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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

C14L

Organism
Swinepox virus (strain Kasza) (SWPV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 2By similarity
Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
Active sitei108 – 1081PROSITE-ProRule annotation
Metal bindingi163 – 1631Iron 2By similarity
Metal bindingi197 – 1971Iron 2By similarity
Metal bindingi200 – 2001Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
ORF Names:C14L
OrganismiSwinepox virus (strain Kasza) (SWPV)
Taxonomic identifieri10277 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeSuipoxvirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Ribonucleoside-diphosphate reductase small chainPRO_0000190500Add
BLAST

Proteomic databases

PRIDEiP32209.

Interactioni

Subunit structurei

Heterodimer of a large and a small chain.

Structurei

3D structure databases

ProteinModelPortaliP32209.
SMRiP32209. Positions 2-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPILQESDS RFVIFPIKYH DIWKMYKQSV ASFWTVEEVD LSKDLDDWDK
60 70 80 90 100
LTKDEKYFIK HILAFFASSD GIVNENLAER FYVDVQCSEA RCFYGFQIAM
110 120 130 140 150
ENIHSEMYSL LIDTYVRDNI EKMHLFNAIE TMECVKKKAD WARKWISSNK
160 170 180 190 200
VYGERVVAFA AVEGIFFSGS FAAIFWIKKR GLMPGLTFSN ELISRDEGLH
210 220 230 240 250
CDFACLMFKH LLHPPSKEVI TSIIIDAVNI EKEFLTVAIP VDLIGMNCCL
260 270
MSQYIEFVAD RLLTELGCEK SQCI
Length:274
Mass (Da):31,753
Last modified:October 1, 1993 - v1
Checksum:iF6F1A309EC75F411
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22013 Unassigned RNA. Translation: AAC37857.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22013 Unassigned RNA. Translation: AAC37857.1.

3D structure databases

ProteinModelPortaliP32209.
SMRiP32209. Positions 2-270.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP32209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DNA sequence analysis of conserved and unique regions of swinepox virus: identification of genetic elements supporting phenotypic observations including a novel G protein-coupled receptor homologue."
    Massung R.F., Jayarama V., Moyer R.W.
    Virology 197:511-528(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiRIR2_SWPVK
AccessioniPrimary (citable) accession number: P32209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.