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Protein

3'(2'),5'-bisphosphate nucleotidase

Gene

MET22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance. Confers resistance to lithium.

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Magnesium 11
Binding sitei72SubstrateBy similarity1
Metal bindingi142Magnesium 11
Metal bindingi142Magnesium 21
Metal bindingi144Magnesium 1; via carbonyl oxygen1
Metal bindingi145Magnesium 21
Metal bindingi294Magnesium 21
Binding sitei294SubstrateBy similarity1

GO - Molecular functioni

  • 3'(2'),5'-bisphosphate nucleotidase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • hyperosmotic salinity response Source: SGD
  • methionine biosynthetic process Source: SGD
  • phosphatidylinositol phosphorylation Source: InterPro
  • sulfate assimilation Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processStress response
LigandLithium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YOL064C-MONOMER
BRENDAi3.1.3.7 984

Names & Taxonomyi

Protein namesi
Recommended name:
3'(2'),5'-bisphosphate nucleotidase (EC:3.1.3.7)
Alternative name(s):
3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
DPNPase
Halotolerance protein HAL2
Methionine-requiring protein 22
Gene namesi
Name:MET22
Synonyms:HAL2
Ordered Locus Names:YOL064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL064C
SGDiS000005425 MET22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001425371 – 3573'(2'),5'-bisphosphate nucleotidaseAdd BLAST357

Proteomic databases

MaxQBiP32179
PaxDbiP32179
PRIDEiP32179

Expressioni

Inductioni

By salt stress.

Interactioni

Protein-protein interaction databases

BioGridi34337, 362 interactors
DIPiDIP-4072N
IntActiP32179, 2 interactors
MINTiP32179
STRINGi4932.YOL064C

Structurei

Secondary structure

1357
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 30Combined sources27
Turni31 – 34Combined sources4
Beta strandi35 – 38Combined sources4
Beta strandi44 – 46Combined sources3
Helixi47 – 63Combined sources17
Beta strandi69 – 72Combined sources4
Helixi80 – 100Combined sources21
Beta strandi111 – 113Combined sources3
Helixi118 – 126Combined sources9
Beta strandi134 – 145Combined sources12
Helixi147 – 151Combined sources5
Beta strandi157 – 164Combined sources8
Beta strandi167 – 175Combined sources9
Helixi180 – 183Combined sources4
Turni189 – 194Combined sources6
Beta strandi196 – 201Combined sources6
Turni202 – 204Combined sources3
Beta strandi206 – 210Combined sources5
Turni211 – 213Combined sources3
Beta strandi218 – 220Combined sources3
Helixi228 – 230Combined sources3
Beta strandi232 – 235Combined sources4
Turni239 – 241Combined sources3
Helixi244 – 253Combined sources10
Beta strandi258 – 261Combined sources4
Helixi266 – 273Combined sources8
Beta strandi277 – 281Combined sources5
Helixi292 – 294Combined sources3
Helixi296 – 304Combined sources9
Beta strandi308 – 310Combined sources3
Beta strandi312 – 314Combined sources3
Beta strandi322 – 325Combined sources4
Beta strandi327 – 329Combined sources3
Beta strandi331 – 335Combined sources5
Helixi338 – 353Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K9YX-ray1.90A1-357[»]
1K9ZX-ray1.50A1-357[»]
1KA0X-ray1.80A1-357[»]
1KA1X-ray1.30A1-357[»]
1QGXX-ray1.60A1-357[»]
ProteinModelPortaliP32179
SMRiP32179
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32179

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 147Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

KOiK01082
OMAiIWAIDPI
OrthoDBiEOG092C2J9G

Family and domain databases

InterProiView protein in InterPro
IPR006239 Bisphos_HAL2
IPR020583 Inositol_monoP_metal-BS
IPR000760 Inositol_monophosphatase-like
IPR020550 Inositol_monophosphatase_CS
PfamiView protein in Pfam
PF00459 Inositol_P, 1 hit
PRINTSiPR00377 IMPHPHTASES
TIGRFAMsiTIGR01330 bisphos_HAL2, 1 hit
PROSITEiView protein in PROSITE
PS00629 IMP_1, 1 hit
PS00630 IMP_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALERELLVA TQAVRKASLL TKRIQSEVIS HKDSTTITKN DNSPVTTGDY
60 70 80 90 100
AAQTIIINAI KSNFPDDKVV GEESSSGLSD AFVSGILNEI KANDEVYNKN
110 120 130 140 150
YKKDDFLFTN DQFPLKSLED VRQIIDFGNY EGGRKGRFWC LDPIDGTKGF
160 170 180 190 200
LRGEQFAVCL ALIVDGVVQL GCIGCPNLVL SSYGAQDLKG HESFGYIFRA
210 220 230 240 250
VRGLGAFYSP SSDAESWTKI HVRHLKDTKD MITLEGVEKG HSSHDEQTAI
260 270 280 290 300
KNKLNISKSL HLDSQAKYCL LALGLADVYL RLPIKLSYQE KIWDHAAGNV
310 320 330 340 350
IVHEAGGIHT DAMEDVPLDF GNGRTLATKG VIASSGPREL HDLVVSTSCD

VIQSRNA
Length:357
Mass (Da):39,149
Last modified:October 1, 1993 - v1
Checksum:iAB2E5F90B285702B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti40N → S in strain: Montrache. 1
Natural varianti61K → M in strain: Montrache. 1
Natural varianti63N → S in strain: Montrache. 1
Natural varianti308I → V in strain: Montrache. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72847 Genomic DNA Translation: CAA51361.1
AY500154 Genomic DNA Translation: AAR89916.1
Z74806 Genomic DNA Translation: CAA99074.1
BK006948 Genomic DNA Translation: DAA10719.1
PIRiS35318
RefSeqiNP_014577.1, NM_001183319.1

Genome annotation databases

EnsemblFungiiYOL064C; YOL064C; YOL064C
GeneIDi854090
KEGGisce:YOL064C

Similar proteinsi

Entry informationi

Entry nameiMET22_YEAST
AccessioniPrimary (citable) accession number: P32179
Secondary accession number(s): D6W203, Q6RFY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 20, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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