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P32249

- GP183_HUMAN

UniProt

P32249 - GP183_HUMAN

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Protein

G-protein coupled receptor 183

Gene
GPR183, EBI2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses By similarity. Signals constitutively through G(i)-alpha, but not through G(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2).2 Publications

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: ProtInc
  2. oxysterol binding Source: UniProtKB

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: UniProtKB
  2. humoral immune response Source: UniProtKB
  3. immune response Source: ProtInc
  4. mature B cell differentiation involved in immune response Source: UniProtKB
  5. positive regulation of B cell proliferation Source: UniProtKB
  6. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 183
Alternative name(s):
Epstein-Barr virus-induced G-protein coupled receptor 2
Short name:
EBI2
Short name:
EBV-induced G-protein coupled receptor 2
Gene namesi
Name:GPR183
Synonyms:EBI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 13

Organism-specific databases

HGNCiHGNC:3128. GPR183.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131Extracellular Reviewed predictionAdd
BLAST
Transmembranei32 – 5726Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini58 – 7720Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei78 – 9518Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini96 – 10510Extracellular Reviewed prediction
Transmembranei106 – 12722Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini128 – 14922Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei150 – 16819Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini169 – 19224Extracellular Reviewed predictionAdd
BLAST
Transmembranei193 – 21523Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini216 – 24126Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei242 – 26524Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini266 – 28722Extracellular Reviewed predictionAdd
BLAST
Transmembranei288 – 31225Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini313 – 36149Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi111 – 1111F → A: 500-fold decrease of IC(50) for GSK682753A. 1 Publication

Organism-specific databases

PharmGKBiPA162390174.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361G-protein coupled receptor 183PRO_0000069411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 181 By similarity
Modified residuei328 – 3281Phosphoserine By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP32249.
PaxDbiP32249.
PeptideAtlasiP32249.
PRIDEiP32249.

PTM databases

PhosphoSiteiP32249.

Expressioni

Tissue specificityi

Expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. Also highly expressed in lung, heart and gastrointestinal tract, and weakly expressed in the urogenital system and brain.2 Publications

Inductioni

By Epstein-Barr virus (EBV).1 Publication

Gene expression databases

BgeeiP32249.
CleanExiHS_GPR183.
GenevestigatoriP32249.

Organism-specific databases

HPAiCAB033690.
HPA028847.

Interactioni

Protein-protein interaction databases

BioGridi108212. 2 interactions.
IntActiP32249. 2 interactions.
MINTiMINT-1427405.
STRINGi9606.ENSP00000365596.

Structurei

3D structure databases

ProteinModelPortaliP32249.
SMRiP32249. Positions 20-323.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1349Interaction with G proteins

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145860.
HOGENOMiHOG000043070.
HOVERGENiHBG101355.
InParanoidiP32249.
KOiK04305.
OMAiLVFYINT.
OrthoDBiEOG7VDXPF.
PhylomeDBiP32249.
TreeFamiTF350009.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32249-1 [UniParc]FASTAAdd to Basket

« Hide

MDIQMANNFT PPSATPQGND CDLYAHHSTA RIVMPLHYSL VFIIGLVGNL    50
LALVVIVQNR KKINSTTLYS TNLVISDILF TTALPTRIAY YAMGFDWRIG 100
DALCRITALV FYINTYAGVN FMTCLSIDRF IAVVHPLRYN KIKRIEHAKG 150
VCIFVWILVF AQTLPLLINP MSKQEAERIT CMEYPNFEET KSLPWILLGA 200
CFIGYVLPLI IILICYSQIC CKLFRTAKQN PLTEKSGVNK KALNTIILII 250
VVFVLCFTPY HVAIIQHMIK KLRFSNFLEC SQRHSFQISL HFTVCLMNFN 300
CCMDPFIYFF ACKGYKRKVM RMLKRQVSVS ISSAVKSAPE ENSREMTETQ 350
MMIHSKSSNG K 361
Length:361
Mass (Da):41,224
Last modified:September 2, 2008 - v3
Checksum:iB5A2171F34C9C67B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti338 – 3381A → V in an acute myeloid leukemia sample; somatic mutation. 1 Publication
VAR_054147

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → V in BAD97110. 1 Publication
Sequence conflicti179 – 1791I → N in BAG36232. 1 Publication
Sequence conflicti325 – 3251R → Q in BAG36232. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08177 mRNA. Translation: AAA35924.1.
AK292091 mRNA. Translation: BAF84780.1.
AK313443 mRNA. Translation: BAG36232.1.
AK223390 mRNA. Translation: BAD97110.1.
AL160155 Genomic DNA. Translation: CAI14308.1.
CH471085 Genomic DNA. Translation: EAX09018.1.
BC020752 mRNA. Translation: AAH20752.1.
CCDSiCCDS9492.1.
PIRiB45680.
RefSeqiNP_004942.1. NM_004951.4.
UniGeneiHs.784.

Genome annotation databases

EnsembliENST00000376414; ENSP00000365596; ENSG00000169508.
GeneIDi1880.
KEGGihsa:1880.
UCSCiuc001vog.3. human.

Polymorphism databases

DMDMi205371788.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08177 mRNA. Translation: AAA35924.1 .
AK292091 mRNA. Translation: BAF84780.1 .
AK313443 mRNA. Translation: BAG36232.1 .
AK223390 mRNA. Translation: BAD97110.1 .
AL160155 Genomic DNA. Translation: CAI14308.1 .
CH471085 Genomic DNA. Translation: EAX09018.1 .
BC020752 mRNA. Translation: AAH20752.1 .
CCDSi CCDS9492.1.
PIRi B45680.
RefSeqi NP_004942.1. NM_004951.4.
UniGenei Hs.784.

3D structure databases

ProteinModelPortali P32249.
SMRi P32249. Positions 20-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 108212. 2 interactions.
IntActi P32249. 2 interactions.
MINTi MINT-1427405.
STRINGi 9606.ENSP00000365596.

Chemistry

GuidetoPHARMACOLOGYi 81.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P32249.

Polymorphism databases

DMDMi 205371788.

Proteomic databases

MaxQBi P32249.
PaxDbi P32249.
PeptideAtlasi P32249.
PRIDEi P32249.

Protocols and materials databases

DNASUi 1880.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000376414 ; ENSP00000365596 ; ENSG00000169508 .
GeneIDi 1880.
KEGGi hsa:1880.
UCSCi uc001vog.3. human.

Organism-specific databases

CTDi 1880.
GeneCardsi GC13M099946.
HGNCi HGNC:3128. GPR183.
HPAi CAB033690.
HPA028847.
MIMi 605741. gene.
neXtProti NX_P32249.
PharmGKBi PA162390174.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG145860.
HOGENOMi HOG000043070.
HOVERGENi HBG101355.
InParanoidi P32249.
KOi K04305.
OMAi LVFYINT.
OrthoDBi EOG7VDXPF.
PhylomeDBi P32249.
TreeFami TF350009.

Miscellaneous databases

GeneWikii GPR183.
GenomeRNAii 1880.
NextBioi 7689.
PROi P32249.
SOURCEi Search...

Gene expression databases

Bgeei P32249.
CleanExi HS_GPR183.
Genevestigatori P32249.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors."
    Birkenbach M.P., Josefsen K., Yalamanchili R.R., Lenoir G.M., Kieff E.
    J. Virol. 67:2209-2220(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  4. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  7. "Molecular pharmacological phenotyping of EBI2. An orphan seven-transmembrane receptor with constitutive activity."
    Rosenkilde M.M., Benned-Jensen T., Andersen H., Holst P.J., Kledal T.N., Luttichau H.R., Larsen J.K., Christensen J.P., Schwartz T.W.
    J. Biol. Chem. 281:13199-13208(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Ligand modulation of the Epstein-Barr virus-induced seven-transmembrane receptor EBI2: identification of a potent and efficacious inverse agonist."
    Benned-Jensen T., Smethurst C., Holst P.J., Page K.R., Sauls H., Sivertsen B., Schwartz T.W., Blanchard A., Jepras R., Rosenkilde M.M.
    J. Biol. Chem. 286:29292-29302(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF GSK682753A AS AN INVERSE AGONIST, SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF PHE-111.
  9. Cited for: IDENTIFICATION OF OXYSTEROLS AS ENDOGENOUS LIGANDS.
  10. Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-338.

Entry informationi

Entry nameiGP183_HUMAN
AccessioniPrimary (citable) accession number: P32249
Secondary accession number(s): B2R8N5, Q53F99, Q5JUH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

GSK682753A (8-[(2E)-3-(4-chlorophenyl)prop-2-enoyl]-3-[(3,4-dichlorophenyl)methyl]-1-oxa-3,8-diazaspiro[4.5]decan-2-one), an inverse agonist, selectively inhibits the constitutive activity of GPR183 with high potency and efficacy (1 Publication).

Caution

It is uncertain whether Met-1 or Met-5 is the initiator.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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