Chorismate mutase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32178 (CHMU_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chorismate mutase
Short name=CM
EC=5.4.99.5

Gene names

Name:	ARO7
Synonyms:	OSM2
Ordered Locus Names:	YPR060C
ORF Names:	YP9499.15C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Chorismate = prephenate.
Enzyme regulation	Needs tryptophan for activation and tyrosine is a strong inhibitor. Allosterically regulated.
Pathway	Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Subunit structure	Homodimer.
Sequence similarities	Contains 1 chorismate mutase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Isomerase
Technical term	3D-structure Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine biosynthetic process Inferred from mutant phenotype PubMed 3027508. Source: SGD chorismate metabolic process Inferred from electronic annotation. Source: InterPro tyrosine biosynthetic process Inferred from mutant phenotype PubMed 3027508. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	chorismate mutase activity Inferred from direct assay PubMed 3027508. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 256

256

Chorismate mutase

PRO_0000119204

Regions

Domain

3 – 255

253

Chorismate mutase

Experimental info

Mutagenesis

226

T → I: Constitutively activated and feedback-resistant. Ref.1

Secondary structure

256

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32178 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 8C6BEBEAA3497E23
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FASTA

256

29,747

        10         20         30         40         50         60 
MDFTKPETVL NLQNIRDELV RMEDSIIFKF IERSHFATCP SVYEANHPGL EIPNFKGSFL 

        70         80         90        100        110        120 
DWALSNLEIA HSRIRRFESP DETPFFPDKI QKSFLPSINY PQILAPYAPE VNYNDKIKKV 

       130        140        150        160        170        180 
YIEKIIPLIS KRDGDDKNNF GSVATRDIEC LQSLSRRIHF GKFVAEAKFQ SDIPLYTKLI 

       190        200        210        220        230        240 
KSKDVEGIMK NITNSAVEEK ILERLTKKAE VYGVDPTNES GERRITPEYL VKIYKEIVIP 

       250 
ITKEVEVEYL LRRLEE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A single point mutation results in a constitutively activated and feedback-resistant chorismate mutase of Saccharomyces cerevisiae." Schmidheini T., Sperisen P., Paravicini G., Huetter R., Braus G.H. J. Bacteriol. 171:1245-1253(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF THR-226. Strain: ATCC 26109 / X2180.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The crystal structure of allosteric chorismate mutase at 2.2-A resolution." Xue Y., Lipscomb W.N., Graf R., Schnappauf G., Braus G. Proc. Natl. Acad. Sci. U.S.A. 91:10814-10818(1994) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[6]	"Crystal structure of the T state of allosteric yeast chorismate mutase and comparison with the R state." Straeter N., Haakansson K., Schnappauf G., Braus G., Lipscomb W.N. Proc. Natl. Acad. Sci. U.S.A. 93:3330-3334(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[7]	"Mechanisms of catalysis and allosteric regulation of yeast chorismate mutase from crystal structures." Straeter N., Schnappauf G., Braus G., Lipscomb W.N. Structure 5:1437-1452(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M24517 Genomic DNA. Translation: AAB59309.1.
Z49219 Genomic DNA. Translation: CAA89177.1.
Z71255 Genomic DNA. Translation: CAA95004.1.
AY693179 Genomic DNA. Translation: AAT93198.1.
BK006949 Genomic DNA. Translation: DAA11481.1.

PIR

A45921.

RefSeq

NP_015385.1. NM_001184157.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CSM	X-ray	2.20	A/B	1-256	[»]
2CSM	X-ray	2.80	A	1-256	[»]
3CSM	X-ray	3.00	A/B	1-256	[»]
4CSM	X-ray	2.80	A/B	1-256	[»]
5CSM	X-ray	2.00	A	1-256	[»]

ProteinModelPortal

P32178.

SMR

P32178. Positions 1-256.

ModBase

Search...

Protein-protein interaction databases

IntAct

P32178. 3 interactions.

STRING

4932.YPR060C.

Proteomic databases

PaxDb

P32178.

PeptideAtlas

P32178.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPR060C; YPR060C; YPR060C.

GeneID

856173.

KEGG

sce:YPR060C.

Organism-specific databases

CYGD

YPR060c.

SGD

S000006264. ARO7.

Phylogenomic databases

eggNOG

COG1605.

HOGENOM

HOG000176992.

K01850.

OMA

PDETPFF.

OrthoDB

EOG42FWT1.

Enzyme and pathway databases

UniPathway

UPA00120; UER00203.

Gene expression databases

Genevestigator

P32178.

GermOnline

YPR060C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.590.10. 1 hit.

InterPro

IPR008238. Chorismate_mutase_AroQ_euk.
IPR020822. Chorismate_mutase_type_II.
[Graphical view]

PANTHER

PTHR21145. PTHR21145. 1 hit.

Pfam

PF01817. CM_2. 1 hit.
[Graphical view]

PIRSF

PIRSF017318. Chor_mut_AroQ_eu. 1 hit.

SUPFAM

SSF48600. Chorismate_mut. 1 hit.

TIGRFAMs

TIGR01802. CM_pl-yst. 1 hit.

PROSITE

PS51169. CHORISMATE_MUT_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P32178.

NextBio

981331.

Entry information

Entry name

CHMU_YEAST

Accession

Primary (citable) accession number: P32178
Secondary accession number(s): D6W465

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents