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P32211

- ACM4_MOUSE

UniProt

P32211 - ACM4_MOUSE

Protein

Muscarinic acetylcholine receptor M4

Gene

Chrm4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

    GO - Molecular functioni

    1. G-protein coupled acetylcholine receptor activity Source: MGI

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: MGI
    2. regulation of locomotion Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M4
    Alternative name(s):
    Mm4 mAChR
    Gene namesi
    Name:Chrm4
    Synonyms:Chrm-4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:88399. Chrm4.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of plasma membrane Source: InterPro
    3. membrane Source: MGI
    4. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Muscarinic acetylcholine receptor M4PRO_0000069038Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi13 – 131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi104 ↔ 184PROSITE-ProRule annotation
    Modified residuei459 – 4591PhosphothreonineSequence Analysis
    Modified residuei463 – 4631PhosphothreonineSequence Analysis
    Modified residuei477 – 4771PhosphothreonineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP32211.
    PRIDEiP32211.

    PTM databases

    PhosphoSiteiP32211.

    Expressioni

    Gene expression databases

    BgeeiP32211.
    CleanExiMM_CHRM4.
    GenevestigatoriP32211.

    Interactioni

    Protein-protein interaction databases

    IntActiP32211. 1 interaction.
    MINTiMINT-7984826.

    Structurei

    3D structure databases

    ProteinModelPortaliP32211.
    SMRiP32211. Positions 27-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3030ExtracellularBy similarityAdd
    BLAST
    Topological domaini54 – 6714CytoplasmicBy similarityAdd
    BLAST
    Topological domaini89 – 10517ExtracellularBy similarityAdd
    BLAST
    Topological domaini128 – 14720CytoplasmicBy similarityAdd
    BLAST
    Topological domaini171 – 19222ExtracellularBy similarityAdd
    BLAST
    Topological domaini216 – 401186CytoplasmicBy similarityAdd
    BLAST
    Topological domaini423 – 43614ExtracellularBy similarityAdd
    BLAST
    Topological domaini457 – 47923CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 5323Helical; Name=1By similarityAdd
    BLAST
    Transmembranei68 – 8821Helical; Name=2By similarityAdd
    BLAST
    Transmembranei106 – 12722Helical; Name=3By similarityAdd
    BLAST
    Transmembranei148 – 17023Helical; Name=4By similarityAdd
    BLAST
    Transmembranei193 – 21523Helical; Name=5By similarityAdd
    BLAST
    Transmembranei402 – 42221Helical; Name=6By similarityAdd
    BLAST
    Transmembranei437 – 45620Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiP32211.
    KOiK04132.
    OMAiCIPDTVW.
    OrthoDBiEOG7V49Z7.
    PhylomeDBiP32211.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001432. Musac_Ach_M4_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PANTHERiPTHR24249:SF60. PTHR24249:SF60. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00541. MUSCRINICM4R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32211-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI    50
    LVMLSIKVNR QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG 100
    AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL 150
    MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI 200
    AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK 250
    PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA 300
    TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG 350
    SECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR 400
    TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PERVWSIGYW LCYVNSTINP 450
    ACYALCNATF KKTFRHLLLC QYRNIGTAR 479
    Length:479
    Mass (Da):52,973
    Last modified:October 1, 1993 - v1
    Checksum:i04F301E78814CD70
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti322 – 3232AL → V in AAB33577. (PubMed:7874308)Curated
    Sequence conflicti351 – 3511S → N in AAB33577. (PubMed:7874308)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63473 Genomic DNA. Translation: CAA45071.1.
    S74916 mRNA. Translation: AAB33577.1.
    CCDSiCCDS16439.1.
    PIRiS33776.
    RefSeqiNP_031725.1. NM_007699.2.
    UniGeneiMm.330405.

    Genome annotation databases

    EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
    ENSMUST00000170432; ENSMUSP00000130000; ENSMUSG00000040495.
    GeneIDi12672.
    KEGGimmu:12672.
    UCSCiuc008kwv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63473 Genomic DNA. Translation: CAA45071.1 .
    S74916 mRNA. Translation: AAB33577.1 .
    CCDSi CCDS16439.1.
    PIRi S33776.
    RefSeqi NP_031725.1. NM_007699.2.
    UniGenei Mm.330405.

    3D structure databases

    ProteinModelPortali P32211.
    SMRi P32211. Positions 27-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P32211. 1 interaction.
    MINTi MINT-7984826.

    Chemistry

    BindingDBi P32211.
    ChEMBLi CHEMBL2097162.
    GuidetoPHARMACOLOGYi 16.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P32211.

    Proteomic databases

    PaxDbi P32211.
    PRIDEi P32211.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045537 ; ENSMUSP00000040808 ; ENSMUSG00000040495 .
    ENSMUST00000170432 ; ENSMUSP00000130000 ; ENSMUSG00000040495 .
    GeneIDi 12672.
    KEGGi mmu:12672.
    UCSCi uc008kwv.2. mouse.

    Organism-specific databases

    CTDi 1132.
    MGIi MGI:88399. Chrm4.

    Phylogenomic databases

    eggNOGi NOG250863.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi P32211.
    KOi K04132.
    OMAi CIPDTVW.
    OrthoDBi EOG7V49Z7.
    PhylomeDBi P32211.
    TreeFami TF320495.

    Miscellaneous databases

    NextBioi 281914.
    PROi P32211.
    SOURCEi Search...

    Gene expression databases

    Bgeei P32211.
    CleanExi MM_CHRM4.
    Genevestigatori P32211.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001432. Musac_Ach_M4_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    PANTHERi PTHR24249:SF60. PTHR24249:SF60. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00541. MUSCRINICM4R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequence and functional expression of the mouse m4 muscarinic acetylcholine receptor gene."
      van Koppen C.J., Lenz W., Nathanson N.M.
      Biochim. Biophys. Acta 1173:342-344(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
      Andre C., Dos Santos G., Koulakoff A.
      Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-355.
      Tissue: Brain.

    Entry informationi

    Entry nameiACM4_MOUSE
    AccessioniPrimary (citable) accession number: P32211
    Secondary accession number(s): Q64056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3