Reviewed,
UniProtKB/Swiss-Prot P32176 (FDOG_ECOLI)
Last modified
June 16, 2009.
Version 90.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Formate dehydrogenase-O major subunit EC=1.2.1.2 Alternative name(s): Formate dehydrogenase-O subunit alpha FDH-Z subunit alpha Aerobic formate dehydrogenase major subunit | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 1016 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site. |
| Catalytic activity | Formate + NAD+ = CO2 + NADH. |
| Cofactor | Molybdenum (molybdopterin). Binds 1 4Fe-4S cluster Potential. |
| Subunit structure | Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma. |
| Subcellular location | Periplasm Potential. |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
| Sequence similarities | Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 33 | 33 | Tat-type signal Potential | ||||||
| Chain | 34 – 1016 | 983 | Formate dehydrogenase-O major subunit | PRO_0000063223 | |||||
Sites | |||||||||
| Metal binding | 50 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 53 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 57 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 92 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Amino acid modifications | |||||||||
| Non-standard residue | 196 | 1 | Selenocysteine | ||||||
Experimental info | |||||||||
| Sequence conflict | 252 – 261 | 10 | GAKLIVIDPR → RREADCDRSC in AAB03027. Ref.1 | ||||||
| Sequence conflict | 344 – 348 | 5 | ENGFA → GKRLR in AAB03027. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase." Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A. J. Bacteriol. 177:7141-7149(1995) [PubMed: 8522521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION. Strain: K12. |
Cross-references
Sequence databases | |
|---|---|
| L19201 Genomic DNA. Translation: AAB03027.2. U00096 Genomic DNA. Translation: AAD13456.1. AP009048 Genomic DNA. Translation: BAE77415.1. X87583 Genomic DNA. Translation: CAA60887.1. | |
| PIR | S40838. A65195. |
| RefSeq | AP_003914.1. NP_418330.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1KQF based on UniProtKB P24183. |
| SMR | P32176. Positions 34-1015. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P32176. 12 interactions. |
Genome annotation databases | |
| GeneID | 948394. |
| GenomeReviews | Gene locus JW3865 in contig AP009048_GR. Gene locus b3894 in contig U00096_GR. |
| KEGG | ecj:JW3865. eco:b3894. |
Organism-specific databases | |
| EchoBASE | EB1804. |
| EcoGene | EG11858. fdoG. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P32176. |
| OMA | P32176. TEHFHFW. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:FDOG-MON. MetaCyc:FDOG-MON. |
Family and domain databases | |
| InterPro | IPR009010. Asp_de-COase-like_fold. IPR006443. Formate_DH_asu_anaerob. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view] |
| Gene3D | G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit. |
| Pfam | PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01553. formate-DH-alph. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FDOG_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P32176 Secondary accession number(s): P78131, Q2M8J1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


