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P32176

- FDOG_ECOLI

UniProt

P32176 - FDOG_ECOLI

Protein

Formate dehydrogenase-O major subunit

Gene

fdoG

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 5 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.

    Catalytic activityi

    Formate + NAD+ = CO2 + NADH.

    Cofactori

    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity
    Binds 1 4Fe-4S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi57 – 571Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi92 – 921Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    4. molybdenum ion binding Source: InterPro
    5. molybdopterin cofactor binding Source: EcoCyc
    6. oxidoreductase activity Source: RefGenome
    7. protein binding Source: IntAct

    GO - Biological processi

    1. anaerobic respiration Source: EcoCyc
    2. cellular respiration Source: EcoCyc
    3. cellular response to DNA damage stimulus Source: EcoliWiki

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:FDOG-MONOMER.
    ECOL316407:JW3865-MONOMER.
    MetaCyc:FDOG-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate dehydrogenase-O major subunit (EC:1.2.1.2)
    Alternative name(s):
    Aerobic formate dehydrogenase major subunit
    FDH-Z subunit alpha
    Formate dehydrogenase-O subunit alpha
    Gene namesi
    Name:fdoG
    Ordered Locus Names:b3894, JW3865
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11858. fdoG.

    Subcellular locationi

    Periplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: EcoCyc
    2. membrane Source: UniProtKB
    3. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Tat-type signalPROSITE-ProRule annotationAdd
    BLAST
    Chaini34 – 1016983Formate dehydrogenase-O major subunitPRO_0000063223Add
    BLAST

    Post-translational modificationi

    Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Proteomic databases

    PaxDbiP32176.
    PRIDEiP32176.

    Expressioni

    Gene expression databases

    GenevestigatoriP32176.

    Interactioni

    Subunit structurei

    Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    fdhEP130243EBI-368676,EBI-550129

    Protein-protein interaction databases

    BioGridi852691. 1 interaction.
    DIPiDIP-9576N.
    IntActiP32176. 12 interactions.
    MINTiMINT-8047511.
    STRINGi511145.b3894.

    Structurei

    3D structure databases

    ProteinModelPortaliP32176.
    SMRiP32176. Positions 34-1015.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 106644Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000163271.
    KOiK00123.
    OMAiNYSTPHE.
    OrthoDBiEOG6D8B5R.
    PhylomeDBiP32176.

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006443. Formate_DH_asu_anaerob.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 2 hits.
    TIGRFAMsiTIGR01553. formate-DH-alph. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32176-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC     50
    TYCSVGCGLL MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD 100
    FIHSESRLKF PEYRAPGSDK WQQISWEEAF DRIAKLMKED RDANYIAQNA 150
    EGVTVNRWLS TGMLCASASS NETGYLTQKF SRALGMLAVD NQARVUHGPT 200
    VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG FRWAMEAKIH 250
    NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE 300
    YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR 350
    DTTLQHPRCV WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH 400
    DKTASFLYAL GWTQHSVGAQ NIRTMAMIQL LLGNMGMAGG GVNALRGHSN 450
    IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT YLTANTPKPL LEGQVNYWGN 500
    YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY FEMMKEGKVN 550
    GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN 600
    EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG 650
    EILSGIFLRL RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK 700
    ALADITDPAT GAVIVKKGQQ LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ 750
    MARRDNADPS GLGNTLGWAW AWPLNRRILY NRASADPQGN PWDPKRQLLK 800
    WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD KMAEGPFPEH 850
    YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE 900
    HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA 950
    KAVVTKRIRT LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN 1000
    TQTPEFKSFL VNVEKV 1016
    Length:1,016
    Mass (Da):112,549
    Last modified:February 26, 2008 - v5
    Checksum:iDED0952028055769
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti252 – 26110GAKLIVIDPR → RREADCDRSC in AAB03027. (PubMed:8346018)Curated
    Sequence conflicti344 – 3485ENGFA → GKRLR in AAB03027. (PubMed:8346018)Curated

    Non-standard residue

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-standard residuei196 – 1961Selenocysteine

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19201 Genomic DNA. Translation: AAB03027.2.
    U00096 Genomic DNA. Translation: AAD13456.1.
    AP009048 Genomic DNA. Translation: BAE77415.1.
    X87583 Genomic DNA. Translation: CAA60887.1.
    PIRiA65195. S40838.
    RefSeqiNP_418330.1. NC_000913.3.
    YP_491556.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAD13456; AAD13456; b3894.
    BAE77415; BAE77415; BAE77415.
    GeneIDi12934329.
    948394.
    KEGGiecj:Y75_p3292.
    eco:b3894.
    PATRICi32123295. VBIEscCol129921_4008.

    Keywords - Coding sequence diversityi

    Selenocysteine

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19201 Genomic DNA. Translation: AAB03027.2 .
    U00096 Genomic DNA. Translation: AAD13456.1 .
    AP009048 Genomic DNA. Translation: BAE77415.1 .
    X87583 Genomic DNA. Translation: CAA60887.1 .
    PIRi A65195. S40838.
    RefSeqi NP_418330.1. NC_000913.3.
    YP_491556.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P32176.
    SMRi P32176. Positions 34-1015.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 852691. 1 interaction.
    DIPi DIP-9576N.
    IntActi P32176. 12 interactions.
    MINTi MINT-8047511.
    STRINGi 511145.b3894.

    Proteomic databases

    PaxDbi P32176.
    PRIDEi P32176.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD13456 ; AAD13456 ; b3894 .
    BAE77415 ; BAE77415 ; BAE77415 .
    GeneIDi 12934329.
    948394.
    KEGGi ecj:Y75_p3292.
    eco:b3894.
    PATRICi 32123295. VBIEscCol129921_4008.

    Organism-specific databases

    EchoBASEi EB1804.
    EcoGenei EG11858. fdoG.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000163271.
    KOi K00123.
    OMAi NYSTPHE.
    OrthoDBi EOG6D8B5R.
    PhylomeDBi P32176.

    Enzyme and pathway databases

    BioCyci EcoCyc:FDOG-MONOMER.
    ECOL316407:JW3865-MONOMER.
    MetaCyc:FDOG-MONOMER.

    Miscellaneous databases

    PROi P32176.

    Gene expression databases

    Genevestigatori P32176.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006443. Formate_DH_asu_anaerob.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 2 hits.
    TIGRFAMsi TIGR01553. formate-DH-alph. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
      Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822.
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase."
      Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A.
      J. Bacteriol. 177:7141-7149(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION.
      Strain: K12.
    5. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
      Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
      J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: EXPORT VIA THE TAT-SYSTEM.

    Entry informationi

    Entry nameiFDOG_ECOLI
    AccessioniPrimary (citable) accession number: P32176
    Secondary accession number(s): P78131, Q2M8J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 140 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3