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Protein

GTP-binding protein TypA/BipA

Gene

typA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably interacts with the ribosomes in a GTP dependent manner. GTPase that mediates interactions between enteropathogenic E.coli (EPEC) and epithelial cells.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi74 – 78GTPBy similarity5
Nucleotide bindingi128 – 131GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • response to cold Source: EcoCyc
  • response to heat Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11837-MONOMER.
ECOL316407:JW5571-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein TypA/BipA
Alternative name(s):
Tyrosine phosphorylated protein A
Gene namesi
Name:typA
Synonyms:bipA, yihK
Ordered Locus Names:b3871, JW5571
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11837. typA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915501 – 607GTP-binding protein TypA/BipAAdd BLAST607

Post-translational modificationi

Phosphorylated on tyrosine residues.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP32132.
PaxDbiP32132.
PRIDEiP32132.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rlmLP758643EBI-562154,EBI-547718

Protein-protein interaction databases

BioGridi4262631. 10 interactors.
DIPiDIP-11058N.
IntActiP32132. 5 interactors.
STRINGi511145.b3871.

Structurei

Secondary structure

1607
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 12Combined sources8
Helixi18 – 27Combined sources10
Beta strandi59 – 65Combined sources7
Beta strandi68 – 74Combined sources7
Beta strandi81 – 83Combined sources3
Helixi84 – 89Combined sources6
Beta strandi93 – 100Combined sources8
Turni101 – 103Combined sources3
Helixi107 – 109Combined sources3
Helixi110 – 118Combined sources9
Beta strandi123 – 128Combined sources6
Helixi137 – 147Combined sources11
Turni148 – 151Combined sources4
Helixi154 – 157Combined sources4
Beta strandi162 – 166Combined sources5
Turni167 – 170Combined sources4
Beta strandi171 – 175Combined sources5
Beta strandi181 – 183Combined sources3
Helixi184 – 193Combined sources10
Beta strandi200 – 203Combined sources4
Beta strandi206 – 208Combined sources3
Beta strandi211 – 215Combined sources5
Turni216 – 218Combined sources3
Beta strandi219 – 225Combined sources7
Beta strandi228 – 231Combined sources4
Beta strandi236 – 240Combined sources5
Beta strandi242 – 244Combined sources3
Beta strandi246 – 250Combined sources5
Beta strandi254 – 259Combined sources6
Beta strandi261 – 266Combined sources6
Beta strandi273 – 276Combined sources4
Beta strandi278 – 281Combined sources4
Beta strandi287 – 290Combined sources4
Beta strandi307 – 312Combined sources6
Turni317 – 320Combined sources4
Helixi328 – 338Combined sources11
Turni339 – 341Combined sources3
Beta strandi346 – 349Combined sources4
Beta strandi355 – 362Combined sources8
Helixi363 – 375Combined sources13
Beta strandi380 – 382Combined sources3
Beta strandi390 – 392Combined sources3
Beta strandi395 – 408Combined sources14
Helixi409 – 421Combined sources13
Beta strandi425 – 431Combined sources7
Beta strandi435 – 444Combined sources10
Helixi445 – 448Combined sources4
Helixi451 – 459Combined sources9
Beta strandi464 – 474Combined sources11
Beta strandi485 – 490Combined sources6
Helixi498 – 504Combined sources7
Helixi505 – 507Combined sources3
Beta strandi508 – 511Combined sources4
Beta strandi522 – 531Combined sources10
Beta strandi533 – 535Combined sources3
Helixi542 – 546Combined sources5
Helixi565 – 571Combined sources7
Beta strandi576 – 581Combined sources6
Beta strandi584 – 591Combined sources8
Helixi594 – 601Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZCIX-ray2.63A/B1-601[»]
4ZCKX-ray2.48A306-603[»]
4ZCLX-ray3.06A/B1-601[»]
4ZCMX-ray3.31A/B1-607[»]
5A9WX-ray3.70A1-607[»]
5A9XX-ray3.80A1-607[»]
5A9YX-ray4.00A1-607[»]
ProteinModelPortaliP32132.
SMRiP32132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 198tr-type GPROSITE-ProRule annotationAdd BLAST196

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. TypA subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CI3. Bacteria.
COG1217. LUCA.
HOGENOMiHOG000282351.
InParanoidiP32132.
KOiK06207.
OMAiSMLFTIN.
PhylomeDBiP32132.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR006298. TypA_GTP-bd.
[Graphical view]
PANTHERiPTHR23115:SF14. PTHR23115:SF14. 1 hit.
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
TIGR01394. TypA_BipA. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKLRNIAI IAHVDHGKTT LVDKLLQQSG TFDSRAETQE RVMDSNDLEK
60 70 80 90 100
ERGITILAKN TAIKWNDYRI NIVDTPGHAD FGGEVERVMS MVDSVLLVVD
110 120 130 140 150
AFDGPMPQTR FVTKKAFAYG LKPIVVINKV DRPGARPDWV VDQVFDLFVN
160 170 180 190 200
LDATDEQLDF PIVYASALNG IAGLDHEDMA EDMTPLYQAI VDHVPAPDVD
210 220 230 240 250
LDGPFQMQIS QLDYNSYVGV IGIGRIKRGK VKPNQQVTII DSEGKTRNAK
260 270 280 290 300
VGKVLGHLGL ERIETDLAEA GDIVAITGLG ELNISDTVCD TQNVEALPAL
310 320 330 340 350
SVDEPTVSMF FCVNTSPFCG KEGKFVTSRQ ILDRLNKELV HNVALRVEET
360 370 380 390 400
EDADAFRVSG RGELHLSVLI ENMRREGFEL AVSRPKVIFR EIDGRKQEPY
410 420 430 440 450
ENVTLDVEEQ HQGSVMQALG ERKGDLKNMN PDGKGRVRLD YVIPSRGLIG
460 470 480 490 500
FRSEFMTMTS GTGLLYSTFS HYDDVRPGEV GQRQNGVLIS NGQGKAVAFA
510 520 530 540 550
LFGLQDRGKL FLGHGAEVYE GQIIGIHSRS NDLTVNCLTG KKLTNMRASG
560 570 580 590 600
TDEAVVLVPP IRMTLEQALE FIDDDELVEV TPTSIRIRKR HLTENDRRRA

NRAPKDD
Length:607
Mass (Da):67,355
Last modified:October 11, 2004 - v2
Checksum:i3B4DE3A514F95FFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti592 – 607Missing (PubMed:9622352).CuratedAdd BLAST16
Sequence conflicti592 – 607Missing (PubMed:8346018).CuratedAdd BLAST16

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti489I → T in strain: MAR001. 1
Natural varianti497V → A in strain: MAR001. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224871 Genomic DNA. Translation: CAA12172.1.
L19201 Genomic DNA. Translation: AAB03005.1.
U00096 Genomic DNA. Translation: AAT48232.1.
AP009048 Genomic DNA. Translation: BAE77438.1.
PIRiS40816.
RefSeqiWP_000570668.1. NZ_LN832404.1.
YP_026274.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48232; AAT48232; b3871.
BAE77438; BAE77438; BAE77438.
GeneIDi948369.
KEGGiecj:JW5571.
eco:b3871.
PATRICi32123245. VBIEscCol129921_3983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224871 Genomic DNA. Translation: CAA12172.1.
L19201 Genomic DNA. Translation: AAB03005.1.
U00096 Genomic DNA. Translation: AAT48232.1.
AP009048 Genomic DNA. Translation: BAE77438.1.
PIRiS40816.
RefSeqiWP_000570668.1. NZ_LN832404.1.
YP_026274.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZCIX-ray2.63A/B1-601[»]
4ZCKX-ray2.48A306-603[»]
4ZCLX-ray3.06A/B1-601[»]
4ZCMX-ray3.31A/B1-607[»]
5A9WX-ray3.70A1-607[»]
5A9XX-ray3.80A1-607[»]
5A9YX-ray4.00A1-607[»]
ProteinModelPortaliP32132.
SMRiP32132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262631. 10 interactors.
DIPiDIP-11058N.
IntActiP32132. 5 interactors.
STRINGi511145.b3871.

Proteomic databases

EPDiP32132.
PaxDbiP32132.
PRIDEiP32132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48232; AAT48232; b3871.
BAE77438; BAE77438; BAE77438.
GeneIDi948369.
KEGGiecj:JW5571.
eco:b3871.
PATRICi32123245. VBIEscCol129921_3983.

Organism-specific databases

EchoBASEiEB1783.
EcoGeneiEG11837. typA.

Phylogenomic databases

eggNOGiENOG4105CI3. Bacteria.
COG1217. LUCA.
HOGENOMiHOG000282351.
InParanoidiP32132.
KOiK06207.
OMAiSMLFTIN.
PhylomeDBiP32132.

Enzyme and pathway databases

BioCyciEcoCyc:EG11837-MONOMER.
ECOL316407:JW5571-MONOMER.

Miscellaneous databases

PROiP32132.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR006298. TypA_GTP-bd.
[Graphical view]
PANTHERiPTHR23115:SF14. PTHR23115:SF14. 1 hit.
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
TIGR01394. TypA_BipA. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYPA_ECOLI
AccessioniPrimary (citable) accession number: P32132
Secondary accession number(s): Q2M8G8, Q6BEY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.