Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muscarinic acetylcholine receptor M4

Gene

Chrm4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

  • G-protein coupled acetylcholine receptor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Alternative name(s):
Mm4 mAChR
Gene namesi
Name:Chrm4
Synonyms:Chrm-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88399. Chrm4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30ExtracellularBy similarityAdd BLAST30
Transmembranei31 – 53Helical; Name=1By similarityAdd BLAST23
Topological domaini54 – 67CytoplasmicBy similarityAdd BLAST14
Transmembranei68 – 88Helical; Name=2By similarityAdd BLAST21
Topological domaini89 – 105ExtracellularBy similarityAdd BLAST17
Transmembranei106 – 127Helical; Name=3By similarityAdd BLAST22
Topological domaini128 – 147CytoplasmicBy similarityAdd BLAST20
Transmembranei148 – 170Helical; Name=4By similarityAdd BLAST23
Topological domaini171 – 192ExtracellularBy similarityAdd BLAST22
Transmembranei193 – 215Helical; Name=5By similarityAdd BLAST23
Topological domaini216 – 401CytoplasmicBy similarityAdd BLAST186
Transmembranei402 – 422Helical; Name=6By similarityAdd BLAST21
Topological domaini423 – 436ExtracellularBy similarityAdd BLAST14
Transmembranei437 – 456Helical; Name=7By similarityAdd BLAST20
Topological domaini457 – 479CytoplasmicBy similarityAdd BLAST23

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of plasma membrane Source: InterPro
  • membrane Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4572.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690381 – 479Muscarinic acetylcholine receptor M4Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...)Sequence analysis1
Glycosylationi13N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi104 ↔ 184PROSITE-ProRule annotation
Modified residuei459PhosphothreonineSequence analysis1
Modified residuei463PhosphothreonineSequence analysis1
Modified residuei477PhosphothreonineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP32211.
PRIDEiP32211.

PTM databases

iPTMnetiP32211.
PhosphoSitePlusiP32211.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040495.
CleanExiMM_CHRM4.
ExpressionAtlasiP32211. baseline and differential.
GenevisibleiP32211. MM.

Interactioni

Protein-protein interaction databases

IntActiP32211. 1 interactor.
MINTiMINT-7984826.
STRINGi10090.ENSMUSP00000040808.

Chemistry databases

BindingDBiP32211.

Structurei

3D structure databases

ProteinModelPortaliP32211.
SMRiP32211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP32211.
KOiK04132.
OMAiMCQYRNI.
OrthoDBiEOG091G06VI.
PhylomeDBiP32211.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI
60 70 80 90 100
LVMLSIKVNR QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG
110 120 130 140 150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL
160 170 180 190 200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI
210 220 230 240 250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK
260 270 280 290 300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA
310 320 330 340 350
TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG
360 370 380 390 400
SECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR
410 420 430 440 450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PERVWSIGYW LCYVNSTINP
460 470
ACYALCNATF KKTFRHLLLC QYRNIGTAR
Length:479
Mass (Da):52,973
Last modified:October 1, 1993 - v1
Checksum:i04F301E78814CD70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti322 – 323AL → V in AAB33577 (PubMed:7874308).Curated2
Sequence conflicti351S → N in AAB33577 (PubMed:7874308).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63473 Genomic DNA. Translation: CAA45071.1.
S74916 mRNA. Translation: AAB33577.1.
CCDSiCCDS16439.1.
PIRiS33776.
RefSeqiNP_031725.1. NM_007699.2.
UniGeneiMm.330405.

Genome annotation databases

EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
GeneIDi12672.
KEGGimmu:12672.
UCSCiuc008kwv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63473 Genomic DNA. Translation: CAA45071.1.
S74916 mRNA. Translation: AAB33577.1.
CCDSiCCDS16439.1.
PIRiS33776.
RefSeqiNP_031725.1. NM_007699.2.
UniGeneiMm.330405.

3D structure databases

ProteinModelPortaliP32211.
SMRiP32211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32211. 1 interactor.
MINTiMINT-7984826.
STRINGi10090.ENSMUSP00000040808.

Chemistry databases

BindingDBiP32211.
ChEMBLiCHEMBL4572.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP32211.
PhosphoSitePlusiP32211.

Proteomic databases

PaxDbiP32211.
PRIDEiP32211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
GeneIDi12672.
KEGGimmu:12672.
UCSCiuc008kwv.2. mouse.

Organism-specific databases

CTDi1132.
MGIiMGI:88399. Chrm4.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP32211.
KOiK04132.
OMAiMCQYRNI.
OrthoDBiEOG091G06VI.
PhylomeDBiP32211.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP32211.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040495.
CleanExiMM_CHRM4.
ExpressionAtlasiP32211. baseline and differential.
GenevisibleiP32211. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM4_MOUSE
AccessioniPrimary (citable) accession number: P32211
Secondary accession number(s): Q64056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.