Formate dehydrogenase-O major subunit precursor - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P32176 (FDOG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 129. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Formate dehydrogenase-O major subunit
EC=1.2.1.2

Alternative name(s):
Aerobic formate dehydrogenase major subunit
FDH-Z subunit alpha
Formate dehydrogenase-O subunit alpha

Gene names

Name:	fdoG
Ordered Locus Names:	b3894, JW3865

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	1016 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.
Catalytic activity	Formate + NAD⁺ = CO₂ + NADH.
Cofactor	Molybdenum (molybdopterin). Binds 1 4Fe-4S cluster Potential.
Subunit structure	Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.
Subcellular location	Periplasm Potential.
Post-translational modification	Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Coding sequence diversity	Selenocysteine
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anaerobic respiration Inferred from mutant phenotype PubMed 1504073. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	cytoplasm Inferred from direct assay PubMed 9852007. Source: EcoCyc membrane Inferred from direct assay PubMed 16858726. Source: UniProtKB periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC molybdenum ion binding Inferred from electronic annotation. Source: InterPro molybdopterin cofactor binding Inferred from direct assay PubMed 1504073. Source: EcoCyc oxidoreductase activity Inferred from Biological aspect of Ancestor. Source: RefGenome oxidoreductase activity, acting on other nitrogenous compounds as donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 33

Tat-type signal Potential

Chain

34 – 1016

983

Formate dehydrogenase-O major subunit

PRO_0000063223

Sites

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Amino acid modifications

Non-standard residue

196

Selenocysteine

Experimental info

Sequence conflict

252 – 261

GAKLIVIDPR → RREADCDRSC in AAB03027. Ref.1

Sequence conflict

344 – 348

ENGFA → GKRLR in AAB03027. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P32176 [UniParc].

Last modified February 26, 2008. Version 5.
Checksum: DED0952028055769
Show »

FASTA

1,016

112,549

        10         20         30         40         50         60 
MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC TYCSVGCGLL 

        70         80         90        100        110        120 
MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD FIHSESRLKF PEYRAPGSDK 

       130        140        150        160        170        180 
WQQISWEEAF DRIAKLMKED RDANYIAQNA EGVTVNRWLS TGMLCASASS NETGYLTQKF 

       190        200        210        220        230        240 
SRALGMLAVD NQARVUHGPT VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG 

       250        260        270        280        290        300 
FRWAMEAKIH NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE 

       310        320        330        340        350        360 
YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR DTTLQHPRCV 

       370        380        390        400        410        420 
WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH DKTASFLYAL GWTQHSVGAQ 

       430        440        450        460        470        480 
NIRTMAMIQL LLGNMGMAGG GVNALRGHSN IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT 

       490        500        510        520        530        540 
YLTANTPKPL LEGQVNYWGN YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY 

       550        560        570        580        590        600 
FEMMKEGKVN GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN 

       610        620        630        640        650        660 
EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG EILSGIFLRL 

       670        680        690        700        710        720 
RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK ALADITDPAT GAVIVKKGQQ 

       730        740        750        760        770        780 
LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ MARRDNADPS GLGNTLGWAW AWPLNRRILY 

       790        800        810        820        830        840 
NRASADPQGN PWDPKRQLLK WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD 

       850        860        870        880        890        900 
KMAEGPFPEH YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE 

       910        920        930        940        950        960 
HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA KAVVTKRIRT 

       970        980        990       1000       1010 
LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN TQTPEFKSFL VNVEKV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 252-261; 344-348 AND 822. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase." Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A. J. Bacteriol. 177:7141-7149(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, CHARACTERIZATION. Strain: K12.
[5]	"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G. J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract] Cited for: EXPORT VIA THE TAT-SYSTEM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19201 Genomic DNA. Translation: AAB03027.2. U00096 Genomic DNA. Translation: AAD13456.1. AP009048 Genomic DNA. Translation: BAE77415.1. X87583 Genomic DNA. Translation: CAA60887.1.
PIR	S40838. A65195.
RefSeq	NP_418330.1. NC_000913.2. YP_491556.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P32176.
SMR	P32176. Positions 34-1015.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9576N.
IntAct	P32176. 12 interactions.
STRING	511145.b3894.
Proteomic databases
PaxDb	P32176.
PRIDE	P32176.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD13456; AAD13456; b3894. BAE77415; BAE77415; BAE77415.
GeneID	12934329. 948394.
KEGG	ecj:Y75_p3292. eco:b3894.
PATRIC	32123295. VBIEscCol129921_4008.
Organism-specific databases
EchoBASE	EB1804.
EcoGene	EG11858. fdoG.
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000163271.
KO	K00123.
OMA	QYFEMMN.
ProtClustDB	CLSK880048.
Enzyme and pathway databases
BioCyc	EcoCyc:FDOG-MONOMER. ECOL316407:JW3865-MONOMER. MetaCyc:FDOG-MONOMER.
Gene expression databases
Genevestigator	P32176.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006443. Formate_DH_asu_anaerob. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006311. TAT_signal. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01553. formate-DH-alph. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDOG_ECOLI

Accession

Primary (citable) accession number: P32176
Secondary accession number(s): P78131, Q2M8J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 129 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents