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P32259

- MED16_YEAST

UniProt

P32259 - MED16_YEAST

Protein

Mediator of RNA polymerase II transcription subunit 16

Gene

SIN4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.3 Publications

    GO - Molecular functioni

    1. RNA polymerase II activating transcription factor binding Source: SGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33235-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 16
    Alternative name(s):
    Global transcriptional regulator SIN4
    Mediator complex subunit 16
    SNF1 suppressor protein 4
    SWI4 suppressor protein 5
    Transcriptional silencing factor 3
    YGP1 expression regulatory protein 1
    Gene namesi
    Name:SIN4
    Synonyms:BEL2, GAL22, MED16, RYE1, SDI3, SSF5, SSN4, TSF3
    Ordered Locus Names:YNL236W
    ORF Names:N1135
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL236w.
    SGDiS000005180. SIN4.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. core mediator complex Source: SGD
    2. mediator complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 974974Mediator of RNA polymerase II transcription subunit 16PRO_0000096364Add
    BLAST

    Post-translational modificationi

    Phosphorylated by KIN28.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32259.
    PaxDbiP32259.
    PeptideAtlasiP32259.
    PRIDEiP32259.

    Expressioni

    Gene expression databases

    GenevestigatoriP32259.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Interacts with HOG1.2 Publications

    Protein-protein interaction databases

    BioGridi35603. 388 interactions.
    DIPiDIP-1617N.
    IntActiP32259. 37 interactions.
    MINTiMINT-386177.
    STRINGi4932.YNL236W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32259.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi889 – 8935Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi63 – 8119Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 16 family.Curated

    Phylogenomic databases

    eggNOGiNOG297401.
    HOGENOMiHOG000113525.
    KOiK15160.
    OMAiIYRQISK.
    OrthoDBiEOG780RVT.

    Family and domain databases

    InterProiIPR021665. Mediator_Med16.
    [Graphical view]
    PfamiPF11635. Med16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32259-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV    50
    LHPQLHEVQY QESSSTLSTH STTTSVNGST TAGVGSTPNF GGNSNKSPPQ 100
    FFYNISSIHW NNWFSLPGDM LAVCDELGNM TMLITGQRPD RATTYEKLTM 150
    VFQDNVYKIY NHVMPLKPVD KLKPMNIERK QTRKEYNTSI LEFRWLTSSK 200
    SVIVSQFCAF DSSSNTYRSR AQQVPPYGVY HPPFIKYACL AIRKNGQIDF 250
    WYQFSNSKDH KKITLQLLDT SNQRFKDLQW LEFARITPMN DDQCMLITTY 300
    SKLSKNISFY KLHVNWNLNA TKPNVLNDPS LKIQFILSTT LDPTDDEGHV 350
    LKLENLHVVS KSSIEKDPSP EILVLYNVCD TSKSLVKRYR LAPTQLSAEY 400
    LVILKPDLNI DRNNSTNQIF QSRRYNLRRH SDIVLDKKVT LITSEMFDAF 450
    VSFYFEDGTI ESYNQNDWKL ETERLISQSQ LGKFKNIIAS PLSAGFNYGK 500
    LPLPPSVEWM KVSPSMCGVI VKQYNKKWPQ FYAAVQKNYA DPEKDSINAT 550
    ALAFGYVKSL HKQISAEDLT IAAKTHILRI SFLDRKRAKE FITTLLKSLY 600
    SFFNISPDAP KEIMDKIITS RPLQKIMLLQ LELGSCFSQE NIEEMARVIL 650
    YLKNVLFAFN GVARNFHFAI EQISNNSNQQ QNPKLFQTIF SKQDLIHSLI 700
    PVAKWFVKFI TYLTQEILIL INDPTNKEYT LVHGIFGAKM SRTLILSILN 750
    EIKKVTQIVA KFPETSYPIL NESSTFLKLV LSESPVDFEK FETFLVDVNN 800
    KFIALCEQQP SQEREFSLLV KAEIPPEYAK VGDFLLQYAN NAVISHANAA 850
    AVYFADTSGL KISNSEFFNP EIFHLLQPLE EGLIIDTDKL PIKNRTSKSF 900
    SKLLYDDVTC DKLSVSEISD GKLKRCSRCG SVTRAGNIIS SDKTIVPTSI 950
    QTKRWPTMYT RLCICSGMLF EMDG 974
    Length:974
    Mass (Da):111,297
    Last modified:October 1, 1993 - v1
    Checksum:i12ECF5E4CDC05A8E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93050 Genomic DNA. Translation: AAA35044.1.
    X64516 Genomic DNA. Translation: CAA45819.1.
    D12918 Genomic DNA. Translation: BAA02302.1.
    Z69381 Genomic DNA. Translation: CAA93362.1.
    Z71512 Genomic DNA. Translation: CAA96140.1.
    BK006947 Genomic DNA. Translation: DAA10323.1.
    PIRiA44484.
    RefSeqiNP_014163.1. NM_001183074.1.

    Genome annotation databases

    EnsemblFungiiYNL236W; YNL236W; YNL236W.
    GeneIDi855485.
    KEGGisce:YNL236W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93050 Genomic DNA. Translation: AAA35044.1 .
    X64516 Genomic DNA. Translation: CAA45819.1 .
    D12918 Genomic DNA. Translation: BAA02302.1 .
    Z69381 Genomic DNA. Translation: CAA93362.1 .
    Z71512 Genomic DNA. Translation: CAA96140.1 .
    BK006947 Genomic DNA. Translation: DAA10323.1 .
    PIRi A44484.
    RefSeqi NP_014163.1. NM_001183074.1.

    3D structure databases

    ProteinModelPortali P32259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35603. 388 interactions.
    DIPi DIP-1617N.
    IntActi P32259. 37 interactions.
    MINTi MINT-386177.
    STRINGi 4932.YNL236W.

    Proteomic databases

    MaxQBi P32259.
    PaxDbi P32259.
    PeptideAtlasi P32259.
    PRIDEi P32259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL236W ; YNL236W ; YNL236W .
    GeneIDi 855485.
    KEGGi sce:YNL236W.

    Organism-specific databases

    CYGDi YNL236w.
    SGDi S000005180. SIN4.

    Phylogenomic databases

    eggNOGi NOG297401.
    HOGENOMi HOG000113525.
    KOi K15160.
    OMAi IYRQISK.
    OrthoDBi EOG780RVT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33235-MONOMER.

    Miscellaneous databases

    NextBioi 979460.

    Gene expression databases

    Genevestigatori P32259.

    Family and domain databases

    InterProi IPR021665. Mediator_Med16.
    [Graphical view ]
    Pfami PF11635. Med16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TSF3, a global regulatory protein that silences transcription of yeast GAL genes, also mediates repression by alpha 2 repressor and is identical to SIN4."
      Chen S., West R.W. Jr., Johnson S.L., Gans H., Kruger B., Ma J.
      Mol. Cell. Biol. 13:831-840(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YM256.
    2. "Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae."
      Jiang Y.W., Stillman D.J.
      Mol. Cell. Biol. 12:4503-4514(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "BEL2/SIN4/TSF3 encodes a position-dependent general repressor of a diverse set of genes in yeast."
      Harashima S., Mabuchi H., Ramash R., Hasebe M., Tanaka A., Oshima Y.
      Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
      Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
      Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme."
      Li Y., Bjoerklund S., Jiang Y.W., Kim Y.-J., Lane W.S., Stillman D.J., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 92:10864-10868(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPONENT OF MEDIATOR COMPLEX.
    8. "Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
      Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
      EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOG1.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. Cited for: NOMENCLATURE.
    12. "Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex."
      Liu Y., Kung C., Fishburn J., Ansari A.Z., Shokat K.M., Hahn S.
      Mol. Cell. Biol. 24:1721-1735(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY KIN28.
    13. Cited for: STRUCTURE OF THE MEDIATOR COMPLEX.
    14. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
      Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
      J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
    15. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    16. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
      van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
      Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    17. "Mediator as a general transcription factor."
      Takagi Y., Kornberg R.D.
      J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    18. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
      Baidoobonso S.M., Guidi B.W., Myers L.C.
      J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    19. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
      Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
      Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED16_YEAST
    AccessioniPrimary (citable) accession number: P32259
    Secondary accession number(s): D6W0V7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1720 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3