Baidoobonso S.M., Guidi B.W., Myers L.C.
    J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  19. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
    Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
    Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED16_YEAST
AccessioniPrimary (citable) accession number: P32259
Secondary accession number(s): D6W0V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi