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P32169

- RHAD_ECOLI

UniProt

P32169 - RHAD_ECOLI

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Protein
Rhamnulose-1-phosphate aldolase
Gene
rhaD, rhuA, b3902, JW3873
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible cleavage of L-rhamnulose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde.UniRule annotation

Catalytic activityi

L-rhamnulose 1-phosphate = glycerone phosphate + (S)-lactaldehyde.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei117 – 1171
Metal bindingi141 – 1411Zinc
Metal bindingi143 – 1431Zinc
Metal bindingi212 – 2121Zinc

GO - Molecular functioni

  1. identical protein binding Source: EcoCyc
  2. metal ion binding Source: UniProtKB-KW
  3. rhamnulose-1-phosphate aldolase activity Source: EcoCyc

GO - Biological processi

  1. rhamnose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Rhamnose metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:RHAMNULPALDOL-MONOMER.
ECOL316407:JW3873-MONOMER.
MetaCyc:RHAMNULPALDOL-MONOMER.
UniPathwayiUPA00541; UER00603.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhamnulose-1-phosphate aldolase (EC:4.1.2.19)
Gene namesi
Name:rhaD
Synonyms:rhuA
Ordered Locus Names:b3902, JW3873
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11866. rhaD.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Rhamnulose-1-phosphate aldolaseUniRule annotation
PRO_0000209657Add
BLAST

Expressioni

Inductioni

Induced by L-rhamnose via the RhaR-RhaS regulatory cascade. Binding of the cAMP receptor protein (CRP) is required for full expression.2 Publications

Gene expression databases

GenevestigatoriP32169.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiP32169. 7 interactions.
STRINGi511145.b3902.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Helixi8 – 2215
Beta strandi31 – 366
Helixi39 – 424
Helixi43 – 486
Beta strandi54 – 574
Helixi63 – 653
Beta strandi69 – 746
Helixi79 – 813
Turni82 – 843
Helixi86 – 894
Beta strandi90 – 956
Beta strandi99 – 1079
Turni109 – 1113
Helixi113 – 1153
Helixi118 – 13114
Turni132 – 1343
Beta strandi138 – 1425
Helixi145 – 1506
Turni151 – 1533
Helixi158 – 16710
Helixi172 – 1754
Beta strandi180 – 1823
Beta strandi188 – 1903
Helixi191 – 20111
Beta strandi205 – 2095
Turni210 – 2123
Beta strandi213 – 2208
Helixi221 – 24323
Helixi256 – 2638
Helixi269 – 2724

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GT7X-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-274[»]
1OJRX-ray1.35A1-274[»]
2UYUX-ray1.96A/E1-274[»]
2UYVX-ray2.20A/B/C/D1-274[»]
2V29X-ray2.03A/B1-274[»]
2V2AX-ray1.75A1-274[»]
2V2BX-ray1.50A1-274[»]
2V9EX-ray1.58A/B1-272[»]
2V9FX-ray2.10A1-272[»]
2V9GX-ray2.70A/B/C/D1-274[»]
2V9IX-ray1.80A/B1-273[»]
2V9LX-ray1.23A1-274[»]
2V9MX-ray1.30A/B1-274[»]
2V9NX-ray1.40A/B/C/D1-274[»]
2V9OX-ray1.95A/E1-274[»]
ProteinModelPortaliP32169.
SMRiP32169. Positions 1-274.

Miscellaneous databases

EvolutionaryTraceiP32169.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0235.
HOGENOMiHOG000279628.
KOiK01629.
OMAiDMWLKGW.
OrthoDBiEOG61GGBF.
PhylomeDBiP32169.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
HAMAPiMF_00770. RhaD.
InterProiIPR001303. Aldolase_II/adducin_N.
IPR013447. Rhamnulose-1-P_Aldolase.
[Graphical view]
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
TIGRFAMsiTIGR02624. rhamnu_1P_ald. 1 hit.

Sequencei

Sequence statusi: Complete.

P32169-1 [UniParc]FASTAAdd to Basket

« Hide

MQNITQSWFV QGMIKATTDA WLKGWDERNG GNLTLRLDDA DIAPYHDNFH    50
QQPRYIPLSQ PMPLLANTPF IVTGSGKFFR NVQLDPAANL GIVKVDSDGA 100
GYHILWGLTN EAVPTSELPA HFLSHCERIK ATNGKDRVIM HCHATNLIAL 150
TYVLENDTAV FTRQLWEGST ECLVVFPDGV GILPWMVPGT DEIGQATAQE 200
MQKHSLVLWP FHGVFGSGPT LDETFGLIDT AEKSAQVLVK VYSMGGMKQT 250
ISREELIALG KRFGVTPLAS ALAL 274
Length:274
Mass (Da):30,145
Last modified:October 1, 1993 - v1
Checksum:iF9FF0BE156F4E63D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60472 Genomic DNA. Translation: CAA43003.1.
L19201 Genomic DNA. Translation: AAB03035.1.
U00096 Genomic DNA. Translation: AAC76884.1.
AP009048 Genomic DNA. Translation: BAE77407.1.
PIRiC48649.
RefSeqiNP_418338.1. NC_000913.3.
YP_491548.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76884; AAC76884; b3902.
BAE77407; BAE77407; BAE77407.
GeneIDi12931682.
948401.
KEGGiecj:Y75_p3284.
eco:b3902.
PATRICi32123313. VBIEscCol129921_4017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60472 Genomic DNA. Translation: CAA43003.1 .
L19201 Genomic DNA. Translation: AAB03035.1 .
U00096 Genomic DNA. Translation: AAC76884.1 .
AP009048 Genomic DNA. Translation: BAE77407.1 .
PIRi C48649.
RefSeqi NP_418338.1. NC_000913.3.
YP_491548.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GT7 X-ray 2.70 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-274 [» ]
1OJR X-ray 1.35 A 1-274 [» ]
2UYU X-ray 1.96 A/E 1-274 [» ]
2UYV X-ray 2.20 A/B/C/D 1-274 [» ]
2V29 X-ray 2.03 A/B 1-274 [» ]
2V2A X-ray 1.75 A 1-274 [» ]
2V2B X-ray 1.50 A 1-274 [» ]
2V9E X-ray 1.58 A/B 1-272 [» ]
2V9F X-ray 2.10 A 1-272 [» ]
2V9G X-ray 2.70 A/B/C/D 1-274 [» ]
2V9I X-ray 1.80 A/B 1-273 [» ]
2V9L X-ray 1.23 A 1-274 [» ]
2V9M X-ray 1.30 A/B 1-274 [» ]
2V9N X-ray 1.40 A/B/C/D 1-274 [» ]
2V9O X-ray 1.95 A/E 1-274 [» ]
ProteinModelPortali P32169.
SMRi P32169. Positions 1-274.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P32169. 7 interactions.
STRINGi 511145.b3902.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76884 ; AAC76884 ; b3902 .
BAE77407 ; BAE77407 ; BAE77407 .
GeneIDi 12931682.
948401.
KEGGi ecj:Y75_p3284.
eco:b3902.
PATRICi 32123313. VBIEscCol129921_4017.

Organism-specific databases

EchoBASEi EB1812.
EcoGenei EG11866. rhaD.

Phylogenomic databases

eggNOGi COG0235.
HOGENOMi HOG000279628.
KOi K01629.
OMAi DMWLKGW.
OrthoDBi EOG61GGBF.
PhylomeDBi P32169.

Enzyme and pathway databases

UniPathwayi UPA00541 ; UER00603 .
BioCyci EcoCyc:RHAMNULPALDOL-MONOMER.
ECOL316407:JW3873-MONOMER.
MetaCyc:RHAMNULPALDOL-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32169.
PROi P32169.

Gene expression databases

Genevestigatori P32169.

Family and domain databases

Gene3Di 3.40.225.10. 1 hit.
HAMAPi MF_00770. RhaD.
InterProi IPR001303. Aldolase_II/adducin_N.
IPR013447. Rhamnulose-1-P_Aldolase.
[Graphical view ]
Pfami PF00596. Aldolase_II. 1 hit.
[Graphical view ]
SMARTi SM01007. Aldolase_II. 1 hit.
[Graphical view ]
SUPFAMi SSF53639. SSF53639. 1 hit.
TIGRFAMsi TIGR02624. rhamnu_1P_ald. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli."
    Moralejo P., Egan S.M., Hidalgo E.F., Aguilar J.
    J. Bacteriol. 175:5585-5594(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A regulatory cascade in the induction of rhaBAD."
    Egan S.M., Schleif R.F.
    J. Mol. Biol. 234:87-98(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: ECL116.
  6. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  7. "Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon."
    Holcroft C.C., Egan S.M.
    J. Bacteriol. 182:3529-3535(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "The structure of L-rhamnulose-1-phosphate aldolase (class II) solved by low-resolution SIR phasing and 20-fold NCS averaging."
    Kroemer M., Schulz G.E.
    Acta Crystallogr. D 58:824-832(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
  9. "Structure and catalytic mechanism of L-rhamnulose-1-phosphate aldolase."
    Kroemer M., Merkel I., Schulz G.E.
    Biochemistry 42:10560-10568(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

Entry informationi

Entry nameiRHAD_ECOLI
AccessioniPrimary (citable) accession number: P32169
Secondary accession number(s): Q2M8J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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