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P32138

- YIHQ_ECOLI

UniProt

P32138 - YIHQ_ECOLI

Protein

Alpha-glucosidase YihQ

Gene

yihQ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Exhibits hydrolysis activity against alpha-glucosyl fluoride, although natural substrates, such as alpha-glucobioses are scarcely hydrolyzed.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei405 – 4051NucleophileBy similarity
    Active sitei408 – 4081By similarity
    Active sitei472 – 4721Proton donorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. maltose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11843-MONOMER.
    ECOL316407:JW3849-MONOMER.

    Protein family/group databases

    CAZyiGH31. Glycoside Hydrolase Family 31.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-glucosidase YihQ (EC:3.2.1.20)
    Gene namesi
    Name:yihQ
    Ordered Locus Names:b3878, JW3849
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11843. yihQ.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 678678Alpha-glucosidase YihQPRO_0000185372Add
    BLAST

    Expressioni

    Inductioni

    Induced during growth with sulfoquinovose.1 Publication

    Gene expression databases

    GenevestigatoriP32138.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-12498N.
    IntActiP32138. 1 interaction.
    MINTiMINT-1249337.
    STRINGi511145.b3878.

    Structurei

    3D structure databases

    ProteinModelPortaliP32138.
    SMRiP32138. Positions 309-635.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.Curated

    Phylogenomic databases

    eggNOGiCOG1501.
    HOGENOMiHOG000064244.
    KOiK15922.
    OMAiKYQYLFG.
    OrthoDBiEOG6Z99WN.
    PhylomeDBiP32138.

    Family and domain databases

    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01055. Glyco_hydro_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32138-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTPRPQLLD FQFHQNNDSF TLHFQQRLIL THSKDNPCLW IGSGIADIDM    50
    FRGNFSIKDK LQEKIALTDA IVSQSPDGWL IHFSRGSDIS ATLNISADDQ 100
    GRLLLELQND NLNHNRIWLR LAAQPEDHIY GCGEQFSYFD LRGKPFPLWT 150
    SEQGVGRNKQ TYVTWQADCK ENAGGDYYWT FFPQPTFVST QKYYCHVDNS 200
    CYMNFDFSAP EYHELALWED KATLRFECAD TYISLLEKLT ALLGRQPELP 250
    DWIYDGVTLG IQGGTEVCQK KLDTMRNAGV KVNGIWAQDW SGIRMTSFGK 300
    RVMWNWKWNS ENYPQLDSRI KQWNQEGVQF LAYINPYVAS DKDLCEEAAQ 350
    HGYLAKDASG GDYLVEFGEF YGGVVDLTNP EAYAWFKEVI KKNMIELGCG 400
    GWMADFGEYL PTDTYLHNGV SAEIMHNAWP ALWAKCNYEA LEETGKLGEI 450
    LFFMRAGSTG SQKYSTMMWA GDQNVDWSLD DGLASVVPAA LSLAMTGHGL 500
    HHSDIGGYTT LFEMKRSKEL LLRWCDFSAF TPMMRTHEGN RPGDNWQFDG 550
    DAETIAHFAR MTTVFTTLKP YLKEAVALNA KSGLPVMRPL FLHYEDDAHT 600
    YTLKYQYLLG RDILVAPVHE EGRSDWTLYL PEDNWVHAWT GEAFRGGEVT 650
    VNAPIGKPPV FYRADSEWAA LFASLKSI 678
    Length:678
    Mass (Da):77,275
    Last modified:July 15, 1998 - v3
    Checksum:i85869F52BB72FEE7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti358 – 3581A → R in AAB03011. (PubMed:8346018)Curated
    Sequence conflicti517 – 5171S → T in AAB03011. (PubMed:8346018)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19201 Genomic DNA. Translation: AAB03011.1.
    U00096 Genomic DNA. Translation: AAC76875.1.
    AP009048 Genomic DNA. Translation: BAE77431.1.
    PIRiA65193.
    RefSeqiNP_418314.1. NC_000913.3.
    YP_491572.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76875; AAC76875; b3878.
    BAE77431; BAE77431; BAE77431.
    GeneIDi12931872.
    948376.
    KEGGiecj:Y75_p3308.
    eco:b3878.
    PATRICi32123259. VBIEscCol129921_3990.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19201 Genomic DNA. Translation: AAB03011.1 .
    U00096 Genomic DNA. Translation: AAC76875.1 .
    AP009048 Genomic DNA. Translation: BAE77431.1 .
    PIRi A65193.
    RefSeqi NP_418314.1. NC_000913.3.
    YP_491572.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P32138.
    SMRi P32138. Positions 309-635.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-12498N.
    IntActi P32138. 1 interaction.
    MINTi MINT-1249337.
    STRINGi 511145.b3878.

    Protein family/group databases

    CAZyi GH31. Glycoside Hydrolase Family 31.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76875 ; AAC76875 ; b3878 .
    BAE77431 ; BAE77431 ; BAE77431 .
    GeneIDi 12931872.
    948376.
    KEGGi ecj:Y75_p3308.
    eco:b3878.
    PATRICi 32123259. VBIEscCol129921_3990.

    Organism-specific databases

    EchoBASEi EB1789.
    EcoGenei EG11843. yihQ.

    Phylogenomic databases

    eggNOGi COG1501.
    HOGENOMi HOG000064244.
    KOi K15922.
    OMAi KYQYLFG.
    OrthoDBi EOG6Z99WN.
    PhylomeDBi P32138.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11843-MONOMER.
    ECOL316407:JW3849-MONOMER.

    Miscellaneous databases

    PROi P32138.

    Gene expression databases

    Genevestigatori P32138.

    Family and domain databases

    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
      Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 358 AND 517.
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli."
      Okuyama M., Mori H., Chiba S., Kimura A.
      Protein Expr. Purif. 37:170-179(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Sulphoglycolysis in Escherichia coli K-12 closes a gap in the biogeochemical sulphur cycle."
      Denger K., Weiss M., Felux A.K., Schneider A., Mayer C., Spiteller D., Huhn T., Cook A.M., Schleheck D.
      Nature 507:114-117(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
      Strain: K12.

    Entry informationi

    Entry nameiYIHQ_ECOLI
    AccessioniPrimary (citable) accession number: P32138
    Secondary accession number(s): P76775, Q2M8H5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3