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Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

GUT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol-3-phosphate dehydrogenase, mitochondrial (GUT2)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 9729FADSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • glycerol-3-phosphate metabolic process Source: InterPro
  • glycerol catabolic process Source: UniProtKB-UniPathway
  • glycerol metabolic process Source: SGD
  • NADH oxidation Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:YIL155C-MONOMER.
ReactomeiR-SCE-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
SABIO-RKP32191.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
Short name:
GPD-M
Short name:
GPDH-M
Gene namesi
Name:GUT2
Ordered Locus Names:YIL155C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL155C.
SGDiS000001417. GUT2.

Subcellular locationi

GO - Cellular componenti

  • glycerol-3-phosphate dehydrogenase complex Source: InterPro
  • integral component of mitochondrial outer membrane Source: SGD
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 649Glycerol-3-phosphate dehydrogenase, mitochondrialPRO_0000010434
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP32191.
PRIDEiP32191.

PTM databases

iPTMnetiP32191.

Interactioni

Protein-protein interaction databases

BioGridi34837. 34 interactions.
IntActiP32191. 1 interaction.
MINTiMINT-4490257.

Structurei

3D structure databases

ProteinModelPortaliP32191.
SMRiP32191. Positions 64-638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000001718.
HOGENOMiHOG000004813.
InParanoidiP32191.
KOiK00111.
OMAiAMRNELT.
OrthoDBiEOG7NSBB6.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR031656. DAO_C.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16901. DAO_C. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSVTRRRAA GAAAAMATAT GTLYWMTSQG DRPLVHNDPS YMVQFPTAAP
60 70 80 90 100
PQVSRRDLLD RLAKTHQFDV LIIGGGATGT GCALDAATRG LNVALVEKGD
110 120 130 140 150
FASGTSSKST KMIHGGVRYL EKAFWEFSKA QLDLVIEALN ERKHLINTAP
160 170 180 190 200
HLCTVLPILI PIYSTWQVPY IYMGCKFYDF FAGSQNLKKS YLLSKSATVE
210 220 230 240 250
KAPMLTTDNL KASLVYHDGS FNDSRLNATL AITAVENGAT VLNYVEVQKL
260 270 280 290 300
IKDPTSGKVI GAEARDVETN ELVRINAKCV VNATGPYSDA ILQMDRNPSG
310 320 330 340 350
LPDSPLNDNS KIKSTFNQIA VMDPKMVIPS IGVHIVLPSF YCPKDMGLLD
360 370 380 390 400
VRTSDGRVMF FLPWQGKVLA GTTDIPLKQV PENPMPTEAD IQDILKELQH
410 420 430 440 450
YIEFPVKRED VLSAWAGVRP LVRDPRTIPA DGKKGSATQG VVRSHFLFTS
460 470 480 490 500
DNGLITIAGG KWTTYRQMAE ETVDKVVEVG GFHNLKPCHT RDIKLAGAEE
510 520 530 540 550
WTQNYVALLA QNYHLSSKMS NYLVQNYGTR SSIICEFFKE SMENKLPLSL
560 570 580 590 600
ADKENNVIYS SEENNLVNFD TFRYPFTIGE LKYSMQYEYC RTPLDFLLRR
610 620 630 640
TRFAFLDAKE ALNAVHATVK VMGDEFNWSE KKRQWELEKT VNFIKTFGV
Length:649
Mass (Da):72,389
Last modified:February 1, 1995 - v2
Checksum:iFE6B25F5B21EF8DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 5151MFSVT…TAAPP → MTRATWCNSPPPLHR (PubMed:8256521).CuratedAdd
BLAST
Sequence conflicti63 – 631A → D in CAA50652 (PubMed:8256521).Curated
Sequence conflicti182 – 1821A → G in CAA50652 (PubMed:8256521).Curated
Sequence conflicti234 – 2341A → G in CAA50652 (PubMed:8256521).Curated
Sequence conflicti243 – 2431N → I in CAA50652 (PubMed:8256521).Curated
Sequence conflicti249 – 2491K → R in AAT92886 (PubMed:17322287).Curated
Sequence conflicti320 – 3201A → S in CAA50652 (PubMed:8256521).Curated
Sequence conflicti342 – 3421C → S in CAA50652 (PubMed:8256521).Curated
Sequence conflicti645 – 6462KT → QGR in CAA50652 (PubMed:8256521).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA. Translation: CAA86123.1.
X71660 Genomic DNA. Translation: CAA50652.1.
AY692867 Genomic DNA. Translation: AAT92886.1.
BK006942 Genomic DNA. Translation: DAA08398.1.
PIRiS38190. S48379.
RefSeqiNP_012111.1. NM_001179503.1.

Genome annotation databases

EnsemblFungiiYIL155C; YIL155C; YIL155C.
GeneIDi854651.
KEGGisce:YIL155C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA. Translation: CAA86123.1.
X71660 Genomic DNA. Translation: CAA50652.1.
AY692867 Genomic DNA. Translation: AAT92886.1.
BK006942 Genomic DNA. Translation: DAA08398.1.
PIRiS38190. S48379.
RefSeqiNP_012111.1. NM_001179503.1.

3D structure databases

ProteinModelPortaliP32191.
SMRiP32191. Positions 64-638.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34837. 34 interactions.
IntActiP32191. 1 interaction.
MINTiMINT-4490257.

PTM databases

iPTMnetiP32191.

Proteomic databases

MaxQBiP32191.
PRIDEiP32191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL155C; YIL155C; YIL155C.
GeneIDi854651.
KEGGisce:YIL155C.

Organism-specific databases

EuPathDBiFungiDB:YIL155C.
SGDiS000001417. GUT2.

Phylogenomic databases

GeneTreeiENSGT00390000001718.
HOGENOMiHOG000004813.
InParanoidiP32191.
KOiK00111.
OMAiAMRNELT.
OrthoDBiEOG7NSBB6.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00673.
BioCyciYEAST:YIL155C-MONOMER.
ReactomeiR-SCE-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
SABIO-RKP32191.

Miscellaneous databases

PROiP32191.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR031656. DAO_C.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16901. DAO_C. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae."
    Roennow B., Kielland-Brandt M.C.
    Yeast 9:1121-1130(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGPDM_YEAST
AccessioniPrimary (citable) accession number: P32191
Secondary accession number(s): D6VVD2, E9P8Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.