Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32176

- FDOG_ECOLI

UniProt

P32176 - FDOG_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Formate dehydrogenase-O major subunit

Gene

fdoG

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi57 – 571Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi92 – 921Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  4. molybdenum ion binding Source: InterPro
  5. molybdopterin cofactor binding Source: EcoCyc
  6. oxidoreductase activity Source: RefGenome

GO - Biological processi

  1. anaerobic respiration Source: EcoCyc
  2. cellular respiration Source: EcoCyc
  3. cellular response to DNA damage stimulus Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciEcoCyc:FDOG-MONOMER.
ECOL316407:JW3865-MONOMER.
MetaCyc:FDOG-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase-O major subunit (EC:1.2.1.2)
Alternative name(s):
Aerobic formate dehydrogenase major subunit
FDH-Z subunit alpha
Formate dehydrogenase-O subunit alpha
Gene namesi
Name:fdoG
Ordered Locus Names:b3894, JW3865
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11858. fdoG.

Subcellular locationi

Periplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: EcoCyc
  2. membrane Source: UniProtKB
  3. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini34 – 1016983Formate dehydrogenase-O major subunitPRO_0000063223Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP32176.
PRIDEiP32176.

Expressioni

Gene expression databases

GenevestigatoriP32176.

Interactioni

Subunit structurei

Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

Binary interactionsi

WithEntry#Exp.IntActNotes
fdhEP130243EBI-368676,EBI-550129

Protein-protein interaction databases

BioGridi852691. 1 interaction.
DIPiDIP-9576N.
IntActiP32176. 12 interactions.
MINTiMINT-8047511.
STRINGi511145.b3894.

Structurei

3D structure databases

ProteinModelPortaliP32176.
SMRiP32176. Positions 34-1015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 106644Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000163271.
InParanoidiP32176.
KOiK00123.
OMAiNYSTPHE.
OrthoDBiEOG6D8B5R.
PhylomeDBiP32176.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 2 hits.
TIGRFAMsiTIGR01553. formate-DH-alph. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32176-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC
60 70 80 90 100
TYCSVGCGLL MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD
110 120 130 140 150
FIHSESRLKF PEYRAPGSDK WQQISWEEAF DRIAKLMKED RDANYIAQNA
160 170 180 190 200
EGVTVNRWLS TGMLCASASS NETGYLTQKF SRALGMLAVD NQARVUHGPT
210 220 230 240 250
VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG FRWAMEAKIH
260 270 280 290 300
NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE
310 320 330 340 350
YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR
360 370 380 390 400
DTTLQHPRCV WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH
410 420 430 440 450
DKTASFLYAL GWTQHSVGAQ NIRTMAMIQL LLGNMGMAGG GVNALRGHSN
460 470 480 490 500
IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT YLTANTPKPL LEGQVNYWGN
510 520 530 540 550
YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY FEMMKEGKVN
560 570 580 590 600
GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN
610 620 630 640 650
EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG
660 670 680 690 700
EILSGIFLRL RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK
710 720 730 740 750
ALADITDPAT GAVIVKKGQQ LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ
760 770 780 790 800
MARRDNADPS GLGNTLGWAW AWPLNRRILY NRASADPQGN PWDPKRQLLK
810 820 830 840 850
WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD KMAEGPFPEH
860 870 880 890 900
YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE
910 920 930 940 950
HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA
960 970 980 990 1000
KAVVTKRIRT LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN
1010
TQTPEFKSFL VNVEKV
Length:1,016
Mass (Da):112,549
Last modified:February 26, 2008 - v5
Checksum:iDED0952028055769
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 26110GAKLIVIDPR → RREADCDRSC in AAB03027. (PubMed:8346018)Curated
Sequence conflicti344 – 3485ENGFA → GKRLR in AAB03027. (PubMed:8346018)Curated

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei196 – 1961Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03027.2.
U00096 Genomic DNA. Translation: AAD13456.1.
AP009048 Genomic DNA. Translation: BAE77415.1.
X87583 Genomic DNA. Translation: CAA60887.1.
PIRiA65195. S40838.
RefSeqiNP_418330.1. NC_000913.3.
YP_491556.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAD13456; AAD13456; b3894.
BAE77415; BAE77415; BAE77415.
GeneIDi12934329.
948394.
KEGGiecj:Y75_p3292.
eco:b3894.
PATRICi32123295. VBIEscCol129921_4008.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03027.2 .
U00096 Genomic DNA. Translation: AAD13456.1 .
AP009048 Genomic DNA. Translation: BAE77415.1 .
X87583 Genomic DNA. Translation: CAA60887.1 .
PIRi A65195. S40838.
RefSeqi NP_418330.1. NC_000913.3.
YP_491556.1. NC_007779.1.

3D structure databases

ProteinModelPortali P32176.
SMRi P32176. Positions 34-1015.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 852691. 1 interaction.
DIPi DIP-9576N.
IntActi P32176. 12 interactions.
MINTi MINT-8047511.
STRINGi 511145.b3894.

Proteomic databases

PaxDbi P32176.
PRIDEi P32176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD13456 ; AAD13456 ; b3894 .
BAE77415 ; BAE77415 ; BAE77415 .
GeneIDi 12934329.
948394.
KEGGi ecj:Y75_p3292.
eco:b3894.
PATRICi 32123295. VBIEscCol129921_4008.

Organism-specific databases

EchoBASEi EB1804.
EcoGenei EG11858. fdoG.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000163271.
InParanoidi P32176.
KOi K00123.
OMAi NYSTPHE.
OrthoDBi EOG6D8B5R.
PhylomeDBi P32176.

Enzyme and pathway databases

BioCyci EcoCyc:FDOG-MONOMER.
ECOL316407:JW3865-MONOMER.
MetaCyc:FDOG-MONOMER.

Miscellaneous databases

PROi P32176.

Gene expression databases

Genevestigatori P32176.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 2 hits.
TIGRFAMsi TIGR01553. formate-DH-alph. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase."
    Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A.
    J. Bacteriol. 177:7141-7149(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION.
    Strain: K12.
  5. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM.

Entry informationi

Entry nameiFDOG_ECOLI
AccessioniPrimary (citable) accession number: P32176
Secondary accession number(s): P78131, Q2M8J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 26, 2008
Last modified: November 26, 2014
This is version 142 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3