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Protein

Fructose-like PTS system EIIBC component

Gene

frvB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FrvAB PTS system is involved in fructose transport.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13Phosphocysteine intermediate; for EIIB activityBy similarityCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:FRVB-MONOMER.
ECOL316407:JW5562-MONOMER.
MetaCyc:FRVB-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.25. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-like PTS system EIIBC component1 Publication
Including the following 2 domains:
PTS system fructose-like EIIB component1 Publication (EC:2.7.1.202By similarity)
Alternative name(s):
Fructose-like phosphotransferase enzyme IIB component1 Publication
PTS system fructose-like EIIC component1 Publication
Alternative name(s):
Fructose-like permease IIC component1 Publication
Gene namesi
Name:frvB1 Publication
Synonyms:yiiJ
Ordered Locus Names:b3899, JW5562
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11863. frvB.

Subcellular locationi

  • Cell inner membrane PROSITE-ProRule annotation1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei132 – 152HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei180 – 200HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei204 – 224HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei227 – 247HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei264 – 284HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei303 – 323HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei344 – 364HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei380 – 400HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei402 – 422HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei442 – 462HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864991 – 483Fructose-like PTS system EIIBC componentAdd BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP32154.
PRIDEiP32154.

Interactioni

Protein-protein interaction databases

BioGridi4259396. 16 interactors.
STRINGi511145.b3899.

Structurei

3D structure databases

ProteinModelPortaliP32154.
SMRiP32154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 105PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST105
Domaini128 – 475PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST348

Domaini

The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
HOGENOMiHOG000227677.
KOiK11202.
K11203.
OMAiISTWIRP.
PhylomeDBiP32154.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSLRIVAI TNCPAGIAHT YMVAEALEQK ARSLGHTIKV ETQGSSGVEN
60 70 80 90 100
RLSSEEIAAA DYVILATGRG LSGDDRARFA GKKVYEIAIS QALKNIDQIF
110 120 130 140 150
SELPTNSQLF AADSGVKLGK QEVQSGSVMS HLMAGVSAAL PFVIGGGILV
160 170 180 190 200
ALANMLVQFG LPYTDMSKGA PSFTWVVESI GYLGFTFMIP IMGAYIASSI
210 220 230 240 250
ADKPAFAPAF LVCYLANDKA LLGTQSGAGF LGAVVLGLAI GYFVFWFRKV
260 270 280 290 300
RLGKALQPLL GSMLIPFVTL LVFGVLTYYV IGPVMSDLMG GLLHFLNTIP
310 320 330 340 350
PSMKFAAAFL VGAMLAFDMG GPINKTAWFF CFSLLEKHIY DWYAIVGVVA
360 370 380 390 400
LMPPVAAGLA TFIAPKLFTR QEKEAASSAI VVGATVATEP AIPYALAAPL
410 420 430 440 450
PMITANTLAG GITGVLVIAF GIKRLAPGLG IFDPLIGLMS PVGSFYLVLA
460 470 480
IGLALNISFI IVLKGLWLRR KAKAAQQELV HEH
Length:483
Mass (Da):51,120
Last modified:April 27, 2001 - v2
Checksum:i9B982ECDCBA1E1A4
GO

Sequence cautioni

The sequence AAB03032 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03032.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76881.2.
AP009048 Genomic DNA. Translation: BAE77410.1.
RefSeqiNP_418335.4. NC_000913.3.
WP_000446023.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76881; AAC76881; b3899.
BAE77410; BAE77410; BAE77410.
GeneIDi948390.
KEGGiecj:JW5562.
eco:b3899.
PATRICi32123307. VBIEscCol129921_4014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03032.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76881.2.
AP009048 Genomic DNA. Translation: BAE77410.1.
RefSeqiNP_418335.4. NC_000913.3.
WP_000446023.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32154.
SMRiP32154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259396. 16 interactors.
STRINGi511145.b3899.

Protein family/group databases

TCDBi4.A.2.1.25. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP32154.
PRIDEiP32154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76881; AAC76881; b3899.
BAE77410; BAE77410; BAE77410.
GeneIDi948390.
KEGGiecj:JW5562.
eco:b3899.
PATRICi32123307. VBIEscCol129921_4014.

Organism-specific databases

EchoBASEiEB1809.
EcoGeneiEG11863. frvB.

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
HOGENOMiHOG000227677.
KOiK11202.
K11203.
OMAiISTWIRP.
PhylomeDBiP32154.

Enzyme and pathway databases

BioCyciEcoCyc:FRVB-MONOMER.
ECOL316407:JW5562-MONOMER.
MetaCyc:FRVB-MONOMER.

Miscellaneous databases

PROiP32154.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTFLB_ECOLI
AccessioniPrimary (citable) accession number: P32154
Secondary accession number(s): Q2M8J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.