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P32115 (PAX4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Paired box protein Pax-4
Gene names
Name:Pax4
Synonyms:Pax-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that competes with PAX6 in binding to a common element in the glucagon, insulin and somatostatin promoters.

Subcellular location

Nucleus.

Tissue specificity

Expressed in early pancreas. Later restricted to beta cells. Undetectable in adult islets.

Sequence similarities

Belongs to the paired homeobox family.

Contains 1 homeobox DNA-binding domain.

Contains 1 paired domain.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
Paired box
   LigandDNA-binding
   Molecular functionDevelopmental protein
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

circadian rhythm

Inferred from electronic annotation. Source: Ensembl

endocrine pancreas development

Traceable author statement. Source: Reactome

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.5. Source: MGI

organ morphogenesis

Traceable author statement PubMed 10197584. Source: MGI

positive regulation of cell differentiation

Inferred from mutant phenotype PubMed 14729487. Source: MGI

regulation of cell differentiation

Inferred from mutant phenotype PubMed 14970313. Source: MGI

regulation of transcription, DNA-templated

Traceable author statement PubMed 10197584. Source: MGI

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

retina development in camera-type eye

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleoplasm

Traceable author statement. Source: Reactome

transcription factor complex

Traceable author statement PubMed 10197584. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay Ref.5. Source: MGI

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 10197584. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P32115-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P32115-2)

The sequence of this isoform differs from the canonical sequence as follows:
     239-251: Missing.
Isoform 3 (identifier: P32115-3)

The sequence of this isoform differs from the canonical sequence as follows:
     278-314: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Paired box protein Pax-4
PRO_0000050181

Regions

Domain5 – 131127Paired
DNA binding170 – 22960Homeobox
Region278 – 34972Transcription repression

Natural variations

Alternative sequence239 – 25113Missing in isoform 2.
VSP_002361
Alternative sequence278 – 31437Missing in isoform 3.
VSP_002362

Experimental info

Mutagenesis272 – 2732EP → DL: Loss of transactivation function. Ref.3
Sequence conflict1751T → A in CAA70772. Ref.8
Sequence conflict2771S → P in AAF14073. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 215E0469F46DF49B

FASTA34938,008
        10         20         30         40         50         60 
MQQDGLSSVN QLGGLFVNGR PLPLDTRQQI VQLAIRGMRP CDISRSLKVS NGCVSKILGR 

        70         80         90        100        110        120 
YYRTGVLEPK CIGGSKPRLA TPAVVARIAQ LKDEYPALFA WEIQHQLCTE GLCTQDKAPS 

       130        140        150        160        170        180 
VSSINRVLRA LQEDQSLHWT QLRSPAVLAP VLPSPHSNCG APRGPHPGTS HRNRTIFSPG 

       190        200        210        220        230        240 
QAEALEKEFQ RGQYPDSVAR GKLAAATSLP EDTVRVWFSN RRAKWRRQEK LKWEAQLPGA 

       250        260        270        280        290        300 
SQDLTVPKNS PGIISAQQSP GSVPSAALPV LEPLSPSFCQ LCCGTAPGRC SSDTSSQAYL 

       310        320        330        340 
QPYWDCQSLL PVASSSYVEF AWPCLTTHPV HHLIGGPGQV PSTHCSNWP 

« Hide

Isoform 2 [UniParc].

Checksum: 15B04846791607E4
Show »

FASTA33636,713
Isoform 3 [UniParc].

Checksum: 9FA23A25741CB4A8
Show »

FASTA31234,039

References

« Hide 'large scale' references
[1]"Isolation of full-length cDNA of mouse PAX4 gene and identification of its human homologue."
Inoue H., Nomiyama J., Nakai K., Matsutani A., Tanizawa Y., Oka Y.
Biochem. Biophys. Res. Commun. 243:628-633(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Insulinoma.
[2]"Molecular cloning of mouse paired-box-containing gene (Pax-4) from an islet beta cell line and deduced sequence of human Pax-4."
Matsushita T., Yamaoka T., Otsuka S., Moritani M., Matsumoto T., Itakura M.
Biochem. Biophys. Res. Commun. 242:176-180(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Insulinoma.
[3]"DNA binding and transactivating properties of the paired and homeobox protein Pax4."
Kalousova A., Benes V., Paces J., Paces V., Kozmik Z.
Biochem. Biophys. Res. Commun. 259:510-518(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF 272-GLU-PRO-273.
[4]"Identification of a portable repression domain and an E1A-responsive activation domain in Pax4: a possible role of Pax4 as a transcriptional repressor in the pancreas."
Fujitani Y., Kajimoto Y., Yasuda T., Matsuoka T.-A., Kaneto H., Umayahara Y., Fujita N., Watada H., Miyazaki J., Yamasaki Y.
Mol. Cell. Biol. 19:8281-8291(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Insulinoma.
[5]"Paired-homeodomain transcription factor PAX4 acts as a transcriptional repressor in early pancreatic development."
Smith S.B., Ee H.C., Conners J.R., German M.S.
Mol. Cell. Biol. 19:8272-8280(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Insulinoma.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[7]"Pax: a murine multigene family of paired box-containing genes."
Walther C., Guenet J.-L., Simon D., Deutsch U., Jostes B., Goulding M.D., Plachov D., Balling R., Gruss P.
Genomics 11:424-434(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 6-132.
[8]"The Pax4 gene is essential for differentiation of insulin-producing beta cells in the mammalian pancreas."
Sosa-Pineda B., Chowdhury K., Torres M., Oliver G., Gruss P.
Nature 386:399-402(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-237.
Strain: NMRI.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031150 mRNA. Translation: AAC40046.1.
AB008912 mRNA. Translation: BAA24505.1.
AB010557 mRNA. Translation: BAA24516.1.
AB010558 mRNA. Translation: BAA24517.1.
AF145233 mRNA. Translation: AAF03533.1.
AF142321 Genomic DNA. Translation: AAF03530.1.
BC145168 mRNA. Translation: AAI45169.1.
AF104231 mRNA. Translation: AAF14073.1.
Y09584 mRNA. Translation: CAA70772.1.
CCDSCCDS39447.1. [P32115-1]
CCDS51732.1. [P32115-2]
PIRA41061.
JC5827.
JC5961.
JC5962.
RefSeqNP_001153397.1. NM_001159925.1. [P32115-2]
NP_001153398.1. NM_001159926.1.
NP_035168.1. NM_011038.2. [P32115-1]
UniGeneMm.8026.

3D structure databases

ProteinModelPortalP32115.
SMRP32115. Positions 7-135, 164-241.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP32115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031718; ENSMUSP00000031718; ENSMUSG00000029706. [P32115-1]
ENSMUST00000164519; ENSMUSP00000131301; ENSMUSG00000029706. [P32115-2]
GeneID18506.
KEGGmmu:18506.
UCSCuc009bcs.2. mouse. [P32115-1]
uc012eit.1. mouse. [P32115-2]

Organism-specific databases

CTD5078.
MGIMGI:97488. Pax4.

Phylogenomic databases

eggNOGNOG309473.
GeneTreeENSGT00680000099553.
HOGENOMHOG000230939.
HOVERGENHBG009115.
InParanoidP32115.
KOK08032.
OMAESLGPSC.
OrthoDBEOG783MVM.
PhylomeDBP32115.
TreeFamTF320146.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.

Gene expression databases

BgeeP32115.
CleanExMM_PAX4.
GenevestigatorP32115.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00292. PAX. 1 hit.
[Graphical view]
PRINTSPR00027. PAIREDBOX.
SMARTSM00389. HOX. 1 hit.
SM00351. PAX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 2 hits.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294228.
PROP32115.
SOURCESearch...

Entry information

Entry namePAX4_MOUSE
AccessionPrimary (citable) accession number: P32115
Secondary accession number(s): B7ZNC7 expand/collapse secondary AC list , P97341, Q9QUR1, Q9R091
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot