Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tubulin alpha chain

Gene

tubA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei457Involved in polymerization1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi148 – 154GTPSequence analysis7

GO - Molecular functioni

GO - Biological processi

  • chromosome segregation Source: dictyBase
  • microtubule-based process Source: InterPro

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-114608. Platelet degranulation.
R-DDI-6807878. COPI-mediated anterograde transport.
R-DDI-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-DDI-8955332. Carboxyterminal post-translational modifications of tubulin.
R-DDI-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin alpha chain
Alternative name(s):
Alpha-tubulin
Gene namesi
Name:tubA
ORF Names:DDB_G0287689
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287689. tubA.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: dictyBase
  • microtubule Source: dictyBase
  • phagocytic vesicle Source: dictyBase

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000481571 – 457Tubulin alpha chainAdd BLAST457

Post-translational modificationi

Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.By similarity

Proteomic databases

PaxDbiP32255.
PRIDEiP32255.

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Protein-protein interaction databases

IntActiP32255. 1 interactor.
STRINGi44689.DDB0191380.

Structurei

3D structure databases

ProteinModelPortaliP32255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiKOG1376. Eukaryota.
COG5023. LUCA.
InParanoidiP32255.
KOiK07374.
OMAiGICYQAP.
PhylomeDBiP32255.

Family and domain databases

Gene3Di3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiView protein in InterPro
IPR002452. Alpha_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR037103. Tubulin/FtsZ_C_sf.
IPR036525. Tubulin/FtsZ_GTPase_sf.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiView protein in Pfam
PF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
PRINTSiPR01162. ALPHATUBULIN.
PR01161. TUBULIN.
SMARTiView protein in SMART
SM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiView protein in PROSITE
PS00227. TUBULIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P32255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGREIISIHI GQAGVQVGNS CWELYCLEHG IERDGSIPAD RKQSSDVNNL
60 70 80 90 100
NKDLGTFFSE STNGKKVVPR AIFLDLEPTV IDEIRTGDYK NLFHPEQLIT
110 120 130 140 150
GKEDAANNYA RGHYTVGKEL IDVCVDRIRR LADQCDGLQG FLVFHSVGGG
160 170 180 190 200
TGSGFGSLLL QKLALDYGGK KSKLDFCVYP SPQVSTSVVE PYNSVLSTHS
210 220 230 240 250
LLEHTDVSFM LDNEAIYNIC KNSLDIEKPT YTNLNRLIAQ VISSLTSSLR
260 270 280 290 300
FPGQLNLDIN DIQTNLVPFP RLHFVLCSYA PVISREKAHH ETITVDNITS
310 320 330 340 350
AVFSEKNIMA KCQPNLGKYM ACCLMYRGDI VPKEAQKAVQ NIRSEKSRNV
360 370 380 390 400
SFVDWSPTGF KCGINNQAPV STKDSEMAEV KKSVCMLSNT TAISQVFSRI
410 420 430 440 450
NHKFDLMFVK RAFVHWYVGE GMEEGEFAEA RDDLLALEKD YESVSASTEG

EEQEEEY
Length:457
Mass (Da):50,961
Last modified:October 1, 1993 - v1
Checksum:iD1F2F588BF3859E6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti209 – 210FM → LV in strain: V34. 2
Natural varianti288A → Y in strain: V34. 1
Natural varianti427 – 428FA → GL in strain: V34. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13999 mRNA. Translation: AAC37343.1.
AAFI02000104 Genomic DNA. Translation: EAL63491.1.
AM168454 Genomic DNA. Translation: CAJ44836.1.
RefSeqiXP_637058.1. XM_631966.1.

Genome annotation databases

EnsemblProtistsiEAL63491; EAL63491; DDB_G0287689.
GeneIDi8626312.
KEGGiddi:DDB_G0287689.

Similar proteinsi

Entry informationi

Entry nameiTBA_DICDI
AccessioniPrimary (citable) accession number: P32255
Secondary accession number(s): A0AAL2, Q54JU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 25, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families