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Protein

Developmentally-regulated GTP-binding protein 1

Gene

Drg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Critical regulator of cell growth under specific conditions. Implicated in differentiation and cell cycle arrest.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 788GTPPROSITE-ProRule annotation
Nucleotide bindingi117 – 1215GTPPROSITE-ProRule annotation
Nucleotide bindingi248 – 2514GTPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Developmentally-regulated GTP-binding protein 1
Short name:
DRG-1
Alternative name(s):
Neural precursor cell expressed developmentally down-regulated protein 3
Short name:
NEDD-3
Gene namesi
Name:Drg1
Synonyms:Drg, Nedd-3, Nedd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1343297. Drg1.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: The DRG1-DFRP2/ZC3H15 complex associates with polysomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 367366Developmentally-regulated GTP-binding protein 1PRO_0000205425Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei100 – 1001Phosphothreonine; by STK16By similarity

Post-translational modificationi

Polyubiquitinated; which induces proteolytic degradation and is impaired by interaction with ZC3H15.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP32233.
PaxDbiP32233.
PRIDEiP32233.

PTM databases

iPTMnetiP32233.
PhosphoSiteiP32233.

Expressioni

Tissue specificityi

Fairly high levels in liver, heart, kidney, testis and brain. Very low levels in lung, spleen, and skeletal muscle.1 Publication

Developmental stagei

Predominantly expressed in the embryo and down-regulated during development.1 Publication

Gene expression databases

BgeeiP32233.
CleanExiMM_DRG1.
ExpressionAtlasiP32233. baseline and differential.
GenevisibleiP32233. MM.

Interactioni

Subunit structurei

Interacts (via its C-terminal) with TAL10. Interacts with STK16; the interaction phosphorylates DRG1 (By similarity). Interacts with ZC3H15; the interaction forms a polysomal DRG1-DFRP1/ZC3H15 complex which provides protein stability to DRG1 possibly by blocking poly-ubiquitination.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199310. 3 interactions.
DIPiDIP-29599N.
IntActiP32233. 1 interaction.
MINTiMINT-1857949.
STRINGi10090.ENSMUSP00000020741.

Structurei

3D structure databases

ProteinModelPortaliP32233.
SMRiP32233. Positions 2-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 290226OBG-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 1615Required for interaction with STK16By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi47 – 5610Poly-Gly

Domaini

Its C-terminal domain interacts with TAL1.

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.PROSITE-ProRule annotation
Contains 1 OBG-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1487. Eukaryota.
COG1163. LUCA.
HOGENOMiHOG000112078.
HOVERGENiHBG000948.
InParanoidiP32233.
OMAiKATSFHL.
PhylomeDBiP32233.
TreeFamiTF105677.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR031167. G_OBG.
IPR031662. GTP-binding_2.
IPR006074. GTP1-OBG_CS.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF16897. MMR_HSR1_Xtn. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTLAKIAE IEAEMARTQK NKATAHHLGL LKARLAKLRR ELITPKGGGG
60 70 80 90 100
GGPGEGFDVA KTGDARIGFV GFPSVGKSTL LSNLAGVYSE VAAYEFTTLT
110 120 130 140 150
TVPGVIRYKG AKIQLLDLPG IIEGAKDGKG RGRQVIAVAR TCNLILIVLD
160 170 180 190 200
VLKPLGHKKI IENELEGFGI RLNSKPPNIG FKKKDKGGIN LTATCPQSEL
210 220 230 240 250
DAETVKSILA EYKIHNADVT LRSDATADDL IDVVEGNRVY IPCIYVLNKI
260 270 280 290 300
DQISIEELDI IYKVPHCVPI SAHHRWNFDD LLEKIWDYLK LVRIYTKPKG
310 320 330 340 350
QLPDYTSPVV LPYSRTTVED FCMKIHKNLI KEFKYALVWG LSVKHNPQKV
360
GKDHTLEDED VIQIVKK
Length:367
Mass (Da):40,512
Last modified:October 1, 1993 - v1
Checksum:i87D77767CA0E89E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10715 mRNA. Translation: BAA01555.1.
CCDSiCCDS24355.1.
PIRiJC1349.
RefSeqiNP_031905.1. NM_007879.1.
UniGeneiMm.3250.

Genome annotation databases

EnsembliENSMUST00000020741; ENSMUSP00000020741; ENSMUSG00000020457.
GeneIDi13494.
KEGGimmu:13494.
UCSCiuc007hsh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10715 mRNA. Translation: BAA01555.1.
CCDSiCCDS24355.1.
PIRiJC1349.
RefSeqiNP_031905.1. NM_007879.1.
UniGeneiMm.3250.

3D structure databases

ProteinModelPortaliP32233.
SMRiP32233. Positions 2-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199310. 3 interactions.
DIPiDIP-29599N.
IntActiP32233. 1 interaction.
MINTiMINT-1857949.
STRINGi10090.ENSMUSP00000020741.

PTM databases

iPTMnetiP32233.
PhosphoSiteiP32233.

Proteomic databases

EPDiP32233.
PaxDbiP32233.
PRIDEiP32233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020741; ENSMUSP00000020741; ENSMUSG00000020457.
GeneIDi13494.
KEGGimmu:13494.
UCSCiuc007hsh.1. mouse.

Organism-specific databases

CTDi4733.
MGIiMGI:1343297. Drg1.

Phylogenomic databases

eggNOGiKOG1487. Eukaryota.
COG1163. LUCA.
HOGENOMiHOG000112078.
HOVERGENiHBG000948.
InParanoidiP32233.
OMAiKATSFHL.
PhylomeDBiP32233.
TreeFamiTF105677.

Miscellaneous databases

ChiTaRSiDrg1. mouse.
NextBioi284017.
PROiP32233.
SOURCEiSearch...

Gene expression databases

BgeeiP32233.
CleanExiMM_DRG1.
ExpressionAtlasiP32233. baseline and differential.
GenevisibleiP32233. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR031167. G_OBG.
IPR031662. GTP-binding_2.
IPR006074. GTP1-OBG_CS.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF16897. MMR_HSR1_Xtn. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DRG: a novel developmentally regulated GTP-binding protein."
    Sazuka T., Tomooka Y., Ikawa Y., Noda M., Kumar S.
    Biochem. Biophys. Res. Commun. 189:363-370(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  3. "Identification of DRG family regulatory proteins (DFRPs): specific regulation of DRG1 and DRG2."
    Ishikawa K., Azuma S., Ikawa S., Semba K., Inoue J.
    Genes Cells 10:139-150(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZC3H15, UBIQUITINATION.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDRG1_MOUSE
AccessioniPrimary (citable) accession number: P32233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.