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P32214 (CALCR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcitonin receptor

Short name=CT-R
Alternative name(s):
C1A/C1B
Gene names
Name:Calcr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length516 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.

Subunit structure

Interacts with GPRASP2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 7723741. Source: RGD

negative regulation of ossification

Inferred from electronic annotation. Source: Ensembl

osteoclast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

regulation of mRNA stability

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentacrosomal vesicle

Inferred from electronic annotation. Source: Ensembl

axon

Inferred from direct assay PubMed 15571671. Source: RGD

cilium

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from direct assay PubMed 15571671. Source: RGD

neuronal cell body

Inferred from direct assay PubMed 15571671. Source: RGD

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcitonin binding

Inferred from direct assay PubMed 7723741. Source: RGD

calcitonin receptor activity

Inferred from direct assay PubMed 7723741Ref.2. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: P32214-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: P32214-2)

The sequence of this isoform differs from the canonical sequence as follows:
     217-253: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 516492Calcitonin receptor
PRO_0000012810

Regions

Topological domain25 – 153129Extracellular Potential
Transmembrane154 – 17320Helical; Name=1; Potential
Topological domain174 – 1807Cytoplasmic Potential
Transmembrane181 – 20020Helical; Name=2; Potential
Topological domain201 – 25757Extracellular Potential
Transmembrane258 – 28023Helical; Name=3; Potential
Topological domain281 – 29717Cytoplasmic Potential
Transmembrane298 – 31720Helical; Name=4; Potential
Topological domain318 – 33316Extracellular Potential
Transmembrane334 – 35724Helical; Name=5; Potential
Topological domain358 – 38023Cytoplasmic Potential
Transmembrane381 – 39818Helical; Name=6; Potential
Topological domain399 – 41012Extracellular Potential
Transmembrane411 – 43222Helical; Name=7; Potential
Topological domain433 – 51684Cytoplasmic Potential

Amino acid modifications

Glycosylation281N-linked (GlcNAc...) Potential
Glycosylation731N-linked (GlcNAc...) Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation1301N-linked (GlcNAc...) Potential
Disulfide bond55 ↔ 81 By similarity
Disulfide bond72 ↔ 112 By similarity
Disulfide bond95 ↔ 134 By similarity

Natural variations

Alternative sequence217 – 25337Missing in isoform A.
VSP_001995

Experimental info

Sequence conflict1481L → S in AAA03031. Ref.2
Sequence conflict1481L → S in AAA03030. Ref.2
Sequence conflict4591Missing in AAA03031. Ref.2
Sequence conflict4591Missing in AAA03030. Ref.2
Sequence conflict4791L → R in AAA03031. Ref.2
Sequence conflict4791L → R in AAA03030. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 9B057B860E574378

FASTA51660,292
        10         20         30         40         50         60 
MRFLLLNRFT LLLLLLVSPT PVLQAPTNLT DSGLDQEPFL YLVGRKKLLD AQYKCYDRIQ 

        70         80         90        100        110        120 
QLPPYEGEGP YCNRTWDGWM CWDDTPAGVM SYQHCPDYFP DFDPTEKVSK YCDENGEWFR 

       130        140        150        160        170        180 
HPDSNRTWSN YTLCNAFTPD KLHNAYVLYY LALVGHSMSI AALIASMGIF LFFKNLSCQR 

       190        200        210        220        230        240 
VTLHKNMFLT YILNSIIIII HLVEVVPNGD LVRRDPMHIF HHNTYMWTMQ WELSPPLPLS 

       250        260        270        280        290        300 
AHEGKMDPHD SEVISCKILH FFHQYMMACN YFWMLCEGIY LHTLIVMAVF TEDQRLRWYY 

       310        320        330        340        350        360 
LLGWGFPIVP TIIHAITRAV YYNDNCWLST ETHLLYIIHG PVMAALVVNF FFLLNIVRVL 

       370        380        390        400        410        420 
VTKMRQTHEA EAYMYLKAVK ATMVLVPLLG IQFVVFPWRP SNKVLGKIYD YLMHSLIHFQ 

       430        440        450        460        470        480 
GFFVATIYCF CNHEVQVTLK RQWAQFKIQW SHRWGRRRRP TNRVVSAPRA VAFAEPGGLP 

       490        500        510 
IYICHQEPRN PPVSNNEGEE GTEMIPMNVI QQDSSA 

« Hide

Isoform A [UniParc].

Checksum: 03EE4D1A7F2266A8
Show »

FASTA47955,879

References

[1]"Molecular cloning of two receptors from rat brain with high affinity for salmon calcitonin."
Albrandt K.G., Mull E., Brady E.M., Herich J., Moore C.X., Beaumont K.
FEBS Lett. 325:225-232(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
[2]"Identification of brain isoforms of the rat calcitonin receptor."
Sexton P.M., Housammi S., Hilton J.M., O'Keeffe L.M., Center R.J., Gillespie M.T., Darcy P., Findlay D.M.
Mol. Endocrinol. 7:815-821(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L14618 mRNA. Translation: AAA65965.1.
L14617 mRNA. Translation: AAA65964.1.
L13040 mRNA. Translation: AAA03031.1.
L13041 mRNA. Translation: AAA03030.1.
PIRA37430.
I60800.
S33746.
RefSeqNP_001029187.1. NM_001034015.1.
NP_446268.2. NM_053816.2.
UniGeneRn.10062.

3D structure databases

ProteinModelPortalP32214.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250474. 1 interaction.

Chemistry

BindingDBP32214.
ChEMBLCHEMBL2109231.
GuidetoPHARMACOLOGY43.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32214.

Proteomic databases

PaxDbP32214.
PRIDEP32214.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013910; ENSRNOP00000013910; ENSRNOG00000010053. [P32214-1]
ENSRNOT00000013937; ENSRNOP00000013937; ENSRNOG00000010053. [P32214-2]
GeneID116506.
KEGGrno:116506.
UCSCRGD:621001. rat. [P32214-1]

Organism-specific databases

CTD799.
RGD621001. Calcr.

Phylogenomic databases

eggNOGNOG255868.
GeneTreeENSGT00750000117654.
HOGENOMHOG000230695.
HOVERGENHBG102129.
InParanoidP32214.
KOK04576.
OMATHEAESY.
OrthoDBEOG712TW2.
PhylomeDBP32214.

Gene expression databases

GenevestigatorP32214.

Family and domain databases

InterProIPR003287. GCPR_2_calcitonin_rcpt_fam.
IPR017981. GPCR_2-like.
IPR001688. GPCR_2_calcitonin_rcpt.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR00361. CALCITONINR.
PR01350. CTRFAMILY.
PR00249. GPCRSECRETIN.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619119.
PROP32214.

Entry information

Entry nameCALCR_RAT
AccessionPrimary (citable) accession number: P32214
Secondary accession number(s): P32213
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries