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P32211 (ACM4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M4
Alternative name(s):
Mm4 mAChR
Gene names
Name:Chrm4
Synonyms:Chrm-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Muscarinic acetylcholine receptor M4
PRO_0000069038

Regions

Topological domain1 – 3030Extracellular By similarity
Transmembrane31 – 5323Helical; Name=1; By similarity
Topological domain54 – 6714Cytoplasmic By similarity
Transmembrane68 – 8821Helical; Name=2; By similarity
Topological domain89 – 10517Extracellular By similarity
Transmembrane106 – 12722Helical; Name=3; By similarity
Topological domain128 – 14720Cytoplasmic By similarity
Transmembrane148 – 17023Helical; Name=4; By similarity
Topological domain171 – 19222Extracellular By similarity
Transmembrane193 – 21523Helical; Name=5; By similarity
Topological domain216 – 401186Cytoplasmic By similarity
Transmembrane402 – 42221Helical; Name=6; By similarity
Topological domain423 – 43614Extracellular By similarity
Transmembrane437 – 45620Helical; Name=7; By similarity
Topological domain457 – 47923Cytoplasmic By similarity

Amino acid modifications

Modified residue4591Phosphothreonine Potential
Modified residue4631Phosphothreonine Potential
Modified residue4771Phosphothreonine Potential
Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation131N-linked (GlcNAc...) Potential
Disulfide bond104 ↔ 184 By similarity

Experimental info

Sequence conflict322 – 3232AL → V in AAB33577. Ref.2
Sequence conflict3511S → N in AAB33577. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P32211 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 04F301E78814CD70

FASTA47952,973
        10         20         30         40         50         60 
MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI LVMLSIKVNR 

        70         80         90        100        110        120 
QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG AVVCDLWLAL DYVVSNASVM 

       130        140        150        160        170        180 
NLLIISFDRY FCVTKPLTYP ARRTTKMAGL MIAAAWVLSF VLWAPAILFW QFVVGKRTVP 

       190        200        210        220        230        240 
DNQCFIQFLS NPAVTFGTAI AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT 

       250        260        270        280        290        300 
LAFLKSPLMK PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA 

       310        320        330        340        350        360 
TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG SECVTAIEIV 

       370        380        390        400        410        420 
PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR TIFAILLAFI LTWTPYNVMV 

       430        440        450        460        470 
LVNTFCQSCI PERVWSIGYW LCYVNSTINP ACYALCNATF KKTFRHLLLC QYRNIGTAR 

« Hide

References

[1]"Isolation, sequence and functional expression of the mouse m4 muscarinic acetylcholine receptor gene."
van Koppen C.J., Lenz W., Nathanson N.M.
Biochim. Biophys. Acta 1173:342-344(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
Andre C., Dos Santos G., Koulakoff A.
Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-355.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63473 Genomic DNA. Translation: CAA45071.1.
S74916 mRNA. Translation: AAB33577.1.
CCDSCCDS16439.1.
PIRS33776.
RefSeqNP_031725.1. NM_007699.2.
UniGeneMm.330405.

3D structure databases

ProteinModelPortalP32211.
SMRP32211. Positions 27-469.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP32211. 1 interaction.
MINTMINT-7984826.

Chemistry

BindingDBP32211.
ChEMBLCHEMBL2097162.
GuidetoPHARMACOLOGY16.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP32211.

Proteomic databases

PaxDbP32211.
PRIDEP32211.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
ENSMUST00000170432; ENSMUSP00000130000; ENSMUSG00000040495.
GeneID12672.
KEGGmmu:12672.
UCSCuc008kwv.2. mouse.

Organism-specific databases

CTD1132.
MGIMGI:88399. Chrm4.

Phylogenomic databases

eggNOGNOG250863.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP32211.
KOK04132.
OMACIPDTVW.
OrthoDBEOG7V49Z7.
PhylomeDBP32211.
TreeFamTF320495.

Gene expression databases

BgeeP32211.
CleanExMM_CHRM4.
GenevestigatorP32211.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF60. PTHR24249:SF60. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281914.
PROP32211.
SOURCESearch...

Entry information

Entry nameACM4_MOUSE
AccessionPrimary (citable) accession number: P32211
Secondary accession number(s): Q64056
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries