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P32191 (GPDM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial

Short name=GPD-M
Short name=GPDH-M
EC=1.1.5.3
Gene names
Name:GUT2
Ordered Locus Names:YIL155C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length649 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactor

FAD.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.

Subcellular location

Mitochondrion inner membrane Ref.5.

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 649Glycerol-3-phosphate dehydrogenase, mitochondrialPRO_0000010434

Regions

Nucleotide binding69 – 9729FAD Potential

Experimental info

Sequence conflict1 – 5151MFSVT…TAAPP → MTRATWCNSPPPLHR Ref.1
Sequence conflict631A → D in CAA50652. Ref.1
Sequence conflict1821A → G in CAA50652. Ref.1
Sequence conflict2341A → G in CAA50652. Ref.1
Sequence conflict2431N → I in CAA50652. Ref.1
Sequence conflict2491K → R in AAT92886. Ref.4
Sequence conflict3201A → S in CAA50652. Ref.1
Sequence conflict3421C → S in CAA50652. Ref.1
Sequence conflict645 – 6462KT → QGR in CAA50652. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32191 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: FE6B25F5B21EF8DA

FASTA64972,389
        10         20         30         40         50         60 
MFSVTRRRAA GAAAAMATAT GTLYWMTSQG DRPLVHNDPS YMVQFPTAAP PQVSRRDLLD 

        70         80         90        100        110        120 
RLAKTHQFDV LIIGGGATGT GCALDAATRG LNVALVEKGD FASGTSSKST KMIHGGVRYL 

       130        140        150        160        170        180 
EKAFWEFSKA QLDLVIEALN ERKHLINTAP HLCTVLPILI PIYSTWQVPY IYMGCKFYDF 

       190        200        210        220        230        240 
FAGSQNLKKS YLLSKSATVE KAPMLTTDNL KASLVYHDGS FNDSRLNATL AITAVENGAT 

       250        260        270        280        290        300 
VLNYVEVQKL IKDPTSGKVI GAEARDVETN ELVRINAKCV VNATGPYSDA ILQMDRNPSG 

       310        320        330        340        350        360 
LPDSPLNDNS KIKSTFNQIA VMDPKMVIPS IGVHIVLPSF YCPKDMGLLD VRTSDGRVMF 

       370        380        390        400        410        420 
FLPWQGKVLA GTTDIPLKQV PENPMPTEAD IQDILKELQH YIEFPVKRED VLSAWAGVRP 

       430        440        450        460        470        480 
LVRDPRTIPA DGKKGSATQG VVRSHFLFTS DNGLITIAGG KWTTYRQMAE ETVDKVVEVG 

       490        500        510        520        530        540 
GFHNLKPCHT RDIKLAGAEE WTQNYVALLA QNYHLSSKMS NYLVQNYGTR SSIICEFFKE 

       550        560        570        580        590        600 
SMENKLPLSL ADKENNVIYS SEENNLVNFD TFRYPFTIGE LKYSMQYEYC RTPLDFLLRR 

       610        620        630        640 
TRFAFLDAKE ALNAVHATVK VMGDEFNWSE KKRQWELEKT VNFIKTFGV 

« Hide

References

« Hide 'large scale' references
[1]"GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae."
Roennow B., Kielland-Brandt M.C.
Yeast 9:1121-1130(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38059 Genomic DNA. Translation: CAA86123.1.
X71660 Genomic DNA. Translation: CAA50652.1.
AY692867 Genomic DNA. Translation: AAT92886.1.
BK006942 Genomic DNA. Translation: DAA08398.1.
PIRS48379. S38190.
RefSeqNP_012111.1. NM_001179503.1.

3D structure databases

ProteinModelPortalP32191.
SMRP32191. Positions 67-638.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34837. 34 interactions.
MINTMINT-4490257.
STRING4932.YIL155C.

Proteomic databases

PaxDbP32191.
PeptideAtlasP32191.
PRIDEP32191.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL155C; YIL155C; YIL155C.
GeneID854651.
KEGGsce:YIL155C.

Organism-specific databases

CYGDYIL155c.
SGDS000001417. GUT2.

Phylogenomic databases

eggNOGCOG0578.
GeneTreeENSGT00390000001718.
HOGENOMHOG000004813.
KOK00111.
OMAANRFGSN.
OrthoDBEOG7NSBB6.

Enzyme and pathway databases

BioCycYEAST:YIL155C-MONOMER.
SABIO-RKP32191.
UniPathwayUPA00618; UER00673.

Gene expression databases

GenevestigatorP32191.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
PROSITEPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977202.
PROP32191.

Entry information

Entry nameGPDM_YEAST
AccessionPrimary (citable) accession number: P32191
Secondary accession number(s): D6VVD2, E9P8Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways