Fructose-like PTS system EIIBC component - Escherichia coli (strain K12)

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P32154 (PTFLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Fructose-like PTS system EIIBC component

Including the following 2 domains:

Fructose-like phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system fructose-like EIIB component
Fructose-like permease IIC component
Alternative name(s):
PTS system fructose-like EIIC component

Gene names

Name:	frvB
Synonyms:	yiiJ
Ordered Locus Names:	b3899, JW5562

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.
Sequence caution	The sequence AAB03032.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 483

483

Fructose-like PTS system EIIBC component

PRO_0000186499

Regions

Transmembrane

132 – 152

Helical; Potential

Transmembrane

180 – 200

Helical; Potential

Transmembrane

204 – 224

Helical; Potential

Transmembrane

227 – 247

Helical; Potential

Transmembrane

264 – 284

Helical; Potential

Transmembrane

303 – 323

Helical; Potential

Transmembrane

344 – 364

Helical; Potential

Transmembrane

380 – 400

Helical; Potential

Transmembrane

402 – 422

Helical; Potential

Transmembrane

442 – 462

Helical; Potential

Domain

1 – 105

105

PTS EIIB type-2

Domain

128 – 475

348

PTS EIIC type-2

Sites

Active site

Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P32154 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 9B982ECDCBA1E1A4
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FASTA

483

51,120

        10         20         30         40         50         60 
MESSLRIVAI TNCPAGIAHT YMVAEALEQK ARSLGHTIKV ETQGSSGVEN RLSSEEIAAA 

        70         80         90        100        110        120 
DYVILATGRG LSGDDRARFA GKKVYEIAIS QALKNIDQIF SELPTNSQLF AADSGVKLGK 

       130        140        150        160        170        180 
QEVQSGSVMS HLMAGVSAAL PFVIGGGILV ALANMLVQFG LPYTDMSKGA PSFTWVVESI 

       190        200        210        220        230        240 
GYLGFTFMIP IMGAYIASSI ADKPAFAPAF LVCYLANDKA LLGTQSGAGF LGAVVLGLAI 

       250        260        270        280        290        300 
GYFVFWFRKV RLGKALQPLL GSMLIPFVTL LVFGVLTYYV IGPVMSDLMG GLLHFLNTIP 

       310        320        330        340        350        360 
PSMKFAAAFL VGAMLAFDMG GPINKTAWFF CFSLLEKHIY DWYAIVGVVA LMPPVAAGLA 

       370        380        390        400        410        420 
TFIAPKLFTR QEKEAASSAI VVGATVATEP AIPYALAAPL PMITANTLAG GITGVLVIAF 

       430        440        450        460        470        480 
GIKRLAPGLG IFDPLIGLMS PVGSFYLVLA IGLALNISFI IVLKGLWLRR KAKAAQQELV 


HEH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems." Reizer J., Michotey V., Reizer A., Saier M.H. Jr. Protein Sci. 3:440-450(1994) [PubMed: 8019415] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19201 Genomic DNA. Translation: AAB03032.1. Different initiation. U00096 Genomic DNA. Translation: AAC76881.2. AP009048 Genomic DNA. Translation: BAE77410.1.
RefSeq	NP_418335.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P32154.
SMR	P32154. Positions 4-109.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002508; EBESCP00000002508; EBESCG00000002047. EBESCT00000015727; EBESCP00000015018; EBESCG00000014787.
GeneID	948390.
GenomeReviews	Gene locus JW5562 in contig AP009048_GR. Gene locus b3899 in contig U00096_GR.
KEGG	ecj:JW5562. eco:b3899.
PATRIC	32123307. VBIEscCol129921_4014.
Organism-specific databases
EchoBASE	EB1809.
EcoGene	EG11863. frvB.
Phylogenomic databases
eggNOG	COG1299.
GeneTree	EBGT00070000031702.
HOGENOM	HBG704192.
OMA	YEVHVET.
PhylomeDB	P32154.
ProtClustDB	PRK11404.
Enzyme and pathway databases
BioCyc	EcoCyc:FRVB-MONOMER.
Gene expression databases
Genevestigator	P32154.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003353. PTS_IIB_fruc. IPR006327. PTS_IIC_fruc. [Graphical view]
KO	K11202. K11203.
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00829. FRU. 1 hit. TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFLB_ECOLI

Accession

Primary (citable) accession number: P32154
Secondary accession number(s): Q2M8J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents