Reviewed, UniProtKB/Swiss-Prot P32154 (PTFLB_ECOLI)

Names and origin

Protein names

Recommended name:
    Fructose-like PTS system EIIBC component
Including the following 2 domains:
    1- Recommended name:
            Fructose-like phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system fructose-like EIIB component
    2- Recommended name:
            Fructose-like permease IIC component
        Alternative name(s):
            PTS system fructose-like EIIC component

Gene names

Name:	frvB
Synonyms:	yiiJ
Ordered Locus Names:	b3899, JW5562

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.5
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Fructose-like PTS system EIIBC component		PRO_0000186499
Regions
	Transmembrane	132 – 152	21	Potential
	Transmembrane	180 – 200	21	Potential
	Transmembrane	204 – 224	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	264 – 284	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	344 – 364	21	Potential
	Transmembrane	380 – 400	21	Potential
	Transmembrane	402 – 422	21	Potential
	Transmembrane	442 – 462	21	Potential
	Domain	1 – 105	105	PTS EIIB type-2
	Domain	128 – 475	348	PTS EIIC type-2
Sites
	Active site	13	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32154-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 9B982ECDCBA1E1A4	FASTA	483	51,120
	10 20 30 40 50 60 MESSLRIVAI TNCPAGIAHT YMVAEALEQK ARSLGHTIKV ETQGSSGVEN RLSSEEIAAA 70 80 90 100 110 120 DYVILATGRG LSGDDRARFA GKKVYEIAIS QALKNIDQIF SELPTNSQLF AADSGVKLGK 130 140 150 160 170 180 QEVQSGSVMS HLMAGVSAAL PFVIGGGILV ALANMLVQFG LPYTDMSKGA PSFTWVVESI 190 200 210 220 230 240 GYLGFTFMIP IMGAYIASSI ADKPAFAPAF LVCYLANDKA LLGTQSGAGF LGAVVLGLAI 250 260 270 280 290 300 GYFVFWFRKV RLGKALQPLL GSMLIPFVTL LVFGVLTYYV IGPVMSDLMG GLLHFLNTIP 310 320 330 340 350 360 PSMKFAAAFL VGAMLAFDMG GPINKTAWFF CFSLLEKHIY DWYAIVGVVA LMPPVAAGLA 370 380 390 400 410 420 TFIAPKLFTR QEKEAASSAI VVGATVATEP AIPYALAAPL PMITANTLAG GITGVLVIAF 430 440 450 460 470 480 GIKRLAPGLG IFDPLIGLMS PVGSFYLVLA IGLALNISFI IVLKGLWLRR KAKAAQQELV HEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems." Reizer J., Michotey V., Reizer A., Saier M.H. Jr. Protein Sci. 3:440-450(1994) [PubMed: 8019415] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19201 Genomic DNA. Translation: AAB03032.1. Different initiation. U00096 Genomic DNA. Translation: AAC76881.1. Different initiation. AP009048 Genomic DNA. Translation: BAE77410.1.
RefSeq	AP_003909.1. NP_418335.4.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P32154.
Genome annotation databases
GeneID	948390.
GenomeReviews	Gene locus JW5562 in contig AP009048_GR. Gene locus b3899 in contig U00096_GR.
KEGG	ecj:JW5562. eco:b3899.
Organism-specific databases
EchoBASE	EB1809.
EcoGene	EG11863. frvB.
CMR	Search...
Phylogenomic databases
HOGENOM	P32154.
OMA	YEVHVET.
Enzyme and pathway databases
BioCyc	EcoCyc:FRVB-MON.
Gene expression databases
Genevestigator	P32154.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003353. PTS_IIB_fruc. IPR006327. PTS_IIC_fruc. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02379. PTS_IIB_fruc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00829. FRU. 1 hit. TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFLB_ECOLI

Accession

Primary (citable) accession number: P32154
Secondary accession number(s): Q2M8J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	April 27, 2001
Last modified:	November 3, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)