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P32179 (MET22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3'(2'),5'-bisphosphate nucleotidase

EC=3.1.3.7
Alternative name(s):
3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
DPNPase
Halotolerance protein HAL2
Methionine-requiring protein 22
Gene names
Name:MET22
Synonyms:HAL2
Ordered Locus Names:YOL064C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance. Confers resistance to lithium.

Catalytic activity

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.

Cofactor

Magnesium.

Subcellular location

Cytoplasm. Nucleus Ref.6.

Induction

By salt stress.

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the inositol monophosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3573573'(2'),5'-bisphosphate nucleotidase
PRO_0000142537

Regions

Region144 – 1474Substrate binding By similarity

Sites

Metal binding721Magnesium 1
Metal binding1421Magnesium 1
Metal binding1421Magnesium 2
Metal binding1441Magnesium 1; via carbonyl oxygen
Metal binding1451Magnesium 2
Metal binding2941Magnesium 2
Binding site721Substrate By similarity
Binding site2941Substrate By similarity

Natural variations

Natural variant401N → S in strain: Montrache.
Natural variant611K → M in strain: Montrache.
Natural variant631N → S in strain: Montrache.
Natural variant3081I → V in strain: Montrache.

Secondary structure

.................................................................. 357
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32179 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: AB2E5F90B285702B

FASTA35739,149
        10         20         30         40         50         60 
MALERELLVA TQAVRKASLL TKRIQSEVIS HKDSTTITKN DNSPVTTGDY AAQTIIINAI 

        70         80         90        100        110        120 
KSNFPDDKVV GEESSSGLSD AFVSGILNEI KANDEVYNKN YKKDDFLFTN DQFPLKSLED 

       130        140        150        160        170        180 
VRQIIDFGNY EGGRKGRFWC LDPIDGTKGF LRGEQFAVCL ALIVDGVVQL GCIGCPNLVL 

       190        200        210        220        230        240 
SSYGAQDLKG HESFGYIFRA VRGLGAFYSP SSDAESWTKI HVRHLKDTKD MITLEGVEKG 

       250        260        270        280        290        300 
HSSHDEQTAI KNKLNISKSL HLDSQAKYCL LALGLADVYL RLPIKLSYQE KIWDHAAGNV 

       310        320        330        340        350 
IVHEAGGIHT DAMEDVPLDF GNGRTLATKG VIASSGPREL HDLVVSTSCD VIQSRNA 

« Hide

References

« Hide 'large scale' references
[1]"Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene."
Glaeser H.-U., Thomas D., Gaxiola R., Montrichard F., Surdin-Kerjan Y., Serrano R.
EMBO J. 12:3105-3110(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning of HAL2 gene from Saccharomyces cerevisiae Montrache."
Thanananta N., Apisitwanich S., Peyachoknagul S.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Montrache.
[3]"Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
Tzermia M., Katsoulou C., Alexandraki D.
Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"X-ray structure of yeast Hal2p, a major target of lithium and sodium toxicity, and identification of framework interactions determining cation sensitivity."
Albert A., Yenush L., Gil-Mascarell M.R., Rodriguez P.L., Patel S., Martinez-Ripoll M., Blundell T.L., Serrano R.
J. Mol. Biol. 295:927-938(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS AND AMP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72847 Genomic DNA. Translation: CAA51361.1.
AY500154 Genomic DNA. Translation: AAR89916.1.
Z74806 Genomic DNA. Translation: CAA99074.1.
BK006948 Genomic DNA. Translation: DAA10719.1.
PIRS35318.
RefSeqNP_014577.1. NM_001183319.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K9YX-ray1.90A1-357[»]
1K9ZX-ray1.50A1-357[»]
1KA0X-ray1.80A1-357[»]
1KA1X-ray1.30A1-357[»]
1QGXX-ray1.60A1-357[»]
ProteinModelPortalP32179.
SMRP32179. Positions 2-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34337. 139 interactions.
DIPDIP-4072N.
IntActP32179. 1 interaction.
MINTMINT-506588.
STRING4932.YOL064C.

Chemistry

DrugBankDB00131. Adenosine monophosphate.

Proteomic databases

MaxQBP32179.
PaxDbP32179.
PeptideAtlasP32179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL064C; YOL064C; YOL064C.
GeneID854090.
KEGGsce:YOL064C.

Organism-specific databases

CYGDYOL064c.
SGDS000005425. MET22.

Phylogenomic databases

eggNOGCOG1218.
KOK01082.
OMAGRFWCLD.
OrthoDBEOG7VB2R7.

Enzyme and pathway databases

BioCycYEAST:YOL064C-MONOMER.

Gene expression databases

GenevestigatorP32179.

Family and domain databases

InterProIPR006239. Bisphos_HAL2.
IPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERPTHR20854. PTHR20854. 1 hit.
PfamPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSPR00377. IMPHPHTASES.
TIGRFAMsTIGR01330. bisphos_HAL2. 1 hit.
PROSITEPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32179.
NextBio975743.

Entry information

Entry nameMET22_YEAST
AccessionPrimary (citable) accession number: P32179
Secondary accession number(s): D6W203, Q6RFY5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references