Rhamnulokinase - Escherichia coli (strain K12)

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P32171 (RHAB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Rhamnulokinase
EC=2.7.1.5

Alternative name(s):
Rhamnulose kinase

Gene names

Name:	rhaB
Ordered Locus Names:	b3904, JW3875

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-rhamnulose = ADP + L-rhamnulose 1-phosphate. HAMAP MF_01535
Pathway	Carbohydrate degradation; L-rhamnose degradation; glycerone phosphate from L-rhamnose: step 2/3. HAMAP MF_01535
Subunit structure	Monomer. Ref.7
Induction	Induced by L-rhamnose via the rhaR-rhaS regulatory cascade. Binding of the cAMP receptor protein (CRP) is required for full expression. Ref.5 Ref.6
Sequence similarities	Belongs to the rhamnulokinase family.
Sequence caution	The sequence CAA43001.1 differs from that shown. Reason: Frameshift at position 398.

Ontologies

Keywords
Biological process	Rhamnose metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rhamnose catabolic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW rhamnulokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 489

489

Rhamnulokinase HAMAP MF_01535

PRO_0000090531

Regions

Region

236 – 238

Substrate binding HAMAP MF_01535

Sites

Binding site

ATP

Binding site

259

ATP

Binding site

296

Substrate

Binding site

304

ATP

Experimental info

Sequence conflict

214

Missing in CAA43001. Ref.1

Sequence conflict

388 – 389

QL → HV in CAA43001. Ref.1

Secondary structure

489

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32171 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: AF66259EACAC5F4E
Show »

FASTA

489

54,069

        10         20         30         40         50         60 
MTFRNCVAVD LGASSGRVML ARYERECRSL TLREIHRFNN GLHSQNGYVT WDVDSLESAI 

        70         80         90        100        110        120 
RLGLNKVCEE GIRIDSIGID TWGVDFVLLD QQGQRVGLPV AYRDSRTNGL MAQAQQQLGK 

       130        140        150        160        170        180 
RDIYQRSGIQ FLPFNTLYQL RALTEQQPEL IPHIAHALLM PDYFSYRLTG KMNWEYTNAT 

       190        200        210        220        230        240 
TTQLVNINSD DWDESLLAWS GANKAWFGRP THPGNVIGHW ICPQGNEIPV VAVASHDTAS 

       250        260        270        280        290        300 
AVIASPLNGS RAAYLSSGTW SLMGFESQTP FTNDTALAAN ITNEGGAEGR YRVLKNIMGL 

       310        320        330        340        350        360 
WLLQRVLQEQ QINDLPALIS ATQALPACRF IINPNDDRFI NPETMCSEIQ AACRETAQPI 

       370        380        390        400        410        420 
PESDAELARC IFDSLALLYA DVLHELAQLR GEDFSQLHIV GGGCQNTLLN QLCADACGIR 

       430        440        450        460        470        480 
VIAGPVEAST LGNIGIQLMT LDELNNVDDF RQVVSTTANL TTFTPNPDSE IAHYVAQIHS 


TRQTKELCA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli." Moralejo P., Egan S.M., Hidalgo E.F., Aguilar J. J. Bacteriol. 175:5585-5594(1993) [PubMed: 8396120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A regulatory cascade in the induction of rhaBAD." Egan S.M., Schleif R.F. J. Mol. Biol. 234:87-98(1993) [PubMed: 8230210] [Abstract] Cited for: INDUCTION. Strain: ECL116.
[6]	"Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon." Holcroft C.C., Egan S.M. J. Bacteriol. 182:3529-3535(2000) [PubMed: 10852886] [Abstract] Cited for: INDUCTION.
[7]	"Structure and reaction mechanism of L-rhamnulose kinase from Escherichia coli." Grueninger D., Schulz G.E. J. Mol. Biol. 359:787-797(2006) [PubMed: 16674975] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) IN COMPLEX WITH ADP AND FRUCTOSE, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X60472 Genomic DNA. Translation: CAA43001.1. Frameshift.
L19201 Genomic DNA. Translation: AAB03037.1.
U00096 Genomic DNA. Translation: AAC76886.1.
AP009048 Genomic DNA. Translation: BAE77405.1.

PIR

S40848.

RefSeq

NP_418340.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CGJ	X-ray	2.26	A	1-489	[»]
2CGK	X-ray	2.46	A/B	1-489	[»]
2CGL	X-ray	1.88	A	1-489	[»]

ProteinModelPortal

P32171.

SMR

P32171. Positions 2-480.

ModBase

Search...

Protein-protein interaction databases

IntAct

P32171. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000399; EBESCP00000000399; EBESCG00000000329.
EBESCT00000014805; EBESCP00000014096; EBESCG00000013865.

GeneID

948399.

GenomeReviews

Gene locus JW3875 in contig AP009048_GR.
Gene locus b3904 in contig U00096_GR.

KEGG

ecj:JW3875.
eco:b3904.

PATRIC

32123317. VBIEscCol129921_4019.

Organism-specific databases

EchoBASE

EB1814.

EcoGene

EG11868. rhaB.

Phylogenomic databases

eggNOG

COG1070.

GeneTree

EBGT00050000009230.

HOGENOM

HBG313195.

OMA

KENYTNE.

PhylomeDB

P32171.

ProtClustDB

PRK10640.

Enzyme and pathway databases

BioCyc

EcoCyc:RHAMNULOKIN-MONOMER.
MetaCyc:RHAMNULOKIN-MONOMER.

Gene expression databases

Genevestigator

P32171.

Family and domain databases

HAMAP

MF_01535. Rhamnulokinase.
[Tree]

InterPro

IPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018484. Carb_kinase_FGGY_N.
IPR013449. Rhamnulokinase.
[Graphical view]

K00848.

PANTHER

PTHR10196. FGGY_kin. 1 hit.

Pfam

PF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02627. Rhamnulo_kin. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RHAB_ECOLI

Accession

Primary (citable) accession number: P32171
Secondary accession number(s): Q2M8K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents