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Protein

Prostaglandin E2 receptor EP4 subtype

Gene

Ptger4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
  • bone development Source: UniProtKB
  • cellular response to mechanical stimulus Source: UniProtKB
  • cellular response to prostaglandin E stimulus Source: GO_Central
  • ERK1 and ERK2 cascade Source: UniProtKB
  • JNK cascade Source: UniProtKB
  • negative regulation of cytokine secretion Source: UniProtKB
  • negative regulation of eosinophil extravasation Source: UniProtKB
  • negative regulation of inflammatory response Source: UniProtKB
  • negative regulation of integrin activation Source: UniProtKB
  • positive regulation of cytokine secretion Source: UniProtKB
  • positive regulation of cytosolic calcium ion concentration Source: GO_Central
  • positive regulation of inflammatory response Source: UniProtKB
  • regulation of ossification Source: MGI
  • regulation of stress fiber assembly Source: UniProtKB
  • response to lipopolysaccharide Source: MGI
  • T-helper cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-391908. Prostanoid ligand receptors.
R-MMU-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name:
PGE receptor EP4 subtype
Short name:
PGE2 receptor EP4 subtype
Alternative name(s):
Prostanoid EP4 receptor
Gene namesi
Name:Ptger4
Synonyms:Ptgerep4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:104311. Ptger4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 44ExtracellularSequence analysisAdd BLAST44
Transmembranei45 – 68Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini69 – 80CytoplasmicSequence analysisAdd BLAST12
Transmembranei81 – 104Helical; Name=2Sequence analysisAdd BLAST24
Topological domaini105 – 121ExtracellularSequence analysisAdd BLAST17
Transmembranei122 – 140Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini141 – 160CytoplasmicSequence analysisAdd BLAST20
Transmembranei161 – 185Helical; Name=4Sequence analysisAdd BLAST25
Topological domaini186 – 209ExtracellularSequence analysisAdd BLAST24
Transmembranei210 – 236Helical; Name=5Sequence analysisAdd BLAST27
Topological domaini237 – 295CytoplasmicSequence analysisAdd BLAST59
Transmembranei296 – 323Helical; Name=6Sequence analysisAdd BLAST28
Topological domaini324 – 340ExtracellularSequence analysisAdd BLAST17
Transmembranei341 – 360Helical; Name=7Sequence analysisAdd BLAST20
Topological domaini361 – 513CytoplasmicSequence analysisAdd BLAST153

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2489.
GuidetoPHARMACOLOGYi343.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700651 – 513Prostaglandin E2 receptor EP4 subtypeAdd BLAST513

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi117 ↔ 195PROSITE-ProRule annotation
Modified residuei402PhosphoserineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei407PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP32240.
PeptideAtlasiP32240.
PRIDEiP32240.

PTM databases

PhosphoSitePlusiP32240.

Expressioni

Tissue specificityi

Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus.

Gene expression databases

BgeeiENSMUSG00000039942.
CleanExiMM_PTGER4.
ExpressionAtlasiP32240. baseline and differential.
GenevisibleiP32240. MM.

Interactioni

Subunit structurei

Interacts with FEM1A.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048736.

Chemistry databases

BindingDBiP32240.

Structurei

3D structure databases

ProteinModelPortaliP32240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi255 – 260Poly-Ala6

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119188.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP32240.
KOiK04261.
OMAiIDWRTNV.
OrthoDBiEOG091G060C.
PhylomeDBiP32240.
TreeFamiTF324982.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 2 hits.
PTHR11866:SF6. PTHR11866:SF6. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA
60 70 80 90 100
VMFIFGVVGN LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV
110 120 130 140 150
TIATYMKGQW PGDQALCDYS TFILLFFGLS GLSIICAMSI ERYLAINHAY
160 170 180 190 200
FYSHYVDKRL AGLTLFAIYA SNVLFCALPN MGLGRSERQY PGTWCFIDWT
210 220 230 240 250
TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR QFMRRTSLGT
260 270 280 290 300
EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI
310 320 330 340 350
LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP
360 370 380 390 400
ILDPWIYILL RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA
410 420 430 440 450
MSGHSRSFLA RELKEISSTS QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ
460 470 480 490 500
ADTTSLRTLR ISETSDSSQG QDSESVLLVD EVSGSHREEP ASKGNSLQVT
510
FPSETLKLSE KCI
Length:513
Mass (Da):56,158
Last modified:October 1, 1993 - v1
Checksum:iE29216087A561473
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13458 mRNA. Translation: BAA02714.1.
U70365 Genomic DNA. Translation: AAC52897.1.
CCDSiCCDS27365.1.
PIRiA46638.
RefSeqiNP_032991.1. NM_008965.2.
UniGeneiMm.18509.

Genome annotation databases

EnsembliENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
GeneIDi19219.
KEGGimmu:19219.
UCSCiuc007vcy.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13458 mRNA. Translation: BAA02714.1.
U70365 Genomic DNA. Translation: AAC52897.1.
CCDSiCCDS27365.1.
PIRiA46638.
RefSeqiNP_032991.1. NM_008965.2.
UniGeneiMm.18509.

3D structure databases

ProteinModelPortaliP32240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048736.

Chemistry databases

BindingDBiP32240.
ChEMBLiCHEMBL2489.
GuidetoPHARMACOLOGYi343.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiP32240.

Proteomic databases

PaxDbiP32240.
PeptideAtlasiP32240.
PRIDEiP32240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
GeneIDi19219.
KEGGimmu:19219.
UCSCiuc007vcy.3. mouse.

Organism-specific databases

CTDi5734.
MGIiMGI:104311. Ptger4.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119188.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP32240.
KOiK04261.
OMAiIDWRTNV.
OrthoDBiEOG091G060C.
PhylomeDBiP32240.
TreeFamiTF324982.

Enzyme and pathway databases

ReactomeiR-MMU-391908. Prostanoid ligand receptors.
R-MMU-418555. G alpha (s) signalling events.

Miscellaneous databases

PROiP32240.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039942.
CleanExiMM_PTGER4.
ExpressionAtlasiP32240. baseline and differential.
GenevisibleiP32240. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 2 hits.
PTHR11866:SF6. PTHR11866:SF6. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPE2R4_MOUSE
AccessioniPrimary (citable) accession number: P32240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally designated as the EP2 subtype.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.