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P32215

- PACR_RAT

UniProt

P32215 - PACR_RAT

Protein

Pituitary adenylate cyclase-activating polypeptide type I receptor

Gene

Adcyap1r1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

    GO - Molecular functioni

    1. adenylate cyclase binding Source: RGD
    2. ADP-ribosylation factor binding Source: RGD
    3. neuropeptide binding Source: RGD
    4. vasoactive intestinal polypeptide receptor activity Source: InterPro

    GO - Biological processi

    1. activation of phospholipase C activity Source: RGD
    2. cAMP-mediated signaling Source: RGD
    3. cell differentiation Source: UniProtKB-KW
    4. development of primary female sexual characteristics Source: RGD
    5. multicellular organismal response to stress Source: RGD
    6. negative regulation of cell death Source: RGD
    7. positive regulation of calcium ion transport into cytosol Source: RGD
    8. positive regulation of cAMP biosynthetic process Source: RGD
    9. positive regulation of inositol phosphate biosynthetic process Source: RGD
    10. response to drug Source: RGD
    11. response to estradiol Source: RGD
    12. response to ethanol Source: RGD
    13. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pituitary adenylate cyclase-activating polypeptide type I receptor
    Short name:
    PACAP type I receptor
    Short name:
    PACAP-R-1
    Short name:
    PACAP-R1
    Gene namesi
    Name:Adcyap1r1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2038. Adcyap1r1.

    Subcellular locationi

    GO - Cellular componenti

    1. caveola Source: RGD
    2. cell surface Source: RGD
    3. cytoplasm Source: RGD
    4. endosome Source: RGD
    5. Golgi membrane Source: Reactome
    6. integral component of membrane Source: UniProtKB-KW
    7. neuron projection Source: RGD
    8. rough endoplasmic reticulum Source: RGD
    9. tight junction Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 523504Pituitary adenylate cyclase-activating polypeptide type I receptorPRO_0000012843Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 62By similarity
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi53 ↔ 117By similarity
    Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi76 ↔ 133By similarity
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP32215.

    PTM databases

    PhosphoSiteiP32215.

    Expressioni

    Tissue specificityi

    Hypothalamus, anterior pituitary, adrenal medulla, testicular germ cells.

    Gene expression databases

    GenevestigatoriP32215.

    Interactioni

    Subunit structurei

    Interacts (via N-terminal extracellular domain) with ADCYAP1.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016175.

    Structurei

    3D structure databases

    ProteinModelPortaliP32215.
    SMRiP32215. Positions 21-142.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 154135ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini178 – 1858CytoplasmicSequence Analysis
    Topological domaini205 – 22622ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini253 – 26715CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini291 – 30818ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini332 – 40574CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini427 – 44014ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini461 – 52363CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei155 – 17723Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei186 – 20419Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei227 – 25226Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei268 – 29023Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei309 – 33123Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei406 – 42621Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei441 – 46020Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 13815Important for ligand binding and specificityBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG330024.
    GeneTreeiENSGT00620000087538.
    HOGENOMiHOG000008249.
    HOVERGENiHBG008318.
    InParanoidiP32215.
    KOiK04587.
    OMAiKWRSWKV.
    OrthoDBiEOG7TF78W.
    PhylomeDBiP32215.
    TreeFamiTF315710.

    Family and domain databases

    InterProiIPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR002285. GPCR_2_PACAP_1_rcpt.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view]
    PfamiPF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view]
    PRINTSiPR00249. GPCRSECRETIN.
    PR01156. PACAPRECEPTR.
    SMARTiSM00008. HormR. 1 hit.
    [Graphical view]
    PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform HIP-HOP1 (identifier: P32215-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS    50
    PGCPGMWDNI TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA 100
    DSNSLEITDM GVVGRNCTED GWSEPFPHYF DACGFDDYEP ESGDQDYYYL 150
    SVKALYTVGY STSLATLTTA MVILCRFRKL HCTRNFIHMN LFVSFMLRAI 200
    SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY FWLFIEGLYL 250
    FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND 300
    STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLTN 350
    LRLRVPKKTR EDPLPVPSDQ HSPPFLSCVQ KCYCKPQRAQ QHSCKMSELS 400
    TITLRLARST LLLIPLFGIH YTVFAFSPEN VSKRERLVFE LGLGSFQGFV 450
    VAVLYCFLNG EVQAEIKRKW RSWKVNRYFT MDFKHRHPSL ASSGVNGGTQ 500
    LSILSKSSSQ LRMSSLPADN LAT 523
    Length:523
    Mass (Da):59,637
    Last modified:February 1, 1994 - v2
    Checksum:i038CA413EED37E44
    GO
    Isoform HOP1 (identifier: P32215-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         348-348: L → F
         349-376: Missing.

    Show »
    Length:495
    Mass (Da):56,450
    Checksum:iCB6A851A3C426176
    GO
    Isoform HOP2 (identifier: P32215-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         348-348: L → F
         349-377: Missing.

    Show »
    Length:494
    Mass (Da):56,363
    Checksum:iC1C969B5075952BD
    GO
    Isoform HIP (identifier: P32215-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         377-404: Missing.

    Show »
    Length:495
    Mass (Da):56,453
    Checksum:i971EBE6AE9901570
    GO
    Isoform PACAP-R (identifier: P32215-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         349-404: Missing.

    Show »
    Length:467
    Mass (Da):53,233
    Checksum:i9F6180156064CFC6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti449 – 4491F → L in AAA41792. (PubMed:8392197)Curated
    Sequence conflicti510 – 5112QL → HV in AAA02990. 1 PublicationCurated
    Sequence conflicti510 – 5112QL → HV in CAA80429. 1 PublicationCurated
    Sequence conflicti515 – 5162SL → TV in AAA02990. 1 PublicationCurated
    Sequence conflicti515 – 5162SL → TV in CAA80429. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei348 – 3481L → F in isoform HOP1 and isoform HOP2. CuratedVSP_002005
    Alternative sequencei349 – 40456Missing in isoform PACAP-R. CuratedVSP_002009Add
    BLAST
    Alternative sequencei349 – 37729Missing in isoform HOP2. CuratedVSP_002007Add
    BLAST
    Alternative sequencei349 – 37628Missing in isoform HOP1. CuratedVSP_002006Add
    BLAST
    Alternative sequencei377 – 40428Missing in isoform HIP. CuratedVSP_002008Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16465 mRNA. Translation: BAA03932.1.
    L16680 mRNA. Translation: AAA41792.1.
    D14908 mRNA. Translation: BAA03608.1.
    D14909 mRNA. Translation: BAA03609.1.
    Z23272 mRNA. Translation: CAA80810.1.
    Z23273 mRNA. Translation: CAA80811.1.
    Z23274 mRNA. Translation: CAA80812.1.
    Z23275 mRNA. Translation: CAA80813.1.
    Z23279 mRNA. Translation: CAA80817.1.
    Z23282 mRNA. Translation: CAA80821.1.
    Z23282 mRNA. Translation: CAA80820.1.
    L16506 mRNA. Translation: AAA02990.1.
    Z22735 mRNA. Translation: CAA80429.1.
    PIRiJN0616.
    S33449.
    S36114.
    S39060.
    S39061.
    RefSeqiNP_001257508.1. NM_001270579.1. [P32215-1]
    NP_001257509.1. NM_001270580.1. [P32215-4]
    NP_001257510.1. NM_001270581.1. [P32215-5]
    NP_001257511.1. NM_001270582.1. [P32215-5]
    NP_001257512.1. NM_001270583.1. [P32215-3]
    NP_598195.1. NM_133511.2. [P32215-2]
    UniGeneiRn.234543.
    Rn.7552.

    Genome annotation databases

    EnsembliENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. [P32215-1]
    ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. [P32215-2]
    ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. [P32215-5]
    ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098. [P32215-4]
    GeneIDi24167.
    KEGGirno:24167.
    UCSCiRGD:2038. rat. [P32215-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16465 mRNA. Translation: BAA03932.1 .
    L16680 mRNA. Translation: AAA41792.1 .
    D14908 mRNA. Translation: BAA03608.1 .
    D14909 mRNA. Translation: BAA03609.1 .
    Z23272 mRNA. Translation: CAA80810.1 .
    Z23273 mRNA. Translation: CAA80811.1 .
    Z23274 mRNA. Translation: CAA80812.1 .
    Z23275 mRNA. Translation: CAA80813.1 .
    Z23279 mRNA. Translation: CAA80817.1 .
    Z23282 mRNA. Translation: CAA80821.1 .
    Z23282 mRNA. Translation: CAA80820.1 .
    L16506 mRNA. Translation: AAA02990.1 .
    Z22735 mRNA. Translation: CAA80429.1 .
    PIRi JN0616.
    S33449.
    S36114.
    S39060.
    S39061.
    RefSeqi NP_001257508.1. NM_001270579.1. [P32215-1 ]
    NP_001257509.1. NM_001270580.1. [P32215-4 ]
    NP_001257510.1. NM_001270581.1. [P32215-5 ]
    NP_001257511.1. NM_001270582.1. [P32215-5 ]
    NP_001257512.1. NM_001270583.1. [P32215-3 ]
    NP_598195.1. NM_133511.2. [P32215-2 ]
    UniGenei Rn.234543.
    Rn.7552.

    3D structure databases

    ProteinModelPortali P32215.
    SMRi P32215. Positions 21-142.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000016175.

    Chemistry

    ChEMBLi CHEMBL1075233.
    GuidetoPHARMACOLOGYi 370.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P32215.

    Proteomic databases

    PRIDEi P32215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016175 ; ENSRNOP00000016175 ; ENSRNOG00000012098 . [P32215-1 ]
    ENSRNOT00000035722 ; ENSRNOP00000030759 ; ENSRNOG00000012098 . [P32215-2 ]
    ENSRNOT00000043851 ; ENSRNOP00000044267 ; ENSRNOG00000012098 . [P32215-5 ]
    ENSRNOT00000046192 ; ENSRNOP00000050048 ; ENSRNOG00000012098 . [P32215-4 ]
    GeneIDi 24167.
    KEGGi rno:24167.
    UCSCi RGD:2038. rat. [P32215-1 ]

    Organism-specific databases

    CTDi 117.
    RGDi 2038. Adcyap1r1.

    Phylogenomic databases

    eggNOGi NOG330024.
    GeneTreei ENSGT00620000087538.
    HOGENOMi HOG000008249.
    HOVERGENi HBG008318.
    InParanoidi P32215.
    KOi K04587.
    OMAi KWRSWKV.
    OrthoDBi EOG7TF78W.
    PhylomeDBi P32215.
    TreeFami TF315710.

    Miscellaneous databases

    NextBioi 602477.
    PROi P32215.

    Gene expression databases

    Genevestigatori P32215.

    Family and domain databases

    InterProi IPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR002285. GPCR_2_PACAP_1_rcpt.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view ]
    Pfami PF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view ]
    PRINTSi PR00249. GPCRSECRETIN.
    PR01156. PACAPRECEPTR.
    SMARTi SM00008. HormR. 1 hit.
    [Graphical view ]
    PROSITEi PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of the pituitary adenylate cyclase-activating polypeptide type I receptor."
      Wank S.A., Pisegna J.R.
      Proc. Natl. Acad. Sci. U.S.A. 90:6345-6349(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.
    2. "Molecular cloning and functional expression of rat cDNAs encoding the receptor for pituitary adenylate cyclase activating polypeptide (PACAP)."
      Hosoya M., Onda H., Ogi K., Masuda Y., Miyamoto Y., Ohtaki T., Okazaki H., Arimura A., Fujino M.
      Biochem. Biophys. Res. Commun. 194:133-143(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide."
      Hashimoto H., Ishihara T., Shigemoto R., Mori K., Nagata S.
      Neuron 11:333-342(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pituitary.
    4. "Differential signal transduction by five splice variants of the PACAP receptor."
      Spengler D., Waeber C., Pantaloni C., Holsboer F., Bockaert J., Seeburg P.H., Journot L.
      Nature 365:170-175(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
    5. "Molecular cloning and expression of a cDNA encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP)."
      Morrow J.A., Lutz E.M., West K.M., Fink G., Harmar A.J.
      FEBS Lett. 329:99-105(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Olfactory bulb.
    6. Svoboda M., Ciccarelli E., Tastenoy M., Christophe J.
      Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-523.
      Strain: Wistar.

    Entry informationi

    Entry nameiPACR_RAT
    AccessioniPrimary (citable) accession number: P32215
    Secondary accession number(s): Q63414
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3