P32138 (YIHQ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-glucosidase YihQ
EC=3.2.1.20

Gene names

Name:	yihQ
Ordered Locus Names:	b3878, JW3849

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exhibits hydrolysis activity against alpha-glucosyl fluoride, although natural substrates, such as alpha-glucobioses are scarcely hydrolyzed. Ref.4
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Alpha-glucosidase YihQ		PRO_0000185372
Sites
	Active site	405	1	Nucleophile By similarity
	Active site	408	1	By similarity
	Active site	472	1	Proton donor By similarity
Experimental info
	Sequence conflict	358	1	A → R in AAB03011. Ref.1
	Sequence conflict	517	1	S → T in AAB03011. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32138 [UniParc]. Last modified July 15, 1998. Version 3. Checksum: 85869F52BB72FEE7	FASTA	678	77,275
	10 20 30 40 50 60 MDTPRPQLLD FQFHQNNDSF TLHFQQRLIL THSKDNPCLW IGSGIADIDM FRGNFSIKDK 70 80 90 100 110 120 LQEKIALTDA IVSQSPDGWL IHFSRGSDIS ATLNISADDQ GRLLLELQND NLNHNRIWLR 130 140 150 160 170 180 LAAQPEDHIY GCGEQFSYFD LRGKPFPLWT SEQGVGRNKQ TYVTWQADCK ENAGGDYYWT 190 200 210 220 230 240 FFPQPTFVST QKYYCHVDNS CYMNFDFSAP EYHELALWED KATLRFECAD TYISLLEKLT 250 260 270 280 290 300 ALLGRQPELP DWIYDGVTLG IQGGTEVCQK KLDTMRNAGV KVNGIWAQDW SGIRMTSFGK 310 320 330 340 350 360 RVMWNWKWNS ENYPQLDSRI KQWNQEGVQF LAYINPYVAS DKDLCEEAAQ HGYLAKDASG 370 380 390 400 410 420 GDYLVEFGEF YGGVVDLTNP EAYAWFKEVI KKNMIELGCG GWMADFGEYL PTDTYLHNGV 430 440 450 460 470 480 SAEIMHNAWP ALWAKCNYEA LEETGKLGEI LFFMRAGSTG SQKYSTMMWA GDQNVDWSLD 490 500 510 520 530 540 DGLASVVPAA LSLAMTGHGL HHSDIGGYTT LFEMKRSKEL LLRWCDFSAF TPMMRTHEGN 550 560 570 580 590 600 RPGDNWQFDG DAETIAHFAR MTTVFTTLKP YLKEAVALNA KSGLPVMRPL FLHYEDDAHT 610 620 630 640 650 660 YTLKYQYLLG RDILVAPVHE EGRSDWTLYL PEDNWVHAWT GEAFRGGEVT VNAPIGKPPV 670 FYRADSEWAA LFASLKSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 358 AND 517. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli." Okuyama M., Mori H., Chiba S., Kimura A. Protein Expr. Purif. 37:170-179(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19201 Genomic DNA. Translation: AAB03011.1. U00096 Genomic DNA. Translation: AAC76875.1. AP009048 Genomic DNA. Translation: BAE77431.1.
PIR	A65193.
RefSeq	NP_418314.1. NC_000913.2. YP_491572.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P32138.
SMR	P32138. Positions 90-670.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12498N.
IntAct	P32138. 1 interaction.
MINT	MINT-1249337.
STRING	511145.b3878.
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76875; AAC76875; b3878. BAE77431; BAE77431; BAE77431.
GeneID	12931872. 948376.
KEGG	ecj:Y75_p3308. eco:b3878.
PATRIC	32123259. VBIEscCol129921_3990.
Organism-specific databases
EchoBASE	EB1789.
EcoGene	EG11843. yihQ.
Phylogenomic databases
eggNOG	COG1501.
HOGENOM	HOG000064244.
KO	K15922.
OMA	HELAFWE.
ProtClustDB	PRK10426.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11843-MONOMER. ECOL316407:JW3849-MONOMER.
Gene expression databases
Genevestigator	P32138.
Family and domain databases
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR000322. Glyco_hydro_31. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. PTHR22762. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. False negative. PS00707. GLYCOSYL_HYDROL_F31_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YIHQ_ECOLI

Accession

Primary (citable) accession number: P32138
Secondary accession number(s): P76775, Q2M8H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program