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Protein

Pituitary adenylate cyclase-activating polypeptide type I receptor

Gene

Adcyap1r1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

GO - Molecular functioni

  1. adenylate cyclase binding Source: RGD
  2. ADP-ribosylation factor binding Source: RGD
  3. neuropeptide binding Source: RGD
  4. vasoactive intestinal polypeptide receptor activity Source: InterPro

GO - Biological processi

  1. activation of phospholipase C activity Source: RGD
  2. cAMP-mediated signaling Source: RGD
  3. cell differentiation Source: UniProtKB-KW
  4. development of primary female sexual characteristics Source: RGD
  5. multicellular organismal response to stress Source: RGD
  6. negative regulation of cell death Source: RGD
  7. positive regulation of calcium ion transport into cytosol Source: RGD
  8. positive regulation of cAMP biosynthetic process Source: RGD
  9. positive regulation of inositol phosphate biosynthetic process Source: RGD
  10. response to drug Source: RGD
  11. response to estradiol Source: RGD
  12. response to ethanol Source: RGD
  13. spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Differentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_326473. Glucagon-type ligand receptors.
REACT_350908. NGF-independant TRKA activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary adenylate cyclase-activating polypeptide type I receptor
Short name:
PACAP type I receptor
Short name:
PACAP-R-1
Short name:
PACAP-R1
Gene namesi
Name:Adcyap1r1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2038. Adcyap1r1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 154135ExtracellularSequence AnalysisAdd
BLAST
Transmembranei155 – 17723Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini178 – 1858CytoplasmicSequence Analysis
Transmembranei186 – 20419Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini205 – 22622ExtracellularSequence AnalysisAdd
BLAST
Transmembranei227 – 25226Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini253 – 26715CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei268 – 29023Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini291 – 30818ExtracellularSequence AnalysisAdd
BLAST
Transmembranei309 – 33123Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini332 – 40574CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei406 – 42621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini427 – 44014ExtracellularSequence AnalysisAdd
BLAST
Transmembranei441 – 46020Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini461 – 52363CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. caveola Source: RGD
  2. cell surface Source: RGD
  3. cytoplasm Source: RGD
  4. endosome Source: RGD
  5. Golgi membrane Source: Reactome
  6. integral component of membrane Source: UniProtKB-KW
  7. neuron projection Source: RGD
  8. receptor complex Source: Ensembl
  9. rough endoplasmic reticulum Source: RGD
  10. tight junction Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 523504Pituitary adenylate cyclase-activating polypeptide type I receptorPRO_0000012843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 62By similarity
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi53 ↔ 117By similarity
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi76 ↔ 133By similarity
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP32215.

PTM databases

PhosphoSiteiP32215.

Expressioni

Tissue specificityi

Hypothalamus, anterior pituitary, adrenal medulla, testicular germ cells.

Gene expression databases

GenevestigatoriP32215.

Interactioni

Subunit structurei

Interacts (via N-terminal extracellular domain) with ADCYAP1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016175.

Structurei

3D structure databases

ProteinModelPortaliP32215.
SMRiP32215. Positions 21-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 13815Important for ligand binding and specificityBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330024.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiP32215.
KOiK04587.
OMAiRYFAVDF.
OrthoDBiEOG7TF78W.
PhylomeDBiP32215.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002285. GPCR_2_PACAP_1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01156. PACAPRECEPTR.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HIP-HOP1 (identifier: P32215-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS
60 70 80 90 100
PGCPGMWDNI TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA
110 120 130 140 150
DSNSLEITDM GVVGRNCTED GWSEPFPHYF DACGFDDYEP ESGDQDYYYL
160 170 180 190 200
SVKALYTVGY STSLATLTTA MVILCRFRKL HCTRNFIHMN LFVSFMLRAI
210 220 230 240 250
SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY FWLFIEGLYL
260 270 280 290 300
FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND
310 320 330 340 350
STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLTN
360 370 380 390 400
LRLRVPKKTR EDPLPVPSDQ HSPPFLSCVQ KCYCKPQRAQ QHSCKMSELS
410 420 430 440 450
TITLRLARST LLLIPLFGIH YTVFAFSPEN VSKRERLVFE LGLGSFQGFV
460 470 480 490 500
VAVLYCFLNG EVQAEIKRKW RSWKVNRYFT MDFKHRHPSL ASSGVNGGTQ
510 520
LSILSKSSSQ LRMSSLPADN LAT
Length:523
Mass (Da):59,637
Last modified:February 1, 1994 - v2
Checksum:i038CA413EED37E44
GO
Isoform HOP1 (identifier: P32215-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: L → F
     349-376: Missing.

