Pituitary adenylate cyclase-activating polypeptide type I receptor precursor

Preferred order of sections

Names and origin

Protein names

Recommended name:
Pituitary adenylate cyclase-activating polypeptide type I receptor
Short name=PACAP type I receptor
Short name=PACAP-R-1
Short name=PACAP-R1

Gene names

Name:

Adcyap1r1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.
Subunit structure	Interacts (via N-terminal extracellular domain) with ADCYAP1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Hypothalamus, anterior pituitary, adrenal medulla, testicular germ cells.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase C activity Inferred from direct assay Ref.4. Source: RGD cAMP-mediated signaling Inferred from direct assay Ref.4. Source: RGD cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW development of primary female sexual characteristics Inferred from expression pattern PubMed 17065411. Source: RGD multicellular organismal response to stress Inferred from expression pattern PubMed 19181454. Source: RGD negative regulation of cell death Inferred from direct assay PubMed 16905223. Source: RGD positive regulation of cAMP biosynthetic process Inferred from direct assay PubMed 16905223. Source: RGD positive regulation of calcium ion transport into cytosol Inferred from mutant phenotype PubMed 16401644. Source: RGD positive regulation of inositol phosphate biosynthetic process Inferred from direct assay PubMed 8943280. Source: RGD response to drug Inferred from expression pattern PubMed 18592413. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 15976009. Source: RGD response to ethanol Inferred from expression pattern PubMed 16820708. Source: RGD spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome caveola Inferred from direct assay PubMed 17680996. Source: RGD cell surface Inferred from direct assay PubMed 15968088. Source: RGD endosome Inferred from direct assay PubMed 19416476. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from direct assay PubMed 16876955. Source: RGD rough endoplasmic reticulum Inferred from direct assay PubMed 9464636. Source: RGD tight junction Inferred from direct assay PubMed 16626868. Source: RGD
Molecular_function	ADP-ribosylation factor binding Inferred from direct assay PubMed 11353814. Source: RGD adenylate cyclase binding Inferred from direct assay PubMed 17680996. Source: RGD neuropeptide binding Inferred from direct assay PubMed 16905223. Source: RGD vasoactive intestinal polypeptide receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform HIP-HOP1 (identifier: P32215-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform HOP1 (identifier: P32215-2) The sequence of this isoform differs from the canonical sequence as follows: 348-348: L → F 349-376: Missing.

Isoform HOP2 (identifier: P32215-3) The sequence of this isoform differs from the canonical sequence as follows: 348-348: L → F 349-377: Missing.

Isoform HIP (identifier: P32215-4) The sequence of this isoform differs from the canonical sequence as follows: 377-404: Missing.

Isoform PACAP-R (identifier: P32215-5) The sequence of this isoform differs from the canonical sequence as follows: 349-404: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 523

504

Pituitary adenylate cyclase-activating polypeptide type I receptor

PRO_0000012843

Regions

Topological domain

20 – 154

135

Extracellular Potential

Transmembrane

155 – 177

23

Helical; Name=1; Potential

Topological domain

178 – 185

8

Cytoplasmic Potential

Transmembrane

186 – 204

19

Helical; Name=2; Potential

Topological domain

205 – 226

22

Extracellular Potential

Transmembrane

227 – 252

26

Helical; Name=3; Potential

Topological domain

253 – 267

15

Cytoplasmic Potential

Transmembrane

268 – 290

23

Helical; Name=4; Potential

Topological domain

291 – 308

18

Extracellular Potential

Transmembrane

309 – 331

23

Helical; Name=5; Potential

Topological domain

332 – 405

74

Cytoplasmic Potential

Transmembrane

406 – 426

21

Helical; Name=6; Potential

Topological domain

427 – 440

14

Extracellular Potential

Transmembrane

441 – 460

20

Helical; Name=7; Potential

Topological domain

461 – 523

63

Cytoplasmic Potential

Region

124 – 138

15

Important for ligand binding and specificity By similarity

Amino acid modifications

Glycosylation

47

1

N-linked (GlcNAc...) Potential

Glycosylation

59

1

N-linked (GlcNAc...) Potential

Glycosylation

116

1

N-linked (GlcNAc...) Potential

Disulfide bond

33 ↔ 62

By similarity

Disulfide bond

53 ↔ 117

By similarity

Disulfide bond

76 ↔ 133

By similarity

Natural variations

Alternative sequence

348

1

L → F in isoform HOP1 and isoform HOP2.

VSP_002005

Alternative sequence

349 – 404

56

Missing in isoform PACAP-R.

VSP_002009

Alternative sequence

349 – 377

29

Missing in isoform HOP2.

VSP_002007

Alternative sequence

349 – 376

28

Missing in isoform HOP1.

VSP_002006

Alternative sequence

377 – 404

28

Missing in isoform HIP.

