Rhamnulokinase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P32171 (RHAB_ECOLI)

Last modified June 16, 2009. Version 67. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Rhamnulokinase
    EC=2.7.1.5
Alternative name(s):
    Rhamnulose kinase

Gene names

Name:	rhaB
Ordered Locus Names:	b3904, JW3875

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + L-rhamnulose = ADP + L-rhamnulose 1-phosphate. HAMAP MF_01535
Pathway	Carbohydrate degradation; L-rhamnose degradation; glycerone phosphate from L-rhamnose: step 2/3. HAMAP MF_01535
Subunit structure	Monomer. Ref.7
Induction	Induced by L-rhamnose via the rhaR-rhaS regulatory cascade. Binding of the cAMP receptor protein (CRP) is required for full expression. Ref.5 Ref.6
Sequence similarities	Belongs to the rhamnulokinase family.
Sequence caution	The sequence CAA43001.1 differs from that shown. Reason: Frameshift at position 398.

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Ontologies

Keywords
Biological process	Rhamnose metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	rhamnose catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW rhamnulokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 489

489

Rhamnulokinase HAMAP MF_01535

PRO_0000090531

Regions

Region

236 – 238

Substrate binding HAMAP MF_01535

Sites

Binding site

ATP HAMAP MF_01535

Binding site

259

ATP HAMAP MF_01535

Binding site

296

Substrate HAMAP MF_01535

Binding site

304

ATP HAMAP MF_01535

Experimental info

Sequence conflict

214

Missing in CAA43001. Ref.1

Sequence conflict

388 – 389

QL → HV in CAA43001. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P32171-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: AF66259EACAC5F4E
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FASTA

489

54,069

        10         20         30         40         50         60 
MTFRNCVAVD LGASSGRVML ARYERECRSL TLREIHRFNN GLHSQNGYVT WDVDSLESAI 

        70         80         90        100        110        120 
RLGLNKVCEE GIRIDSIGID TWGVDFVLLD QQGQRVGLPV AYRDSRTNGL MAQAQQQLGK 

       130        140        150        160        170        180 
RDIYQRSGIQ FLPFNTLYQL RALTEQQPEL IPHIAHALLM PDYFSYRLTG KMNWEYTNAT 

       190        200        210        220        230        240 
TTQLVNINSD DWDESLLAWS GANKAWFGRP THPGNVIGHW ICPQGNEIPV VAVASHDTAS 

       250        260        270        280        290        300 
AVIASPLNGS RAAYLSSGTW SLMGFESQTP FTNDTALAAN ITNEGGAEGR YRVLKNIMGL 

       310        320        330        340        350        360 
WLLQRVLQEQ QINDLPALIS ATQALPACRF IINPNDDRFI NPETMCSEIQ AACRETAQPI 

       370        380        390        400        410        420 
PESDAELARC IFDSLALLYA DVLHELAQLR GEDFSQLHIV GGGCQNTLLN QLCADACGIR 

       430        440        450        460        470        480 
VIAGPVEAST LGNIGIQLMT LDELNNVDDF RQVVSTTANL TTFTPNPDSE IAHYVAQIHS 


TRQTKELCA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli." Moralejo P., Egan S.M., Hidalgo E.F., Aguilar J. J. Bacteriol. 175:5585-5594(1993) [PubMed: 8396120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A regulatory cascade in the induction of rhaBAD." Egan S.M., Schleif R.F. J. Mol. Biol. 234:87-98(1993) [PubMed: 8230210] [Abstract] Cited for: INDUCTION. Strain: ECL116.
[6]	"Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon." Holcroft C.C., Egan S.M. J. Bacteriol. 182:3529-3535(2000) [PubMed: 10852886] [Abstract] Cited for: INDUCTION.
[7]	"Structure and reaction mechanism of L-rhamnulose kinase from Escherichia coli." Grueninger D., Schulz G.E. J. Mol. Biol. 359:787-797(2006) [PubMed: 16674975] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) IN COMPLEX WITH ADP AND FRUCTOSE, SUBUNIT.

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Cross-references

Sequence databases

X60472 Genomic DNA. Translation: CAA43001.1. Frameshift.
L19201 Genomic DNA. Translation: AAB03037.1.
U00096 Genomic DNA. Translation: AAC76886.1.
AP009048 Genomic DNA. Translation: BAE77405.1.

PIR

S40848.

RefSeq

AP_003904.1.
NP_418340.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CGJ	X-ray	2.26	A	1-489	[»]
2CGK	X-ray	2.46	A/B	1-489	[»]
2CGL	X-ray	1.88	A	1-489	[»]

SMR

P32171. Positions 2-480.

ModBase

Search...

Genome annotation databases

GeneID

948399.

GenomeReviews

Gene locus JW3875 in contig AP009048_GR.
Gene locus b3904 in contig U00096_GR.

KEGG

ecj:JW3875.
eco:b3904.

Organism-specific databases

EchoBASE

EB1814.

EcoGene

EG11868. rhaB.

CMR

Search...

Phylogenomic databases

HOGENOM

P32171.

OMA

P32171. EIHRFKN.

Enzyme and pathway databases

BioCyc

EcoCyc:RHAMNULOKIN-MON.
MetaCyc:RHAMNULOKIN-MON.

Family and domain databases

HAMAP

MF_01535.
[Tree]

InterPro

IPR000577. Carb_kinase_FGGY.
IPR018484. Carb_kinase_FGGY_N.
IPR013449. Rhamnulokinase.
[Graphical view]

PANTHER

PTHR10196. FGGY_kin. 1 hit.

Pfam

PF00370. FGGY_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02627. rhamnulo_kin. 1 hit.

ProtoNet

Search...

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Entry information

Entry name

RHAB_ECOLI

Accession

Primary (citable) accession number: P32171
Secondary accession number(s): Q2M8K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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