Reviewed, UniProtKB/Swiss-Prot P32185 (3HIDH_RABIT)

Names and origin

Protein names

Recommended name:
    3-hydroxyisobutyrate dehydrogenase
      Short name=HIBADH
    EC=1.1.1.31

Gene names

Name:

HIBADH

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	35 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›35	›35	3-hydroxyisobutyrate dehydrogenase		PRO_0000173054
Regions
	Nucleotide binding	4 – 33	30	NAD By similarity
Experimental info
	Non-terminal residue	35	1

Sequences

	Sequence		Length	Mass (Da)
	P32185-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: C15D62589F84ED64	FASTA	35	3,796
	10 20 30 ASKTPVGFIG LGNMGNPMAK NLMKHGYPLI IYDVF

References

[1]

"Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase. Evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases." Rougraff P.M., Zhang B., Kuntz M.J., Harris R.A., Crabb D.W. J. Biol. Chem. 264:5899-5903(1989) [PubMed: 2647728] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P32185.
Enzyme and pathway databases
BRENDA	1.1.1.31. 255.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR006115. 6PGDH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3HIDH_RABIT

Accession

Primary (citable) accession number: P32185

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)