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Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

GUT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol-3-phosphate dehydrogenase, mitochondrial (GUT2)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 97FADSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

  • glycerol-3-phosphate metabolic process Source: InterPro
  • glycerol catabolic process Source: UniProtKB-UniPathway
  • glycerol metabolic process Source: SGD
  • NADH oxidation Source: SGD
  • replicative cell aging Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:YIL155C-MONOMER
ReactomeiR-SCE-1483166 Synthesis of PA
R-SCE-163560 Triglyceride catabolism
SABIO-RKP32191
UniPathwayiUPA00618; UER00673

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
Short name:
GPD-M
Short name:
GPDH-M
Gene namesi
Name:GUT2
Ordered Locus Names:YIL155C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL155C
SGDiS000001417 GUT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000010434? – 649Glycerol-3-phosphate dehydrogenase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP32191
PaxDbiP32191
PRIDEiP32191

PTM databases

iPTMnetiP32191

Interactioni

Protein-protein interaction databases

BioGridi34837, 73 interactors
DIPiDIP-7385N
IntActiP32191, 3 interactors
STRINGi4932.YIL155C

Structurei

3D structure databases

ProteinModelPortaliP32191
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000001718
HOGENOMiHOG000004813
InParanoidiP32191
KOiK00111
OMAiVPYYWVG
OrthoDBiEOG092C1H88

Family and domain databases

Gene3Di1.10.8.870, 1 hit
3.50.50.60, 4 hits
InterProiView protein in InterPro
IPR031656 DAO_C
IPR038299 DAO_C_sf
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR000447 G3P_DH_FAD-dep
PANTHERiPTHR11985 PTHR11985, 1 hit
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PF16901 DAO_C, 1 hit
PRINTSiPR01001 FADG3PDH
SUPFAMiSSF51905 SSF51905, 2 hits
PROSITEiView protein in PROSITE
PS00977 FAD_G3PDH_1, 1 hit
PS00978 FAD_G3PDH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSVTRRRAA GAAAAMATAT GTLYWMTSQG DRPLVHNDPS YMVQFPTAAP
60 70 80 90 100
PQVSRRDLLD RLAKTHQFDV LIIGGGATGT GCALDAATRG LNVALVEKGD
110 120 130 140 150
FASGTSSKST KMIHGGVRYL EKAFWEFSKA QLDLVIEALN ERKHLINTAP
160 170 180 190 200
HLCTVLPILI PIYSTWQVPY IYMGCKFYDF FAGSQNLKKS YLLSKSATVE
210 220 230 240 250
KAPMLTTDNL KASLVYHDGS FNDSRLNATL AITAVENGAT VLNYVEVQKL
260 270 280 290 300
IKDPTSGKVI GAEARDVETN ELVRINAKCV VNATGPYSDA ILQMDRNPSG
310 320 330 340 350
LPDSPLNDNS KIKSTFNQIA VMDPKMVIPS IGVHIVLPSF YCPKDMGLLD
360 370 380 390 400
VRTSDGRVMF FLPWQGKVLA GTTDIPLKQV PENPMPTEAD IQDILKELQH
410 420 430 440 450
YIEFPVKRED VLSAWAGVRP LVRDPRTIPA DGKKGSATQG VVRSHFLFTS
460 470 480 490 500
DNGLITIAGG KWTTYRQMAE ETVDKVVEVG GFHNLKPCHT RDIKLAGAEE
510 520 530 540 550
WTQNYVALLA QNYHLSSKMS NYLVQNYGTR SSIICEFFKE SMENKLPLSL
560 570 580 590 600
ADKENNVIYS SEENNLVNFD TFRYPFTIGE LKYSMQYEYC RTPLDFLLRR
610 620 630 640
TRFAFLDAKE ALNAVHATVK VMGDEFNWSE KKRQWELEKT VNFIKTFGV
Length:649
Mass (Da):72,389
Last modified:February 1, 1995 - v2
Checksum:iFE6B25F5B21EF8DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 51MFSVT…TAAPP → MTRATWCNSPPPLHR (PubMed:8256521).CuratedAdd BLAST51
Sequence conflicti63A → D in CAA50652 (PubMed:8256521).Curated1
Sequence conflicti182A → G in CAA50652 (PubMed:8256521).Curated1
Sequence conflicti234A → G in CAA50652 (PubMed:8256521).Curated1
Sequence conflicti243N → I in CAA50652 (PubMed:8256521).Curated1
Sequence conflicti249K → R in AAT92886 (PubMed:17322287).Curated1
Sequence conflicti320A → S in CAA50652 (PubMed:8256521).Curated1
Sequence conflicti342C → S in CAA50652 (PubMed:8256521).Curated1
Sequence conflicti645 – 646KT → QGR in CAA50652 (PubMed:8256521).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA Translation: CAA86123.1
X71660 Genomic DNA Translation: CAA50652.1
AY692867 Genomic DNA Translation: AAT92886.1
BK006942 Genomic DNA Translation: DAA08398.1
PIRiS38190 S48379
RefSeqiNP_012111.1, NM_001179503.1

Genome annotation databases

EnsemblFungiiYIL155C; YIL155C; YIL155C
GeneIDi854651
KEGGisce:YIL155C

Similar proteinsi

Entry informationi

Entry nameiGPDM_YEAST
AccessioniPrimary (citable) accession number: P32191
Secondary accession number(s): D6VVD2, E9P8Y2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health