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P32215 (PACR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pituitary adenylate cyclase-activating polypeptide type I receptor

Short name=PACAP type I receptor
Short name=PACAP-R-1
Short name=PACAP-R1
Gene names
Name:Adcyap1r1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

Subunit structure

Interacts (via N-terminal extracellular domain) with ADCYAP1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Hypothalamus, anterior pituitary, adrenal medulla, testicular germ cells.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Biological processDifferentiation
Spermatogenesis
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
G-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of phospholipase C activity

Inferred from direct assay Ref.4. Source: RGD

cAMP-mediated signaling

Inferred from direct assay Ref.4. Source: RGD

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

development of primary female sexual characteristics

Inferred from expression pattern PubMed 17065411. Source: RGD

multicellular organismal response to stress

Inferred from expression pattern PubMed 19181454. Source: RGD

negative regulation of cell death

Inferred from direct assay PubMed 16905223. Source: RGD

positive regulation of cAMP biosynthetic process

Inferred from direct assay PubMed 16905223. Source: RGD

positive regulation of calcium ion transport into cytosol

Inferred from mutant phenotype PubMed 16401644. Source: RGD

positive regulation of inositol phosphate biosynthetic process

Inferred from direct assay PubMed 8943280. Source: RGD

response to drug

Inferred from expression pattern PubMed 18592413. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 15976009. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 16820708. Source: RGD

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi membrane

Traceable author statement. Source: Reactome

caveola

Inferred from direct assay PubMed 17680996. Source: RGD

cell surface

Inferred from direct assay PubMed 15968088. Source: RGD

cytoplasm

Inferred from direct assay PubMed 16876955. Source: RGD

endosome

Inferred from direct assay PubMed 19416476. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from direct assay PubMed 16876955. Source: RGD

rough endoplasmic reticulum

Inferred from direct assay PubMed 9464636. Source: RGD

tight junction

Inferred from direct assay PubMed 16626868. Source: RGD

   Molecular_functionADP-ribosylation factor binding

Inferred from direct assay PubMed 11353814. Source: RGD

adenylate cyclase binding

Inferred from direct assay PubMed 17680996. Source: RGD

neuropeptide binding

Inferred from direct assay PubMed 16905223. Source: RGD

vasoactive intestinal polypeptide receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform HIP-HOP1 (identifier: P32215-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform HOP1 (identifier: P32215-2)

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: L → F
     349-376: Missing.
Isoform HOP2 (identifier: P32215-3)

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: L → F
     349-377: Missing.
Isoform HIP (identifier: P32215-4)

The sequence of this isoform differs from the canonical sequence as follows:
     377-404: Missing.
Isoform PACAP-R (identifier: P32215-5)

The sequence of this isoform differs from the canonical sequence as follows:
     349-404: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 523504Pituitary adenylate cyclase-activating polypeptide type I receptor
PRO_0000012843

Regions

Topological domain20 – 154135Extracellular Potential
Transmembrane155 – 17723Helical; Name=1; Potential
Topological domain178 – 1858Cytoplasmic Potential
Transmembrane186 – 20419Helical; Name=2; Potential
Topological domain205 – 22622Extracellular Potential
Transmembrane227 – 25226Helical; Name=3; Potential
Topological domain253 – 26715Cytoplasmic Potential
Transmembrane268 – 29023Helical; Name=4; Potential
Topological domain291 – 30818Extracellular Potential
Transmembrane309 – 33123Helical; Name=5; Potential
Topological domain332 – 40574Cytoplasmic Potential
Transmembrane406 – 42621Helical; Name=6; Potential
Topological domain427 – 44014Extracellular Potential
Transmembrane441 – 46020Helical; Name=7; Potential
Topological domain461 – 52363Cytoplasmic Potential
Region124 – 13815Important for ligand binding and specificity By similarity

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation1161N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 62 By similarity
Disulfide bond53 ↔ 117 By similarity
Disulfide bond76 ↔ 133 By similarity

Natural variations

Alternative sequence3481L → F in isoform HOP1 and isoform HOP2.
VSP_002005
Alternative sequence349 – 40456Missing in isoform PACAP-R.
VSP_002009
Alternative sequence349 – 37729Missing in isoform HOP2.
VSP_002007
Alternative sequence349 – 37628Missing in isoform HOP1.
VSP_002006
Alternative sequence377 – 40428Missing in isoform HIP.
VSP_002008

Experimental info

Sequence conflict4491F → L in AAA41792. Ref.1
Sequence conflict510 – 5112QL → HV in AAA02990. Ref.6
Sequence conflict510 – 5112QL → HV in CAA80429. Ref.6
Sequence conflict515 – 5162SL → TV in AAA02990. Ref.6
Sequence conflict515 – 5162SL → TV in CAA80429. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform HIP-HOP1 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 038CA413EED37E44

FASTA52359,637
        10         20         30         40         50         60 
MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS PGCPGMWDNI 

        70         80         90        100        110        120 
TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA DSNSLEITDM GVVGRNCTED 

       130        140        150        160        170        180 
GWSEPFPHYF DACGFDDYEP ESGDQDYYYL SVKALYTVGY STSLATLTTA MVILCRFRKL 

       190        200        210        220        230        240 
HCTRNFIHMN LFVSFMLRAI SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY 

       250        260        270        280        290        300 
FWLFIEGLYL FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 

       310        320        330        340        350        360 
STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLTN LRLRVPKKTR 

       370        380        390        400        410        420 
EDPLPVPSDQ HSPPFLSCVQ KCYCKPQRAQ QHSCKMSELS TITLRLARST LLLIPLFGIH 

       430        440        450        460        470        480 
YTVFAFSPEN VSKRERLVFE LGLGSFQGFV VAVLYCFLNG EVQAEIKRKW RSWKVNRYFT 

       490        500        510        520 
MDFKHRHPSL ASSGVNGGTQ LSILSKSSSQ LRMSSLPADN LAT 

« Hide

Isoform HOP1 [UniParc].

Checksum: CB6A851A3C426176
Show »

FASTA49556,450
Isoform HOP2 [UniParc].

Checksum: C1C969B5075952BD
Show »

FASTA49456,363
Isoform HIP [UniParc].

Checksum: 971EBE6AE9901570
Show »

FASTA49556,453
Isoform PACAP-R [UniParc].

Checksum: 9F6180156064CFC6
Show »

FASTA46753,233

References

[1]"Molecular cloning and functional expression of the pituitary adenylate cyclase-activating polypeptide type I receptor."
Wank S.A., Pisegna J.R.
Proc. Natl. Acad. Sci. U.S.A. 90:6345-6349(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreas.
[2]"Molecular cloning and functional expression of rat cDNAs encoding the receptor for pituitary adenylate cyclase activating polypeptide (PACAP)."
Hosoya M., Onda H., Ogi K., Masuda Y., Miyamoto Y., Ohtaki T., Okazaki H., Arimura A., Fujino M.
Biochem. Biophys. Res. Commun. 194:133-143(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide."
Hashimoto H., Ishihara T., Shigemoto R., Mori K., Nagata S.
Neuron 11:333-342(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[4]"Differential signal transduction by five splice variants of the PACAP receptor."
Spengler D., Waeber C., Pantaloni C., Holsboer F., Bockaert J., Seeburg P.H., Journot L.
Nature 365:170-175(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
[5]"Molecular cloning and expression of a cDNA encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP)."
Morrow J.A., Lutz E.M., West K.M., Fink G., Harmar A.J.
FEBS Lett. 329:99-105(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Olfactory bulb.
[6]Svoboda M., Ciccarelli E., Tastenoy M., Christophe J.
Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-523.
Strain: Wistar.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16465 mRNA. Translation: BAA03932.1.
L16680 mRNA. Translation: AAA41792.1.
D14908 mRNA. Translation: BAA03608.1.
D14909 mRNA. Translation: BAA03609.1.
Z23272 mRNA. Translation: CAA80810.1.
Z23273 mRNA. Translation: CAA80811.1.
Z23274 mRNA. Translation: CAA80812.1.
Z23275 mRNA. Translation: CAA80813.1.
Z23279 mRNA. Translation: CAA80817.1.
Z23282 mRNA. Translation: CAA80821.1.
Z23282 mRNA. Translation: CAA80820.1.
L16506 mRNA. Translation: AAA02990.1.
Z22735 mRNA. Translation: CAA80429.1.
PIRJN0616.
S33449.
S36114.
S39060.
S39061.
RefSeqNP_001257508.1. NM_001270579.1.
NP_001257509.1. NM_001270580.1.
NP_001257510.1. NM_001270581.1.
NP_001257511.1. NM_001270582.1.
NP_001257512.1. NM_001270583.1.
NP_598195.1. NM_133511.2.
UniGeneRn.234543.
Rn.7552.

3D structure databases

ProteinModelPortalP32215.
SMRP32215. Positions 21-142.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000016175.

Chemistry

ChEMBLCHEMBL1075233.
GuidetoPHARMACOLOGY370.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32215.

Proteomic databases

PRIDEP32215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. [P32215-1]
ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. [P32215-2]
ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. [P32215-5]
ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098. [P32215-4]
GeneID24167.
KEGGrno:24167.
UCSCRGD:2038. rat. [P32215-1]

Organism-specific databases

CTD117.
RGD2038. Adcyap1r1.

Phylogenomic databases

eggNOGNOG330024.
GeneTreeENSGT00620000087538.
HOGENOMHOG000008249.
HOVERGENHBG008318.
InParanoidP32215.
KOK04587.
OMAKWRSWKV.
OrthoDBEOG7TF78W.
PhylomeDBP32215.
TreeFamTF315710.

Gene expression databases

GenevestigatorP32215.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002285. GPCR_2_PACAP_1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PANTHERPTHR12011:SF30. PTHR12011:SF30. 1 hit.
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR01156. PACAPRECEPTR.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602477.
PROP32215.

Entry information

Entry namePACR_RAT
AccessionPrimary (citable) accession number: P32215
Secondary accession number(s): Q63414
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries