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P32249 (GP183_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 183
Alternative name(s):
Epstein-Barr virus-induced G-protein coupled receptor 2
Short name=EBI2
Short name=EBV-induced G-protein coupled receptor 2
Gene names
Name:GPR183
Synonyms:EBI2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses By similarity. Signals constitutively through G(i)-alpha, but not through G(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2). Ref.7 Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.7 Ref.8.

Tissue specificity

Expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. Also highly expressed in lung, heart and gastrointestinal tract, and weakly expressed in the urogenital system and brain. Ref.1 Ref.7

Induction

By Epstein-Barr virus (EBV). Ref.1

Miscellaneous

GSK682753A (8-[(2E)-3-(4-chlorophenyl)prop-2-enoyl]-3-[(3,4-dichlorophenyl)methyl]-1-oxa-3,8-diazaspiro[4.5]decan-2-one), an inverse agonist, selectively inhibits the constitutive activity of GPR183 with high potency and efficacy (Ref.8).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Caution

It is uncertain whether Met-1 or Met-5 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361G-protein coupled receptor 183
PRO_0000069411

Regions

Topological domain1 – 3131Extracellular Potential
Transmembrane32 – 5726Helical; Name=1; Potential
Topological domain58 – 7720Cytoplasmic Potential
Transmembrane78 – 9518Helical; Name=2; Potential
Topological domain96 – 10510Extracellular Potential
Transmembrane106 – 12722Helical; Name=3; Potential
Topological domain128 – 14922Cytoplasmic Potential
Transmembrane150 – 16819Helical; Name=4; Potential
Topological domain169 – 19224Extracellular Potential
Transmembrane193 – 21523Helical; Name=5; Potential
Topological domain216 – 24126Cytoplasmic Potential
Transmembrane242 – 26524Helical; Name=6; Potential
Topological domain266 – 28722Extracellular Potential
Transmembrane288 – 31225Helical; Name=7; Potential
Topological domain313 – 36149Cytoplasmic Potential
Region126 – 1349Interaction with G proteins

Amino acid modifications

Modified residue3281Phosphoserine By similarity
Disulfide bond104 ↔ 181 By similarity

Natural variations

Natural variant3381A → V in an acute myeloid leukemia sample; somatic mutation. Ref.10
VAR_054147

Experimental info

Mutagenesis1111F → A: 500-fold decrease of IC(50) for GSK682753A. Ref.8
Sequence conflict61A → V in BAD97110. Ref.3
Sequence conflict1791I → N in BAG36232. Ref.2
Sequence conflict3251R → Q in BAG36232. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P32249 [UniParc].

Last modified September 2, 2008. Version 3.
Checksum: B5A2171F34C9C67B

FASTA36141,224
        10         20         30         40         50         60 
MDIQMANNFT PPSATPQGND CDLYAHHSTA RIVMPLHYSL VFIIGLVGNL LALVVIVQNR 

        70         80         90        100        110        120 
KKINSTTLYS TNLVISDILF TTALPTRIAY YAMGFDWRIG DALCRITALV FYINTYAGVN 

       130        140        150        160        170        180 
FMTCLSIDRF IAVVHPLRYN KIKRIEHAKG VCIFVWILVF AQTLPLLINP MSKQEAERIT 

       190        200        210        220        230        240 
CMEYPNFEET KSLPWILLGA CFIGYVLPLI IILICYSQIC CKLFRTAKQN PLTEKSGVNK 

       250        260        270        280        290        300 
KALNTIILII VVFVLCFTPY HVAIIQHMIK KLRFSNFLEC SQRHSFQISL HFTVCLMNFN 

       310        320        330        340        350        360 
CCMDPFIYFF ACKGYKRKVM RMLKRQVSVS ISSAVKSAPE ENSREMTETQ MMIHSKSSNG 


K 

« Hide

References

« Hide 'large scale' references
[1]"Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors."
Birkenbach M.P., Josefsen K., Yalamanchili R.R., Lenoir G.M., Kieff E.
J. Virol. 67:2209-2220(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[3]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[4]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[7]"Molecular pharmacological phenotyping of EBI2. An orphan seven-transmembrane receptor with constitutive activity."
Rosenkilde M.M., Benned-Jensen T., Andersen H., Holst P.J., Kledal T.N., Luttichau H.R., Larsen J.K., Christensen J.P., Schwartz T.W.
J. Biol. Chem. 281:13199-13208(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"Ligand modulation of the Epstein-Barr virus-induced seven-transmembrane receptor EBI2: identification of a potent and efficacious inverse agonist."
Benned-Jensen T., Smethurst C., Holst P.J., Page K.R., Sauls H., Sivertsen B., Schwartz T.W., Blanchard A., Jepras R., Rosenkilde M.M.
J. Biol. Chem. 286:29292-29302(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF GSK682753A AS AN INVERSE AGONIST, SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF PHE-111.
[9]"Oxysterols direct immune cell migration via EBI2."
Hannedouche S., Zhang J., Yi T., Shen W., Nguyen D., Pereira J.P., Guerini D., Baumgarten B.U., Roggo S., Wen B., Knochenmuss R., Noel S., Gessier F., Kelly L.M., Vanek M., Laurent S., Preuss I., Miault C. expand/collapse author list , Christen I., Karuna R., Li W., Koo D.I., Suply T., Schmedt C., Peters E.C., Falchetto R., Katopodis A., Spanka C., Roy M.O., Detheux M., Chen Y.A., Schultz P.G., Cho C.Y., Seuwen K., Cyster J.G., Sailer A.W.
Nature 475:524-527(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF OXYSTEROLS AS ENDOGENOUS LIGANDS.
[10]"DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome."
Ley T.J., Mardis E.R., Ding L., Fulton B., McLellan M.D., Chen K., Dooling D., Dunford-Shore B.H., McGrath S., Hickenbotham M., Cook L., Abbott R., Larson D.E., Koboldt D.C., Pohl C., Smith S., Hawkins A., Abbott S. expand/collapse author list , Locke D., Hillier L.W., Miner T., Fulton L., Magrini V., Wylie T., Glasscock J., Conyers J., Sander N., Shi X., Osborne J.R., Minx P., Gordon D., Chinwalla A., Zhao Y., Ries R.E., Payton J.E., Westervelt P., Tomasson M.H., Watson M., Baty J., Ivanovich J., Heath S., Shannon W.D., Nagarajan R., Walter M.J., Link D.C., Graubert T.A., DiPersio J.F., Wilson R.K.
Nature 456:66-72(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-338.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08177 mRNA. Translation: AAA35924.1.
AK292091 mRNA. Translation: BAF84780.1.
AK313443 mRNA. Translation: BAG36232.1.
AK223390 mRNA. Translation: BAD97110.1.
AL160155 Genomic DNA. Translation: CAI14308.1.
CH471085 Genomic DNA. Translation: EAX09018.1.
BC020752 mRNA. Translation: AAH20752.1.
CCDSCCDS9492.1.
PIRB45680.
RefSeqNP_004942.1. NM_004951.4.
UniGeneHs.784.

3D structure databases

ProteinModelPortalP32249.
SMRP32249. Positions 20-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108212. 2 interactions.
IntActP32249. 2 interactions.
MINTMINT-1427405.
STRING9606.ENSP00000365596.

Chemistry

GuidetoPHARMACOLOGY81.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32249.

Polymorphism databases

DMDM205371788.

Proteomic databases

MaxQBP32249.
PaxDbP32249.
PeptideAtlasP32249.
PRIDEP32249.

Protocols and materials databases

DNASU1880.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000376414; ENSP00000365596; ENSG00000169508.
GeneID1880.
KEGGhsa:1880.
UCSCuc001vog.3. human.

Organism-specific databases

CTD1880.
GeneCardsGC13M099946.
HGNCHGNC:3128. GPR183.
HPACAB033690.
HPA028847.
MIM605741. gene.
neXtProtNX_P32249.
PharmGKBPA162390174.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145860.
HOGENOMHOG000043070.
HOVERGENHBG101355.
InParanoidP32249.
KOK04305.
OMALVFYINT.
OrthoDBEOG7VDXPF.
PhylomeDBP32249.
TreeFamTF350009.

Gene expression databases

BgeeP32249.
CleanExHS_GPR183.
GenevestigatorP32249.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR183.
GenomeRNAi1880.
NextBio7689.
PROP32249.
SOURCESearch...

Entry information

Entry nameGP183_HUMAN
AccessionPrimary (citable) accession number: P32249
Secondary accession number(s): B2R8N5, Q53F99, Q5JUH7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries