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P32240

- PE2R4_MOUSE

UniProt

P32240 - PE2R4_MOUSE

Protein

Prostaglandin E2 receptor EP4 subtype

Gene

Ptger4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

    GO - Molecular functioni

    1. prostaglandin E receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
    2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
    3. bone development Source: UniProtKB
    4. cellular response to mechanical stimulus Source: UniProtKB
    5. ERK1 and ERK2 cascade Source: UniProtKB
    6. JNK cascade Source: UniProtKB
    7. negative regulation of cytokine secretion Source: UniProtKB
    8. negative regulation of eosinophil extravasation Source: UniProtKB
    9. negative regulation of inflammatory response Source: UniProtKB
    10. negative regulation of integrin activation Source: UniProtKB
    11. positive regulation of cytokine secretion Source: UniProtKB
    12. positive regulation of inflammatory response Source: UniProtKB
    13. regulation of ossification Source: MGI
    14. regulation of stress fiber assembly Source: UniProtKB
    15. response to lipopolysaccharide Source: MGI
    16. T-helper cell differentiation Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prostaglandin E2 receptor EP4 subtype
    Short name:
    PGE receptor EP4 subtype
    Short name:
    PGE2 receptor EP4 subtype
    Alternative name(s):
    Prostanoid EP4 receptor
    Gene namesi
    Name:Ptger4
    Synonyms:Ptgerep4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:104311. Ptger4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 513513Prostaglandin E2 receptor EP4 subtypePRO_0000070065Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi117 ↔ 195PROSITE-ProRule annotation
    Modified residuei402 – 4021PhosphoserineBy similarity
    Modified residuei405 – 4051PhosphoserineBy similarity
    Modified residuei407 – 4071PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP32240.

    PTM databases

    PhosphoSiteiP32240.

    Expressioni

    Tissue specificityi

    Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus.

    Gene expression databases

    ArrayExpressiP32240.
    BgeeiP32240.
    CleanExiMM_PTGER4.
    GenevestigatoriP32240.

    Interactioni

    Subunit structurei

    Interacts with FEM1A.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP32240.
    SMRiP32240. Positions 45-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4444ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini69 – 8012CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini105 – 12117ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini141 – 16020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini186 – 20924ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini237 – 29559CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 34017ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini361 – 513153CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei45 – 6824Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei81 – 10424Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei122 – 14019Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei161 – 18525Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei210 – 23627Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei296 – 32328Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei341 – 36020Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi255 – 2606Poly-Ala

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295140.
    GeneTreeiENSGT00690000101953.
    HOGENOMiHOG000251595.
    HOVERGENiHBG053556.
    InParanoidiP32240.
    KOiK04261.
    OMAiINQLYQP.
    OrthoDBiEOG72VH6G.
    PhylomeDBiP32240.
    TreeFamiTF324982.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001758. Prost_EP4_rcpt.
    IPR008365. Prostanoid_rcpt.
    IPR001244. Prostglndn_DP_rcpt.
    [Graphical view]
    PANTHERiPTHR11866. PTHR11866. 1 hit.
    PTHR11866:SF6. PTHR11866:SF6. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00428. PROSTAGLNDNR.
    PR01788. PROSTANOIDR.
    PR00586. PRSTNOIDEP4R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA    50
    VMFIFGVVGN LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV 100
    TIATYMKGQW PGDQALCDYS TFILLFFGLS GLSIICAMSI ERYLAINHAY 150
    FYSHYVDKRL AGLTLFAIYA SNVLFCALPN MGLGRSERQY PGTWCFIDWT 200
    TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR QFMRRTSLGT 250
    EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI 300
    LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP 350
    ILDPWIYILL RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA 400
    MSGHSRSFLA RELKEISSTS QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ 450
    ADTTSLRTLR ISETSDSSQG QDSESVLLVD EVSGSHREEP ASKGNSLQVT 500
    FPSETLKLSE KCI 513
    Length:513
    Mass (Da):56,158
    Last modified:October 1, 1993 - v1
    Checksum:iE29216087A561473
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13458 mRNA. Translation: BAA02714.1.
    U70365 Genomic DNA. Translation: AAC52897.1.
    CCDSiCCDS27365.1.
    PIRiA46638.
    RefSeqiNP_032991.1. NM_008965.2.
    UniGeneiMm.18509.

    Genome annotation databases

    EnsembliENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942.
    GeneIDi19219.
    KEGGimmu:19219.
    UCSCiuc007vcy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13458 mRNA. Translation: BAA02714.1 .
    U70365 Genomic DNA. Translation: AAC52897.1 .
    CCDSi CCDS27365.1.
    PIRi A46638.
    RefSeqi NP_032991.1. NM_008965.2.
    UniGenei Mm.18509.

    3D structure databases

    ProteinModelPortali P32240.
    SMRi P32240. Positions 45-230.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P32240.
    ChEMBLi CHEMBL2489.
    GuidetoPHARMACOLOGYi 343.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P32240.

    Proteomic databases

    PRIDEi P32240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047379 ; ENSMUSP00000048736 ; ENSMUSG00000039942 .
    GeneIDi 19219.
    KEGGi mmu:19219.
    UCSCi uc007vcy.2. mouse.

    Organism-specific databases

    CTDi 5734.
    MGIi MGI:104311. Ptger4.

    Phylogenomic databases

    eggNOGi NOG295140.
    GeneTreei ENSGT00690000101953.
    HOGENOMi HOG000251595.
    HOVERGENi HBG053556.
    InParanoidi P32240.
    KOi K04261.
    OMAi INQLYQP.
    OrthoDBi EOG72VH6G.
    PhylomeDBi P32240.
    TreeFami TF324982.

    Miscellaneous databases

    NextBioi 295988.
    PROi P32240.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P32240.
    Bgeei P32240.
    CleanExi MM_PTGER4.
    Genevestigatori P32240.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001758. Prost_EP4_rcpt.
    IPR008365. Prostanoid_rcpt.
    IPR001244. Prostglndn_DP_rcpt.
    [Graphical view ]
    PANTHERi PTHR11866. PTHR11866. 1 hit.
    PTHR11866:SF6. PTHR11866:SF6. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00428. PROSTAGLNDNR.
    PR01788. PROSTANOIDR.
    PR00586. PRSTNOIDEP4R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2 subtype."
      Honda A., Sugimoto Y., Namba T., Watabe A., Irie A., Negishi M., Narumiya S., Ichikawa A.
      J. Biol. Chem. 268:7759-7762(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BDF1.
    2. "Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and expression of the murine prostaglandin EP4 receptor gene."
      Arakawa T., Laneuville O., Miller C.A., Lakkides K.M., Wingerd B.A., Dewitt D.L., Smith W.L.
      J. Biol. Chem. 271:29569-29575(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-317.

    Entry informationi

    Entry nameiPE2R4_MOUSE
    AccessioniPrimary (citable) accession number: P32240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally designated as the EP2 subtype.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3