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P32138

- YIHQ_ECOLI

UniProt

P32138 - YIHQ_ECOLI

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Protein

Alpha-glucosidase YihQ

Gene

yihQ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Exhibits hydrolysis activity against alpha-glucosyl fluoride, although natural substrates, such as alpha-glucobioses are scarcely hydrolyzed.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051NucleophileBy similarity
Active sitei408 – 4081By similarity
Active sitei472 – 4721Proton donorBy similarity

GO - Molecular functioni

  1. alpha-1,4-glucosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG11843-MONOMER.
ECOL316407:JW3849-MONOMER.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase YihQ (EC:3.2.1.20)
Gene namesi
Name:yihQ
Ordered Locus Names:b3878, JW3849
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11843. yihQ.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 678678Alpha-glucosidase YihQPRO_0000185372Add
BLAST

Expressioni

Inductioni

Induced during growth with sulfoquinovose.1 Publication

Gene expression databases

GenevestigatoriP32138.

Interactioni

Protein-protein interaction databases

DIPiDIP-12498N.
IntActiP32138. 1 interaction.
MINTiMINT-1249337.
STRINGi511145.b3878.

Structurei

3D structure databases

ProteinModelPortaliP32138.
SMRiP32138. Positions 309-635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Phylogenomic databases

eggNOGiCOG1501.
HOGENOMiHOG000064244.
InParanoidiP32138.
KOiK15922.
OMAiKYQYLFG.
OrthoDBiEOG6Z99WN.
PhylomeDBiP32138.

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

P32138-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTPRPQLLD FQFHQNNDSF TLHFQQRLIL THSKDNPCLW IGSGIADIDM
60 70 80 90 100
FRGNFSIKDK LQEKIALTDA IVSQSPDGWL IHFSRGSDIS ATLNISADDQ
110 120 130 140 150
GRLLLELQND NLNHNRIWLR LAAQPEDHIY GCGEQFSYFD LRGKPFPLWT
160 170 180 190 200
SEQGVGRNKQ TYVTWQADCK ENAGGDYYWT FFPQPTFVST QKYYCHVDNS
210 220 230 240 250
CYMNFDFSAP EYHELALWED KATLRFECAD TYISLLEKLT ALLGRQPELP
260 270 280 290 300
DWIYDGVTLG IQGGTEVCQK KLDTMRNAGV KVNGIWAQDW SGIRMTSFGK
310 320 330 340 350
RVMWNWKWNS ENYPQLDSRI KQWNQEGVQF LAYINPYVAS DKDLCEEAAQ
360 370 380 390 400
HGYLAKDASG GDYLVEFGEF YGGVVDLTNP EAYAWFKEVI KKNMIELGCG
410 420 430 440 450
GWMADFGEYL PTDTYLHNGV SAEIMHNAWP ALWAKCNYEA LEETGKLGEI
460 470 480 490 500
LFFMRAGSTG SQKYSTMMWA GDQNVDWSLD DGLASVVPAA LSLAMTGHGL
510 520 530 540 550
HHSDIGGYTT LFEMKRSKEL LLRWCDFSAF TPMMRTHEGN RPGDNWQFDG
560 570 580 590 600
DAETIAHFAR MTTVFTTLKP YLKEAVALNA KSGLPVMRPL FLHYEDDAHT
610 620 630 640 650
YTLKYQYLLG RDILVAPVHE EGRSDWTLYL PEDNWVHAWT GEAFRGGEVT
660 670
VNAPIGKPPV FYRADSEWAA LFASLKSI
Length:678
Mass (Da):77,275
Last modified:July 15, 1998 - v3
Checksum:i85869F52BB72FEE7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti358 – 3581A → R in AAB03011. (PubMed:8346018)Curated
Sequence conflicti517 – 5171S → T in AAB03011. (PubMed:8346018)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19201 Genomic DNA. Translation: AAB03011.1.
U00096 Genomic DNA. Translation: AAC76875.1.
AP009048 Genomic DNA. Translation: BAE77431.1.
PIRiA65193.
RefSeqiNP_418314.1. NC_000913.3.
YP_491572.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76875; AAC76875; b3878.
BAE77431; BAE77431; BAE77431.
GeneIDi12931872.
948376.
KEGGiecj:Y75_p3308.
eco:b3878.
PATRICi32123259. VBIEscCol129921_3990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19201 Genomic DNA. Translation: AAB03011.1 .
U00096 Genomic DNA. Translation: AAC76875.1 .
AP009048 Genomic DNA. Translation: BAE77431.1 .
PIRi A65193.
RefSeqi NP_418314.1. NC_000913.3.
YP_491572.1. NC_007779.1.

3D structure databases

ProteinModelPortali P32138.
SMRi P32138. Positions 309-635.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12498N.
IntActi P32138. 1 interaction.
MINTi MINT-1249337.
STRINGi 511145.b3878.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76875 ; AAC76875 ; b3878 .
BAE77431 ; BAE77431 ; BAE77431 .
GeneIDi 12931872.
948376.
KEGGi ecj:Y75_p3308.
eco:b3878.
PATRICi 32123259. VBIEscCol129921_3990.

Organism-specific databases

EchoBASEi EB1789.
EcoGenei EG11843. yihQ.

Phylogenomic databases

eggNOGi COG1501.
HOGENOMi HOG000064244.
InParanoidi P32138.
KOi K15922.
OMAi KYQYLFG.
OrthoDBi EOG6Z99WN.
PhylomeDBi P32138.

Enzyme and pathway databases

BioCyci EcoCyc:EG11843-MONOMER.
ECOL316407:JW3849-MONOMER.

Miscellaneous databases

PROi P32138.

Gene expression databases

Genevestigatori P32138.

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 358 AND 517.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli."
    Okuyama M., Mori H., Chiba S., Kimura A.
    Protein Expr. Purif. 37:170-179(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Sulphoglycolysis in Escherichia coli K-12 closes a gap in the biogeochemical sulphur cycle."
    Denger K., Weiss M., Felux A.K., Schneider A., Mayer C., Spiteller D., Huhn T., Cook A.M., Schleheck D.
    Nature 507:114-117(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
    Strain: K12.

Entry informationi

Entry nameiYIHQ_ECOLI
AccessioniPrimary (citable) accession number: P32138
Secondary accession number(s): P76775, Q2M8H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3