P32247 (BRS3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bombesin receptor subtype-3
Short name=BRS-3

Gene names

Name:

BRS3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subunit structure	Interacts with C6orf89. Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	In germ cells in testis. Lung carcinoma cells.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adult feeding behavior Traceable author statement PubMed 9367152. Source: ProtInc glucose metabolic process Traceable author statement PubMed 9367152. Source: ProtInc regulation of blood pressure Traceable author statement PubMed 9367152. Source: ProtInc
Cellular_component	integral to membrane Traceable author statement PubMed 9367152. Source: ProtInc neuron projection Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara plasma membrane Non-traceable author statement PubMed 9367152. Source: ProtInc
Molecular_function	bombesin receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Bombesin receptor subtype-3		PRO_0000069196
Regions
	Topological domain	1 – 41	41	Extracellular Potential
	Transmembrane	42 – 63	22	Helical; Name=1; Potential
	Topological domain	64 – 82	19	Cytoplasmic Potential
	Transmembrane	83 – 103	21	Helical; Name=2; Potential
	Topological domain	104 – 121	18	Extracellular Potential
	Transmembrane	122 – 143	22	Helical; Name=3; Potential
	Topological domain	144 – 163	20	Cytoplasmic Potential
	Transmembrane	164 – 184	21	Helical; Name=4; Potential
	Topological domain	185 – 220	36	Extracellular Potential
	Transmembrane	221 – 241	21	Helical; Name=5; Potential
	Topological domain	242 – 272	31	Cytoplasmic Potential
	Transmembrane	273 – 293	21	Helical; Name=6; Potential
	Topological domain	294 – 313	20	Extracellular Potential
	Transmembrane	314 – 333	20	Helical; Name=7; Potential
	Topological domain	334 – 399	66	Cytoplasmic Potential
Amino acid modifications
	Lipidation	347	1	S-palmitoyl cysteine By similarity
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Disulfide bond	120 ↔ 203		By similarity
Natural variations
	Natural variant	53	1	T → P. Corresponds to variant rs5232 [ dbSNP | Ensembl ].		VAR_011844
	Natural variant	162	1	L → Q. Corresponds to variant rs5234 [ dbSNP | Ensembl ].		VAR_011845

Sequences

	Sequence		Length	Mass (Da)
	P32247 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 979C93E962A3A28A	FASTA	399	44,411
	10 20 30 40 50 60 MAQRQPHSPN QTLISITNDT ESSSSVVSND NTNKGWSGDN SPGIEALCAI YITYAVIISV 70 80 90 100 110 120 GILGNAILIK VFFKTKSMQT VPNIFITSLA FGDLLLLLTC VPVDATHYLA EGWLFGRIGC 130 140 150 160 170 180 KVLSFIRLTS VGVSVFTLTI LSADRYKAVV KPLERQPSNA ILKTCVKAGC VWIVSMIFAL 190 200 210 220 230 240 PEAIFSNVYT FRDPNKNMTF ESCTSYPVSK KLLQEIHSLL CFLVFYIIPL SIISVYYSLI 250 260 270 280 290 300 ARTLYKSTLN IPTEEQSHAR KQIESRKRIA RTVLVLVALF ALCWLPNHLL YLYHSFTSQT 310 320 330 340 350 360 YVDPSAMHFI FTIFSRVLAF SNSCVNPFAL YWLSKSFQKH FKAQLFCCKA ERPEPPVADT 370 380 390 SLTTLAVMGT VPGTGSIQMS EISVTSFTGC SVKQAEDRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BRS-3: a novel bombesin receptor subtype selectively expressed in testis and lung carcinoma cells." Fathi Z., Corjay M.H., Shapira H., Wada E., Benya R., Jensen R., Viallet J., Sausville E.A., Battey J.F. J. Biol. Chem. 268:5979-5984(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus." Gorbulev V., Akhundova A., Grzeschik K.H., Fahrenholz F. FEBS Lett. 340:260-264(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Uterus.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Cloning of a novel protein interacting with BRS-3 and its effects in wound repair of bronchial epithelial cells." Liu H.J., Tan Y.R., Li M.L., Liu C., Xiang Y., Qin X.Q. PLoS ONE 6:E23072-E23072(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH C6ORF89.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08893 mRNA. Translation: AAA35604.1. X76498 Genomic DNA. Translation: CAA54031.1. AY585193 mRNA. Translation: AAT79496.1. Z97632 Genomic DNA. Translation: CAB10731.1.
IPI	IPI00027686.
PIR	A46632.
RefSeq	NP_001718.1. NM_001727.1.
UniGene	Hs.121484.
3D structure databases
ProteinModelPortal	P32247.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000359682.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P32247.
Polymorphism databases
DMDM	416726.
Proteomic databases
PaxDb	P32247.
PeptideAtlas	P32247.
PRIDE	P32247.
Protocols and materials databases
DNASU	680.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370648; ENSP00000359682; ENSG00000102239.
GeneID	680.
KEGG	hsa:680.
UCSC	uc004ezv.1. human.
Organism-specific databases
CTD	680.
GeneCards	GC0XP135570.
HGNC	HGNC:1113. BRS3.
HPA	HPA003626.
MIM	300107. gene.
neXtProt	NX_P32247.
PharmGKB	PA25427.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG260553.
HOGENOM	HOG000230781.
HOVERGEN	HBG004898.
InParanoid	P32247.
KO	K04170.
OMA	CAKAGCI.
OrthoDB	EOG4Z36DT.
PhylomeDB	P32247.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P32247.
CleanEx	HS_BRS3.
Genevestigator	P32247.
GermOnline	ENSG00000102239. Homo sapiens.
Family and domain databases
InterPro	IPR001560. Bombesin_rcpt_3. IPR001556. Bombsn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00637. BOMBESIN3R. PR00358. BOMBESINR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P32247.
ChEMBL	CHEMBL4080.
GenomeRNAi	680.
NextBio	2798.
SOURCE	Search...

Entry information

Entry name

BRS3_HUMAN

Accession

Primary (citable) accession number: P32247

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.