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P32176 (FDOG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate dehydrogenase-O major subunit

EC=1.2.1.2
Alternative name(s):
Aerobic formate dehydrogenase major subunit
FDH-Z subunit alpha
Formate dehydrogenase-O subunit alpha
Gene names
Name:fdoG
Ordered Locus Names:b3894, JW3865
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length1016 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.

Catalytic activity

Formate + NAD+ = CO2 + NADH.

Cofactor

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Binds 1 4Fe-4S cluster Potential.

Subunit structure

Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

Subcellular location

Periplasm Potential.

Post-translational modification

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

fdhEP130243EBI-368676,EBI-550129

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333Tat-type signal Potential
Chain34 – 1016983Formate dehydrogenase-O major subunit
PRO_0000063223

Regions

Domain43 – 106644Fe-4S Mo/W bis-MGD-type

Sites

Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding571Iron-sulfur (4Fe-4S) By similarity
Metal binding921Iron-sulfur (4Fe-4S) By similarity

Amino acid modifications

Non-standard residue1961Selenocysteine

Experimental info

Sequence conflict252 – 26110GAKLIVIDPR → RREADCDRSC in AAB03027. Ref.1
Sequence conflict344 – 3485ENGFA → GKRLR in AAB03027. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32176 [UniParc].

Last modified February 26, 2008. Version 5.
Checksum: DED0952028055769

FASTA1,016112,549
        10         20         30         40         50         60 
MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC TYCSVGCGLL 

        70         80         90        100        110        120 
MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD FIHSESRLKF PEYRAPGSDK 

       130        140        150        160        170        180 
WQQISWEEAF DRIAKLMKED RDANYIAQNA EGVTVNRWLS TGMLCASASS NETGYLTQKF 

       190        200        210        220        230        240 
SRALGMLAVD NQARVUHGPT VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG 

       250        260        270        280        290        300 
FRWAMEAKIH NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE 

       310        320        330        340        350        360 
YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR DTTLQHPRCV 

       370        380        390        400        410        420 
WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH DKTASFLYAL GWTQHSVGAQ 

       430        440        450        460        470        480 
NIRTMAMIQL LLGNMGMAGG GVNALRGHSN IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT 

       490        500        510        520        530        540 
YLTANTPKPL LEGQVNYWGN YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY 

       550        560        570        580        590        600 
FEMMKEGKVN GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN 

       610        620        630        640        650        660 
EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG EILSGIFLRL 

       670        680        690        700        710        720 
RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK ALADITDPAT GAVIVKKGQQ 

       730        740        750        760        770        780 
LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ MARRDNADPS GLGNTLGWAW AWPLNRRILY 

       790        800        810        820        830        840 
NRASADPQGN PWDPKRQLLK WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD 

       850        860        870        880        890        900 
KMAEGPFPEH YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE 

       910        920        930        940        950        960 
HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA KAVVTKRIRT 

       970        980        990       1000       1010 
LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN TQTPEFKSFL VNVEKV 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822.
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase."
Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A.
J. Bacteriol. 177:7141-7149(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION.
Strain: K12.
[5]"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPORT VIA THE TAT-SYSTEM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19201 Genomic DNA. Translation: AAB03027.2.
U00096 Genomic DNA. Translation: AAD13456.1.
AP009048 Genomic DNA. Translation: BAE77415.1.
X87583 Genomic DNA. Translation: CAA60887.1.
PIRS40838. A65195.
RefSeqNP_418330.1. NC_000913.3.
YP_491556.1. NC_007779.1.

3D structure databases

ProteinModelPortalP32176.
SMRP32176. Positions 34-1015.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid852691. 1 interaction.
DIPDIP-9576N.
IntActP32176. 12 interactions.
MINTMINT-8047511.
STRING511145.b3894.

Proteomic databases

PaxDbP32176.
PRIDEP32176.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD13456; AAD13456; b3894.
BAE77415; BAE77415; BAE77415.
GeneID12934329.
948394.
KEGGecj:Y75_p3292.
eco:b3894.
PATRIC32123295. VBIEscCol129921_4008.

Organism-specific databases

EchoBASEEB1804.
EcoGeneEG11858. fdoG.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000163271.
KOK00123.
OMANYSTPHE.
OrthoDBEOG6D8B5R.
PhylomeDBP32176.

Enzyme and pathway databases

BioCycEcoCyc:FDOG-MONOMER.
ECOL316407:JW3865-MONOMER.
MetaCyc:FDOG-MONOMER.

Gene expression databases

GenevestigatorP32176.

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 2 hits.
TIGRFAMsTIGR01553. formate-DH-alph. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP32176.

Entry information

Entry nameFDOG_ECOLI
AccessionPrimary (citable) accession number: P32176
Secondary accession number(s): P78131, Q2M8J1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 26, 2008
Last modified: June 11, 2014
This is version 139 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene