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Protein

Paired box protein Pax-2

Gene

Pax2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that may have a role in kidney cell differentiation.By similarity

GO - Molecular functioni

  • C2H2 zinc finger domain binding Source: UniProtKB
  • core promoter proximal region sequence-specific DNA binding Source: UniProtKB
  • DNA binding Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • superoxide-generating NADPH oxidase activity Source: UniProtKB
  • transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor binding Source: MGI
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • brain morphogenesis Source: DFLAT
  • branching involved in ureteric bud morphogenesis Source: MGI
  • camera-type eye development Source: MGI
  • cell fate determination Source: MGI
  • cellular response to glucose stimulus Source: UniProtKB
  • cellular response to hydrogen peroxide Source: UniProtKB
  • cellular response to mechanical stimulus Source: UniProtKB
  • cellular response to retinoic acid Source: UniProtKB
  • central nervous system development Source: UniProtKB
  • cochlea development Source: DFLAT
  • cochlea morphogenesis Source: UniProtKB
  • dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
  • glial cell differentiation Source: DFLAT
  • inner ear morphogenesis Source: UniProtKB
  • kidney development Source: MGI
  • mesenchymal to epithelial transition Source: MGI
  • mesenchymal to epithelial transition involved in metanephros morphogenesis Source: UniProtKB
  • mesodermal cell fate specification Source: UniProtKB
  • mesonephric duct development Source: UniProtKB
  • mesonephric tubule development Source: UniProtKB
  • mesonephric tubule formation Source: UniProtKB
  • mesonephros development Source: UniProtKB
  • metanephric collecting duct development Source: UniProtKB
  • metanephric distal convoluted tubule development Source: UniProtKB
  • metanephric epithelium development Source: UniProtKB
  • metanephric mesenchymal cell differentiation Source: UniProtKB
  • metanephric mesenchyme development Source: UniProtKB
  • metanephric nephron tubule formation Source: UniProtKB
  • metanephros development Source: MGI
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of apoptotic process involved in metanephric collecting duct development Source: UniProtKB
  • negative regulation of apoptotic process involved in metanephric nephron tubule development Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • negative regulation of cytolysis Source: UniProtKB
  • negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis Source: UniProtKB
  • negative regulation of mesenchymal cell apoptotic process involved in metanephros development Source: UniProtKB
  • negative regulation of programmed cell death Source: MGI
  • negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • nephric duct formation Source: UniProtKB
  • neural tube closure Source: DFLAT
  • optic chiasma development Source: DFLAT
  • optic cup morphogenesis involved in camera-type eye development Source: DFLAT
  • optic nerve development Source: DFLAT
  • optic nerve morphogenesis Source: DFLAT
  • optic nerve structural organization Source: DFLAT
  • pancreas development Source: UniProtKB
  • paramesonephric duct development Source: UniProtKB
  • positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
  • positive regulation of epithelial cell proliferation Source: MGI
  • positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Source: UniProtKB
  • positive regulation of metanephric DCT cell differentiation Source: UniProtKB
  • positive regulation of metanephric glomerulus development Source: UniProtKB
  • positive regulation of optic nerve formation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • pronephric field specification Source: UniProtKB
  • pronephros development Source: UniProtKB
  • protein kinase B signaling Source: UniProtKB
  • reactive oxygen species metabolic process Source: UniProtKB
  • regulation of apoptotic process Source: UniProtKB
  • regulation of metanephric nephron tubule epithelial cell differentiation Source: UniProtKB
  • regulation of metanephros size Source: UniProtKB
  • regulation of programmed cell death Source: MGI
  • renal system development Source: MGI
  • retinal pigment epithelium development Source: DFLAT
  • stem cell differentiation Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB
  • ureter development Source: UniProtKB
  • ureteric bud development Source: MGI
  • ureter maturation Source: UniProtKB
  • ureter morphogenesis Source: UniProtKB
  • urogenital system development Source: UniProtKB
  • vestibulocochlear nerve formation Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Paired box protein Pax-2
Gene namesi
Name:Pax2
Synonyms:Pax-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97486. Pax2.

Subcellular locationi

GO - Cellular componenti

  • centriolar satellite Source: UniProtKB
  • Golgi apparatus Source: MGI
  • microtubule organizing center Source: UniProtKB
  • nucleolus Source: MGI
  • nucleus Source: UniProtKB
  • protein complex Source: UniProtKB
  • protein-DNA complex Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Pax2 are a cause of developmental defects of the kidney, brain, ear and eye.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Paired box protein Pax-2PRO_0000050176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32114.
PRIDEiP32114.

PTM databases

iPTMnetiP32114.
PhosphoSiteiP32114.

Expressioni

Tissue specificityi

Kidney and nephrogenic rests.

Developmental stagei

Expressed in the developing embryo excretory and central nervous systems. In the developing excretory system; expressed in the pronephric tubules and extending nephric duct beginning at day 9 gestation. At 10 dpc, detected in the nephric cord, Wolffian duct and also individual pronephric tubules. At 11 dpc, expressed in the branched ureter and the mesenchymal condensations, at 12 dpc, expression seen in the collecting ducts and condensed mesenchymal cells. At 14-17 dpc, expressed in the perimeter of the growing kidney but expression declines in the more developed glomerular crevices, and at later developmental stages expression in the kidney declines. In the developing CNS; initially expressed in the ventricular zone in two compartments of cells on either side of the sulcus limitans and along the entire rhombencephalon and spinal cord, later is restricted to progeny cells that have migrated to specific regions of the intermediate zone. In the eye, expression is restricted to the ventral half of the optic cup and stalk and later to the optic disk and nerve. In the ear, expression is restricted to regions of the otic vesicle that form neuronal components.1 Publication

Gene expression databases

BgeeiP32114.
CleanExiMM_PAX2.

Interactioni

Subunit structurei

Interacts with ELGN3; the interaction targets PAX2 for destruction. Interacts with TLE4.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Paxip1Q6NZQ43EBI-1395232,EBI-1395317

GO - Molecular functioni

  • C2H2 zinc finger domain binding Source: UniProtKB
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi202029. 1 interaction.
IntActiP32114. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP32114.
SMRiP32114. Positions 18-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 141127PairedPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 paired domain.PROSITE-ProRule annotation

Keywords - Domaini

Paired box

Phylogenomic databases

HOGENOMiHOG000230938.
HOVERGENiHBG009115.
InParanoidiP32114.
KOiK15608.
OrthoDBiEOG7WT431.
PhylomeDBiP32114.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR022130. Pax2_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00292. PAX. 1 hit.
PF12403. Pax2_C. 1 hit.
[Graphical view]
PRINTSiPR00027. PAIREDBOX.
SMARTiSM00351. PAX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P32114-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMHCKADPF SAMHRHGGVN QLGGVFVNGR PLPDVVRQRI VELAHQGVRP
60 70 80 90 100
CDISRQLRVS HGCVSKILGR YYETGSIKPG VIGGSKPKVA TPKVVDKIAE
110 120 130 140 150
YKRQNPTMFA WEIRDRLLAE GICDNDTVPS VSSINRIIRT KVQQPFHPTP
160 170 180 190 200
DGAGTGVTAP GHTIVPSTAS PPVSSASNDP VGSYSINGIL GIPRSNGEKR
210 220 230 240 250
KREEVEVYTD PAHIRGGGGL HLVWTLRDVS EGSVPNGDSQ SGVDSLRKHL
260 270 280 290 300
RADTFTQQQL EALDRVFERP SYPDVFQASE HIKSEQGNEY SLPALTPGLD
310 320 330 340 350
EVKSSLSASA NPELGSNVSG TQTYPVVTGR DMTSTTLPGY PPHVPPTGQG
360 370 380 390 400
SYPTSTLAGM VPGSEFSGNP YSHPQYTAYN EAWRFSNPAL LSSPYYYSAA
410
PRGSAPAAAA YDRH
Length:414
Mass (Da):44,580
Last modified:September 2, 2008 - v2
Checksum:i7BADF256609F4811
GO
Isoform Short (identifier: P32114-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     205-227: Missing.

Show »
Length:391
Mass (Da):42,051
Checksum:iDA826493D7AE910E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151R → PG in AAI48233 (PubMed:1516825).Curated
Sequence conflicti15 – 151R → PG in AAI50485 (PubMed:1516825).Curated
Sequence conflicti115 – 1162DR → AQ in CAA39302 (PubMed:1977574).Curated
Sequence conflicti119 – 1191A → R in CAA39302 (PubMed:1977574).Curated
Sequence conflicti344 – 3441V → L in CAA39302 (PubMed:1977574).Curated
Sequence conflicti403 – 4075GSAPA → SAPAAR in CAA39302 (PubMed:1977574).Curated
Sequence conflicti410 – 4101A → AAA in AAI48233 (PubMed:1516825).Curated
Sequence conflicti410 – 4101A → AAA in AAI50485 (PubMed:1516825).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei205 – 22723Missing in isoform Short. 1 PublicationVSP_002347Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55781 Genomic DNA. Translation: CAA39302.1. Sequence problems.
BC148232 mRNA. Translation: AAI48233.1.
BC150484 mRNA. Translation: AAI50485.1.
PIRiA60086.
RefSeqiNP_035167.4. NM_011037.4.
UniGeneiMm.192158.

Genome annotation databases

GeneIDi18504.
KEGGimmu:18504.
UCSCiuc008hqa.2. mouse. [P32114-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55781 Genomic DNA. Translation: CAA39302.1. Sequence problems.
BC148232 mRNA. Translation: AAI48233.1.
BC150484 mRNA. Translation: AAI50485.1.
PIRiA60086.
RefSeqiNP_035167.4. NM_011037.4.
UniGeneiMm.192158.

3D structure databases

ProteinModelPortaliP32114.
SMRiP32114. Positions 18-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202029. 1 interaction.
IntActiP32114. 1 interaction.

PTM databases

iPTMnetiP32114.
PhosphoSiteiP32114.

Proteomic databases

MaxQBiP32114.
PRIDEiP32114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18504.
KEGGimmu:18504.
UCSCiuc008hqa.2. mouse. [P32114-2]

Organism-specific databases

CTDi5076.
MGIiMGI:97486. Pax2.

Phylogenomic databases

HOGENOMiHOG000230938.
HOVERGENiHBG009115.
InParanoidiP32114.
KOiK15608.
OrthoDBiEOG7WT431.
PhylomeDBiP32114.

Miscellaneous databases

PROiP32114.
SOURCEiSearch...

Gene expression databases

BgeeiP32114.
CleanExiMM_PAX2.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR022130. Pax2_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00292. PAX. 1 hit.
PF12403. Pax2_C. 1 hit.
[Graphical view]
PRINTSiPR00027. PAIREDBOX.
SMARTiSM00351. PAX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pax2, a new murine paired-box-containing gene and its expression in the developing excretory system."
    Dressler G.R., Deutsch U., Chowdhury K., Nornes H.O., Gruss P.
    Development 109:787-795(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Embryo.
  2. "Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis."
    Adams B., Doerfler P., Aguzzi A., Kozmik Z., Urbanek P., Maurer-Fogy I., Busslinger M.
    Genes Dev. 6:1589-1607(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 114-115 AND 118.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
  4. "Spatially and temporally restricted expression of Pax2 during murine neurogenesis."
    Nornes H.O., Dressler G.R., Knapik E.W., Deutsch U., Gruss P.
    Development 109:797-809(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "The mouse Pax2(1Neu) mutation is identical to a human PAX2 mutation in a family with renal-coloboma syndrome and results in developmental defects of the brain, ear, eye, and kidney."
    Favor J., Sandulache R., Neuhauser-Klaus A., Pretsch W., Chatterjee B., Senft E., Wurst W., Blanquet V., Grimes P., Sporle R., Schughart K.
    Proc. Natl. Acad. Sci. U.S.A. 93:13870-13875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISEASE.

Entry informationi

Entry nameiPAX2_MOUSE
AccessioniPrimary (citable) accession number: P32114
Secondary accession number(s): A7E2R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 2, 2008
Last modified: July 6, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.