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Protein

Glycerol kinase

Gene

GUT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateBy similarity
Binding sitei60 – 601ATPBy similarity
Binding sitei201 – 2011SubstrateBy similarity
Binding sitei258 – 2581SubstrateBy similarity
Binding sitei386 – 3861SubstrateBy similarity
Binding sitei408 – 4081ATPBy similarity
Binding sitei463 – 4631ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi584 – 5885ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glycerol kinase activity Source: SGD

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: InterPro
  2. glycerol catabolic process Source: UniProtKB-UniPathway
  3. glycerol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YHL032C-MONOMER.
ReactomeiREACT_234352. Triglyceride Biosynthesis.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinase (EC:2.7.1.30)
Short name:
GK
Short name:
Glycerokinase
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Gene namesi
Name:GUT1
Ordered Locus Names:YHL032C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHL032c.
SGDiS000001024. GUT1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Glycerol kinasePRO_0000059542Add
BLAST

Proteomic databases

MaxQBiP32190.
PaxDbiP32190.

Expressioni

Gene expression databases

GenevestigatoriP32190.

Interactioni

Protein-protein interaction databases

BioGridi36390. 28 interactions.
DIPiDIP-6761N.
IntActiP32190. 3 interactions.
MINTiMINT-641670.
STRINGi4932.YHL032C.

Structurei

3D structure databases

ProteinModelPortaliP32190.
SMRiP32190. Positions 147-696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FGGY kinase family.Curated

Phylogenomic databases

eggNOGiCOG0554.
GeneTreeiENSGT00530000063143.
HOGENOMiHOG000222134.
InParanoidiP32190.
KOiK00864.
OMAiKYSMGAS.
OrthoDBiEOG7DRJBQ.

Family and domain databases

InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32190-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPSLFRLVV FSKRYIFRSS QRLYTSLKQE QSRMSKIMED LRSDYVPLIA
60 70 80 90 100
SIDVGTTSSR CILFNRWGQD VSKHQIEYST SASKGKIGVS GLRRPSTAPA
110 120 130 140 150
RETPNAGDIK TSGKPIFSAE GYAIQETKFL KIEELDLDFH NEPTLKFPKP
160 170 180 190 200
GWVECHPQKL LVNVVQCLAS SLLSLQTINS ERVANGLPPY KVICMGIANM
210 220 230 240 250
RETTILWSRR TGKPIVNYGI VWNDTRTIKI VRDKWQNTSV DRQLQLRQKT
260 270 280 290 300
GLPLLSTYFS CSKLRWFLDN EPLCTKAYEE NDLMFGTVDT WLIYQLTKQK
310 320 330 340 350
AFVSDVTNAS RTGFMNLSTL KYDNELLEFW GIDKNLIHMP EIVSSSQYYG
360 370 380 390 400
DFGIPDWIME KLHDSPKTVL RDLVKRNLPI QGCLGDQSAS MVGQLAYKPG
410 420 430 440 450
AAKCTYGTGC FLLYNTGTKK LISQHGALTT LAFWFPHLQE YGGQKPELSK
460 470 480 490 500
PHFALEGSVA VAGAVVQWLR DNLRLIDKSE DVGPIASTVP DSGGVVFVPA
510 520 530 540 550
FSGLFAPYWD PDARATIMGM SQFTTASHIA RAAVEGVCFQ ARAILKAMSS
560 570 580 590 600
DAFGEGSKDR DFLEEISDVT YEKSPLSVLA VDGGMSRSNE VMQIQADILG
610 620 630 640 650
PCVKVRRSPT AECTALGAAI AANMAFKDVN ERPLWKDLHD VKKWVFYNGM
660 670 680 690 700
EKNEQISPEA HPNLKIFRSE SDDAERRKHW KYWEVAVERS KGWLKDIEGE

HEQVLENFQ
Length:709
Mass (Da):79,824
Last modified:October 1, 1993 - v1
Checksum:i12B42C4DABE49FF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69049 Genomic DNA. Translation: CAA48791.1.
U11583 Genomic DNA. Translation: AAB65044.1.
BK006934 Genomic DNA. Translation: DAA06653.1.
PIRiS33907.
RefSeqiNP_011831.1. NM_001179112.1.

Genome annotation databases

EnsemblFungiiYHL032C; YHL032C; YHL032C.
GeneIDi856353.
KEGGisce:YHL032C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69049 Genomic DNA. Translation: CAA48791.1.
U11583 Genomic DNA. Translation: AAB65044.1.
BK006934 Genomic DNA. Translation: DAA06653.1.
PIRiS33907.
RefSeqiNP_011831.1. NM_001179112.1.

3D structure databases

ProteinModelPortaliP32190.
SMRiP32190. Positions 147-696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36390. 28 interactions.
DIPiDIP-6761N.
IntActiP32190. 3 interactions.
MINTiMINT-641670.
STRINGi4932.YHL032C.

Proteomic databases

MaxQBiP32190.
PaxDbiP32190.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL032C; YHL032C; YHL032C.
GeneIDi856353.
KEGGisce:YHL032C.

Organism-specific databases

CYGDiYHL032c.
SGDiS000001024. GUT1.

Phylogenomic databases

eggNOGiCOG0554.
GeneTreeiENSGT00530000063143.
HOGENOMiHOG000222134.
InParanoidiP32190.
KOiK00864.
OMAiKYSMGAS.
OrthoDBiEOG7DRJBQ.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciYEAST:YHL032C-MONOMER.
ReactomeiREACT_234352. Triglyceride Biosynthesis.

Miscellaneous databases

NextBioi981796.
PROiP32190.

Gene expression databases

GenevestigatoriP32190.

Family and domain databases

InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization."
    Pavlik P., Simon M., Schuster T., Ruis H.
    Curr. Genet. 24:21-25(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiGLPK_YEAST
AccessioniPrimary (citable) accession number: P32190
Secondary accession number(s): D3DKT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 7, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.