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Protein

Growth hormone-releasing hormone receptor

Gene

Ghrhr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.

GO - Molecular functioni

  1. growth factor binding Source: MGI
  2. growth hormone-releasing hormone receptor activity Source: MGI
  3. peptide hormone binding Source: BHF-UCL

GO - Biological processi

  1. activation of adenylate cyclase activity Source: Ensembl
  2. adenohypophysis development Source: MGI
  3. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  4. cAMP-mediated signaling Source: MGI
  5. cell maturation Source: MGI
  6. cellular response to insulin stimulus Source: MGI
  7. determination of adult lifespan Source: MGI
  8. growth hormone secretion Source: MGI
  9. hormone metabolic process Source: MGI
  10. lactation Source: MGI
  11. mammary gland development Source: MGI
  12. positive regulation of cAMP biosynthetic process Source: MGI
  13. positive regulation of cell proliferation Source: BHF-UCL
  14. positive regulation of growth hormone secretion Source: MGI
  15. positive regulation of hormone secretion Source: MGI
  16. positive regulation of multicellular organism growth Source: MGI
  17. regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  18. regulation of intracellular steroid hormone receptor signaling pathway Source: MGI
  19. regulation of protein metabolic process Source: MGI
  20. response to estrogen Source: MGI
  21. response to glucocorticoid Source: MGI
  22. response to insulin Source: MGI
  23. somatotropin secreting cell development Source: MGI
  24. water homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_231304. Glucagon-type ligand receptors.
REACT_242871. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth hormone-releasing hormone receptor
Short name:
GHRH receptor
Alternative name(s):
Growth hormone-releasing factor receptor
Short name:
GRF receptor
Short name:
GRFR
Gene namesi
Name:Ghrhr
Synonyms:Grfr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:95710. Ghrhr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 132110ExtracellularSequence AnalysisAdd
BLAST
Transmembranei133 – 15220Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini153 – 16210CytoplasmicSequence Analysis
Transmembranei163 – 18119Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini182 – 20423ExtracellularSequence AnalysisAdd
BLAST
Transmembranei205 – 22723Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini228 – 24013CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei241 – 26222Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini263 – 28018ExtracellularSequence AnalysisAdd
BLAST
Transmembranei281 – 30424Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini305 – 32925CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei330 – 34819Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini349 – 36113ExtracellularSequence AnalysisAdd
BLAST
Transmembranei362 – 38120Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini382 – 42342CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. cytoplasm Source: MGI
  3. integral component of membrane Source: MGI
  4. nuclear inner membrane Source: MGI
  5. nuclear matrix Source: MGI
  6. nuclear outer membrane Source: MGI
  7. plasma membrane Source: MGI
  8. secretory granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Little (lit) mice exhibits anterior pituitary hypoplasia.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 423401Growth hormone-releasing hormone receptorPRO_0000012829Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 64By similarity
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi55 ↔ 96By similarity
Disulfide bondi78 ↔ 112By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP32082.
PRIDEiP32082.

Expressioni

Tissue specificityi

Pituitary gland.

Gene expression databases

BgeeiP32082.
CleanExiMM_GHRHR.
GenevestigatoriP32082.

Interactioni

Protein-protein interaction databases

BioGridi199917. 1 interaction.
STRINGi10090.ENSMUSP00000068120.

Structurei

3D structure databases

ProteinModelPortaliP32082.
SMRiP32082. Positions 34-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249002.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiP32082.
KOiK04584.
OMAiIHYIIFN.
OrthoDBiEOG7TF78W.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003288. GPCR_2_GHRH_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01352. GHRHRECEPTOR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32082-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGLMWATRI LCLLSLCGVT LGHLHLECDF ITQLRDDELA CLQAAEGTNN
60 70 80 90 100
TSLGCPGTWD GLLCWPPTGS GQWVSLPCPE FFSHFGSDTG FVKRDCTITG
110 120 130 140 150
WSNPFPPYPV ACPVPLELLT KEKSYFSTVK IIYTTGHSIS IVALCVAIAI
160 170 180 190 200
LVALRRLHCP RNYIHTQLFA TFILKASAVF LKDAAIFQGD STDHCSMSTV
210 220 230 240 250
LCKVSVAISH LATMTNFSWL LAEAVYLSCL LASTSPRSKP AFWWLVLAGW
260 270 280 290 300
GLPVLCTGTW VGCKLAFEDT ECWDLDNSSP CWWIIKGPIV LSVGVNFGLF
310 320 330 340 350
LNIICILLRK LEPAQGGLHT RAQYWRLSKS TLLLIPLFGI HYIIFNFLPD
360 370 380 390 400
SAGLDIRVPL ELGLGSFQGF IVAVLYCFLN QEVRTEISRK WYGHDPELLP
410 420
ARRTCTEWTT PPRSRLKVLT SEC
Length:423
Mass (Da):47,004
Last modified:July 27, 2011 - v2
Checksum:iA2BC658E719A8033
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2662LA → HS in L07379. (PubMed:1334535)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti60 – 601D → G in lit. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07379 mRNA. No translation available.
BC120748 mRNA. Translation: AAI20749.1.
BC120774 mRNA. Translation: AAI20775.1.
CCDSiCCDS20165.1.
PIRiS29753.
RefSeqiNP_001003685.2. NM_001003685.3.
UniGeneiMm.89928.

Genome annotation databases

EnsembliENSMUST00000063578; ENSMUSP00000068120; ENSMUSG00000004654.
GeneIDi14602.
KEGGimmu:14602.
UCSCiuc009cas.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07379 mRNA. No translation available.
BC120748 mRNA. Translation: AAI20749.1.
BC120774 mRNA. Translation: AAI20775.1.
CCDSiCCDS20165.1.
PIRiS29753.
RefSeqiNP_001003685.2. NM_001003685.3.
UniGeneiMm.89928.

3D structure databases

ProteinModelPortaliP32082.
SMRiP32082. Positions 34-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199917. 1 interaction.
STRINGi10090.ENSMUSP00000068120.

Chemistry

GuidetoPHARMACOLOGYi247.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP32082.
PRIDEiP32082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063578; ENSMUSP00000068120; ENSMUSG00000004654.
GeneIDi14602.
KEGGimmu:14602.
UCSCiuc009cas.2. mouse.

Organism-specific databases

CTDi2692.
MGIiMGI:95710. Ghrhr.

Phylogenomic databases

eggNOGiNOG249002.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiP32082.
KOiK04584.
OMAiIHYIIFN.
OrthoDBiEOG7TF78W.
TreeFamiTF315710.

Enzyme and pathway databases

ReactomeiREACT_231304. Glucagon-type ligand receptors.
REACT_242871. G alpha (s) signalling events.

Miscellaneous databases

NextBioi286380.
PROiP32082.
SOURCEiSearch...

Gene expression databases

BgeeiP32082.
CleanExiMM_GHRHR.
GenevestigatoriP32082.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003288. GPCR_2_GHRH_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01352. GHRHRECEPTOR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth."
    Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G.
    Nature 360:765-768(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Molecular basis of the little mouse phenotype and implications for cell type-specific growth."
    Lin S.-C., Lin C.R., Gukovsky I., Lusis A.J., Sawchenko P.E., Rosenfeld M.G.
    Nature 364:208-213(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT LIT GLY-60.

Entry informationi

Entry nameiGHRHR_MOUSE
AccessioniPrimary (citable) accession number: P32082
Secondary accession number(s): Q0VB62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.