Reviewed, UniProtKB/Swiss-Prot P32121 (ARRB2_HUMAN)

Names and origin

Protein names

Recommended name:
    Beta-arrestin-2
Alternative name(s):
    Arrestin beta-2

Gene names

Name:	ARRB2
Synonyms:	ARB2, ARR2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulates beta-adrenergic receptor function. Beta-arrestins seem to bind phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the arrestin family.

Ontologies

Keywords
Biological process	Sensory transduction
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	receptor internalization Inferred from direct assay. Source: UniProtKB response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception Inferred from electronic annotation. Source: InterPro transforming growth factor beta receptor signaling pathway Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasmic vesicle Inferred from direct assay. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AP3B1	O00203	1	EBI-714559,EBI-1044383
ARRB1	P49407	1	EBI-714559,EBI-743313
BOP1	Q14137	1	EBI-714559,EBI-1050828
CALM1	P62158	1	EBI-714559,EBI-397435
DDX27	Q96GQ7	1	EBI-714559,EBI-722051
FBL	P22087	1	EBI-714559,EBI-358318
GSN	P06396	1	EBI-714559,EBI-351506
HSPA8	P11142	1	EBI-714559,EBI-351896
KPNA3	O00505	1	EBI-714559,EBI-358297
KPNA4	O00629	1	EBI-714559,EBI-396343
MRPL43	Q8N983	1	EBI-714559,EBI-1043145
MRPL44	Q9H9J2	1	EBI-714559,EBI-713619
NCL	P19338	1	EBI-714559,EBI-352553
NOLC1	Q14978	2	EBI-714559,EBI-396155
PES1	O00541	1	EBI-714559,EBI-1053271
PKM2	P14618	1	EBI-714559,EBI-353408
PPM1A	P35813	1	EBI-714559,EBI-989143
PPM1B	O75688	1	EBI-714559,EBI-1047039
PRPF4B	Q13523	1	EBI-714559,EBI-395940
RAB5C	P51148	1	EBI-714559,EBI-1054923
RPL7L1	Q6DKI1	1	EBI-714559,EBI-1052408
S100A9	P06702	1	EBI-714559,EBI-1055001
STK38	Q15208	1	EBI-714559,EBI-458376
TCOF1	Q13428	2	EBI-714559,EBI-396105
YWHAQ	P27348	1	EBI-714559,EBI-359854
ZRANB2	O95218	1	EBI-714559,EBI-1051583

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P32121-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P32121-3)

The sequence of this isoform differs from the canonical sequence as follows:
     360-360: S → SAPTPTPPLPVPP

Isoform 3 (identifier: P32121-2)

The sequence of this isoform differs from the canonical sequence as follows:
     39-53: Missing.
     360-360: S → SAPTPTPPLPVPP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Beta-arrestin-2		PRO_0000205199
Regions
	Motif	389 – 399	11	Nuclear export signal By similarity
Amino acid modifications
	Modified residue	48	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	39 – 53	15	Missing in isoform 3.		VSP_008194
	Alternative sequence	360	1	S → SAPTPTPPLPVPP in isoform 2 and isoform 3.		VSP_008195
Experimental info
	Sequence conflict	189	1	R → P in CAA77577. Ref.1
	Sequence conflict	190	1	H → R in ABG47460. Ref.3
	Sequence conflict	192	1	L → P in CAG29306. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: DEEC507D4A7B84FF	FASTA	409	46,106
	10 20 30 40 50 60 MGEKPGTRVF KKSSPNCKLT VYLGKRDFVD HLDKVDPVDG VVLVDPDYLK DRKVFVTLTC 70 80 90 100 110 120 AFRYGREDLD VLGLSFRKDL FIATYQAFPP VPNPPRPPTR LQDRLLRKLG QHAHPFFFTI 130 140 150 160 170 180 PQNLPCSVTL QPGPEDTGKA CGVDFEIRAF CAKSLEEKSH KRNSVRLVIR KVQFAPEKPG 190 200 210 220 230 240 PQPSAETTRH FLMSDRSLHL EASLDKELYY HGEPLNVNVH VTNNSTKTVK KIKVSVRQYA 250 260 270 280 290 300 DICLFSTAQY KCPVAQLEQD DQVSPSSTFC KVYTITPLLS DNREKRGLAL DGKLKHEDTN 310 320 330 340 350 360 LASSTIVKEG ANKEVLGILV SYRVKVKLVV SRGGDVSVEL PFVLMHPKPH DHIPLPRPQS 370 380 390 400 AAPETDVPVD TNLIEFDTNY ATDDDIVFED FARLRLKGMK DDDYDDQLC
	Isoform 2. Checksum: 1D5F728967E2BF5D	FASTA	421	47,271
	10 20 30 40 50 60 MGEKPGTRVF KKSSPNCKLT VYLGKRDFVD HLDKVDPVDG VVLVDPDYLK DRKVFVTLTC 70 80 90 100 110 120 AFRYGREDLD VLGLSFRKDL FIATYQAFPP VPNPPRPPTR LQDRLLRKLG QHAHPFFFTI 130 140 150 160 170 180 PQNLPCSVTL QPGPEDTGKA CGVDFEIRAF CAKSLEEKSH KRNSVRLVIR KVQFAPEKPG 190 200 210 220 230 240 PQPSAETTRH FLMSDRSLHL EASLDKELYY HGEPLNVNVH VTNNSTKTVK KIKVSVRQYA 250 260 270 280 290 300 DICLFSTAQY KCPVAQLEQD DQVSPSSTFC KVYTITPLLS DNREKRGLAL DGKLKHEDTN 310 320 330 340 350 360 LASSTIVKEG ANKEVLGILV SYRVKVKLVV SRGGDVSVEL PFVLMHPKPH DHIPLPRPQS 370 380 390 400 410 420 APTPTPPLPV PPAAPETDVP VDTNLIEFDT NYATDDDIVF EDFARLRLKG MKDDDYDDQL C
	Isoform 3. Checksum: 2020CE75484DF687	FASTA	406	45,557
	10 20 30 40 50 60 MGEKPGTRVF KKSSPNCKLT VYLGKRDFVD HLDKVDPVVF VTLTCAFRYG REDLDVLGLS 70 80 90 100 110 120 FRKDLFIATY QAFPPVPNPP RPPTRLQDRL LRKLGQHAHP FFFTIPQNLP CSVTLQPGPE 130 140 150 160 170 180 DTGKACGVDF EIRAFCAKSL EEKSHKRNSV RLVIRKVQFA PEKPGPQPSA ETTRHFLMSD 190 200 210 220 230 240 RSLHLEASLD KELYYHGEPL NVNVHVTNNS TKTVKKIKVS VRQYADICLF STAQYKCPVA 250 260 270 280 290 300 QLEQDDQVSP SSTFCKVYTI TPLLSDNREK RGLALDGKLK HEDTNLASST IVKEGANKEV 310 320 330 340 350 360 LGILVSYRVK VKLVVSRGGD VSVELPFVLM HPKPHDHIPL PRPQSAPTPT PPLPVPPAAP 370 380 390 400 ETDVPVDTNL IEFDTNYATD DDIVFEDFAR LRLKGMKDDD YDDQLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a member of the arrestin family from a human thyroid cDNA library." Rapoport B., Kaufman K.D., Chamenbalk G.D. Mol. Cell. Endocrinol. 84:R39-R43(1992) [PubMed: 1587386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Thyroid.
[2]	"G-protein coupled receptor interaction with beta-arrestin 2 through specific agonist stimulation." Yu Q.M., Zhou T.H., Wu Y.L., Cheng Z.J., Ma L., Pei G. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"A new splice-variant of beta-arrestin 2 is involved in agonist-induced MC1R endocytosis." Sanchez-Laorden B.L., Jimenez-Cervantes C., Garcia-Borron J.C. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	NHLBI resequencing and genotyping service (RS&G) Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle and Pancreas.
+	Additional computationally mapped references.

