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Protein

Muscarinic acetylcholine receptor M4

Gene

Chrm4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_279397. Muscarinic acetylcholine receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Alternative name(s):
Mm4 mAChR
Gene namesi
Name:Chrm4
Synonyms:Chrm-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88399. Chrm4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030ExtracellularBy similarityAdd
BLAST
Transmembranei31 – 5323Helical; Name=1By similarityAdd
BLAST
Topological domaini54 – 6714CytoplasmicBy similarityAdd
BLAST
Transmembranei68 – 8821Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10517ExtracellularBy similarityAdd
BLAST
Transmembranei106 – 12722Helical; Name=3By similarityAdd
BLAST
Topological domaini128 – 14720CytoplasmicBy similarityAdd
BLAST
Transmembranei148 – 17023Helical; Name=4By similarityAdd
BLAST
Topological domaini171 – 19222ExtracellularBy similarityAdd
BLAST
Transmembranei193 – 21523Helical; Name=5By similarityAdd
BLAST
Topological domaini216 – 401186CytoplasmicBy similarityAdd
BLAST
Transmembranei402 – 42221Helical; Name=6By similarityAdd
BLAST
Topological domaini423 – 43614ExtracellularBy similarityAdd
BLAST
Transmembranei437 – 45620Helical; Name=7By similarityAdd
BLAST
Topological domaini457 – 47923CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Muscarinic acetylcholine receptor M4PRO_0000069038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
Glycosylationi13 – 131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi104 ↔ 184PROSITE-ProRule annotation
Modified residuei459 – 4591PhosphothreonineSequence Analysis
Modified residuei463 – 4631PhosphothreonineSequence Analysis
Modified residuei477 – 4771PhosphothreonineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP32211.
PRIDEiP32211.

PTM databases

PhosphoSiteiP32211.

Expressioni

Gene expression databases

BgeeiP32211.
CleanExiMM_CHRM4.
ExpressionAtlasiP32211. baseline.
GenevisibleiP32211. MM.

Interactioni

Protein-protein interaction databases

IntActiP32211. 1 interaction.
MINTiMINT-7984826.
STRINGi10090.ENSMUSP00000040808.

Structurei

3D structure databases

ProteinModelPortaliP32211.
SMRiP32211. Positions 27-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP32211.
KOiK04132.
OMAiMCQYRNI.
OrthoDBiEOG7V49Z7.
PhylomeDBiP32211.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI
60 70 80 90 100
LVMLSIKVNR QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG
110 120 130 140 150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL
160 170 180 190 200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI
210 220 230 240 250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK
260 270 280 290 300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA
310 320 330 340 350
TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG
360 370 380 390 400
SECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR
410 420 430 440 450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PERVWSIGYW LCYVNSTINP
460 470
ACYALCNATF KKTFRHLLLC QYRNIGTAR
Length:479
Mass (Da):52,973
Last modified:October 1, 1993 - v1
Checksum:i04F301E78814CD70
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti322 – 3232AL → V in AAB33577 (PubMed:7874308).Curated
Sequence conflicti351 – 3511S → N in AAB33577 (PubMed:7874308).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63473 Genomic DNA. Translation: CAA45071.1.
S74916 mRNA. Translation: AAB33577.1.
CCDSiCCDS16439.1.
PIRiS33776.
RefSeqiNP_031725.1. NM_007699.2.
UniGeneiMm.330405.

Genome annotation databases

EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
GeneIDi12672.
KEGGimmu:12672.
UCSCiuc008kwv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63473 Genomic DNA. Translation: CAA45071.1.
S74916 mRNA. Translation: AAB33577.1.
CCDSiCCDS16439.1.
PIRiS33776.
RefSeqiNP_031725.1. NM_007699.2.
UniGeneiMm.330405.

3D structure databases

ProteinModelPortaliP32211.
SMRiP32211. Positions 27-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32211. 1 interaction.
MINTiMINT-7984826.
STRINGi10090.ENSMUSP00000040808.

Chemistry

BindingDBiP32211.
ChEMBLiCHEMBL4572.
GuidetoPHARMACOLOGYi16.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP32211.

Proteomic databases

PaxDbiP32211.
PRIDEiP32211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
GeneIDi12672.
KEGGimmu:12672.
UCSCiuc008kwv.2. mouse.

Organism-specific databases

CTDi1132.
MGIiMGI:88399. Chrm4.

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP32211.
KOiK04132.
OMAiMCQYRNI.
OrthoDBiEOG7V49Z7.
PhylomeDBiP32211.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiREACT_279397. Muscarinic acetylcholine receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi281914.
PROiP32211.
SOURCEiSearch...

Gene expression databases

BgeeiP32211.
CleanExiMM_CHRM4.
ExpressionAtlasiP32211. baseline.
GenevisibleiP32211. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation, sequence and functional expression of the mouse m4 muscarinic acetylcholine receptor gene."
    van Koppen C.J., Lenz W., Nathanson N.M.
    Biochim. Biophys. Acta 1173:342-344(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
    Andre C., Dos Santos G., Koulakoff A.
    Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-355.
    Tissue: Brain.

Entry informationi

Entry nameiACM4_MOUSE
AccessioniPrimary (citable) accession number: P32211
Secondary accession number(s): Q64056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 22, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.