P32190 (GLPK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
Short name=GK
Short name=Glycerokinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase

Gene names

Name:	GUT1
Ordered Locus Names:	YHL032C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Glycerol kinase		PRO_0000059542
Regions
	Nucleotide binding	584 – 588	5	ATP By similarity
Sites
	Binding site	56	1	Substrate By similarity
	Binding site	60	1	ATP By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	386	1	Substrate By similarity
	Binding site	408	1	ATP By similarity
	Binding site	463	1	ATP; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	96	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P32190 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 12B42C4DABE49FF6	FASTA	709	79,824
	10 20 30 40 50 60 MFPSLFRLVV FSKRYIFRSS QRLYTSLKQE QSRMSKIMED LRSDYVPLIA SIDVGTTSSR 70 80 90 100 110 120 CILFNRWGQD VSKHQIEYST SASKGKIGVS GLRRPSTAPA RETPNAGDIK TSGKPIFSAE 130 140 150 160 170 180 GYAIQETKFL KIEELDLDFH NEPTLKFPKP GWVECHPQKL LVNVVQCLAS SLLSLQTINS 190 200 210 220 230 240 ERVANGLPPY KVICMGIANM RETTILWSRR TGKPIVNYGI VWNDTRTIKI VRDKWQNTSV 250 260 270 280 290 300 DRQLQLRQKT GLPLLSTYFS CSKLRWFLDN EPLCTKAYEE NDLMFGTVDT WLIYQLTKQK 310 320 330 340 350 360 AFVSDVTNAS RTGFMNLSTL KYDNELLEFW GIDKNLIHMP EIVSSSQYYG DFGIPDWIME 370 380 390 400 410 420 KLHDSPKTVL RDLVKRNLPI QGCLGDQSAS MVGQLAYKPG AAKCTYGTGC FLLYNTGTKK 430 440 450 460 470 480 LISQHGALTT LAFWFPHLQE YGGQKPELSK PHFALEGSVA VAGAVVQWLR DNLRLIDKSE 490 500 510 520 530 540 DVGPIASTVP DSGGVVFVPA FSGLFAPYWD PDARATIMGM SQFTTASHIA RAAVEGVCFQ 550 560 570 580 590 600 ARAILKAMSS DAFGEGSKDR DFLEEISDVT YEKSPLSVLA VDGGMSRSNE VMQIQADILG 610 620 630 640 650 660 PCVKVRRSPT AECTALGAAI AANMAFKDVN ERPLWKDLHD VKKWVFYNGM EKNEQISPEA 670 680 690 700 HPNLKIFRSE SDDAERRKHW KYWEVAVERS KGWLKDIEGE HEQVLENFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization." Pavlik P., Simon M., Schuster T., Ruis H. Curr. Genet. 24:21-25(1993) [PubMed: 8358828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69049 Genomic DNA. Translation: CAA48791.1. U11583 Genomic DNA. Translation: AAB65044.1. BK006934 Genomic DNA. Translation: DAA06653.1.
PIR	S33907.
RefSeq	NP_011831.1. NM_001179112.1.
3D structure databases
ProteinModelPortal	P32190.
SMR	P32190. Positions 48-696.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6761N.
IntAct	P32190. 3 interactions.
MINT	MINT-641670.
STRING	P32190.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL032C; YHL032C; YHL032C.
GeneID	856353.
KEGG	sce:YHL032C.
NMPDR	fig|4932.3.peg.2968.
Organism-specific databases
CYGD	YHL032c.
SGD	S000001024. GUT1.
Phylogenomic databases
eggNOG	fuNOG06418.
GeneTree	EFGT00050000004175.
HOGENOM	HBG511469.
OMA	QFTTASH.
OrthoDB	EOG4BZR9S.
Gene expression databases
ArrayExpress	P32190.
Genevestigator	P32190.
GermOnline	YHL032C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981796.

Entry information

Entry name

GLPK_YEAST

Accession

Primary (citable) accession number: P32190
Secondary accession number(s): D3DKT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program