Prostaglandin E2 receptor EP4 subtype

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P32240 (PE2R4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name=PGE receptor EP4 subtype
Short name=PGE2 receptor EP4 subtype

Alternative name(s):
Prostanoid EP4 receptor

Gene names

Name:	Ptger4
Synonyms:	Ptgerep4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Subunit structure	Interacts with FEM1A By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus.
Post-translational modification	Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally designated as the EP2 subtype.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from sequence or structural similarity. Source: UniProtKB JNK cascade Inferred from sequence or structural similarity. Source: UniProtKB T-helper cell differentiation Inferred from direct assay PubMed 19465928. Source: UniProtKB adenylate cyclase-activating G-protein coupled receptor signaling pathway Traceable author statement PubMed 10749873. Source: MGI bone development Inferred from sequence or structural similarity. Source: UniProtKB cellular response to mechanical stimulus Inferred from expression pattern PubMed 14665434. Source: UniProtKB negative regulation of cytokine secretion Inferred from mutant phenotype PubMed 11602631. Source: UniProtKB negative regulation of eosinophil extravasation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of integrin activation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cytokine secretion Inferred from direct assay PubMed 19465928. Source: UniProtKB positive regulation of inflammatory response Inferred from direct assay PubMed 19465928. Source: UniProtKB regulation of ossification Inferred from mutant phenotype PubMed 10749873. Source: MGI regulation of stress fiber assembly Inferred from direct assay PubMed 14665434. Source: UniProtKB response to lipopolysaccharide Inferred from mutant phenotype PubMed 15834430. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	prostaglandin E receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 513

513

Prostaglandin E2 receptor EP4 subtype

PRO_0000070065

Regions

Topological domain

1 – 44

Extracellular Potential

Transmembrane

45 – 68

Helical; Name=1; Potential

Topological domain

69 – 80

Cytoplasmic Potential

Transmembrane

81 – 104

Helical; Name=2; Potential

Topological domain

105 – 121

Extracellular Potential

Transmembrane

122 – 140

Helical; Name=3; Potential

Topological domain

141 – 160

Cytoplasmic Potential

Transmembrane

161 – 185

Helical; Name=4; Potential

Topological domain

186 – 209

Extracellular Potential

Transmembrane

210 – 236

Helical; Name=5; Potential

Topological domain

237 – 295

Cytoplasmic Potential

Transmembrane

296 – 323

Helical; Name=6; Potential

Topological domain

324 – 340

Extracellular Potential

Transmembrane

341 – 360

Helical; Name=7; Potential

Topological domain

361 – 513

153

Cytoplasmic Potential

Compositional bias

255 – 260

Poly-Ala

Amino acid modifications

Modified residue

402

Phosphoserine By similarity

Modified residue

405

Phosphoserine By similarity

Modified residue

407

Phosphoserine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

117 ↔ 195

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P32240 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E29216087A561473
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FASTA

513

56,158

        10         20         30         40         50         60 
MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA VMFIFGVVGN 

        70         80         90        100        110        120 
LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV TIATYMKGQW PGDQALCDYS 

       130        140        150        160        170        180 
TFILLFFGLS GLSIICAMSI ERYLAINHAY FYSHYVDKRL AGLTLFAIYA SNVLFCALPN 

       190        200        210        220        230        240 
MGLGRSERQY PGTWCFIDWT TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR 

       250        260        270        280        290        300 
QFMRRTSLGT EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI 

       310        320        330        340        350        360 
LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP ILDPWIYILL 

       370        380        390        400        410        420 
RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA MSGHSRSFLA RELKEISSTS 

       430        440        450        460        470        480 
QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ ADTTSLRTLR ISETSDSSQG QDSESVLLVD 

       490        500        510 
EVSGSHREEP ASKGNSLQVT FPSETLKLSE KCI

« Hide

References

[1]	"Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2 subtype." Honda A., Sugimoto Y., Namba T., Watabe A., Irie A., Negishi M., Narumiya S., Ichikawa A. J. Biol. Chem. 268:7759-7762(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BDF1.
[2]	"Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and expression of the murine prostaglandin EP4 receptor gene." Arakawa T., Laneuville O., Miller C.A., Lakkides K.M., Wingerd B.A., Dewitt D.L., Smith W.L. J. Biol. Chem. 271:29569-29575(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-317.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13458 mRNA. Translation: BAA02714.1. U70365 Genomic DNA. Translation: AAC52897.1.
IPI	IPI00136545.
PIR	A46638.
RefSeq	NP_032991.1. NM_008965.1.
UniGene	Mm.18509.
3D structure databases
ProteinModelPortal	P32240.
SMR	P32240. Positions 45-256.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P32240.
Proteomic databases
PRIDE	P32240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
GeneID	19219.
KEGG	mmu:19219.
UCSC	uc007vcy.2. mouse.
Organism-specific databases
CTD	5734.
MGI	MGI:104311. Ptger4.
Phylogenomic databases
eggNOG	NOG295140.
GeneTree	ENSGT00690000101953.
HOGENOM	HOG000251595.
HOVERGEN	HBG053556.
InParanoid	P32240.
KO	K04261.
OMA	HCSDSRR.
OrthoDB	EOG495ZS1.
Gene expression databases
ArrayExpress	P32240.
Bgee	P32240.
CleanEx	MM_PTGER4.
Genevestigator	P32240.
GermOnline	ENSMUSG00000039942. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001758. Prost_EP4_rcpt. IPR008365. Prostanoid_rcpt. IPR001244. Prostglndn_DP_rcpt. [Graphical view]
PANTHER	PTHR11866. PTHR11866. 1 hit. PTHR11866:SF6. PTHR11866:SF6. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00428. PROSTAGLNDNR. PR01788. PROSTANOIDR. PR00586. PRSTNOIDEP4R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P32240.
ChEMBL	CHEMBL2489.
NextBio	295988.
SOURCE	Search...

Entry information

Entry name

PE2R4_MOUSE

Accession

Primary (citable) accession number: P32240

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents