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P32115

- PAX4_MOUSE

UniProt

P32115 - PAX4_MOUSE

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Protein

Paired box protein Pax-4

Gene

Pax4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that competes with PAX6 in binding to a common element in the glucagon, insulin and somatostatin promoters.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi170 – 22960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. double-stranded DNA binding Source: Ensembl
  3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
  4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  5. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. circadian rhythm Source: Ensembl
  3. endocrine pancreas development Source: Reactome
  4. negative regulation of apoptotic process Source: Ensembl
  5. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  6. organ morphogenesis Source: MGI
  7. positive regulation of cell differentiation Source: MGI
  8. regulation of cell differentiation Source: MGI
  9. regulation of transcription, DNA-templated Source: MGI
  10. response to cAMP Source: Ensembl
  11. response to drug Source: Ensembl
  12. retina development in camera-type eye Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_221082. Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Paired box protein Pax-4
Gene namesi
Name:Pax4
Synonyms:Pax-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:97488. Pax4.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi272 – 2732EP → DL: Loss of transactivation function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Paired box protein Pax-4PRO_0000050181Add
BLAST

Proteomic databases

PRIDEiP32115.

Expressioni

Tissue specificityi

Expressed in early pancreas. Later restricted to beta cells. Undetectable in adult islets.

Gene expression databases

BgeeiP32115.
CleanExiMM_PAX4.
GenevestigatoriP32115.

Structurei

3D structure databases

ProteinModelPortaliP32115.
SMRiP32115. Positions 7-135, 164-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 131127PairedPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni278 – 34972Transcription repressionAdd
BLAST

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 paired domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, Paired box

Phylogenomic databases

eggNOGiNOG309473.
GeneTreeiENSGT00680000099553.
HOGENOMiHOG000230939.
HOVERGENiHBG009115.
InParanoidiP32115.
KOiK08032.
OMAiESLGPSC.
OrthoDBiEOG783MVM.
PhylomeDBiP32115.
TreeFamiTF320146.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00292. PAX. 1 hit.
[Graphical view]
PRINTSiPR00027. PAIREDBOX.
SMARTiSM00389. HOX. 1 hit.
SM00351. PAX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P32115-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQDGLSSVN QLGGLFVNGR PLPLDTRQQI VQLAIRGMRP CDISRSLKVS
60 70 80 90 100
NGCVSKILGR YYRTGVLEPK CIGGSKPRLA TPAVVARIAQ LKDEYPALFA
110 120 130 140 150
WEIQHQLCTE GLCTQDKAPS VSSINRVLRA LQEDQSLHWT QLRSPAVLAP
160 170 180 190 200
VLPSPHSNCG APRGPHPGTS HRNRTIFSPG QAEALEKEFQ RGQYPDSVAR
210 220 230 240 250
GKLAAATSLP EDTVRVWFSN RRAKWRRQEK LKWEAQLPGA SQDLTVPKNS
260 270 280 290 300
PGIISAQQSP GSVPSAALPV LEPLSPSFCQ LCCGTAPGRC SSDTSSQAYL
310 320 330 340
QPYWDCQSLL PVASSSYVEF AWPCLTTHPV HHLIGGPGQV PSTHCSNWP
Length:349
Mass (Da):38,008
Last modified:May 30, 2000 - v2
Checksum:i215E0469F46DF49B
GO
Isoform 2 (identifier: P32115-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-251: Missing.

Show »
Length:336
Mass (Da):36,713
Checksum:i15B04846791607E4
GO
Isoform 3 (identifier: P32115-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-314: Missing.

Show »
Length:312
Mass (Da):34,039
Checksum:i9FA23A25741CB4A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751T → A in CAA70772. (PubMed:9121556)Curated
Sequence conflicti277 – 2771S → P in AAF14073. (PubMed:10567552)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei239 – 25113Missing in isoform 2. 2 PublicationsVSP_002361Add
BLAST
Alternative sequencei278 – 31437Missing in isoform 3. 1 PublicationVSP_002362Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031150 mRNA. Translation: AAC40046.1.
AB008912 mRNA. Translation: BAA24505.1.
AB010557 mRNA. Translation: BAA24516.1.
AB010558 mRNA. Translation: BAA24517.1.
AF145233 mRNA. Translation: AAF03533.1.
AF142321 Genomic DNA. Translation: AAF03530.1.
BC145168 mRNA. Translation: AAI45169.1.
AF104231 mRNA. Translation: AAF14073.1.
Y09584 mRNA. Translation: CAA70772.1.
CCDSiCCDS39447.1. [P32115-1]
CCDS51732.1. [P32115-2]
PIRiA41061.
JC5827.
JC5961.
JC5962.
RefSeqiNP_001153397.1. NM_001159925.1. [P32115-2]
NP_001153398.1. NM_001159926.1.
NP_035168.1. NM_011038.2. [P32115-1]
UniGeneiMm.8026.

Genome annotation databases

EnsembliENSMUST00000031718; ENSMUSP00000031718; ENSMUSG00000029706. [P32115-1]
ENSMUST00000164519; ENSMUSP00000131301; ENSMUSG00000029706. [P32115-2]
GeneIDi18506.
KEGGimmu:18506.
UCSCiuc009bcs.2. mouse. [P32115-1]
uc012eit.1. mouse. [P32115-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031150 mRNA. Translation: AAC40046.1 .
AB008912 mRNA. Translation: BAA24505.1 .
AB010557 mRNA. Translation: BAA24516.1 .
AB010558 mRNA. Translation: BAA24517.1 .
AF145233 mRNA. Translation: AAF03533.1 .
AF142321 Genomic DNA. Translation: AAF03530.1 .
BC145168 mRNA. Translation: AAI45169.1 .
AF104231 mRNA. Translation: AAF14073.1 .
Y09584 mRNA. Translation: CAA70772.1 .
CCDSi CCDS39447.1. [P32115-1 ]
CCDS51732.1. [P32115-2 ]
PIRi A41061.
JC5827.
JC5961.
JC5962.
RefSeqi NP_001153397.1. NM_001159925.1. [P32115-2 ]
NP_001153398.1. NM_001159926.1.
NP_035168.1. NM_011038.2. [P32115-1 ]
UniGenei Mm.8026.

3D structure databases

ProteinModelPortali P32115.
SMRi P32115. Positions 7-135, 164-241.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P32115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031718 ; ENSMUSP00000031718 ; ENSMUSG00000029706 . [P32115-1 ]
ENSMUST00000164519 ; ENSMUSP00000131301 ; ENSMUSG00000029706 . [P32115-2 ]
GeneIDi 18506.
KEGGi mmu:18506.
UCSCi uc009bcs.2. mouse. [P32115-1 ]
uc012eit.1. mouse. [P32115-2 ]

Organism-specific databases

CTDi 5078.
MGIi MGI:97488. Pax4.

Phylogenomic databases

eggNOGi NOG309473.
GeneTreei ENSGT00680000099553.
HOGENOMi HOG000230939.
HOVERGENi HBG009115.
InParanoidi P32115.
KOi K08032.
OMAi ESLGPSC.
OrthoDBi EOG783MVM.
PhylomeDBi P32115.
TreeFami TF320146.

Enzyme and pathway databases

Reactomei REACT_221082. Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells.

Miscellaneous databases

NextBioi 294228.
PROi P32115.
SOURCEi Search...

Gene expression databases

Bgeei P32115.
CleanExi MM_PAX4.
Genevestigatori P32115.

Family and domain databases

Gene3Di 1.10.10.10. 2 hits.
1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00292. PAX. 1 hit.
[Graphical view ]
PRINTSi PR00027. PAIREDBOX.
SMARTi SM00389. HOX. 1 hit.
SM00351. PAX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 2 hits.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of full-length cDNA of mouse PAX4 gene and identification of its human homologue."
    Inoue H., Nomiyama J., Nakai K., Matsutani A., Tanizawa Y., Oka Y.
    Biochem. Biophys. Res. Commun. 243:628-633(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Insulinoma.
  2. "Molecular cloning of mouse paired-box-containing gene (Pax-4) from an islet beta cell line and deduced sequence of human Pax-4."
    Matsushita T., Yamaoka T., Otsuka S., Moritani M., Matsumoto T., Itakura M.
    Biochem. Biophys. Res. Commun. 242:176-180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Insulinoma.
  3. "DNA binding and transactivating properties of the paired and homeobox protein Pax4."
    Kalousova A., Benes V., Paces J., Paces V., Kozmik Z.
    Biochem. Biophys. Res. Commun. 259:510-518(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF 272-GLU-PRO-273.
  4. "Identification of a portable repression domain and an E1A-responsive activation domain in Pax4: a possible role of Pax4 as a transcriptional repressor in the pancreas."
    Fujitani Y., Kajimoto Y., Yasuda T., Matsuoka T.-A., Kaneto H., Umayahara Y., Fujita N., Watada H., Miyazaki J., Yamasaki Y.
    Mol. Cell. Biol. 19:8281-8291(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Insulinoma.
  5. "Paired-homeodomain transcription factor PAX4 acts as a transcriptional repressor in early pancreatic development."
    Smith S.B., Ee H.C., Conners J.R., German M.S.
    Mol. Cell. Biol. 19:8272-8280(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Insulinoma.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  7. "Pax: a murine multigene family of paired box-containing genes."
    Walther C., Guenet J.-L., Simon D., Deutsch U., Jostes B., Goulding M.D., Plachov D., Balling R., Gruss P.
    Genomics 11:424-434(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 6-132.
  8. "The Pax4 gene is essential for differentiation of insulin-producing beta cells in the mammalian pancreas."
    Sosa-Pineda B., Chowdhury K., Torres M., Oliver G., Gruss P.
    Nature 386:399-402(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-237.
    Strain: NMRI.
    Tissue: Embryo.

Entry informationi

Entry nameiPAX4_MOUSE
AccessioniPrimary (citable) accession number: P32115
Secondary accession number(s): B7ZNC7
, P97341, Q9QUR1, Q9R091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: October 1, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3