Web resources

Wikipedia

Arrestin entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11501 mRNA. Translation: CAA77577.1. AF106941 mRNA. Translation: AAC99468.1. DQ664180 mRNA. Translation: ABG47460.1. AK097542 mRNA. Translation: BAC05094.1. CR450310 mRNA. Translation: CAG29306.1. DQ314866 Genomic DNA. Translation: ABC40725.1. BC007427 mRNA. Translation: AAH07427.1. BC067368 mRNA. Translation: AAH67368.1.
IPI	IPI00180375. IPI00783893. IPI00786904.
PIR	S18984.
RefSeq	NP_004304.1. NP_945355.1.
UniGene	Hs.435811
3D structure databases
HSSP	HSSP built from PDB template 1G4M based on UniProtKB P17870.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32121. 276 interactions.
PTM databases
PhosphoSite	P32121.
Proteomic databases
PRIDE	P32121.
Genome annotation databases
Ensembl	ENSG00000141480. Homo sapiens. [Contig view]
GeneID	409.
KEGG	hsa:409.
Organism-specific databases
GeneCards	GC17P004560.
HGNC	HGNC:712. ARRB2.
MIM	107941. gene.
PharmGKB	PA60.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P32121.
OMA	P32121. KGMKDDD.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6arrestinpathway. Arf6 mediated densensitization of LHCGR. arf6cyclingpathway. Arf6 signaling events. arf6_traffickingpathway. Arf6 trafficking events. nfkappabatypicalpathway. Atypical NF-kappaB pathway. hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins. hedgehog_2pathway. Signaling events mediated by the Hedgehog family. tgfbrpathway. TGF-beta receptor signaling. txa2pathway. Thromboxane A2 receptor signaling.
Gene expression databases
ArrayExpress	P32121.
Bgee	P32121.
CleanEx	HS_ARRB2.
GermOnline	ENSG00000141480. Homo sapiens.
Family and domain databases
InterPro	IPR000698. Arrestin. IPR011022. Arrestin-like_C. IPR011021. Arrestin-like_N. IPR014752. Arrestin_C. IPR017864. Arrestin_CS. IPR014753. Arrestin_N. [Graphical view]
Gene3D	G3DSA:2.60.40.640. Arrestin_C. 1 hit. G3DSA:2.60.40.840. Arrestin_N. 1 hit.
PANTHER	PTHR11792. Arrestin. 1 hit.
Pfam	PF02752. Arrestin_C. 1 hit. PF00339. Arrestin_N. 1 hit. [Graphical view]
PRINTS	PR00309. ARRESTIN.
ProDom	PD002099. Arrestin. 2 hits. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00295. ARRESTINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	1719.
SOURCE	Search...

Entry information

Entry name

ARRB2_HUMAN

Accession

Primary (citable) accession number: P32121
Secondary accession number(s): Q0Z8D3 , Q2PP19, Q6ICT3, Q8N7Y2, Q9UEQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	March 5, 2002
Last modified:	June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)