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Protein

Formate dehydrogenase-O major subunit

Gene

fdoG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi57Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi92Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellular respiration Source: EcoCyc
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • formate oxidation Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciEcoCyc:FDOG-MONOMER
MetaCyc:FDOG-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase-O major subunit (EC:1.17.1.9)
Alternative name(s):
Aerobic formate dehydrogenase major subunit
FDH-Z subunit alpha
Formate dehydrogenase-O subunit alpha
Gene namesi
Name:fdoG
Ordered Locus Names:b3894, JW3865
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11858 fdoG

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • formate dehydrogenase complex Source: EcoCyc
  • membrane Source: UniProtKB
  • periplasmic space Source: UniProtKB-SubCell

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Tat-type signalPROSITE-ProRule annotationAdd BLAST33
ChainiPRO_000006322334 – 1016Formate dehydrogenase-O major subunitAdd BLAST983

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

EPDiP32176
PaxDbiP32176
PRIDEiP32176

Interactioni

Subunit structurei

Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

Binary interactionsi

WithEntry#Exp.IntActNotes
fdhEP130244EBI-368676,EBI-550129

Protein-protein interaction databases

BioGridi426332336 interactors.
8526911 interactor.
DIPiDIP-9576N
IntActiP32176 12 interactors.
MINTiP32176
STRINGi316385.ECDH10B_4084

Structurei

3D structure databases

ProteinModelPortaliP32176
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 1064Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST64

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000163271
InParanoidiP32176
KOiK00123
OMAiQNIRSMA
PhylomeDBiP32176

Family and domain databases

InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006443 Formate-DH-alph_fdnG
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR006655 Mopterin_OxRdtase_prok_CS
IPR027467 MopterinOxRdtase_cofactor_BS
IPR006311 TAT_signal
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 2 hits
TIGRFAMsiTIGR01553 formate-DH-alph, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS00932 MOLYBDOPTERIN_PROK_3, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC
60 70 80 90 100
TYCSVGCGLL MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD
110 120 130 140 150
FIHSESRLKF PEYRAPGSDK WQQISWEEAF DRIAKLMKED RDANYIAQNA
160 170 180 190 200
EGVTVNRWLS TGMLCASASS NETGYLTQKF SRALGMLAVD NQARVUHGPT
210 220 230 240 250
VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG FRWAMEAKIH
260 270 280 290 300
NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE
310 320 330 340 350
YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR
360 370 380 390 400
DTTLQHPRCV WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH
410 420 430 440 450
DKTASFLYAL GWTQHSVGAQ NIRTMAMIQL LLGNMGMAGG GVNALRGHSN
460 470 480 490 500
IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT YLTANTPKPL LEGQVNYWGN
510 520 530 540 550
YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY FEMMKEGKVN
560 570 580 590 600
GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN
610 620 630 640 650
EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG
660 670 680 690 700
EILSGIFLRL RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK
710 720 730 740 750
ALADITDPAT GAVIVKKGQQ LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ
760 770 780 790 800
MARRDNADPS GLGNTLGWAW AWPLNRRILY NRASADPQGN PWDPKRQLLK
810 820 830 840 850
WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD KMAEGPFPEH
860 870 880 890 900
YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE
910 920 930 940 950
HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA
960 970 980 990 1000
KAVVTKRIRT LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN
1010
TQTPEFKSFL VNVEKV
Length:1,016
Mass (Da):112,549
Last modified:February 26, 2008 - v5
Checksum:iDED0952028055769
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252 – 261GAKLIVIDPR → RREADCDRSC in AAB03027 (PubMed:8346018).Curated10
Sequence conflicti344 – 348ENGFA → GKRLR in AAB03027 (PubMed:8346018).Curated5

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei196Selenocysteine1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA Translation: AAB03027.2
U00096 Genomic DNA Translation: AAD13456.1
AP009048 Genomic DNA Translation: BAE77415.1
X87583 Genomic DNA Translation: CAA60887.1
PIRiA65195 S40838
RefSeqiNP_418330.1, NC_000913.3
WP_010723259.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAD13456; AAD13456; b3894
BAE77415; BAE77415; BAE77415
GeneIDi948394
KEGGiecj:JW3865
eco:b3894
PATRICifig|511145.12.peg.4008

Keywords - Coding sequence diversityi

Selenocysteine

Similar proteinsi

Entry informationi

Entry nameiFDOG_ECOLI
AccessioniPrimary (citable) accession number: P32176
Secondary accession number(s): P78131, Q2M8J1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 26, 2008
Last modified: March 28, 2018
This is version 169 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome