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P32178 (CHMU_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chorismate mutase

Short name=CM
EC=5.4.99.5
Gene names
Name:ARO7
Synonyms:OSM2
Ordered Locus Names:YPR060C
ORF Names:YP9499.15C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Chorismate = prephenate.

Enzyme regulation

Needs tryptophan for activation and tyrosine is a strong inhibitor. Allosterically regulated.

Pathway

Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.

Subunit structure

Homodimer.

Sequence similarities

Contains 1 chorismate mutase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256Chorismate mutase
PRO_0000119204

Regions

Domain3 – 255253Chorismate mutase

Experimental info

Mutagenesis2261T → I: Constitutively activated and feedback-resistant. Ref.1

Secondary structure

....................................... 256
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32178 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 8C6BEBEAA3497E23

FASTA25629,747
        10         20         30         40         50         60 
MDFTKPETVL NLQNIRDELV RMEDSIIFKF IERSHFATCP SVYEANHPGL EIPNFKGSFL 

        70         80         90        100        110        120 
DWALSNLEIA HSRIRRFESP DETPFFPDKI QKSFLPSINY PQILAPYAPE VNYNDKIKKV 

       130        140        150        160        170        180 
YIEKIIPLIS KRDGDDKNNF GSVATRDIEC LQSLSRRIHF GKFVAEAKFQ SDIPLYTKLI 

       190        200        210        220        230        240 
KSKDVEGIMK NITNSAVEEK ILERLTKKAE VYGVDPTNES GERRITPEYL VKIYKEIVIP 

       250 
ITKEVEVEYL LRRLEE 

« Hide

References

« Hide 'large scale' references
[1]"A single point mutation results in a constitutively activated and feedback-resistant chorismate mutase of Saccharomyces cerevisiae."
Schmidheini T., Sperisen P., Paravicini G., Huetter R., Braus G.H.
J. Bacteriol. 171:1245-1253(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF THR-226.
Strain: ATCC 26109 / X2180.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"The crystal structure of allosteric chorismate mutase at 2.2-A resolution."
Xue Y., Lipscomb W.N., Graf R., Schnappauf G., Braus G.
Proc. Natl. Acad. Sci. U.S.A. 91:10814-10818(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[7]"Crystal structure of the T state of allosteric yeast chorismate mutase and comparison with the R state."
Straeter N., Haakansson K., Schnappauf G., Braus G., Lipscomb W.N.
Proc. Natl. Acad. Sci. U.S.A. 93:3330-3334(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[8]"Mechanisms of catalysis and allosteric regulation of yeast chorismate mutase from crystal structures."
Straeter N., Schnappauf G., Braus G., Lipscomb W.N.
Structure 5:1437-1452(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24517 Genomic DNA. Translation: AAB59309.1.
Z49219 Genomic DNA. Translation: CAA89177.1.
Z71255 Genomic DNA. Translation: CAA95004.1.
AY693179 Genomic DNA. Translation: AAT93198.1.
BK006949 Genomic DNA. Translation: DAA11481.1.
PIRA45921.
RefSeqNP_015385.1. NM_001184157.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CSMX-ray2.20A/B1-256[»]
2CSMX-ray2.80A1-256[»]
3CSMX-ray3.00A/B1-256[»]
4CSMX-ray2.80A/B1-256[»]
5CSMX-ray2.00A1-256[»]
ProteinModelPortalP32178.
SMRP32178. Positions 1-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36233. 112 interactions.
IntActP32178. 3 interactions.
MINTMINT-4506098.
STRING4932.YPR060C.

Proteomic databases

MaxQBP32178.
PaxDbP32178.
PeptideAtlasP32178.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR060C; YPR060C; YPR060C.
GeneID856173.
KEGGsce:YPR060C.

Organism-specific databases

CYGDYPR060c.
SGDS000006264. ARO7.

Phylogenomic databases

eggNOGCOG1605.
HOGENOMHOG000176992.
KOK01850.
OMAFEAVEEM.
OrthoDBEOG78PVMF.

Enzyme and pathway databases

BioCycYEAST:YPR060C-MONOMER.
UniPathwayUPA00120; UER00203.

Gene expression databases

GenevestigatorP32178.

Family and domain databases

Gene3D1.10.590.10. 1 hit.
InterProIPR008238. Chorismate_mutase_AroQ_euk.
IPR020822. Chorismate_mutase_type_II.
[Graphical view]
PANTHERPTHR21145. PTHR21145. 1 hit.
PfamPF01817. CM_2. 1 hit.
[Graphical view]
PIRSFPIRSF017318. Chor_mut_AroQ_eu. 1 hit.
SUPFAMSSF48600. SSF48600. 1 hit.
TIGRFAMsTIGR01802. CM_pl-yst. 1 hit.
PROSITEPS51169. CHORISMATE_MUT_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32178.
NextBio981331.

Entry information

Entry nameCHMU_YEAST
AccessionPrimary (citable) accession number: P32178
Secondary accession number(s): D6W465
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways