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Protein

Fructose-like PTS system EIIBC component

Gene

frvB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131Phosphocysteine intermediate; for EIIB activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:FRVB-MONOMER.
ECOL316407:JW5562-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.25. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-like PTS system EIIBC component
Including the following 2 domains:
Fructose-like phosphotransferase enzyme IIB component (EC:2.7.1.202)
Alternative name(s):
PTS system fructose-like EIIB component
Fructose-like permease IIC component
Alternative name(s):
PTS system fructose-like EIIC component
Gene namesi
Name:frvB
Synonyms:yiiJ
Ordered Locus Names:b3899, JW5562
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11863. frvB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei132 – 15221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei180 – 20021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei204 – 22421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei227 – 24721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei264 – 28421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei303 – 32321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei344 – 36421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei380 – 40021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei402 – 42221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei442 – 46221HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Fructose-like PTS system EIIBC componentPRO_0000186499Add
BLAST

Proteomic databases

PaxDbiP32154.

Interactioni

Protein-protein interaction databases

BioGridi4259396. 16 interactions.
STRINGi511145.b3899.

Structurei

3D structure databases

ProteinModelPortaliP32154.
SMRiP32154. Positions 7-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 105105PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST
Domaini128 – 475348PTS EIIC type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
HOGENOMiHOG000227677.
KOiK11202.
K11203.
OMAiISTWIRP.
PhylomeDBiP32154.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSLRIVAI TNCPAGIAHT YMVAEALEQK ARSLGHTIKV ETQGSSGVEN
60 70 80 90 100
RLSSEEIAAA DYVILATGRG LSGDDRARFA GKKVYEIAIS QALKNIDQIF
110 120 130 140 150
SELPTNSQLF AADSGVKLGK QEVQSGSVMS HLMAGVSAAL PFVIGGGILV
160 170 180 190 200
ALANMLVQFG LPYTDMSKGA PSFTWVVESI GYLGFTFMIP IMGAYIASSI
210 220 230 240 250
ADKPAFAPAF LVCYLANDKA LLGTQSGAGF LGAVVLGLAI GYFVFWFRKV
260 270 280 290 300
RLGKALQPLL GSMLIPFVTL LVFGVLTYYV IGPVMSDLMG GLLHFLNTIP
310 320 330 340 350
PSMKFAAAFL VGAMLAFDMG GPINKTAWFF CFSLLEKHIY DWYAIVGVVA
360 370 380 390 400
LMPPVAAGLA TFIAPKLFTR QEKEAASSAI VVGATVATEP AIPYALAAPL
410 420 430 440 450
PMITANTLAG GITGVLVIAF GIKRLAPGLG IFDPLIGLMS PVGSFYLVLA
460 470 480
IGLALNISFI IVLKGLWLRR KAKAAQQELV HEH
Length:483
Mass (Da):51,120
Last modified:April 27, 2001 - v2
Checksum:i9B982ECDCBA1E1A4
GO

Sequence cautioni

The sequence AAB03032 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03032.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76881.2.
AP009048 Genomic DNA. Translation: BAE77410.1.
RefSeqiNP_418335.4. NC_000913.3.
WP_000446023.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76881; AAC76881; b3899.
BAE77410; BAE77410; BAE77410.
GeneIDi948390.
KEGGiecj:JW5562.
eco:b3899.
PATRICi32123307. VBIEscCol129921_4014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03032.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76881.2.
AP009048 Genomic DNA. Translation: BAE77410.1.
RefSeqiNP_418335.4. NC_000913.3.
WP_000446023.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32154.
SMRiP32154. Positions 7-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259396. 16 interactions.
STRINGi511145.b3899.

Protein family/group databases

TCDBi4.A.2.1.25. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP32154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76881; AAC76881; b3899.
BAE77410; BAE77410; BAE77410.
GeneIDi948390.
KEGGiecj:JW5562.
eco:b3899.
PATRICi32123307. VBIEscCol129921_4014.

Organism-specific databases

EchoBASEiEB1809.
EcoGeneiEG11863. frvB.

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
HOGENOMiHOG000227677.
KOiK11202.
K11203.
OMAiISTWIRP.
PhylomeDBiP32154.

Enzyme and pathway databases

BioCyciEcoCyc:FRVB-MONOMER.
ECOL316407:JW5562-MONOMER.

Miscellaneous databases

PROiP32154.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTFLB_ECOLI
AccessioniPrimary (citable) accession number: P32154
Secondary accession number(s): Q2M8J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.