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P32249

- GP183_HUMAN

UniProt

P32249 - GP183_HUMAN

Protein

G-protein coupled receptor 183

Gene

GPR183

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses By similarity. Signals constitutively through G(i)-alpha, but not through G(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2).By similarity2 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: ProtInc
    2. oxysterol binding Source: UniProtKB

    GO - Biological processi

    1. G-protein coupled receptor signaling pathway Source: UniProtKB
    2. humoral immune response Source: UniProtKB
    3. immune response Source: ProtInc
    4. mature B cell differentiation involved in immune response Source: UniProtKB
    5. positive regulation of B cell proliferation Source: UniProtKB
    6. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G-protein coupled receptor 183
    Alternative name(s):
    Epstein-Barr virus-induced G-protein coupled receptor 2
    Short name:
    EBI2
    Short name:
    EBV-induced G-protein coupled receptor 2
    Gene namesi
    Name:GPR183
    Synonyms:EBI2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:3128. GPR183.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi111 – 1111F → A: 500-fold decrease of IC(50) for GSK682753A. 1 Publication

    Organism-specific databases

    PharmGKBiPA162390174.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361G-protein coupled receptor 183PRO_0000069411Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi104 ↔ 181PROSITE-ProRule annotation
    Modified residuei328 – 3281PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiP32249.
    PaxDbiP32249.
    PeptideAtlasiP32249.
    PRIDEiP32249.

    PTM databases

    PhosphoSiteiP32249.

    Expressioni

    Tissue specificityi

    Expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. Also highly expressed in lung, heart and gastrointestinal tract, and weakly expressed in the urogenital system and brain.2 Publications

    Inductioni

    By Epstein-Barr virus (EBV).1 Publication

    Gene expression databases

    BgeeiP32249.
    CleanExiHS_GPR183.
    GenevestigatoriP32249.

    Organism-specific databases

    HPAiCAB033690.
    HPA028847.

    Interactioni

    Protein-protein interaction databases

    BioGridi108212. 2 interactions.
    IntActiP32249. 2 interactions.
    MINTiMINT-1427405.
    STRINGi9606.ENSP00000365596.

    Structurei

    3D structure databases

    ProteinModelPortaliP32249.
    SMRiP32249. Positions 20-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini58 – 7720CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini96 – 10510ExtracellularSequence Analysis
    Topological domaini128 – 14922CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini169 – 19224ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini216 – 24126CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini266 – 28722ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini313 – 36149CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5726Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei78 – 9518Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei106 – 12722Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei150 – 16819Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei193 – 21523Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei242 – 26524Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei288 – 31225Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni126 – 1349Interaction with G proteins

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145860.
    HOGENOMiHOG000043070.
    HOVERGENiHBG101355.
    InParanoidiP32249.
    KOiK04305.
    OMAiLVFYINT.
    OrthoDBiEOG7VDXPF.
    PhylomeDBiP32249.
    TreeFamiTF350009.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32249-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIQMANNFT PPSATPQGND CDLYAHHSTA RIVMPLHYSL VFIIGLVGNL    50
    LALVVIVQNR KKINSTTLYS TNLVISDILF TTALPTRIAY YAMGFDWRIG 100
    DALCRITALV FYINTYAGVN FMTCLSIDRF IAVVHPLRYN KIKRIEHAKG 150
    VCIFVWILVF AQTLPLLINP MSKQEAERIT CMEYPNFEET KSLPWILLGA 200
    CFIGYVLPLI IILICYSQIC CKLFRTAKQN PLTEKSGVNK KALNTIILII 250
    VVFVLCFTPY HVAIIQHMIK KLRFSNFLEC SQRHSFQISL HFTVCLMNFN 300
    CCMDPFIYFF ACKGYKRKVM RMLKRQVSVS ISSAVKSAPE ENSREMTETQ 350
    MMIHSKSSNG K 361
    Length:361
    Mass (Da):41,224
    Last modified:September 2, 2008 - v3
    Checksum:iB5A2171F34C9C67B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61A → V in BAD97110. 1 PublicationCurated
    Sequence conflicti179 – 1791I → N in BAG36232. (PubMed:14702039)Curated
    Sequence conflicti325 – 3251R → Q in BAG36232. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti338 – 3381A → V in an acute myeloid leukemia sample; somatic mutation. 1 Publication
    VAR_054147

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08177 mRNA. Translation: AAA35924.1.
    AK292091 mRNA. Translation: BAF84780.1.
    AK313443 mRNA. Translation: BAG36232.1.
    AK223390 mRNA. Translation: BAD97110.1.
    AL160155 Genomic DNA. Translation: CAI14308.1.
    CH471085 Genomic DNA. Translation: EAX09018.1.
    BC020752 mRNA. Translation: AAH20752.1.
    CCDSiCCDS9492.1.
    PIRiB45680.
    RefSeqiNP_004942.1. NM_004951.4.
    UniGeneiHs.784.

    Genome annotation databases

    EnsembliENST00000376414; ENSP00000365596; ENSG00000169508.
    GeneIDi1880.
    KEGGihsa:1880.
    UCSCiuc001vog.3. human.

    Polymorphism databases

    DMDMi205371788.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08177 mRNA. Translation: AAA35924.1 .
    AK292091 mRNA. Translation: BAF84780.1 .
    AK313443 mRNA. Translation: BAG36232.1 .
    AK223390 mRNA. Translation: BAD97110.1 .
    AL160155 Genomic DNA. Translation: CAI14308.1 .
    CH471085 Genomic DNA. Translation: EAX09018.1 .
    BC020752 mRNA. Translation: AAH20752.1 .
    CCDSi CCDS9492.1.
    PIRi B45680.
    RefSeqi NP_004942.1. NM_004951.4.
    UniGenei Hs.784.

    3D structure databases

    ProteinModelPortali P32249.
    SMRi P32249. Positions 20-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108212. 2 interactions.
    IntActi P32249. 2 interactions.
    MINTi MINT-1427405.
    STRINGi 9606.ENSP00000365596.

    Chemistry

    GuidetoPHARMACOLOGYi 81.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P32249.

    Polymorphism databases

    DMDMi 205371788.

    Proteomic databases

    MaxQBi P32249.
    PaxDbi P32249.
    PeptideAtlasi P32249.
    PRIDEi P32249.

    Protocols and materials databases

    DNASUi 1880.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000376414 ; ENSP00000365596 ; ENSG00000169508 .
    GeneIDi 1880.
    KEGGi hsa:1880.
    UCSCi uc001vog.3. human.

    Organism-specific databases

    CTDi 1880.
    GeneCardsi GC13M099946.
    HGNCi HGNC:3128. GPR183.
    HPAi CAB033690.
    HPA028847.
    MIMi 605741. gene.
    neXtProti NX_P32249.
    PharmGKBi PA162390174.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG145860.
    HOGENOMi HOG000043070.
    HOVERGENi HBG101355.
    InParanoidi P32249.
    KOi K04305.
    OMAi LVFYINT.
    OrthoDBi EOG7VDXPF.
    PhylomeDBi P32249.
    TreeFami TF350009.

    Miscellaneous databases

    GeneWikii GPR183.
    GenomeRNAii 1880.
    NextBioi 7689.
    PROi P32249.
    SOURCEi Search...

    Gene expression databases

    Bgeei P32249.
    CleanExi HS_GPR183.
    Genevestigatori P32249.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors."
      Birkenbach M.P., Josefsen K., Yalamanchili R.R., Lenoir G.M., Kieff E.
      J. Virol. 67:2209-2220(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Amygdala.
    3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    4. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    7. "Molecular pharmacological phenotyping of EBI2. An orphan seven-transmembrane receptor with constitutive activity."
      Rosenkilde M.M., Benned-Jensen T., Andersen H., Holst P.J., Kledal T.N., Luttichau H.R., Larsen J.K., Christensen J.P., Schwartz T.W.
      J. Biol. Chem. 281:13199-13208(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "Ligand modulation of the Epstein-Barr virus-induced seven-transmembrane receptor EBI2: identification of a potent and efficacious inverse agonist."
      Benned-Jensen T., Smethurst C., Holst P.J., Page K.R., Sauls H., Sivertsen B., Schwartz T.W., Blanchard A., Jepras R., Rosenkilde M.M.
      J. Biol. Chem. 286:29292-29302(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF GSK682753A AS AN INVERSE AGONIST, SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF PHE-111.
    9. Cited for: IDENTIFICATION OF OXYSTEROLS AS ENDOGENOUS LIGANDS.
    10. Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-338.

    Entry informationi

    Entry nameiGP183_HUMAN
    AccessioniPrimary (citable) accession number: P32249
    Secondary accession number(s): B2R8N5, Q53F99, Q5JUH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    GSK682753A (8-[(2E)-3-(4-chlorophenyl)prop-2-enoyl]-3-[(3,4-dichlorophenyl)methyl]-1-oxa-3,8-diazaspiro[4.5]decan-2-one), an inverse agonist, selectively inhibits the constitutive activity of GPR183 with high potency and efficacy.1 Publication

    Caution

    It is uncertain whether Met-1 or Met-5 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3