Chrm4

Functionⁱ

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functionⁱ

G-protein coupled acetylcholine receptor activity
Source: MGI
Inferred from direct assayⁱ
- 7916637

Complete GO annotation...

GO - Biological processⁱ

adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 7916637
phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
regulation of locomotion Source: InterPro
synaptic transmission, cholinergic Source: GO_Central

Complete GO annotation...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Receptor, Transducer

Molecular function

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

Reactomeⁱ	R-MMU-390648. Muscarinic acetylcholine receptors. R-MMU-418594. G alpha (i) signalling events.

Reactomeⁱ

R-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Muscarinic acetylcholine receptor M4 Alternative name(s): Mm4 mAChR
Gene namesⁱ	Name:Chrm4 Synonyms:Chrm-4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:88399. Chrm4.

Subcellular locationⁱ

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 30	ExtracellularBy similarityAdd BLAST	30
Transmembraneⁱ	31 – 53	Helical; Name=1By similarityAdd BLAST	23
Topological domainⁱ	54 – 67	CytoplasmicBy similarityAdd BLAST	14
Transmembraneⁱ	68 – 88	Helical; Name=2By similarityAdd BLAST	21
Topological domainⁱ	89 – 105	ExtracellularBy similarityAdd BLAST	17
Transmembraneⁱ	106 – 127	Helical; Name=3By similarityAdd BLAST	22
Topological domainⁱ	128 – 147	CytoplasmicBy similarityAdd BLAST	20
Transmembraneⁱ	148 – 170	Helical; Name=4By similarityAdd BLAST	23
Topological domainⁱ	171 – 192	ExtracellularBy similarityAdd BLAST	22
Transmembraneⁱ	193 – 215	Helical; Name=5By similarityAdd BLAST	23
Topological domainⁱ	216 – 401	CytoplasmicBy similarityAdd BLAST	186
Transmembraneⁱ	402 – 422	Helical; Name=6By similarityAdd BLAST	21
Topological domainⁱ	423 – 436	ExtracellularBy similarityAdd BLAST	14
Transmembraneⁱ	437 – 456	Helical; Name=7By similarityAdd BLAST	20
Topological domainⁱ	457 – 479	CytoplasmicBy similarityAdd BLAST	23

GO - Cellular componentⁱ

cell junction Source: UniProtKB-KW
integral component of plasma membrane Source: InterPro
membrane
Source: MGI
Inferred from direct assayⁱ
- 7916637
postsynaptic membrane Source: UniProtKB-SubCell
synapse Source: GO_Central

Complete GO annotation...

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL4572.

ChEMBLⁱ

CHEMBL4572.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000069038	1 – 479	Muscarinic acetylcholine receptor M4Add BLAST		479

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	8	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	13	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	104 ↔ 184	PROSITE-ProRule annotation
Modified residueⁱ	459	PhosphothreonineSequence analysis	1
Modified residueⁱ	463	PhosphothreonineSequence analysis	1
Modified residueⁱ	477	PhosphothreonineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbⁱ	P32211.
PRIDEⁱ	P32211.

PTM databases

iPTMnetⁱ	P32211.
PhosphoSitePlusⁱ	P32211.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000040495.
CleanExⁱ	MM_CHRM4.
ExpressionAtlasⁱ	P32211. baseline and differential.
Genevisibleⁱ	P32211. MM.

Interactionⁱ

Protein-protein interaction databases

IntActⁱ	P32211. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-7984826.
STRINGⁱ	10090.ENSMUSP00000040808.

Chemistry databases

BindingDBⁱ

P32211.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P32211.
SMRⁱ	P32211.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4220. Eukaryota. ENOG410YCQR. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00780000121874.
HOGENOMⁱ	HOG000231484.
HOVERGENⁱ	HBG105720.
InParanoidⁱ	P32211.
KEGG Orthology (KO) More...KOⁱ	K04132.
OMAⁱ	TSTHNRY.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06VI.
PhylomeDBⁱ	P32211.
TreeFamⁱ	TF320495.

