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P32240 (PE2R4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prostaglandin E2 receptor EP4 subtype

Short name=PGE receptor EP4 subtype
Short name=PGE2 receptor EP4 subtype
Alternative name(s):
Prostanoid EP4 receptor
Gene names
Name:Ptger4
Synonyms:Ptgerep4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Subunit structure

Interacts with FEM1A By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus.

Post-translational modification

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Caution

Was originally designated as the EP2 subtype.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: UniProtKB

JNK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

T-helper cell differentiation

Inferred from direct assay PubMed 19465928. Source: UniProtKB

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Traceable author statement PubMed 10749873. Source: MGI

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

bone development

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to mechanical stimulus

Inferred from expression pattern PubMed 14665434. Source: UniProtKB

negative regulation of cytokine secretion

Inferred from mutant phenotype PubMed 11602631. Source: UniProtKB

negative regulation of eosinophil extravasation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of inflammatory response

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of integrin activation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cytokine secretion

Inferred from direct assay PubMed 19465928. Source: UniProtKB

positive regulation of inflammatory response

Inferred from direct assay PubMed 19465928. Source: UniProtKB

regulation of ossification

Inferred from mutant phenotype PubMed 10749873. Source: MGI

regulation of stress fiber assembly

Inferred from direct assay PubMed 14665434. Source: UniProtKB

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 15834430. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprostaglandin E receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Prostaglandin E2 receptor EP4 subtype
PRO_0000070065

Regions

Topological domain1 – 4444Extracellular Potential
Transmembrane45 – 6824Helical; Name=1; Potential
Topological domain69 – 8012Cytoplasmic Potential
Transmembrane81 – 10424Helical; Name=2; Potential
Topological domain105 – 12117Extracellular Potential
Transmembrane122 – 14019Helical; Name=3; Potential
Topological domain141 – 16020Cytoplasmic Potential
Transmembrane161 – 18525Helical; Name=4; Potential
Topological domain186 – 20924Extracellular Potential
Transmembrane210 – 23627Helical; Name=5; Potential
Topological domain237 – 29559Cytoplasmic Potential
Transmembrane296 – 32328Helical; Name=6; Potential
Topological domain324 – 34017Extracellular Potential
Transmembrane341 – 36020Helical; Name=7; Potential
Topological domain361 – 513153Cytoplasmic Potential
Compositional bias255 – 2606Poly-Ala

Amino acid modifications

Modified residue4021Phosphoserine By similarity
Modified residue4051Phosphoserine By similarity
Modified residue4071Phosphoserine By similarity
Glycosylation321N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 195 By similarity

Sequences

Sequence LengthMass (Da)Tools
P32240 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E29216087A561473

FASTA51356,158
        10         20         30         40         50         60 
MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA VMFIFGVVGN 

        70         80         90        100        110        120 
LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV TIATYMKGQW PGDQALCDYS 

       130        140        150        160        170        180 
TFILLFFGLS GLSIICAMSI ERYLAINHAY FYSHYVDKRL AGLTLFAIYA SNVLFCALPN 

       190        200        210        220        230        240 
MGLGRSERQY PGTWCFIDWT TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR 

       250        260        270        280        290        300 
QFMRRTSLGT EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI 

       310        320        330        340        350        360 
LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP ILDPWIYILL 

       370        380        390        400        410        420 
RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA MSGHSRSFLA RELKEISSTS 

       430        440        450        460        470        480 
QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ ADTTSLRTLR ISETSDSSQG QDSESVLLVD 

       490        500        510 
EVSGSHREEP ASKGNSLQVT FPSETLKLSE KCI 

« Hide

References

[1]"Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2 subtype."
Honda A., Sugimoto Y., Namba T., Watabe A., Irie A., Negishi M., Narumiya S., Ichikawa A.
J. Biol. Chem. 268:7759-7762(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BDF1.
[2]"Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and expression of the murine prostaglandin EP4 receptor gene."
Arakawa T., Laneuville O., Miller C.A., Lakkides K.M., Wingerd B.A., Dewitt D.L., Smith W.L.
J. Biol. Chem. 271:29569-29575(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-317.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13458 mRNA. Translation: BAA02714.1.
U70365 Genomic DNA. Translation: AAC52897.1.
PIRA46638.
RefSeqNP_032991.1. NM_008965.2.
UniGeneMm.18509.

3D structure databases

ProteinModelPortalP32240.
SMRP32240. Positions 45-230.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP32240.
ChEMBLCHEMBL2489.
GuidetoPHARMACOLOGY343.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32240.

Proteomic databases

PRIDEP32240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
GeneID19219.
KEGGmmu:19219.
UCSCuc007vcy.2. mouse.

Organism-specific databases

CTD5734.
MGIMGI:104311. Ptger4.

Phylogenomic databases

eggNOGNOG295140.
GeneTreeENSGT00690000101953.
HOGENOMHOG000251595.
HOVERGENHBG053556.
InParanoidP32240.
KOK04261.
OMAINQLYQP.
OrthoDBEOG72VH6G.
PhylomeDBP32240.
TreeFamTF324982.

Gene expression databases

ArrayExpressP32240.
BgeeP32240.
CleanExMM_PTGER4.
GenevestigatorP32240.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF6. PTHR11866:SF6. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295988.
PROP32240.
SOURCESearch...

Entry information

Entry namePE2R4_MOUSE
AccessionPrimary (citable) accession number: P32240
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries