P32234 (128UP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding protein 128up

Gene names

Name:	128up
Synonyms:	GTP-bp
ORF Names:	CG8340

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deformed (Dfd) is required to activate 1.28up in maxillary segment cells. Ref.5
Tissue specificity	Expressed in posterior-lateral epidermis of the maxillary lobe. Ref.5
Developmental stage	Expressed in embryos and adults. Ref.5
Sequence similarities	Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	GTP binding Inferred from direct assay Ref.1. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	GTP-binding protein 128up		PRO_0000205430
Regions
	Domain	76 – 164	89	G
	Nucleotide binding	71 – 78	8	GTP By similarity
	Nucleotide binding	117 – 121	5	GTP By similarity
	Nucleotide binding	248 – 251	4	GTP By similarity
Experimental info
	Sequence conflict	2	1	S → I in CAA50701. Ref.1
	Sequence conflict	34 – 35	2	KL → NV in CAA50701. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32234 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 5B38B09D0C0A92F2	FASTA	368	41,132
	10 20 30 40 50 60 MSTILEKISA IESEMARTQK NKATSAHLGL LKAKLAKLRR ELISPKGGGG GTGEAGFEVA 70 80 90 100 110 120 KTGDARVGFV GFPSVGKSTL LSNLAGVYSE VAAYEFTTLT TVPGCIKYKG AKIQLLDLPG 130 140 150 160 170 180 IIEGAKDGKG RGRQVIAVAR TCNLIFMVLD CLKPLGHKKL LEHELEGFGI RLNKKPPNIY 190 200 210 220 230 240 YKRKDKGGIN LNSMVPQSEL DTDLVKTILS EYKIHNADIT LRYDATSDDL IDVIEGNRIY 250 260 270 280 290 300 IPCIYLLNKI DQISIEELDV IYKIPHCVPI SAHHHWNFDD LLELMWEYLR LQRIYTKPKG 310 320 330 340 350 360 QLPDYNSPVV LHNERTSIED FCNKLHRSIA KEFKYALVWG SSVKHQPQKV GIEHVLNDED VVQIVKKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene upstream of DmRP128 codes for a novel GTP-binding protein of Drosophila melanogaster." Sommer K.A., Petersen G., Bautz E.K.F. Mol. Gen. Genet. 242:391-398(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Identification and characterization of a gene activated by the deformed homeoprotein." Mahaffey J.W., Jones D.F., Hickel J.A., Griswold C.M. Development 118:203-214(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71866 Genomic DNA. Translation: CAA50701.1. AE013599 Genomic DNA. Translation: AAF58591.1. AY069810 mRNA. Translation: AAL39955.1.
PIR	S42582.
RefSeq	NP_536733.1. NM_080485.5.
UniGene	Dm.7739.
3D structure databases
ProteinModelPortal	P32234.
SMR	P32234. Positions 2-367.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32234. 3 interactions.
MINT	MINT-843420.
STRING	7227.FBpp0087084.
Proteomic databases
PaxDb	P32234.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0087976; FBpp0087084; FBgn0010339.
GeneID	36288.
KEGG	dme:Dmel_CG8340.
Organism-specific databases
CTD	36288.
FlyBase	FBgn0010339. 128up.
Phylogenomic databases
eggNOG	COG1163.
GeneTree	ENSGT00550000075013.
InParanoid	P32234.
OMA	FDVARTG.
OrthoDB	EOG4W6MBJ.
PhylomeDB	P32234.
Gene expression databases
Bgee	P32234.
GermOnline	CG8340. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006074. GTP1-OBG_CS. IPR006073. GTP_binding_domain. IPR005225. Small_GTP-bd_dom. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
Pfam	PF01926. MMR_HSR1. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
PRINTS	PR00326. GTP1OBG.
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS00905. GTP1_OBG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	36288.
NextBio	797758.

Entry information

Entry name

128UP_DROME

Accession

Primary (citable) accession number: P32234
Secondary accession number(s): Q9V648

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project