Reviewed, UniProtKB/Swiss-Prot P32241 (VIPR1_HUMAN)

Names and origin

Protein names

Recommended name:
    Vasoactive intestinal polypeptide receptor 1
      Short name=VIP-R-1
Alternative name(s):
    Pituitary adenylate cyclase-activating polypeptide type II receptor
      Short name=PACAP type II receptor
    PACAP-R-2

Gene names

Name:

VIPR1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38. Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	In lung, HT29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and Tsup-1, but not in the T-cell lines PEER, MOLT-4, HSB and YT. Ref.10
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cyclic nucleotide second messenger Ref.1 Traceable author statement. Source: ProtInc digestion Ref.1 Traceable author statement. Source: ProtInc immune response Ref.1 Traceable author statement. Source: ProtInc muscle contraction Ref.1 Traceable author statement. Source: ProtInc positive regulation of cell proliferation Traceable author statement. Source: ProtInc synaptic transmission Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	vasoactive intestinal polypeptide receptor activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Short (identifier: P32241-1)

Also known as: hIVR8;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Long (identifier: P32241-2)

Also known as: hIVR5;

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MRPPSPLPARWLCVLAGALAWALGPAGGQAAR → MPPPPLLSLR...RAARSLLGSS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 457	427	Vasoactive intestinal polypeptide receptor 1		PRO_0000012855
Regions
	Topological domain	31 – 142	112	Extracellular Potential
	Transmembrane	143 – 167	25	1 Potential
	Topological domain	168 – 174	7	Cytoplasmic Potential
	Transmembrane	175 – 194	20	2 Potential
	Topological domain	195 – 216	22	Extracellular Potential
	Transmembrane	217 – 240	24	3 Potential
	Topological domain	241 – 254	14	Cytoplasmic Potential
	Transmembrane	255 – 276	22	4 Potential
	Topological domain	277 – 292	16	Extracellular Potential
	Transmembrane	293 – 316	24	5 Potential
	Topological domain	317 – 341	25	Cytoplasmic Potential
	Transmembrane	342 – 361	20	6 Potential
	Topological domain	362 – 373	12	Extracellular Potential
	Transmembrane	374 – 393	20	7 Potential
	Topological domain	394 – 457	64	Cytoplasmic Potential
Amino acid modifications
	Modified residue	449	1	Phosphoserine By similarity
	Modified residue	455	1	Phosphoserine By similarity
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Glycosylation	290	1	N-linked (GlcNAc...) Potential
	Disulfide bond	215 ↔ 285		Ref.11
Natural variations
	Alternative sequence	1 – 32	32	MRPPS…GQAAR → MPPPPLLSLRRLGGGWSAVT RLVVAAAGARSRGGRGGSRG AGGGGRGGVARRRRLELRAA RSLLGSS in isoform Long.		VSP_002010
	Natural variant	341	1	R → M: dbSNP rs17855906. Ref.7		VAR_055041
	Natural variant	445	1	R → L: dbSNP rs3733055.		VAR_020021
Experimental info
	Sequence conflict	284	1	G → GLLR in CAA54814. Ref.2
	Sequence conflict	284	1	G → GLLR in CAA53046. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Short (hIVR8) [UniParc]. Last modified October 1, 1993. Version 1. Checksum: DAA40CF5BEC47D7D	FASTA	457	51,547
	10 20 30 40 50 60 MRPPSPLPAR WLCVLAGALA WALGPAGGQA ARLQEECDYV QMIEVQHKQC LEEAQLENET 70 80 90 100 110 120 IGCSKMWDNL TCWPATPRGQ VVVLACPLIF KLFSSIQGRN VSRSCTDEGW THLEPGPYPI 130 140 150 160 170 180 ACGLDDKAAS LDEQQTMFYG SVKTGYTIGY GLSLATLLVA TAILSLFRKL HCTRNYIHMH 190 200 210 220 230 240 LFISFILRAA AVFIKDLALF DSGESDQCSE GSVGCKAAMV FFQYCVMANF FWLLVEGLYL 250 260 270 280 290 300 YTLLAVSFFS ERKYFWGYIL IGWGVPSTFT MVWTIARIHF EDYGCWDTIN SSLWWIIKGP 310 320 330 340 350 360 ILTSILVNFI LFICIIRILL QKLRPPDIRK SDSSPYSRLA RSTLLLIPLF GVHYIMFAFF 370 380 390 400 410 420 PDNFKPEVKM VFELVVGSFQ GFVVAILYCF LNGEVQAELR RKWRRWHLQG VLGWNPKYRH 430 440 450 PSGGSNGATC STQVSMLTRV SPGARRSSSF QAEVSLV
	Isoform Long (hIVR5). Checksum: D6E529795785C3AA	FASTA	492	55,009
	10 20 30 40 50 60 MPPPPLLSLR RLGGGWSAVT RLVVAAAGAR SRGGRGGSRG AGGGGRGGVA RRRRLELRAA 70 80 90 100 110 120 RSLLGSSLQE ECDYVQMIEV QHKQCLEEAQ LENETIGCSK MWDNLTCWPA TPRGQVVVLA 130 140 150 160 170 180 CPLIFKLFSS IQGRNVSRSC TDEGWTHLEP GPYPIACGLD DKAASLDEQQ TMFYGSVKTG 190 200 210 220 230 240 YTIGYGLSLA TLLVATAILS LFRKLHCTRN YIHMHLFISF ILRAAAVFIK DLALFDSGES 250 260 270 280 290 300 DQCSEGSVGC KAAMVFFQYC VMANFFWLLV EGLYLYTLLA VSFFSERKYF WGYILIGWGV 310 320 330 340 350 360 PSTFTMVWTI ARIHFEDYGC WDTINSSLWW IIKGPILTSI LVNFILFICI IRILLQKLRP 370 380 390 400 410 420 PDIRKSDSSP YSRLARSTLL LIPLFGVHYI MFAFFPDNFK PEVKMVFELV VGSFQGFVVA 430 440 450 460 470 480 ILYCFLNGEV QAELRRKWRR WHLQGVLGWN PKYRHPSGGS NGATCSTQVS MLTRVSPGAR 490 RSSSFQAEVS LV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional expression of a human neuroendocrine vasoactive intestinal peptide receptor." Sreedharan S.P., Patel D.R., Huang J.-X., Goetzl E.J. Biochem. Biophys. Res. Commun. 193:546-553(1993) [PubMed: 8390245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT). Tissue: Intestine.
[2]	"Human intestinal VIP receptor: cloning and functional expression of two cDNA encoding proteins with different N-terminal domains." Couvineau A., Rouyer-Fessard C., Darmoul D., Maoret J.J., Carrero I., Ogier-Denis E., Laburthe M. Biochem. Biophys. Res. Commun. 200:769-776(1994) [PubMed: 8179610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SHORT AND LONG), ALTERNATIVE SPLICING. Tissue: Intestine.
[3]	"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	Martin A.L., Kaighin V.A., Aronstam R.S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Tissue: Lung.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Lung.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT), VARIANT MET-341.
[8]	"Molecular cloning and functional characterization of a human liver vasoactive intestinal peptide receptor." Gagnon A.W., Aiyar N., Elshourbagy N.A. Cell. Signal. 6:321-333(1994) [PubMed: 7917790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-457. Tissue: Liver.
[9]	"Highly conserved aspartate 68, tryptophane 73 and glycine 109 in the N-terminal extracellular domain of the human VIP receptor are essential for its ability to bind VIP." Couvineau A., Gaudin P., Maoret J.J., Rouyer-Fessard C., Nicole P., Laburthe M. Biochem. Biophys. Res. Commun. 206:246-252(1995) [PubMed: 7818527] [Abstract] Cited for: INTERACTION WITH VIP.
[10]	"Predominant expression of type II vasoactive intestinal peptide receptors by human T lymphoblastoma cells: transduction of both Ca2+ and cyclic AMP signals." Xia M., Sreedharan S.P., Goetzl E.J. J. Clin. Immunol. 16:21-30(1996) [PubMed: 8926282] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[11]	"Importance of conserved cysteines in the extracellular loops of human PACAP/VIP1 receptor for ligand binding and stimulation of cAMP production." Knudsen S.M., Tams J.W., Wulff B.S., Fahrenkrug J. Ann. N. Y. Acad. Sci. 865:259-265(1998) [PubMed: 9928020] [Abstract] Cited for: DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L13288 mRNA. Translation: AAA36805.1.
U11087 , U11079, U11080, U11081, U11083, U11084, U11085, U11086 Genomic DNA. Translation: AAB60362.1.
X75299 mRNA. Translation: CAA53046.1.
X77777 mRNA. Translation: CAA54814.1.
AB065669 Genomic DNA. Translation: BAC05895.1.
EF577396 mRNA. Translation: ABQ52416.1.
AK314334 mRNA. Translation: BAG36980.1.
CH471055 Genomic DNA. Translation: EAW64649.1.
BC064424 mRNA. Translation: AAH64424.1.
L20295 mRNA. Translation: AAA36802.1.

