P32191 (GPDM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial
Short name=GPD-M
Short name=GPDH-M
EC=1.1.5.3

Gene names

Name:	GUT2
Ordered Locus Names:	YIL155C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.
Subcellular location	Mitochondrion inner membrane Ref.5.
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from direct assay. Source: SGD glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro replicative cell aging Inferred from mutant phenotype. Source: SGD
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro integral to mitochondrial outer membrane Inferred from direct assay. Source: SGD mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 649		Glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000010434
Regions
	Nucleotide binding	69 – 97	29	FAD Potential
Experimental info
	Sequence conflict	1 – 51	51	MFSVT…TAAPP → MTRATWCNSPPPLHR Ref.1
	Sequence conflict	63	1	A → D in CAA50652. Ref.1
	Sequence conflict	182	1	A → G in CAA50652. Ref.1
	Sequence conflict	234	1	A → G in CAA50652. Ref.1
	Sequence conflict	243	1	N → I in CAA50652. Ref.1
	Sequence conflict	249	1	K → R in AAT92886. Ref.4
	Sequence conflict	320	1	A → S in CAA50652. Ref.1
	Sequence conflict	342	1	C → S in CAA50652. Ref.1
	Sequence conflict	645 – 646	2	KT → QGR in CAA50652. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32191 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: FE6B25F5B21EF8DA	FASTA	649	72,389
	10 20 30 40 50 60 MFSVTRRRAA GAAAAMATAT GTLYWMTSQG DRPLVHNDPS YMVQFPTAAP PQVSRRDLLD 70 80 90 100 110 120 RLAKTHQFDV LIIGGGATGT GCALDAATRG LNVALVEKGD FASGTSSKST KMIHGGVRYL 130 140 150 160 170 180 EKAFWEFSKA QLDLVIEALN ERKHLINTAP HLCTVLPILI PIYSTWQVPY IYMGCKFYDF 190 200 210 220 230 240 FAGSQNLKKS YLLSKSATVE KAPMLTTDNL KASLVYHDGS FNDSRLNATL AITAVENGAT 250 260 270 280 290 300 VLNYVEVQKL IKDPTSGKVI GAEARDVETN ELVRINAKCV VNATGPYSDA ILQMDRNPSG 310 320 330 340 350 360 LPDSPLNDNS KIKSTFNQIA VMDPKMVIPS IGVHIVLPSF YCPKDMGLLD VRTSDGRVMF 370 380 390 400 410 420 FLPWQGKVLA GTTDIPLKQV PENPMPTEAD IQDILKELQH YIEFPVKRED VLSAWAGVRP 430 440 450 460 470 480 LVRDPRTIPA DGKKGSATQG VVRSHFLFTS DNGLITIAGG KWTTYRQMAE ETVDKVVEVG 490 500 510 520 530 540 GFHNLKPCHT RDIKLAGAEE WTQNYVALLA QNYHLSSKMS NYLVQNYGTR SSIICEFFKE 550 560 570 580 590 600 SMENKLPLSL ADKENNVIYS SEENNLVNFD TFRYPFTIGE LKYSMQYEYC RTPLDFLLRR 610 620 630 640 TRFAFLDAKE ALNAVHATVK VMGDEFNWSE KKRQWELEKT VNFIKTFGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae." Roennow B., Kielland-Brandt M.C. Yeast 9:1121-1130(1993) [PubMed: 8256521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38059 Genomic DNA. Translation: CAA86123.1. X71660 Genomic DNA. Translation: CAA50652.1. AY692867 Genomic DNA. Translation: AAT92886.1. BK006942 Genomic DNA. Translation: DAA08398.1.
PIR	S48379. S38190.
RefSeq	NP_012111.1. NM_001179503.1.
3D structure databases
ProteinModelPortal	P32191.
SMR	P32191. Positions 52-645.
ModBase	Search...
Protein-protein interaction databases
STRING	P32191.
Proteomic databases
PeptideAtlas	P32191.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL155C; YIL155C; YIL155C.
GeneID	854651.
KEGG	sce:YIL155C.
NMPDR	fig|4932.3.peg.1633.
Organism-specific databases
CYGD	YIL155c.
SGD	S000001417. GUT2.
Phylogenomic databases
eggNOG	fuNOG04353.
HOGENOM	HBG720894.
OMA	DSIRKMD.
OrthoDB	EOG46MFT1.
Gene expression databases
ArrayExpress	P32191.
Genevestigator	P32191.
GermOnline	YIL155C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR000447. G3P_DH_FAD-dep. [Graphical view]
KO	K00111.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977202.

Entry information

Entry name

GPDM_YEAST

Accession

Primary (citable) accession number: P32191
Secondary accession number(s): D6VVD2, E9P8Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program