Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32156

- RHAM_ECOLI

UniProt

P32156 - RHAM_ECOLI

Protein

L-rhamnose mutarotase

Gene

rhaM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in the anomeric conversion of L-rhamnose.1 Publication

    Catalytic activityi

    Alpha-L-rhamnose = beta-L-rhamnose.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei18 – 181Substrate
    Active sitei22 – 221Proton donorCurated
    Binding sitei41 – 411Substrate

    GO - Molecular functioni

    1. racemase and epimerase activity, acting on carbohydrates and derivatives Source: EcoCyc

    GO - Biological processi

    1. rhamnose catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism, Rhamnose metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11865-MONOMER.
    ECOL316407:JW3872-MONOMER.
    MetaCyc:EG11865-MONOMER.
    UniPathwayiUPA00125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-rhamnose mutarotase (EC:5.1.3.n3)
    Alternative name(s):
    Rhamnose 1-epimerase
    Type-3 mutarotase
    Gene namesi
    Name:rhaM
    Synonyms:yiiL
    Ordered Locus Names:b3901, JW3872
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11865. rhaM.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi18 – 181Y → F: Loss of mutarotase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 104104L-rhamnose mutarotasePRO_0000169687Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP32156.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gloAP0AC811EBI-1134000,EBI-551143

    Protein-protein interaction databases

    IntActiP32156. 2 interactions.
    STRINGi511145.b3901.

    Structurei

    Secondary structure

    1
    104
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Helixi15 – 206
    Turni21 – 244
    Helixi27 – 359
    Beta strandi38 – 469
    Turni47 – 504
    Beta strandi51 – 599
    Helixi61 – 666
    Helixi67 – 693
    Helixi71 – 8010
    Turni81 – 833
    Beta strandi94 – 963
    Beta strandi98 – 1036

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1X8DX-ray1.80A/B/C/D1-104[»]
    ProteinModelPortaliP32156.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32156.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 772Substrate binding

    Sequence similaritiesi

    Belongs to the rhamnose mutarotase family.Curated

    Phylogenomic databases

    eggNOGiCOG3254.
    HOGENOMiHOG000250144.
    KOiK03534.
    OMAiESEARWN.
    OrthoDBiEOG64NB3R.
    PhylomeDBiP32156.

    Family and domain databases

    HAMAPiMF_01663. L_rham_rotase.
    InterProiIPR011008. Dimeric_a/b-barrel.
    IPR013448. L-rhamnose_1-epimerase.
    IPR008000. Rhamnose_mutarotase.
    [Graphical view]
    PfamiPF05336. DUF718. 1 hit.
    [Graphical view]
    SUPFAMiSSF54909. SSF54909. 1 hit.
    TIGRFAMsiTIGR02625. YiiL_rotase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32156-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRKAFVMQV NPDAHEEYQR RHNPIWPELE AVLKSHGAHN YAIYLDKARN    50
    LLFAMVEIES EERWNAVAST DVCQRWWKYM TDVMPANPDN SPVSSELQEV 100
    FYLP 104
    Length:104
    Mass (Da):12,265
    Last modified:October 1, 1993 - v1
    Checksum:i6A276C08E0EEC7E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19201 Genomic DNA. Translation: AAB03034.1.
    U00096 Genomic DNA. Translation: AAC76883.1.
    AP009048 Genomic DNA. Translation: BAE77408.1.
    PIRiS40845.
    RefSeqiNP_418337.1. NC_000913.3.
    YP_491549.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76883; AAC76883; b3901.
    BAE77408; BAE77408; BAE77408.
    GeneIDi12933648.
    948402.
    KEGGiecj:Y75_p3285.
    eco:b3901.
    PATRICi32123311. VBIEscCol129921_4016.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19201 Genomic DNA. Translation: AAB03034.1 .
    U00096 Genomic DNA. Translation: AAC76883.1 .
    AP009048 Genomic DNA. Translation: BAE77408.1 .
    PIRi S40845.
    RefSeqi NP_418337.1. NC_000913.3.
    YP_491549.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1X8D X-ray 1.80 A/B/C/D 1-104 [» ]
    ProteinModelPortali P32156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P32156. 2 interactions.
    STRINGi 511145.b3901.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76883 ; AAC76883 ; b3901 .
    BAE77408 ; BAE77408 ; BAE77408 .
    GeneIDi 12933648.
    948402.
    KEGGi ecj:Y75_p3285.
    eco:b3901.
    PATRICi 32123311. VBIEscCol129921_4016.

    Organism-specific databases

    EchoBASEi EB1811.
    EcoGenei EG11865. rhaM.

    Phylogenomic databases

    eggNOGi COG3254.
    HOGENOMi HOG000250144.
    KOi K03534.
    OMAi ESEARWN.
    OrthoDBi EOG64NB3R.
    PhylomeDBi P32156.

    Enzyme and pathway databases

    UniPathwayi UPA00125 .
    BioCyci EcoCyc:EG11865-MONOMER.
    ECOL316407:JW3872-MONOMER.
    MetaCyc:EG11865-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32156.
    PROi P32156.

    Gene expression databases

    Genevestigatori P32156.

    Family and domain databases

    HAMAPi MF_01663. L_rham_rotase.
    InterProi IPR011008. Dimeric_a/b-barrel.
    IPR013448. L-rhamnose_1-epimerase.
    IPR008000. Rhamnose_mutarotase.
    [Graphical view ]
    Pfami PF05336. DUF718. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    TIGRFAMsi TIGR02625. YiiL_rotase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
      Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "NMR application probes a novel and ubiquitous family of enzymes that alter monosaccharide configuration."
      Ryu K.-S., Kim C., Kim I., Yoo S., Choi B.-S., Park C.
      J. Biol. Chem. 279:25544-25548(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A MUTAROTASE.
    5. "Structural insights into the monosaccharide specificity of Escherichia coli rhamnose mutarotase."
      Ryu K.-S., Kim J.-I., Cho S.-J., Park D., Park C., Cheong H.-K., Lee J.-O., Choi B.-S.
      J. Mol. Biol. 349:153-162(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH L-RHAMNOSE, SUBUNIT, MUTAGENESIS OF TYR-18, REACTION MECHANISM.

    Entry informationi

    Entry nameiRHAM_ECOLI
    AccessioniPrimary (citable) accession number: P32156
    Secondary accession number(s): Q2M8J8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3