Family and domain databases

InterProⁱ	View protein in InterPro IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001432. Musac_Ach_M4_rcpt. IPR000995. Musac_Ach_rcpt.
PANTHERⁱ	PTHR24249:SF349. PTHR24249:SF349. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001. 7tm_1. 1 hit.
PRINTSⁱ	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00541. MUSCRINICM4R.
SMARTⁱ	View protein in SMART SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P32211-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI 
        60         70         80         90        100
LVMLSIKVNR QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG 
       110        120        130        140        150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL 
       160        170        180        190        200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI 
       210        220        230        240        250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK 
       260        270        280        290        300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA 
       310        320        330        340        350
TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG 
       360        370        380        390        400
SECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR 
       410        420        430        440        450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PERVWSIGYW LCYVNSTINP 
       460        470 
ACYALCNATF KKTFRHLLLC QYRNIGTAR

Length:479

Mass (Da):52,973

Last modified:October 1, 1993 - v1

Checksum:ⁱ04F301E78814CD70

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	322 – 323	AL → V in AAB33577 (PubMed:7874308).Curated		2
Sequence conflictⁱ	351	S → N in AAB33577 (PubMed:7874308).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X63473 Genomic DNA. Translation: CAA45071.1. S74916 mRNA. Translation: AAB33577.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16439.1.
PIRⁱ	S33776.
NCBI Reference Sequences More...RefSeqⁱ	NP_031725.1. NM_007699.2.
UniGeneⁱ	Mm.330405.

Genome annotation databases

Ensemblⁱ	ENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
GeneIDⁱ	12672.
KEGGⁱ	mmu:12672.
UCSC genome browser More...UCSCⁱ	uc008kwv.2. mouse.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X63473 Genomic DNA. Translation: CAA45071.1. S74916 mRNA. Translation: AAB33577.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16439.1.
PIRⁱ	S33776.
NCBI Reference Sequences More...RefSeqⁱ	NP_031725.1. NM_007699.2.
UniGeneⁱ	Mm.330405.

3D structure databases

ProteinModelPortalⁱ	P32211.
SMRⁱ	P32211.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P32211. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-7984826.
STRINGⁱ	10090.ENSMUSP00000040808.

Chemistry databases

BindingDBⁱ	P32211.
ChEMBLⁱ	CHEMBL4572.

Protein family/group databases

GPCRDBⁱ	Search...

GPCRDBⁱ

Search...

PTM databases

iPTMnetⁱ	P32211.
PhosphoSitePlusⁱ	P32211.

Proteomic databases

PaxDbⁱ	P32211.
PRIDEⁱ	P32211.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
GeneIDⁱ	12672.
KEGGⁱ	mmu:12672.
UCSC genome browser More...UCSCⁱ	uc008kwv.2. mouse.

Organism-specific databases

CTDⁱ	1132.
MGIⁱ	MGI:88399. Chrm4.

Phylogenomic databases

eggNOGⁱ	KOG4220. Eukaryota. ENOG410YCQR. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00780000121874.
HOGENOMⁱ	HOG000231484.
HOVERGENⁱ	HBG105720.
InParanoidⁱ	P32211.
KEGG Orthology (KO) More...KOⁱ	K04132.
OMAⁱ	TSTHNRY.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06VI.
PhylomeDBⁱ	P32211.
TreeFamⁱ	TF320495.

Enzyme and pathway databases

Reactomeⁱ	R-MMU-390648. Muscarinic acetylcholine receptors. R-MMU-418594. G alpha (i) signalling events.

Reactomeⁱ

R-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P32211.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000040495.
CleanExⁱ	MM_CHRM4.
ExpressionAtlasⁱ	P32211. baseline and differential.
Genevisibleⁱ	P32211. MM.

Family and domain databases

InterProⁱ	View protein in InterPro IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001432. Musac_Ach_M4_rcpt. IPR000995. Musac_Ach_rcpt.
PANTHERⁱ	PTHR24249:SF349. PTHR24249:SF349. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001. 7tm_1. 1 hit.
PRINTSⁱ	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00541. MUSCRINICM4R.
SMARTⁱ	View protein in SMART SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACM4_MOUSE
Accessionⁱ	P32211Primary (citable) accession number: P32211 Secondary accession number(s): Q64056
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
	Last sequence update:	October 1, 1993
	Last modified:	June 7, 2017
	This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P32211 (ACM4_MOUSE)

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Muscarinic acetylcholine receptor M4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