Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-dihydroxy-2-naphthoate octaprenyltransferase

Gene

menA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to demethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages: the removal of the carboxyl group of DHNA as CO2, the attachment of the isoprenoid side chain, and a quinol-to-quinone oxidation, which is thought to be spontaneous.1 Publication

Catalytic activityi

An all-trans-polyprenyl diphosphate + 1,4-dihydroxy-2-naphthoate = a demethylmenaquinol + diphosphate + CO2.UniRule annotation1 Publication

Pathwayi: menaquinone biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
  2. Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)
This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

  • 1,4-dihydroxy-2-naphthoate octaprenyltransferase activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

GO - Biological processi

  • menaquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:DMK-MONOMER.
ECOL316407:JW3901-MONOMER.
MetaCyc:DMK-MONOMER.
BRENDAi2.5.1.74. 2026.
UniPathwayiUPA00079; UER00168.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-dihydroxy-2-naphthoate octaprenyltransferaseUniRule annotation (EC:2.5.1.74UniRule annotation1 Publication)
Short name:
DHNA-octaprenyltransferaseUniRule annotation
Gene namesi
Name:menA1 PublicationUniRule annotation
Synonyms:yiiW
Ordered Locus Names:b3930, JW3901
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11880. menA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicCuratedAdd
BLAST
Transmembranei21 – 4121HelicalUniRule annotationAdd
BLAST
Topological domaini42 – 421PeriplasmicCurated
Transmembranei43 – 6321HelicalUniRule annotationAdd
BLAST
Topological domaini64 – 9734CytoplasmicCuratedAdd
BLAST
Transmembranei98 – 11821HelicalUniRule annotationAdd
BLAST
Topological domaini119 – 1235PeriplasmicCurated
Transmembranei124 – 14421HelicalUniRule annotationAdd
BLAST
Topological domaini145 – 1484CytoplasmicCurated
Transmembranei149 – 16921HelicalUniRule annotationAdd
BLAST
Topological domaini170 – 1767PeriplasmicCurated
Transmembranei177 – 19721HelicalUniRule annotationAdd
BLAST
Topological domaini198 – 22730CytoplasmicCuratedAdd
BLAST
Transmembranei228 – 24720HelicalUniRule annotationAdd
BLAST
Topological domaini248 – 2503PeriplasmicCurated
Transmembranei251 – 27020HelicalUniRule annotationAdd
BLAST
Topological domaini271 – 28616CytoplasmicCuratedAdd
BLAST
Transmembranei287 – 30721HelicalUniRule annotationAdd
BLAST
Topological domaini308 – 3081Periplasmic1 Publication

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3083081,4-dihydroxy-2-naphthoate octaprenyltransferasePRO_0000096414Add
BLAST

Proteomic databases

PaxDbiP32166.

Interactioni

Protein-protein interaction databases

BioGridi4261778. 7 interactions.
STRINGi511145.b3930.

Structurei

3D structure databases

ProteinModelPortaliP32166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MenA family. Type 1 subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EIS. Bacteria.
COG1575. LUCA.
HOGENOMiHOG000028297.
InParanoidiP32166.
KOiK02548.
OMAiMGLFYVF.
PhylomeDBiP32166.

Family and domain databases

HAMAPiMF_01937. MenA_1. 1 hit.
InterProiIPR004657. MenA.
IPR000537. UbiA_prenyltransferase.
IPR026046. UBIAD1.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
PIRSFiPIRSF005355. UBIAD1. 1 hit.

Sequencei

Sequence statusi: Complete.

P32166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQQISRTQ AWLESLRPKT LPLAFAAIIV GTALAWWQGH FDPLVALLAL
60 70 80 90 100
ITAGLLQILS NLANDYGDAV KGSDKPDRIG PLRGMQKGVI TQQEMKRALI
110 120 130 140 150
ITVVLICLSG LALVAVACHT LADFVGFLIL GGLSIIAAIT YTVGNRPYGY
160 170 180 190 200
IGLGDISVLV FFGWLSVMGS WYLQAHTLIP ALILPATACG LLATAVLNIN
210 220 230 240 250
NLRDINSDRE NGKNTLVVRL GEVNARRYHA CLLMGSLVCL ALFNLFSLHS
260 270 280 290 300
LWGWLFLLAA PLLVKQARYV MREMDPVAMR PMLERTVKGA LLTNLLFVLG

IFLSQWAA
Length:308
Mass (Da):33,594
Last modified:October 1, 1993 - v1
Checksum:iFECD9E85BAA40E2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56082 Genomic DNA. Translation: AAB01207.1.
L19201 Genomic DNA. Translation: AAB03062.1.
U00096 Genomic DNA. Translation: AAC76912.1.
AP009048 Genomic DNA. Translation: BAE77380.1.
PIRiS40873.
RefSeqiNP_418365.1. NC_000913.3.
WP_000139496.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76912; AAC76912; b3930.
BAE77380; BAE77380; BAE77380.
GeneIDi948418.
KEGGiecj:JW3901.
eco:b3930.
PATRICi32123375. VBIEscCol129921_4048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56082 Genomic DNA. Translation: AAB01207.1.
L19201 Genomic DNA. Translation: AAB03062.1.
U00096 Genomic DNA. Translation: AAC76912.1.
AP009048 Genomic DNA. Translation: BAE77380.1.
PIRiS40873.
RefSeqiNP_418365.1. NC_000913.3.
WP_000139496.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261778. 7 interactions.
STRINGi511145.b3930.

Proteomic databases

PaxDbiP32166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76912; AAC76912; b3930.
BAE77380; BAE77380; BAE77380.
GeneIDi948418.
KEGGiecj:JW3901.
eco:b3930.
PATRICi32123375. VBIEscCol129921_4048.

Organism-specific databases

EchoBASEiEB1826.
EcoGeneiEG11880. menA.

Phylogenomic databases

eggNOGiENOG4105EIS. Bacteria.
COG1575. LUCA.
HOGENOMiHOG000028297.
InParanoidiP32166.
KOiK02548.
OMAiMGLFYVF.
PhylomeDBiP32166.

Enzyme and pathway databases

UniPathwayiUPA00079; UER00168.
BioCyciEcoCyc:DMK-MONOMER.
ECOL316407:JW3901-MONOMER.
MetaCyc:DMK-MONOMER.
BRENDAi2.5.1.74. 2026.

Miscellaneous databases

PROiP32166.

Family and domain databases

HAMAPiMF_01937. MenA_1. 1 hit.
InterProiIPR004657. MenA.
IPR000537. UbiA_prenyltransferase.
IPR026046. UBIAD1.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
PIRSFiPIRSF005355. UBIAD1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMENA_ECOLI
AccessioniPrimary (citable) accession number: P32166
Secondary accession number(s): Q2M8M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.