P32238 (CCKAR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholecystokinin receptor type A
Short name=CCK-A receptor
Short name=CCK-AR

Alternative name(s):
Cholecystokinin-1 receptor
Short name=CCK1-R

Gene names

Name:	CCKAR
Synonyms:	CCKRA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axonogenesis Inferred from electronic annotation. Source: Compara digestion Traceable author statement Ref.1. Source: ProtInc elevation of cytosolic calcium ion concentration Traceable author statement Ref.1. Source: ProtInc feeding behavior Traceable author statement Ref.1. Source: ProtInc forebrain development Inferred from electronic annotation. Source: Compara neuron migration Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled receptor signaling pathway Traceable author statement Ref.2. Source: ProtInc response to nutrient Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	cholecystokinin receptor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 428

428

Cholecystokinin receptor type A

PRO_0000069223

Regions

Topological domain

1 – 41

41

Extracellular Potential

Transmembrane

42 – 67

26

Helical; Name=1; Potential

Topological domain

68 – 77

10

Cytoplasmic Potential

Transmembrane

78 – 104

27

Helical; Name=2; Potential

Topological domain

105 – 115

11

Extracellular Potential

Transmembrane

116 – 137

22

Helical; Name=3; Potential

Topological domain

138 – 157

20

Cytoplasmic Potential

Transmembrane

158 – 178

21

Helical; Name=4; Potential

Topological domain

179 – 210

32

Extracellular Potential

Transmembrane

211 – 234

24

Helical; Name=5; Potential

Topological domain

235 – 313

79

Cytoplasmic Potential

Transmembrane

314 – 334

21

Helical; Name=6; Potential

Topological domain

335 – 349

15

Extracellular Potential

Transmembrane

350 – 373

24

Helical; Name=7; Potential

Topological domain

374 – 428

55

Cytoplasmic Potential

Amino acid modifications

Lipidation

387

1

S-palmitoyl cysteine By similarity

Glycosylation

10

1

N-linked (GlcNAc...) Potential

Glycosylation

24

1

N-linked (GlcNAc...) Potential

Glycosylation

190

1

N-linked (GlcNAc...) Potential

Disulfide bond

18 ↔ 29

By similarity

Disulfide bond

114 ↔ 196

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

428

Helix Strand Turn

Details...

Turn

5 – 11

7

Beta strand

13 – 15

3

Beta strand

21 – 25

5

Helix

39 – 44

6

Turn

330 – 334

5

Helix

335 – 339

5

Beta strand

346 – 350

5

Helix

352 – 355

4

Sequences

	Sequence		Length	Mass (Da)
	P32238 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: A6E8FABDA805E610	FASTA	428	47,841
	10 20 30 40 50 60 MDVVDSLLVN GSNITPPCEL GLENETLFCL DQPRPSKEWQ PAVQILLYSL IFLLSVLGNT 70 80 90 100 110 120 LVITVLIRNK RMRTVTNIFL LSLAVSDLML CLFCMPFNLI PNLLKDFIFG SAVCKTTTYF 130 140 150 160 170 180 MGTSVSVSTF NLVAISLERY GAICKPLQSR VWQTKSHALK VIAATWCLSF TIMTPYPIYS 190 200 210 220 230 240 NLVPFTKNNN QTANMCRFLL PNDVMQQSWH TFLLLILFLI PGIVMMVAYG LISLELYQGI 250 260 270 280 290 300 KFEASQKKSA KERKPSTTSS GKYEDSDGCY LQKTRPPRKL ELRQLSTGSS SRANRIRSNS 310 320 330 340 350 360 SAANLMAKKR VIRMLIVIVV LFFLCWMPIF SANAWRAYDT ASAERRLSGT PISFILLLSY 370 380 390 400 410 420 TSSCVNPIIY CFMNKRFRLG FMATFPCCPN PGPPGARGEV GEEEEGGTTG ASLSRFSYSH MSASVPPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional expression of the human gallbladder cholecystokinin A receptor." Ulrich C.D., Ferber I., Holicky E., Hadac E., Buell G., Miller L.J. Biochem. Biophys. Res. Commun. 193:204-211(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gall bladder.
[2]	"Molecular cloning, functional expression and chromosomal localization of the human cholecystokinin type A receptor." Wank S.A., de Weerth A., Pisegna J.R., Huppi K. Biochem. Biophys. Res. Commun. 194:811-818(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Abnormal processing of the human cholecystokinin receptor gene in association with gallstones and obesity." Miller L.J., Holicky E.L., Ulrich C.D., Wieben E.D. Gastroenterology 109:1375-1380(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Gene structure of human cholecystokinin (CCK) type-A receptor: body fat content is related to CCK type-A receptor gene promoter polymorphism." Funakoshi A., Miyasaka K., Matsumoto H., Yamamori S., Takiguchi S., Kataoka K., Takata Y., Matsusue K., Kono A., Shimokata H. FEBS Lett. 466:264-266(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Peripheral blood leukocyte.
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Stomach.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]	"Molecular complex of cholecystokinin-8 and N-terminus of the cholecystokinin A receptor by NMR spectroscopy." Pellegrini M., Mierke D.F. Biochemistry 38:14775-14783(1999) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-47 IN COMPLEX WITH CCK.
[10]	"Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin A receptor." Giragossian C., Mierke D.F. Biochemistry 40:3804-3809(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 329-357 IN COMPLEX WITH CCK.
+	Additional computationally mapped references.

