Reviewed, UniProtKB/Swiss-Prot P32176 (FDOG_ECOLI)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase-O major subunit
    EC=1.2.1.2
Alternative name(s):
    Formate dehydrogenase-O subunit alpha
    FDH-Z subunit alpha
    Aerobic formate dehydrogenase major subunit

Gene names

Name:	fdoG
Ordered Locus Names:	b3894, JW3865

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	1016 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.
Catalytic activity	Formate + NAD+ = CO2 + NADH.
Cofactor	Molybdenum (molybdopterin). Binds 1 4Fe-4S cluster Potential.
Subunit structure	Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.
Subcellular location	Periplasm Potential.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Coding sequence diversity	Selenocysteine
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum NAD Selenium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular respiration Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro membrane Inferred from direct assay. Source: UniProtKB periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
udp	P12758	1	EBI-368676,EBI-370246

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Tat-type signal Potential
	Chain	34 – 1016	983	Formate dehydrogenase-O major subunit		PRO_0000063223
Sites
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	57	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	92	1	Iron-sulfur (4Fe-4S) By similarity
Amino acid modifications
	Non-standard residue	196	1	Selenocysteine
Experimental info
	Sequence conflict	252 – 261	10	GAKLIVIDPR → RREADCDRSC in AAB03027. Ref.1
	Sequence conflict	344 – 348	5	ENGFA → GKRLR in AAB03027. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32176-1 [UniParc]. Last modified February 26, 2008. Version 5. Checksum: DED0952028055769	FASTA	1,016	112,549
	10 20 30 40 50 60 MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC TYCSVGCGLL 70 80 90 100 110 120 MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD FIHSESRLKF PEYRAPGSDK 130 140 150 160 170 180 WQQISWEEAF DRIAKLMKED RDANYIAQNA EGVTVNRWLS TGMLCASASS NETGYLTQKF 190 200 210 220 230 240 SRALGMLAVD NQARVUHGPT VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG 250 260 270 280 290 300 FRWAMEAKIH NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE 310 320 330 340 350 360 YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR DTTLQHPRCV 370 380 390 400 410 420 WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH DKTASFLYAL GWTQHSVGAQ 430 440 450 460 470 480 NIRTMAMIQL LLGNMGMAGG GVNALRGHSN IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT 490 500 510 520 530 540 YLTANTPKPL LEGQVNYWGN YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY 550 560 570 580 590 600 FEMMKEGKVN GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN 610 620 630 640 650 660 EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG EILSGIFLRL 670 680 690 700 710 720 RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK ALADITDPAT GAVIVKKGQQ 730 740 750 760 770 780 LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ MARRDNADPS GLGNTLGWAW AWPLNRRILY 790 800 810 820 830 840 NRASADPQGN PWDPKRQLLK WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD 850 860 870 880 890 900 KMAEGPFPEH YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE 910 920 930 940 950 960 HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA KAVVTKRIRT 970 980 990 1000 1010 LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN TQTPEFKSFL VNVEKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase." Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A. J. Bacteriol. 177:7141-7149(1995) [PubMed: 8522521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19201 Genomic DNA. Translation: AAB03027.2. U00096 Genomic DNA. Translation: AAD13456.1. AP009048 Genomic DNA. Translation: BAE77415.1. X87583 Genomic DNA. Translation: CAA60887.1.
PIR	S40838. A65195.
RefSeq	AP_003914.1. NP_418330.1.
3D structure databases
HSSP	HSSP built from PDB template 1KQF based on UniProtKB P24183.
SMR	P32176. Positions 34-1015.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32176. 12 interactions.
Genome annotation databases
GeneID	948394.
GenomeReviews	Gene locus JW3865 in contig AP009048_GR. Gene locus b3894 in contig U00096_GR.
KEGG	ecj:JW3865. eco:b3894.
Organism-specific databases
EchoBASE	EB1804.
EcoGene	EG11858. fdoG.
CMR	Search...
Phylogenomic databases
HOGENOM	P32176.
OMA	P32176. TEHFHFW.
Enzyme and pathway databases
BioCyc	EcoCyc:FDOG-MON. MetaCyc:FDOG-MON.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006443. Formate_DH_asu_anaerob. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01553. formate-DH-alph. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDOG_ECOLI

Accession

Primary (citable) accession number: P32176
Secondary accession number(s): P78131, Q2M8J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 90 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)