VSP_002008

Experimental info

Sequence conflict

449

1

F → L in AAA41792. Ref.1

Sequence conflict

510 – 511

2

QL → HV in AAA02990. Ref.6

Sequence conflict

510 – 511

2

QL → HV in CAA80429. Ref.6

Sequence conflict

515 – 516

2

SL → TV in AAA02990. Ref.6

Sequence conflict

515 – 516

2

SL → TV in CAA80429. Ref.6

Sequences

Sequence		Length	Mass (Da)
Isoform HIP-HOP1 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 038CA413EED37E44 Show »	FASTA	523	59,637
10 20 30 40 50 60 MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS PGCPGMWDNI 70 80 90 100 110 120 TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA DSNSLEITDM GVVGRNCTED 130 140 150 160 170 180 GWSEPFPHYF DACGFDDYEP ESGDQDYYYL SVKALYTVGY STSLATLTTA MVILCRFRKL 190 200 210 220 230 240 HCTRNFIHMN LFVSFMLRAI SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY 250 260 270 280 290 300 FWLFIEGLYL FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 310 320 330 340 350 360 STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLTN LRLRVPKKTR 370 380 390 400 410 420 EDPLPVPSDQ HSPPFLSCVQ KCYCKPQRAQ QHSCKMSELS TITLRLARST LLLIPLFGIH 430 440 450 460 470 480 YTVFAFSPEN VSKRERLVFE LGLGSFQGFV VAVLYCFLNG EVQAEIKRKW RSWKVNRYFT 490 500 510 520 MDFKHRHPSL ASSGVNGGTQ LSILSKSSSQ LRMSSLPADN LAT « Hide
Isoform HOP1 [UniParc]. Checksum: CB6A851A3C426176 Show »	FASTA	495	56,450
10 20 30 40 50 60 MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS PGCPGMWDNI 70 80 90 100 110 120 TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA DSNSLEITDM GVVGRNCTED 130 140 150 160 170 180 GWSEPFPHYF DACGFDDYEP ESGDQDYYYL SVKALYTVGY STSLATLTTA MVILCRFRKL 190 200 210 220 230 240 HCTRNFIHMN LFVSFMLRAI SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY 250 260 270 280 290 300 FWLFIEGLYL FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 310 320 330 340 350 360 STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYFSC VQKCYCKPQR 370 380 390 400 410 420 AQQHSCKMSE LSTITLRLAR STLLLIPLFG IHYTVFAFSP ENVSKRERLV FELGLGSFQG 430 440 450 460 470 480 FVVAVLYCFL NGEVQAEIKR KWRSWKVNRY FTMDFKHRHP SLASSGVNGG TQLSILSKSS 490 SQLRMSSLPA DNLAT « Hide
Isoform HOP2 [UniParc]. Checksum: C1C969B5075952BD Show »	FASTA	494	56,363
10 20 30 40 50 60 MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS PGCPGMWDNI 70 80 90 100 110 120 TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA DSNSLEITDM GVVGRNCTED 130 140 150 160 170 180 GWSEPFPHYF DACGFDDYEP ESGDQDYYYL SVKALYTVGY STSLATLTTA MVILCRFRKL 190 200 210 220 230 240 HCTRNFIHMN LFVSFMLRAI SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY 250 260 270 280 290 300 FWLFIEGLYL FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 310 320 330 340 350 360 STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYFCV QKCYCKPQRA 370 380 390 400 410 420 QQHSCKMSEL STITLRLARS TLLLIPLFGI HYTVFAFSPE NVSKRERLVF ELGLGSFQGF 430 440 450 460 470 480 VVAVLYCFLN GEVQAEIKRK WRSWKVNRYF TMDFKHRHPS LASSGVNGGT QLSILSKSSS 490 QLRMSSLPAD NLAT « Hide
Isoform HIP [UniParc]. Checksum: 971EBE6AE9901570 Show »	FASTA	495	56,453
10 20 30 40 50 60 MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS PGCPGMWDNI 70 80 90 100 110 120 TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA DSNSLEITDM GVVGRNCTED 130 140 150 160 170 180 GWSEPFPHYF DACGFDDYEP ESGDQDYYYL SVKALYTVGY STSLATLTTA MVILCRFRKL 190 200 210 220 230 240 HCTRNFIHMN LFVSFMLRAI SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY 250 260 270 280 290 300 FWLFIEGLYL FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 310 320 330 340 350 360 STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLTN LRLRVPKKTR 370 380 390 400 410 420 EDPLPVPSDQ HSPPFLRLAR STLLLIPLFG IHYTVFAFSP ENVSKRERLV FELGLGSFQG 430 440 450 460 470 480 FVVAVLYCFL NGEVQAEIKR KWRSWKVNRY FTMDFKHRHP SLASSGVNGG TQLSILSKSS 490 SQLRMSSLPA DNLAT « Hide
Isoform PACAP-R [UniParc]. Checksum: 9F6180156064CFC6 Show »	FASTA	467	53,233
10 20 30 40 50 60 MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS PGCPGMWDNI 70 80 90 100 110 120 TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA DSNSLEITDM GVVGRNCTED 130 140 150 160 170 180 GWSEPFPHYF DACGFDDYEP ESGDQDYYYL SVKALYTVGY STSLATLTTA MVILCRFRKL 190 200 210 220 230 240 HCTRNFIHMN LFVSFMLRAI SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY 250 260 270 280 290 300 FWLFIEGLYL FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 310 320 330 340 350 360 STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLRL ARSTLLLIPL 370 380 390 400 410 420 FGIHYTVFAF SPENVSKRER LVFELGLGSF QGFVVAVLYC FLNGEVQAEI KRKWRSWKVN 430 440 450 460 RYFTMDFKHR HPSLASSGVN GGTQLSILSK SSSQLRMSSL PADNLAT « Hide

References

[1]	"Molecular cloning and functional expression of the pituitary adenylate cyclase-activating polypeptide type I receptor." Wank S.A., Pisegna J.R. Proc. Natl. Acad. Sci. U.S.A. 90:6345-6349(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[2]	"Molecular cloning and functional expression of rat cDNAs encoding the receptor for pituitary adenylate cyclase activating polypeptide (PACAP)." Hosoya M., Onda H., Ogi K., Masuda Y., Miyamoto Y., Ohtaki T., Okazaki H., Arimura A., Fujino M. Biochem. Biophys. Res. Commun. 194:133-143(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide." Hashimoto H., Ishihara T., Shigemoto R., Mori K., Nagata S. Neuron 11:333-342(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[4]	"Differential signal transduction by five splice variants of the PACAP receptor." Spengler D., Waeber C., Pantaloni C., Holsboer F., Bockaert J., Seeburg P.H., Journot L. Nature 365:170-175(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[5]	"Molecular cloning and expression of a cDNA encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP)." Morrow J.A., Lutz E.M., West K.M., Fink G., Harmar A.J. FEBS Lett. 329:99-105(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Olfactory bulb.
[6]	Svoboda M., Ciccarelli E., Tastenoy M., Christophe J. Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-523. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16465 mRNA. Translation: BAA03932.1. L16680 mRNA. Translation: AAA41792.1. D14908 mRNA. Translation: BAA03608.1. D14909 mRNA. Translation: BAA03609.1. Z23272 mRNA. Translation: CAA80810.1. Z23273 mRNA. Translation: CAA80811.1. Z23274 mRNA. Translation: CAA80812.1. Z23275 mRNA. Translation: CAA80813.1. Z23279 mRNA. Translation: CAA80817.1. Z23282 mRNA. Translation: CAA80821.1. Z23282 mRNA. Translation: CAA80820.1. L16506 mRNA. Translation: AAA02990.1. Z22735 mRNA. Translation: CAA80429.1.
IPI	IPI00214249. IPI00231305. IPI00231306. IPI00231307. IPI00231308.
PIR	JN0616. S33449. S36114. S39060. S39061.
RefSeq	NP_001257508.1. NM_001270579.1. NP_001257509.1. NM_001270580.1. NP_001257510.1. NM_001270581.1. NP_001257511.1. NM_001270582.1. NP_001257512.1. NM_001270583.1. NP_598195.1. NM_133511.2.
UniGene	Rn.7552. Rn.88408.
3D structure databases
ProteinModelPortal	P32215.
SMR	P32215. Positions 21-142.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000016175.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P32215.
Proteomic databases
PRIDE	P32215.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098.
GeneID	24167.
KEGG	rno:24167.
UCSC	RGD:2038. rat.
Organism-specific databases
CTD	117.
RGD	2038. Adcyap1r1.
Phylogenomic databases
eggNOG	NOG330024.
GeneTree	ENSGT00620000087538.
HOGENOM	HOG000008249.
HOVERGEN	HBG008318.
InParanoid	P32215.
KO	K04587.
OMA	KWRSWKV.
OrthoDB	EOG4B5P5B.
Enzyme and pathway databases
Reactome	REACT_111984. Signal Transduction.
Gene expression databases
Genevestigator	P32215.
GermOnline	ENSRNOG00000012098. Rattus norvegicus.
Family and domain databases
InterPro	IPR017981. GPCR_2-like. IPR001879. GPCR_2_extracellular_dom. IPR002285. GPCR_2_PACAP_1_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
PANTHER	PTHR12011:SF30. PTHR12011:SF30. 1 hit.
Pfam	PF00002. 7tm_2. 1 hit. PF02793. HRM. 1 hit. [Graphical view]
PRINTS	PR00249. GPCRSECRETIN. PR01156. PACAPRECEPTR.
SMART	SM00008. HormR. 1 hit. [Graphical view]
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075233.
NextBio	602477.

Entry information

Entry name

PACR_RAT

Accession

Primary (citable) accession number: P32215
Secondary accession number(s): Q63414

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents