P32110 (GSTX6_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione S-transferase
EC=2.5.1.18

Alternative name(s):
G2-4
Heat shock protein 26A

Gene names

Name:

HSP26-A

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in the cellular response to stress.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Induction	By heat shock and auxin; by heavy metals like cadmium, silver and copper.
Sequence similarities	Belongs to the GST superfamily. HSP26 family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Stress response
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Probable glutathione S-transferase		PRO_0000185871
Regions
	Domain	6 – 85	80	GST N-terminal
	Domain	90 – 214	125	GST C-terminal
	Region	69 – 70	2	Glutathione binding By similarity
Sites
	Binding site	16	1	Glutathione By similarity
	Binding site	43	1	Glutathione By similarity
	Binding site	57	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity
Experimental info
	Sequence conflict	216	1	A → E in AAA33943. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32110 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 9E89AC46A312E240	FASTA	225	26,011
	10 20 30 40 50 60 MAATQEDVKL LGIVGSPFVC RVQIALKLKG VEYKFLEENL GNKSDLLLKY NPVHKKVPVF 70 80 90 100 110 120 VHNEQPIAES LVIVEYIDET WKNNPILPSD PYQRALARFW SKFIDDKIVG AVSKSVFTVD 130 140 150 160 170 180 EKEREKNVEE TYEALQFLEN ELKDKKFFGG EEFGLVDIAA VFIAFWIPIF QEIAGLQLFT 190 200 210 220 SEKFPILYKW SQEFLNHPFV HEVLPPRDPL FAYFKARYES LSASK

References

[1]	"Characterization of Gmhsp26-A, a stress gene encoding a divergent heat shock protein of soybean: heavy-metal-induced inhibition of intron processing." Czarnecka E., Nagao R.T., Key J.L., Gurley W.B. Mol. Cell. Biol. 8:1113-1122(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Regulation of expression of an auxin-induced soybean sequence by cadmium." Hagen G., Uhrhammer N., Guilfoyle T.J. J. Biol. Chem. 263:6442-6446(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 86-225.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20363 Genomic DNA. Translation: AAA33973.1. J03197 Genomic DNA. Translation: AAA33943.1.
PIR	A33654.
RefSeq	NP_001238439.1. NM_001251510.1.
UniGene	Gma.33146.
3D structure databases
ProteinModelPortal	P32110.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA07G16810.1; GLYMA07G16810.1; GLYMA07G16810.
GeneID	100527851.
KEGG	gmx:100527851.
Phylogenomic databases
KO	K00799.
Enzyme and pathway databases
SABIO-RK	P32110.
Gene expression databases
Genevestigator	P32110.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTX6_SOYBN

Accession

Primary (citable) accession number: P32110

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program