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Protein

Pituitary adenylate cyclase-activating polypeptide type I receptor

Gene

Adcyap1r1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

GO - Molecular functioni

  • adenylate cyclase binding Source: RGD
  • ADP-ribosylation factor binding Source: RGD
  • neuropeptide binding Source: RGD
  • pituitary adenylate cyclase-activating polypeptide receptor activity Source: Reactome
  • vasoactive intestinal polypeptide receptor activity Source: InterPro

GO - Biological processi

  • activation of adenylate cyclase activity Source: Reactome
  • activation of phospholipase C activity Source: RGD
  • cAMP-mediated signaling Source: RGD
  • cell differentiation Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: InterPro
  • development of primary female sexual characteristics Source: RGD
  • multicellular organismal response to stress Source: RGD
  • negative regulation of cell death Source: RGD
  • positive regulation of calcium ion transport into cytosol Source: RGD
  • positive regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of inositol phosphate biosynthetic process Source: RGD
  • positive regulation of small GTPase mediated signal transduction Source: ParkinsonsUK-UCL
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to ethanol Source: RGD
  • spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Differentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-RNO-187024. NGF-independant TRKA activation.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-420092. Glucagon-type ligand receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary adenylate cyclase-activating polypeptide type I receptor
Short name:
PACAP type I receptor
Short name:
PACAP-R-1
Short name:
PACAP-R1
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2038. Adcyap1r1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 154ExtracellularSequence analysisAdd BLAST135
Transmembranei155 – 177Helical; Name=1Sequence analysisAdd BLAST23
Topological domaini178 – 185CytoplasmicSequence analysis8
Transmembranei186 – 204Helical; Name=2Sequence analysisAdd BLAST19
Topological domaini205 – 226ExtracellularSequence analysisAdd BLAST22
Transmembranei227 – 252Helical; Name=3Sequence analysisAdd BLAST26
Topological domaini253 – 267CytoplasmicSequence analysisAdd BLAST15
Transmembranei268 – 290Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini291 – 308ExtracellularSequence analysisAdd BLAST18
Transmembranei309 – 331Helical; Name=5Sequence analysisAdd BLAST23
Topological domaini332 – 405CytoplasmicSequence analysisAdd BLAST74
Transmembranei406 – 426Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini427 – 440ExtracellularSequence analysisAdd BLAST14
Transmembranei441 – 460Helical; Name=7Sequence analysisAdd BLAST20
Topological domaini461 – 523CytoplasmicSequence analysisAdd BLAST63

GO - Cellular componenti

  • bicellular tight junction Source: RGD
  • caveola Source: RGD
  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • endosome Source: RGD
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • neuron projection Source: RGD
  • receptor complex Source: Ensembl
  • rough endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075233.
GuidetoPHARMACOLOGYi370.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001284320 – 523Pituitary adenylate cyclase-activating polypeptide type I receptorAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 62By similarity
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi53 ↔ 117By similarity
Glycosylationi59N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi76 ↔ 133By similarity
Glycosylationi116N-linked (GlcNAc...)Sequence analysis1
Modified residuei489PhosphoserineBy similarity1
Modified residuei502PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP32215.
PRIDEiP32215.

PTM databases

iPTMnetiP32215.
PhosphoSitePlusiP32215.

Expressioni

Tissue specificityi

Hypothalamus, anterior pituitary, adrenal medulla, testicular germ cells.

Gene expression databases

BgeeiENSRNOG00000012098.
GenevisibleiP32215. RN.

Interactioni

Subunit structurei

Interacts (via N-terminal extracellular domain) with ADCYAP1.By similarity

GO - Molecular functioni

  • adenylate cyclase binding Source: RGD
  • ADP-ribosylation factor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016175.

Structurei

3D structure databases

ProteinModelPortaliP32215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 138Important for ligand binding and specificityBy similarityAdd BLAST15

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4564. Eukaryota.
ENOG410XRS2. LUCA.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiP32215.
KOiK04587.
OMAiCKAIMVF.
OrthoDBiEOG091G0NF8.
PhylomeDBiP32215.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002285. GPCR_2_PACAP_1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01156. PACAPRECEPTR.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HIP-HOP1 (identifier: P32215-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARVLQLSLT ALLLPVAIAM HSDCIFKKEQ AMCLERIQRA NDLMGLNESS
60 70 80 90 100
PGCPGMWDNI TCWKPAQVGE MVLVSCPEVF RIFNPDQVWM TETIGDSGFA
110 120 130 140 150
DSNSLEITDM GVVGRNCTED GWSEPFPHYF DACGFDDYEP ESGDQDYYYL
160 170 180 190 200
SVKALYTVGY STSLATLTTA MVILCRFRKL HCTRNFIHMN LFVSFMLRAI
210 220 230 240 250
SVFIKDWILY AEQDSSHCFV STVECKAVMV FFHYCVVSNY FWLFIEGLYL
260 270 280 290 300
FTLLVETFFP ERRYFYWYTI IGWGTPTVCV TVWAVLRLYF DDAGCWDMND
310 320 330 340 350
STALWWVIKG PVVGSIMVNF VLFIGIIIIL VQKLQSPDMG GNESSIYLTN
360 370 380 390 400
LRLRVPKKTR EDPLPVPSDQ HSPPFLSCVQ KCYCKPQRAQ QHSCKMSELS
410 420 430 440 450
TITLRLARST LLLIPLFGIH YTVFAFSPEN VSKRERLVFE LGLGSFQGFV
460 470 480 490 500
VAVLYCFLNG EVQAEIKRKW RSWKVNRYFT MDFKHRHPSL ASSGVNGGTQ
510 520
LSILSKSSSQ LRMSSLPADN LAT
Length:523
Mass (Da):59,637
Last modified:February 1, 1994 - v2
Checksum:i038CA413EED37E44
GO
Isoform HOP1 (identifier: P32215-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: L → F
     349-376: Missing.

Show »
Length:495
Mass (Da):56,450
Checksum:iCB6A851A3C426176
GO
Isoform HOP2 (identifier: P32215-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-348: L → F
     349-377: Missing.

Show »
Length:494
Mass (Da):56,363
Checksum:iC1C969B5075952BD
GO
Isoform HIP (identifier: P32215-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     377-404: Missing.

Show »
Length:495
Mass (Da):56,453
Checksum:i971EBE6AE9901570
GO
Isoform PACAP-R (identifier: P32215-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     349-404: Missing.

Show »
Length:467
Mass (Da):53,233
Checksum:i9F6180156064CFC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti449F → L in AAA41792 (PubMed:8392197).Curated1
Sequence conflicti510 – 511QL → HV in AAA02990 (Ref. 6) Curated2
Sequence conflicti510 – 511QL → HV in CAA80429 (Ref. 6) Curated2
Sequence conflicti515 – 516SL → TV in AAA02990 (Ref. 6) Curated2
Sequence conflicti515 – 516SL → TV in CAA80429 (Ref. 6) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002005348L → F in isoform HOP1 and isoform HOP2. Curated1
Alternative sequenceiVSP_002009349 – 404Missing in isoform PACAP-R. CuratedAdd BLAST56
Alternative sequenceiVSP_002007349 – 377Missing in isoform HOP2. CuratedAdd BLAST29
Alternative sequenceiVSP_002006349 – 376Missing in isoform HOP1. CuratedAdd BLAST28
Alternative sequenceiVSP_002008377 – 404Missing in isoform HIP. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16465 mRNA. Translation: BAA03932.1.
L16680 mRNA. Translation: AAA41792.1.
D14908 mRNA. Translation: BAA03608.1.
D14909 mRNA. Translation: BAA03609.1.
Z23272 mRNA. Translation: CAA80810.1.
Z23273 mRNA. Translation: CAA80811.1.
Z23274 mRNA. Translation: CAA80812.1.
Z23275 mRNA. Translation: CAA80813.1.
Z23279 mRNA. Translation: CAA80817.1.
Z23282 mRNA. Translation: CAA80821.1.
Z23282 mRNA. Translation: CAA80820.1.
L16506 mRNA. Translation: AAA02990.1.
Z22735 mRNA. Translation: CAA80429.1.
PIRiJN0616.
S33449.
S36114.
S39060.
S39061.
RefSeqiNP_001257508.1. NM_001270579.1. [P32215-1]
NP_001257509.1. NM_001270580.1. [P32215-4]
NP_001257510.1. NM_001270581.1. [P32215-5]
NP_001257511.1. NM_001270582.1. [P32215-5]
NP_001257512.1. NM_001270583.1. [P32215-3]
NP_598195.1. NM_133511.2. [P32215-2]
XP_017447918.1. XM_017592429.1. [P32215-2]
XP_017447919.1. XM_017592430.1. [P32215-2]
XP_017447920.1. XM_017592431.1. [P32215-5]
UniGeneiRn.234543.
Rn.7552.

