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P32176

- FDOG_ECOLI

UniProt

P32176 - FDOG_ECOLI

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Protein

Formate dehydrogenase-O major subunit

Gene
fdoG, b3894, JW3865
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.
Binds 1 4Fe-4S cluster Reviewed prediction.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarity
Metal bindingi57 – 571Iron-sulfur (4Fe-4S) By similarity
Metal bindingi92 – 921Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  4. molybdenum ion binding Source: InterPro
  5. molybdopterin cofactor binding Source: EcoCyc
  6. oxidoreductase activity Source: RefGenome
  7. protein binding Source: IntAct

GO - Biological processi

  1. anaerobic respiration Source: EcoCyc
  2. cellular respiration Source: EcoCyc
  3. cellular response to DNA damage stimulus Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciEcoCyc:FDOG-MONOMER.
ECOL316407:JW3865-MONOMER.
MetaCyc:FDOG-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase-O major subunit (EC:1.2.1.2)
Alternative name(s):
Aerobic formate dehydrogenase major subunit
FDH-Z subunit alpha
Formate dehydrogenase-O subunit alpha
Gene namesi
Name:fdoG
Ordered Locus Names:b3894, JW3865
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11858. fdoG.

Subcellular locationi

Periplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: EcoCyc
  2. membrane Source: UniProtKB
  3. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signal Reviewed predictionAdd
BLAST
Chaini34 – 1016983Formate dehydrogenase-O major subunitPRO_0000063223Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP32176.
PRIDEiP32176.

Expressioni

Gene expression databases

GenevestigatoriP32176.

Interactioni

Subunit structurei

Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

Binary interactionsi

WithEntry#Exp.IntActNotes
fdhEP130243EBI-368676,EBI-550129

Protein-protein interaction databases

BioGridi852691. 1 interaction.
DIPiDIP-9576N.
IntActiP32176. 12 interactions.
MINTiMINT-8047511.
STRINGi511145.b3894.

Structurei

3D structure databases

ProteinModelPortaliP32176.
SMRiP32176. Positions 34-1015.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 106644Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000163271.
KOiK00123.
OMAiNYSTPHE.
OrthoDBiEOG6D8B5R.
PhylomeDBiP32176.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 2 hits.
TIGRFAMsiTIGR01553. formate-DH-alph. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32176-1 [UniParc]FASTAAdd to Basket

« Hide

MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC     50
TYCSVGCGLL MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD 100
FIHSESRLKF PEYRAPGSDK WQQISWEEAF DRIAKLMKED RDANYIAQNA 150
EGVTVNRWLS TGMLCASASS NETGYLTQKF SRALGMLAVD NQARVUHGPT 200
VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG FRWAMEAKIH 250
NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE 300
YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR 350
DTTLQHPRCV WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH 400
DKTASFLYAL GWTQHSVGAQ NIRTMAMIQL LLGNMGMAGG GVNALRGHSN 450
IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT YLTANTPKPL LEGQVNYWGN 500
YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY FEMMKEGKVN 550
GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN 600
EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG 650
EILSGIFLRL RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK 700
ALADITDPAT GAVIVKKGQQ LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ 750
MARRDNADPS GLGNTLGWAW AWPLNRRILY NRASADPQGN PWDPKRQLLK 800
WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD KMAEGPFPEH 850
YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE 900
HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA 950
KAVVTKRIRT LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN 1000
TQTPEFKSFL VNVEKV 1016
Length:1,016
Mass (Da):112,549
Last modified:February 26, 2008 - v5
Checksum:iDED0952028055769
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 26110GAKLIVIDPR → RREADCDRSC in AAB03027. 1 Publication
Sequence conflicti344 – 3485ENGFA → GKRLR in AAB03027. 1 Publication

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei196 – 1961Selenocysteine

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19201 Genomic DNA. Translation: AAB03027.2.
U00096 Genomic DNA. Translation: AAD13456.1.
AP009048 Genomic DNA. Translation: BAE77415.1.
X87583 Genomic DNA. Translation: CAA60887.1.
PIRiA65195. S40838.
RefSeqiNP_418330.1. NC_000913.3.
YP_491556.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAD13456; AAD13456; b3894.
BAE77415; BAE77415; BAE77415.
GeneIDi12934329.
948394.
KEGGiecj:Y75_p3292.
eco:b3894.
PATRICi32123295. VBIEscCol129921_4008.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19201 Genomic DNA. Translation: AAB03027.2 .
U00096 Genomic DNA. Translation: AAD13456.1 .
AP009048 Genomic DNA. Translation: BAE77415.1 .
X87583 Genomic DNA. Translation: CAA60887.1 .
PIRi A65195. S40838.
RefSeqi NP_418330.1. NC_000913.3.
YP_491556.1. NC_007779.1.

3D structure databases

ProteinModelPortali P32176.
SMRi P32176. Positions 34-1015.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 852691. 1 interaction.
DIPi DIP-9576N.
IntActi P32176. 12 interactions.
MINTi MINT-8047511.
STRINGi 511145.b3894.

Proteomic databases

PaxDbi P32176.
PRIDEi P32176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD13456 ; AAD13456 ; b3894 .
BAE77415 ; BAE77415 ; BAE77415 .
GeneIDi 12934329.
948394.
KEGGi ecj:Y75_p3292.
eco:b3894.
PATRICi 32123295. VBIEscCol129921_4008.

Organism-specific databases

EchoBASEi EB1804.
EcoGenei EG11858. fdoG.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000163271.
KOi K00123.
OMAi NYSTPHE.
OrthoDBi EOG6D8B5R.
PhylomeDBi P32176.

Enzyme and pathway databases

BioCyci EcoCyc:FDOG-MONOMER.
ECOL316407:JW3865-MONOMER.
MetaCyc:FDOG-MONOMER.

Miscellaneous databases

PROi P32176.

Gene expression databases

Genevestigatori P32176.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 2 hits.
TIGRFAMsi TIGR01553. formate-DH-alph. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase."
    Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A.
    J. Bacteriol. 177:7141-7149(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION.
    Strain: K12.
  5. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM.

Entry informationi

Entry nameiFDOG_ECOLI
AccessioniPrimary (citable) accession number: P32176
Secondary accession number(s): P78131, Q2M8J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 26, 2008
Last modified: June 11, 2014
This is version 139 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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