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P32115

- PAX4_MOUSE

UniProt

P32115 - PAX4_MOUSE

Protein

Paired box protein Pax-4

Gene

Pax4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that competes with PAX6 in binding to a common element in the glucagon, insulin and somatostatin promoters.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi170 – 22960HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. double-stranded DNA binding Source: Ensembl
    3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
    4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    5. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. circadian rhythm Source: Ensembl
    3. endocrine pancreas development Source: Reactome
    4. negative regulation of apoptotic process Source: Ensembl
    5. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    6. organ morphogenesis Source: MGI
    7. positive regulation of cell differentiation Source: MGI
    8. regulation of cell differentiation Source: MGI
    9. regulation of transcription, DNA-templated Source: MGI
    10. response to cAMP Source: Ensembl
    11. response to drug Source: Ensembl
    12. retina development in camera-type eye Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_221082. Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Paired box protein Pax-4
    Gene namesi
    Name:Pax4
    Synonyms:Pax-4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:97488. Pax4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi272 – 2732EP → DL: Loss of transactivation function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Paired box protein Pax-4PRO_0000050181Add
    BLAST

    Proteomic databases

    PRIDEiP32115.

    Expressioni

    Tissue specificityi

    Expressed in early pancreas. Later restricted to beta cells. Undetectable in adult islets.

    Gene expression databases

    BgeeiP32115.
    CleanExiMM_PAX4.
    GenevestigatoriP32115.

    Structurei

    3D structure databases

    ProteinModelPortaliP32115.
    SMRiP32115. Positions 7-135, 164-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 131127PairedPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni278 – 34972Transcription repressionAdd
    BLAST

    Sequence similaritiesi

    Belongs to the paired homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 paired domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox, Paired box

    Phylogenomic databases

    eggNOGiNOG309473.
    GeneTreeiENSGT00680000099553.
    HOGENOMiHOG000230939.
    HOVERGENiHBG009115.
    InParanoidiP32115.
    KOiK08032.
    OMAiESLGPSC.
    OrthoDBiEOG783MVM.
    PhylomeDBiP32115.
    TreeFamiTF320146.

    Family and domain databases

    Gene3Di1.10.10.10. 2 hits.
    1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR001523. Paired_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00292. PAX. 1 hit.
    [Graphical view]
    PRINTSiPR00027. PAIREDBOX.
    SMARTiSM00389. HOX. 1 hit.
    SM00351. PAX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 2 hits.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00034. PAIRED_1. 1 hit.
    PS51057. PAIRED_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P32115-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQQDGLSSVN QLGGLFVNGR PLPLDTRQQI VQLAIRGMRP CDISRSLKVS    50
    NGCVSKILGR YYRTGVLEPK CIGGSKPRLA TPAVVARIAQ LKDEYPALFA 100
    WEIQHQLCTE GLCTQDKAPS VSSINRVLRA LQEDQSLHWT QLRSPAVLAP 150
    VLPSPHSNCG APRGPHPGTS HRNRTIFSPG QAEALEKEFQ RGQYPDSVAR 200
    GKLAAATSLP EDTVRVWFSN RRAKWRRQEK LKWEAQLPGA SQDLTVPKNS 250
    PGIISAQQSP GSVPSAALPV LEPLSPSFCQ LCCGTAPGRC SSDTSSQAYL 300
    QPYWDCQSLL PVASSSYVEF AWPCLTTHPV HHLIGGPGQV PSTHCSNWP 349
    Length:349
    Mass (Da):38,008
    Last modified:May 30, 2000 - v2
    Checksum:i215E0469F46DF49B
    GO
    Isoform 2 (identifier: P32115-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         239-251: Missing.

    Show »
    Length:336
    Mass (Da):36,713
    Checksum:i15B04846791607E4
    GO
    Isoform 3 (identifier: P32115-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         278-314: Missing.

    Show »
    Length:312
    Mass (Da):34,039
    Checksum:i9FA23A25741CB4A8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti175 – 1751T → A in CAA70772. (PubMed:9121556)Curated
    Sequence conflicti277 – 2771S → P in AAF14073. (PubMed:10567552)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei239 – 25113Missing in isoform 2. 2 PublicationsVSP_002361Add
    BLAST
    Alternative sequencei278 – 31437Missing in isoform 3. 1 PublicationVSP_002362Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031150 mRNA. Translation: AAC40046.1.
    AB008912 mRNA. Translation: BAA24505.1.
    AB010557 mRNA. Translation: BAA24516.1.
    AB010558 mRNA. Translation: BAA24517.1.
    AF145233 mRNA. Translation: AAF03533.1.
    AF142321 Genomic DNA. Translation: AAF03530.1.
    BC145168 mRNA. Translation: AAI45169.1.
    AF104231 mRNA. Translation: AAF14073.1.
    Y09584 mRNA. Translation: CAA70772.1.
    CCDSiCCDS39447.1. [P32115-1]
    CCDS51732.1. [P32115-2]
    PIRiA41061.
    JC5827.
    JC5961.
    JC5962.
    RefSeqiNP_001153397.1. NM_001159925.1. [P32115-2]
    NP_001153398.1. NM_001159926.1.
    NP_035168.1. NM_011038.2. [P32115-1]
    UniGeneiMm.8026.

    Genome annotation databases

    EnsembliENSMUST00000031718; ENSMUSP00000031718; ENSMUSG00000029706. [P32115-1]
    ENSMUST00000164519; ENSMUSP00000131301; ENSMUSG00000029706. [P32115-2]
    GeneIDi18506.
    KEGGimmu:18506.
    UCSCiuc009bcs.2. mouse. [P32115-1]
    uc012eit.1. mouse. [P32115-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031150 mRNA. Translation: AAC40046.1 .
    AB008912 mRNA. Translation: BAA24505.1 .
    AB010557 mRNA. Translation: BAA24516.1 .
    AB010558 mRNA. Translation: BAA24517.1 .
    AF145233 mRNA. Translation: AAF03533.1 .
    AF142321 Genomic DNA. Translation: AAF03530.1 .
    BC145168 mRNA. Translation: AAI45169.1 .
    AF104231 mRNA. Translation: AAF14073.1 .
    Y09584 mRNA. Translation: CAA70772.1 .
    CCDSi CCDS39447.1. [P32115-1 ]
    CCDS51732.1. [P32115-2 ]
    PIRi A41061.
    JC5827.
    JC5961.
    JC5962.
    RefSeqi NP_001153397.1. NM_001159925.1. [P32115-2 ]
    NP_001153398.1. NM_001159926.1.
    NP_035168.1. NM_011038.2. [P32115-1 ]
    UniGenei Mm.8026.

    3D structure databases

    ProteinModelPortali P32115.
    SMRi P32115. Positions 7-135, 164-241.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P32115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031718 ; ENSMUSP00000031718 ; ENSMUSG00000029706 . [P32115-1 ]
    ENSMUST00000164519 ; ENSMUSP00000131301 ; ENSMUSG00000029706 . [P32115-2 ]
    GeneIDi 18506.
    KEGGi mmu:18506.
    UCSCi uc009bcs.2. mouse. [P32115-1 ]
    uc012eit.1. mouse. [P32115-2 ]

    Organism-specific databases

    CTDi 5078.
    MGIi MGI:97488. Pax4.

    Phylogenomic databases

    eggNOGi NOG309473.
    GeneTreei ENSGT00680000099553.
    HOGENOMi HOG000230939.
    HOVERGENi HBG009115.
    InParanoidi P32115.
    KOi K08032.
    OMAi ESLGPSC.
    OrthoDBi EOG783MVM.
    PhylomeDBi P32115.
    TreeFami TF320146.

    Enzyme and pathway databases

    Reactomei REACT_221082. Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells.

    Miscellaneous databases

    NextBioi 294228.
    PROi P32115.
    SOURCEi Search...

    Gene expression databases

    Bgeei P32115.
    CleanExi MM_PAX4.
    Genevestigatori P32115.

    Family and domain databases

    Gene3Di 1.10.10.10. 2 hits.
    1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR001523. Paired_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00292. PAX. 1 hit.
    [Graphical view ]
    PRINTSi PR00027. PAIREDBOX.
    SMARTi SM00389. HOX. 1 hit.
    SM00351. PAX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 2 hits.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00034. PAIRED_1. 1 hit.
    PS51057. PAIRED_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of full-length cDNA of mouse PAX4 gene and identification of its human homologue."
      Inoue H., Nomiyama J., Nakai K., Matsutani A., Tanizawa Y., Oka Y.
      Biochem. Biophys. Res. Commun. 243:628-633(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
      Tissue: Insulinoma.
    2. "Molecular cloning of mouse paired-box-containing gene (Pax-4) from an islet beta cell line and deduced sequence of human Pax-4."
      Matsushita T., Yamaoka T., Otsuka S., Moritani M., Matsumoto T., Itakura M.
      Biochem. Biophys. Res. Commun. 242:176-180(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Insulinoma.
    3. "DNA binding and transactivating properties of the paired and homeobox protein Pax4."
      Kalousova A., Benes V., Paces J., Paces V., Kozmik Z.
      Biochem. Biophys. Res. Commun. 259:510-518(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF 272-GLU-PRO-273.
    4. "Identification of a portable repression domain and an E1A-responsive activation domain in Pax4: a possible role of Pax4 as a transcriptional repressor in the pancreas."
      Fujitani Y., Kajimoto Y., Yasuda T., Matsuoka T.-A., Kaneto H., Umayahara Y., Fujita N., Watada H., Miyazaki J., Yamasaki Y.
      Mol. Cell. Biol. 19:8281-8291(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Insulinoma.
    5. "Paired-homeodomain transcription factor PAX4 acts as a transcriptional repressor in early pancreatic development."
      Smith S.B., Ee H.C., Conners J.R., German M.S.
      Mol. Cell. Biol. 19:8272-8280(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Insulinoma.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    7. "Pax: a murine multigene family of paired box-containing genes."
      Walther C., Guenet J.-L., Simon D., Deutsch U., Jostes B., Goulding M.D., Plachov D., Balling R., Gruss P.
      Genomics 11:424-434(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 6-132.
    8. "The Pax4 gene is essential for differentiation of insulin-producing beta cells in the mammalian pancreas."
      Sosa-Pineda B., Chowdhury K., Torres M., Oliver G., Gruss P.
      Nature 386:399-402(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-237.
      Strain: NMRI.
      Tissue: Embryo.

    Entry informationi

    Entry nameiPAX4_MOUSE
    AccessioniPrimary (citable) accession number: P32115
    Secondary accession number(s): B7ZNC7
    , P97341, Q9QUR1, Q9R091
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3