Genome annotation databases

EnsembliENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. [P32215-1]
ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. [P32215-2]
ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. [P32215-5]
ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098. [P32215-4]
GeneIDi24167.
KEGGirno:24167.
UCSCiRGD:2038. rat. [P32215-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16465 mRNA. Translation: BAA03932.1.
L16680 mRNA. Translation: AAA41792.1.
D14908 mRNA. Translation: BAA03608.1.
D14909 mRNA. Translation: BAA03609.1.
Z23272 mRNA. Translation: CAA80810.1.
Z23273 mRNA. Translation: CAA80811.1.
Z23274 mRNA. Translation: CAA80812.1.
Z23275 mRNA. Translation: CAA80813.1.
Z23279 mRNA. Translation: CAA80817.1.
Z23282 mRNA. Translation: CAA80821.1.
Z23282 mRNA. Translation: CAA80820.1.
L16506 mRNA. Translation: AAA02990.1.
Z22735 mRNA. Translation: CAA80429.1.
PIRiJN0616.
S33449.
S36114.
S39060.
S39061.
RefSeqiNP_001257508.1. NM_001270579.1. [P32215-1]
NP_001257509.1. NM_001270580.1. [P32215-4]
NP_001257510.1. NM_001270581.1. [P32215-5]
NP_001257511.1. NM_001270582.1. [P32215-5]
NP_001257512.1. NM_001270583.1. [P32215-3]
NP_598195.1. NM_133511.2. [P32215-2]
XP_017447918.1. XM_017592429.1. [P32215-2]
XP_017447919.1. XM_017592430.1. [P32215-2]
XP_017447920.1. XM_017592431.1. [P32215-5]
UniGeneiRn.234543.
Rn.7552.

3D structure databases

ProteinModelPortaliP32215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016175.

Chemistry databases

ChEMBLiCHEMBL1075233.
GuidetoPHARMACOLOGYi370.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP32215.
PhosphoSitePlusiP32215.

Proteomic databases

PaxDbiP32215.
PRIDEiP32215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016175; ENSRNOP00000016175; ENSRNOG00000012098. [P32215-1]
ENSRNOT00000035722; ENSRNOP00000030759; ENSRNOG00000012098. [P32215-2]
ENSRNOT00000043851; ENSRNOP00000044267; ENSRNOG00000012098. [P32215-5]
ENSRNOT00000046192; ENSRNOP00000050048; ENSRNOG00000012098. [P32215-4]
GeneIDi24167.
KEGGirno:24167.
UCSCiRGD:2038. rat. [P32215-1]

Organism-specific databases

CTDi117.
RGDi2038. Adcyap1r1.

Phylogenomic databases

eggNOGiKOG4564. Eukaryota.
ENOG410XRS2. LUCA.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiP32215.
KOiK04587.
OMAiCKAIMVF.
OrthoDBiEOG091G0NF8.
PhylomeDBiP32215.
TreeFamiTF315710.

Enzyme and pathway databases

ReactomeiR-RNO-187024. NGF-independant TRKA activation.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-420092. Glucagon-type ligand receptors.

Miscellaneous databases

PROiP32215.

Gene expression databases

BgeeiENSRNOG00000012098.
GenevisibleiP32215. RN.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002285. GPCR_2_PACAP_1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01156. PACAPRECEPTR.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPACR_RAT
AccessioniPrimary (citable) accession number: P32215
Secondary accession number(s): Q63414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.