IPI

IPI00027680.
IPI00218266.

PIR

JC2194.
JC2195.

RefSeq

NP_004615.2.

UniGene

Hs.348500

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OF2	X-ray	2.20	C	400-408	[»]
1OGT	X-ray	1.47	C	400-408	[»]
3B3I	X-ray	1.86	C	400-408	[»]

ModBase

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Protein family/group databases

GPCRDB

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PTM databases

PhosphoSite

P32241.

Proteomic databases

PRIDE

P32241.

Genome annotation databases

Ensembl

ENSG00000114812. Homo sapiens. [Contig view]

GeneID

7433.

KEGG

hsa:7433.

Organism-specific databases

GeneCards

GC03P042505.

H-InvDB

HIX0003202.

HGNC

HGNC:12694. VIPR1.

MIM

192321. gene.

PharmGKB

PA37313.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P32241.

HOVERGEN

P32241.

OMA

P32241. MLTRVSP.

Gene expression databases

ArrayExpress

P32241.

Bgee

P32241.

CleanEx

HS_VIPR1.

GermOnline

ENSG00000114812. Homo sapiens.

Family and domain databases

InterPro

IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR001571. GPCR_2_VIP_rcpt.
IPR001771. GPCR_2_VIP_rcpt_1.
[Graphical view]

Pfam

PF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]

PRINTS

PR00249. GPCRSECRETIN.
PR00491. VASOACTVEIPR.
PR01154. VIP1RECEPTOR.

SMART

SM00008. HormR. 1 hit.
[Graphical view]

PROSITE

PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

ProtoNet

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Other Resources

NextBio

29114.

SOURCE

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Entry information

Entry name

VIPR1_HUMAN

Accession

Primary (citable) accession number: P32241
Secondary accession number(s): A5JUT9, Q15871, Q6P2M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)