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P32240

- PE2R4_MOUSE

UniProt

P32240 - PE2R4_MOUSE

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Protein

Prostaglandin E2 receptor EP4 subtype

Gene

Ptger4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

GO - Molecular functioni

  1. prostaglandin E receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
  3. bone development Source: UniProtKB
  4. cellular response to mechanical stimulus Source: UniProtKB
  5. ERK1 and ERK2 cascade Source: UniProtKB
  6. JNK cascade Source: UniProtKB
  7. negative regulation of cytokine secretion Source: UniProtKB
  8. negative regulation of eosinophil extravasation Source: UniProtKB
  9. negative regulation of inflammatory response Source: UniProtKB
  10. negative regulation of integrin activation Source: UniProtKB
  11. positive regulation of cytokine secretion Source: UniProtKB
  12. positive regulation of inflammatory response Source: UniProtKB
  13. regulation of ossification Source: MGI
  14. regulation of stress fiber assembly Source: UniProtKB
  15. response to lipopolysaccharide Source: MGI
  16. T-helper cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_230556. Prostanoid ligand receptors.
REACT_242871. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name:
PGE receptor EP4 subtype
Short name:
PGE2 receptor EP4 subtype
Alternative name(s):
Prostanoid EP4 receptor
Gene namesi
Name:Ptger4
Synonyms:Ptgerep4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:104311. Ptger4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444ExtracellularSequence AnalysisAdd
BLAST
Transmembranei45 – 6824Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini69 – 8012CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei81 – 10424Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini105 – 12117ExtracellularSequence AnalysisAdd
BLAST
Transmembranei122 – 14019Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini141 – 16020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei161 – 18525Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini186 – 20924ExtracellularSequence AnalysisAdd
BLAST
Transmembranei210 – 23627Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini237 – 29559CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei296 – 32328Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini324 – 34017ExtracellularSequence AnalysisAdd
BLAST
Transmembranei341 – 36020Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini361 – 513153CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Prostaglandin E2 receptor EP4 subtypePRO_0000070065Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 195PROSITE-ProRule annotation
Modified residuei402 – 4021PhosphoserineBy similarity
Modified residuei405 – 4051PhosphoserineBy similarity
Modified residuei407 – 4071PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP32240.

PTM databases

PhosphoSiteiP32240.

Expressioni

Tissue specificityi

Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus.

Gene expression databases

BgeeiP32240.
CleanExiMM_PTGER4.
ExpressionAtlasiP32240. baseline and differential.
GenevestigatoriP32240.

Interactioni

Subunit structurei

Interacts with FEM1A.By similarity

Structurei

3D structure databases

ProteinModelPortaliP32240.
SMRiP32240. Positions 45-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi255 – 2606Poly-Ala

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295140.
GeneTreeiENSGT00760000119188.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP32240.
KOiK04261.
OMAiINQLYQP.
OrthoDBiEOG72VH6G.
PhylomeDBiP32240.
TreeFamiTF324982.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF6. PTHR11866:SF6. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32240-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA
60 70 80 90 100
VMFIFGVVGN LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV
110 120 130 140 150
TIATYMKGQW PGDQALCDYS TFILLFFGLS GLSIICAMSI ERYLAINHAY
160 170 180 190 200
FYSHYVDKRL AGLTLFAIYA SNVLFCALPN MGLGRSERQY PGTWCFIDWT
210 220 230 240 250
TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR QFMRRTSLGT
260 270 280 290 300
EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI
310 320 330 340 350
LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP
360 370 380 390 400
ILDPWIYILL RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA
410 420 430 440 450
MSGHSRSFLA RELKEISSTS QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ
460 470 480 490 500
ADTTSLRTLR ISETSDSSQG QDSESVLLVD EVSGSHREEP ASKGNSLQVT
510
FPSETLKLSE KCI
Length:513
Mass (Da):56,158
Last modified:October 1, 1993 - v1
Checksum:iE29216087A561473
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13458 mRNA. Translation: BAA02714.1.
U70365 Genomic DNA. Translation: AAC52897.1.
CCDSiCCDS27365.1.
PIRiA46638.
RefSeqiNP_032991.1. NM_008965.2.
UniGeneiMm.18509.

Genome annotation databases

EnsembliENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
GeneIDi19219.
KEGGimmu:19219.
UCSCiuc007vcy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13458 mRNA. Translation: BAA02714.1 .
U70365 Genomic DNA. Translation: AAC52897.1 .
CCDSi CCDS27365.1.
PIRi A46638.
RefSeqi NP_032991.1. NM_008965.2.
UniGenei Mm.18509.

3D structure databases

ProteinModelPortali P32240.
SMRi P32240. Positions 45-230.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P32240.
ChEMBLi CHEMBL2489.
GuidetoPHARMACOLOGYi 343.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P32240.

Proteomic databases

PRIDEi P32240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047379 ; ENSMUSP00000048736 ; ENSMUSG00000039942 .
GeneIDi 19219.
KEGGi mmu:19219.
UCSCi uc007vcy.2. mouse.

Organism-specific databases

CTDi 5734.
MGIi MGI:104311. Ptger4.

Phylogenomic databases

eggNOGi NOG295140.
GeneTreei ENSGT00760000119188.
HOGENOMi HOG000251595.
HOVERGENi HBG053556.
InParanoidi P32240.
KOi K04261.
OMAi INQLYQP.
OrthoDBi EOG72VH6G.
PhylomeDBi P32240.
TreeFami TF324982.

Enzyme and pathway databases

Reactomei REACT_230556. Prostanoid ligand receptors.
REACT_242871. G alpha (s) signalling events.

Miscellaneous databases

NextBioi 295988.
PROi P32240.
SOURCEi Search...

Gene expression databases

Bgeei P32240.
CleanExi MM_PTGER4.
ExpressionAtlasi P32240. baseline and differential.
Genevestigatori P32240.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view ]
PANTHERi PTHR11866. PTHR11866. 1 hit.
PTHR11866:SF6. PTHR11866:SF6. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2 subtype."
    Honda A., Sugimoto Y., Namba T., Watabe A., Irie A., Negishi M., Narumiya S., Ichikawa A.
    J. Biol. Chem. 268:7759-7762(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BDF1.
  2. "Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and expression of the murine prostaglandin EP4 receptor gene."
    Arakawa T., Laneuville O., Miller C.A., Lakkides K.M., Wingerd B.A., Dewitt D.L., Smith W.L.
    J. Biol. Chem. 271:29569-29575(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-317.

Entry informationi

Entry nameiPE2R4_MOUSE
AccessioniPrimary (citable) accession number: P32240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally designated as the EP2 subtype.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3