Show »
Length:495
Mass (Da):56,450
Checksum:iCB6A851A3C426176
GO
Isoform HOP2 (identifier: P32215-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: L → F
     349-377: Missing.

Show »
Length:494
Mass (Da):56,363
Checksum:iC1C969B5075952BD
GO
Isoform HIP (identifier: P32215-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     377-404: Missing.

Show »
Length:495
Mass (Da):56,453
Checksum:i971EBE6AE9901570
GO
Isoform PACAP-R (identifier: P32215-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     349-404: Missing.

Show »
Length:467
Mass (Da):53,233
Checksum:i9F6180156064CFC6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti449 – 4491F → L in AAA41792 (PubMed:8392197).Curated
Sequence conflicti510 – 5112QL → HV in AAA02990 (Ref. 6) Curated
Sequence conflicti510 – 5112QL → HV in CAA80429 (Ref. 6) Curated
Sequence conflicti515 – 5162SL → TV in AAA02990 (Ref. 6) Curated
Sequence conflicti515 – 5162SL → TV in CAA80429 (Ref. 6) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei348 – 3481L → F in isoform HOP1 and isoform HOP2. CuratedVSP_002005
Alternative sequencei349 – 40456Missing in isoform PACAP-R. CuratedVSP_002009Add
BLAST
Alternative sequencei349 – 37729Missing in isoform HOP2. CuratedVSP_002007Add
BLAST
Alternative sequencei349 – 37628Missing in isoform HOP1. CuratedVSP_002006Add
BLAST
Alternative sequencei377 – 40428Missing in isoform HIP. CuratedVSP_002008Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16465 mRNA. Translation: BAA03932.1.
L16680 mRNA. Translation: AAA41792.1.
D14908 mRNA. Translation: BAA03608.1.
D14909 mRNA. Translation: BAA03609.1.
Z23272 mRNA. Translation: CAA80810.1.
Z23273 mRNA. Translation: CAA80811.1.
Z23274 mRNA. Translation: CAA80812.1.
Z23275 mRNA. Translation: CAA80813.1.
Z23279 mRNA. Translation: CAA80817.1.
Z23282 mRNA. Translation: CAA80821.1.
Z23282 mRNA. Translation: CAA80820.1.
L16506 mRNA. Translation: AAA02990.1.
Z22735 mRNA. Translation: CAA80429.1.
PIRiJN0616.
S33449.
S36114.
S39060.
S39061.
RefSeqiNP_001257508.1. NM_001270579.1. [P32215-1]
NP_001257509.1. NM_001270580.1. [P32215-4]
NP_001257510.1. NM_001270581.1. [P32215-5]
NP_001257511.1. NM_001270582.1. [P32215-5]
NP_001257512.1. NM_001270583.1. [P32215-3]
NP_598195.1. NM_133511.2. [P32215-2]
XP_008761092.1. XM_008762870.1. [P32215-3]
UniGeneiRn.234543.
Rn.7552.

Genome annotation databases

EnsembliENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. [P32215-1]
ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. [P32215-2]
ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. [P32215-5]
ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098. [P32215-4]
GeneIDi24167.
KEGGirno:24167.
UCSCiRGD:2038. rat. [P32215-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16465 mRNA. Translation: BAA03932.1.
L16680 mRNA. Translation: AAA41792.1.
D14908 mRNA. Translation: BAA03608.1.
D14909 mRNA. Translation: BAA03609.1.
Z23272 mRNA. Translation: CAA80810.1.
Z23273 mRNA. Translation: CAA80811.1.
Z23274 mRNA. Translation: CAA80812.1.
Z23275 mRNA. Translation: CAA80813.1.
Z23279 mRNA. Translation: CAA80817.1.
Z23282 mRNA. Translation: CAA80821.1.
Z23282 mRNA. Translation: CAA80820.1.
L16506 mRNA. Translation: AAA02990.1.
Z22735 mRNA. Translation: CAA80429.1.
PIRiJN0616.
S33449.
S36114.
S39060.
S39061.
RefSeqiNP_001257508.1. NM_001270579.1. [P32215-1]
NP_001257509.1. NM_001270580.1. [P32215-4]
NP_001257510.1. NM_001270581.1. [P32215-5]
NP_001257511.1. NM_001270582.1. [P32215-5]
NP_001257512.1. NM_001270583.1. [P32215-3]
NP_598195.1. NM_133511.2. [P32215-2]
XP_008761092.1. XM_008762870.1. [P32215-3]
UniGeneiRn.234543.
Rn.7552.

3D structure databases

ProteinModelPortaliP32215.
SMRiP32215. Positions 21-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016175.

Chemistry

ChEMBLiCHEMBL1075233.
GuidetoPHARMACOLOGYi370.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP32215.

Proteomic databases

PRIDEiP32215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. [P32215-1]
ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. [P32215-2]
ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. [P32215-5]
ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098. [P32215-4]
GeneIDi24167.
KEGGirno:24167.
UCSCiRGD:2038. rat. [P32215-1]

Organism-specific databases

CTDi117.
RGDi2038. Adcyap1r1.

Phylogenomic databases

eggNOGiNOG330024.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiP32215.
KOiK04587.
OMAiRYFAVDF.
OrthoDBiEOG7TF78W.
PhylomeDBiP32215.
TreeFamiTF315710.

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_326473. Glucagon-type ligand receptors.
REACT_350908. NGF-independant TRKA activation.

Miscellaneous databases

NextBioi602477.
PROiP32215.

Gene expression databases

GenevestigatoriP32215.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002285. GPCR_2_PACAP_1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01156. PACAPRECEPTR.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and functional expression of the pituitary adenylate cyclase-activating polypeptide type I receptor."
    Wank S.A., Pisegna J.R.
    Proc. Natl. Acad. Sci. U.S.A. 90:6345-6349(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "Molecular cloning and functional expression of rat cDNAs encoding the receptor for pituitary adenylate cyclase activating polypeptide (PACAP)."
    Hosoya M., Onda H., Ogi K., Masuda Y., Miyamoto Y., Ohtaki T., Okazaki H., Arimura A., Fujino M.
    Biochem. Biophys. Res. Commun. 194:133-143(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide."
    Hashimoto H., Ishihara T., Shigemoto R., Mori K., Nagata S.
    Neuron 11:333-342(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  4. "Differential signal transduction by five splice variants of the PACAP receptor."
    Spengler D., Waeber C., Pantaloni C., Holsboer F., Bockaert J., Seeburg P.H., Journot L.
    Nature 365:170-175(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
  5. "Molecular cloning and expression of a cDNA encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP)."
    Morrow J.A., Lutz E.M., West K.M., Fink G., Harmar A.J.
    FEBS Lett. 329:99-105(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Olfactory bulb.
  6. Svoboda M., Ciccarelli E., Tastenoy M., Christophe J.
    Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-523.
    Strain: Wistar.

Entry informationi

Entry nameiPACR_RAT
AccessioniPrimary (citable) accession number: P32215
Secondary accession number(s): Q63414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: April 1, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.