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Protein

Tubulin alpha chain

Gene

tubA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei457Involved in polymerization1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi148 – 154GTPSequence analysis7

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: dictyBase

GO - Biological processi

  • chromosome segregation Source: dictyBase
  • microtubule-based process Source: InterPro

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-114608 Platelet degranulation
R-DDI-6807878 COPI-mediated anterograde transport
R-DDI-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-DDI-8955332 Carboxyterminal post-translational modifications of tubulin
R-DDI-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin alpha chain
Alternative name(s):
Alpha-tubulin
Gene namesi
Name:tubA
ORF Names:DDB_G0287689
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287689 tubA

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: dictyBase
  • cytoskeleton Source: dictyBase
  • microtubule Source: dictyBase
  • phagocytic vesicle Source: dictyBase

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000481571 – 457Tubulin alpha chainAdd BLAST457

Post-translational modificationi

Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.By similarity

Proteomic databases

PaxDbiP32255
PRIDEiP32255

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Protein-protein interaction databases

IntActiP32255, 1 interactor
STRINGi44689.DDB0191380

Structurei

3D structure databases

ProteinModelPortaliP32255
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiKOG1376 Eukaryota
COG5023 LUCA
InParanoidiP32255
KOiK07374
OMAiGICYQAP
PhylomeDBiP32255

Family and domain databases

Gene3Di1.10.287.600, 1 hit
3.30.1330.20, 1 hit
3.40.50.1440, 1 hit
InterProiView protein in InterPro
IPR002452 Alpha_tubulin
IPR008280 Tub_FtsZ_C
IPR000217 Tubulin
IPR018316 Tubulin/FtsZ_2-layer-sand-dom
IPR037103 Tubulin/FtsZ_C_sf
IPR036525 Tubulin/FtsZ_GTPase_sf
IPR023123 Tubulin_C
IPR017975 Tubulin_CS
IPR003008 Tubulin_FtsZ_GTPase
PANTHERiPTHR11588 PTHR11588, 1 hit
PfamiView protein in Pfam
PF00091 Tubulin, 1 hit
PF03953 Tubulin_C, 1 hit
PRINTSiPR01162 ALPHATUBULIN
PR01161 TUBULIN
SMARTiView protein in SMART
SM00864 Tubulin, 1 hit
SM00865 Tubulin_C, 1 hit
SUPFAMiSSF52490 SSF52490, 1 hit
SSF55307 SSF55307, 1 hit
PROSITEiView protein in PROSITE
PS00227 TUBULIN, 1 hit

Sequencei

Sequence statusi: Complete.

P32255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGREIISIHI GQAGVQVGNS CWELYCLEHG IERDGSIPAD RKQSSDVNNL
60 70 80 90 100
NKDLGTFFSE STNGKKVVPR AIFLDLEPTV IDEIRTGDYK NLFHPEQLIT
110 120 130 140 150
GKEDAANNYA RGHYTVGKEL IDVCVDRIRR LADQCDGLQG FLVFHSVGGG
160 170 180 190 200
TGSGFGSLLL QKLALDYGGK KSKLDFCVYP SPQVSTSVVE PYNSVLSTHS
210 220 230 240 250
LLEHTDVSFM LDNEAIYNIC KNSLDIEKPT YTNLNRLIAQ VISSLTSSLR
260 270 280 290 300
FPGQLNLDIN DIQTNLVPFP RLHFVLCSYA PVISREKAHH ETITVDNITS
310 320 330 340 350
AVFSEKNIMA KCQPNLGKYM ACCLMYRGDI VPKEAQKAVQ NIRSEKSRNV
360 370 380 390 400
SFVDWSPTGF KCGINNQAPV STKDSEMAEV KKSVCMLSNT TAISQVFSRI
410 420 430 440 450
NHKFDLMFVK RAFVHWYVGE GMEEGEFAEA RDDLLALEKD YESVSASTEG

EEQEEEY
Length:457
Mass (Da):50,961
Last modified:October 1, 1993 - v1
Checksum:iD1F2F588BF3859E6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti209 – 210FM → LV in strain: V34. 2
Natural varianti288A → Y in strain: V34. 1
Natural varianti427 – 428FA → GL in strain: V34. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13999 mRNA Translation: AAC37343.1
AAFI02000104 Genomic DNA Translation: EAL63491.1
AM168454 Genomic DNA Translation: CAJ44836.1
RefSeqiXP_637058.1, XM_631966.1

Genome annotation databases

EnsemblProtistsiEAL63491; EAL63491; DDB_G0287689
GeneIDi8626312
KEGGiddi:DDB_G0287689

Similar proteinsi

Entry informationi

Entry nameiTBA_DICDI
AccessioniPrimary (citable) accession number: P32255
Secondary accession number(s): A0AAL2, Q54JU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

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