Web resources

Wikipedia

Cholecystokinin receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L13605 mRNA. Translation: AAA35659.1.
L19315 mRNA. Translation: AAA02819.1.
U23430 , U23427, U23428, U23429 Genomic DNA. Translation: AAA91123.1.
D85606 Genomic DNA. Translation: BAA90879.1.
AY322549 mRNA. Translation: AAP84362.1.
AK313978 mRNA. Translation: BAG36692.1.
CH471069 Genomic DNA. Translation: EAW92850.1.
BC074987 mRNA. Translation: AAH74987.1.

IPI

IPI00027666.

PIR

JN0692.

RefSeq

NP_000721.1. NM_000730.2.

UniGene

Hs.129.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D6G	NMR	-	A	1-47	[»]
1HZN	NMR	-	A	329-357	[»]
1PB2	model	-	A	1-381	[»]

ProteinModelPortal

P32238.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-3012991.

STRING

9606.ENSP00000295589.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P32238.

Polymorphism databases

DMDM

416772.

Proteomic databases

PRIDE

P32238.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000295589; ENSP00000295589; ENSG00000163394.

GeneID

886.

KEGG

hsa:886.

UCSC

uc003gse.1. human.

Organism-specific databases

CTD

886.

GeneCards

GC04M026483.

H-InvDB

HIX0163965.

HGNC

HGNC:1570. CCKAR.

HPA

CAB005619.

MIM

118444. gene.

neXtProt

NX_P32238.

PharmGKB

PA26142.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG238659.

HOGENOM

HOG000294067.

HOVERGEN

HBG036927.

InParanoid

P32238.

KO

K04194.

OMA

YSYSHMS.

OrthoDB

EOG43N7D1.

PhylomeDB

P32238.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P32238.

CleanEx

HS_CCKAR.

Genevestigator

P32238.

GermOnline

ENSG00000163394. Homo sapiens.

Family and domain databases

Gene3D

4.10.670.10. 1 hit.

InterPro

IPR009126. Cholcskin_rcpt.
IPR000596. Cholcy_rcpt_A.
IPR015276. CholecystokininA_recpt_N.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
PF09193. CholecysA-Rec_N. 1 hit.
[Graphical view]

PRINTS

PR01822. CCYSTOKININR.
PR00524. CCYSTOKNINAR.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P32238.

ChEMBL

CHEMBL1901.

DrugBank

DB00403. Ceruletide.

EvolutionaryTrace

P32238.

GenomeRNAi

886.

NextBio

3662.

SOURCE

Search...

Entry information

Entry name

CCKAR_HUMAN

Accession

Primary (citable) accession number: P32238
Secondary accession number(s): B2R9Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 137 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.