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P32082 (GHRHR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Growth hormone-releasing hormone receptor

Short name=GHRH receptor
Alternative name(s):
Growth hormone-releasing factor receptor
Short name=GRF receptor
Short name=GRFR
Gene names
Name:Ghrhr
Synonyms:Grfr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Pituitary gland.

Involvement in disease

Little (lit) mice exhibits anterior pituitary hypoplasia.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DiseaseDisease mutation
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of adenylate cyclase activity

Inferred from electronic annotation. Source: Ensembl

adenohypophysis development

Inferred from mutant phenotype PubMed 1270792PubMed 1329576PubMed 1504465. Source: MGI

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 10537133PubMed 3008329. Source: MGI

cAMP-mediated signaling

Inferred from electronic annotation. Source: Ensembl

cell maturation

Inferred from mutant phenotype PubMed 2980124. Source: MGI

cellular response to insulin stimulus

Inferred from direct assay PubMed 16513828. Source: MGI

determination of adult lifespan

Inferred from mutant phenotype PubMed 11371619. Source: MGI

growth hormone secretion

Inferred from mutant phenotype PubMed 6303755PubMed 978118. Source: MGI

hormone metabolic process

Inferred from mutant phenotype PubMed 1270792. Source: MGI

lactation

Inferred from mutant phenotype PubMed 1270792PubMed 2980124. Source: MGI

mammary gland development

Inferred from mutant phenotype PubMed 15059953. Source: MGI

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 11773624. Source: BHF-UCL

positive regulation of growth hormone secretion

Inferred from mutant phenotype PubMed 3008329. Source: MGI

positive regulation of hormone secretion

Inferred from mutant phenotype PubMed 7679139. Source: MGI

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 10689811PubMed 1270792PubMed 15690087PubMed 1778592PubMed 7679139. Source: MGI

regulation of insulin-like growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 7390396PubMed 7679139. Source: MGI

regulation of intracellular steroid hormone receptor signaling pathway

Inferred from mutant phenotype PubMed 10459853. Source: MGI

regulation of protein metabolic process

Inferred from genetic interaction PubMed 7510770. Source: MGI

response to estrogen

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

somatotropin secreting cell development

Inferred from mutant phenotype PubMed 6116352PubMed 6194978. Source: MGI

water homeostasis

Inferred from genetic interaction PubMed 7510770. Source: MGI

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from direct assay PubMed 10537133. Source: MGI

nuclear inner membrane

Inferred from electronic annotation. Source: Ensembl

nuclear matrix

Inferred from electronic annotation. Source: Ensembl

nuclear outer membrane

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 10537133. Source: MGI

secretory granule

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiongrowth hormone-releasing hormone receptor activity

Inferred from mutant phenotype PubMed 10537133. Source: MGI

peptide hormone binding

Inferred from physical interaction PubMed 11773624. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 10537133. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 423401Growth hormone-releasing hormone receptor
PRO_0000012829

Regions

Topological domain23 – 132110Extracellular Potential
Transmembrane133 – 15220Helical; Name=1; Potential
Topological domain153 – 16210Cytoplasmic Potential
Transmembrane163 – 18119Helical; Name=2; Potential
Topological domain182 – 20423Extracellular Potential
Transmembrane205 – 22723Helical; Name=3; Potential
Topological domain228 – 24013Cytoplasmic Potential
Transmembrane241 – 26222Helical; Name=4; Potential
Topological domain263 – 28018Extracellular Potential
Transmembrane281 – 30424Helical; Name=5; Potential
Topological domain305 – 32925Cytoplasmic Potential
Transmembrane330 – 34819Helical; Name=6; Potential
Topological domain349 – 36113Extracellular Potential
Transmembrane362 – 38120Helical; Name=7; Potential
Topological domain382 – 42342Cytoplasmic Potential

Amino acid modifications

Glycosylation491N-linked (GlcNAc...) Potential
Glycosylation501N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 64 By similarity
Disulfide bond55 ↔ 96 By similarity
Disulfide bond78 ↔ 112 By similarity

Natural variations

Natural variant601D → G in lit. Ref.3

Experimental info

Sequence conflict265 – 2662LA → HS in L07379. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32082 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: A2BC658E719A8033

FASTA42347,004
        10         20         30         40         50         60 
MDGLMWATRI LCLLSLCGVT LGHLHLECDF ITQLRDDELA CLQAAEGTNN TSLGCPGTWD 

        70         80         90        100        110        120 
GLLCWPPTGS GQWVSLPCPE FFSHFGSDTG FVKRDCTITG WSNPFPPYPV ACPVPLELLT 

       130        140        150        160        170        180 
KEKSYFSTVK IIYTTGHSIS IVALCVAIAI LVALRRLHCP RNYIHTQLFA TFILKASAVF 

       190        200        210        220        230        240 
LKDAAIFQGD STDHCSMSTV LCKVSVAISH LATMTNFSWL LAEAVYLSCL LASTSPRSKP 

       250        260        270        280        290        300 
AFWWLVLAGW GLPVLCTGTW VGCKLAFEDT ECWDLDNSSP CWWIIKGPIV LSVGVNFGLF 

       310        320        330        340        350        360 
LNIICILLRK LEPAQGGLHT RAQYWRLSKS TLLLIPLFGI HYIIFNFLPD SAGLDIRVPL 

       370        380        390        400        410        420 
ELGLGSFQGF IVAVLYCFLN QEVRTEISRK WYGHDPELLP ARRTCTEWTT PPRSRLKVLT 


SEC 

« Hide

References

« Hide 'large scale' references
[1]"Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth."
Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G.
Nature 360:765-768(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Molecular basis of the little mouse phenotype and implications for cell type-specific growth."
Lin S.-C., Lin C.R., Gukovsky I., Lusis A.J., Sawchenko P.E., Rosenfeld M.G.
Nature 364:208-213(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT LIT GLY-60.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07379 mRNA. No translation available.
BC120748 mRNA. Translation: AAI20749.1.
BC120774 mRNA. Translation: AAI20775.1.
CCDSCCDS20165.1.
PIRS29753.
RefSeqNP_001003685.2. NM_001003685.3.
UniGeneMm.89928.

3D structure databases

ProteinModelPortalP32082.
SMRP32082. Positions 34-386.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199917. 1 interaction.
STRING10090.ENSMUSP00000068120.

Chemistry

GuidetoPHARMACOLOGY247.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbP32082.
PRIDEP32082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063578; ENSMUSP00000068120; ENSMUSG00000004654.
GeneID14602.
KEGGmmu:14602.
UCSCuc009cas.2. mouse.

Organism-specific databases

CTD2692.
MGIMGI:95710. Ghrhr.

Phylogenomic databases

eggNOGNOG249002.
GeneTreeENSGT00740000115231.
HOGENOMHOG000008249.
HOVERGENHBG008318.
InParanoidQ0VB62.
KOK04584.
OMAIHYIIFN.
OrthoDBEOG7TF78W.
TreeFamTF315710.

Gene expression databases

BgeeP32082.
CleanExMM_GHRHR.
GenevestigatorP32082.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003288. GPCR_2_GHRH_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR01352. GHRHRECEPTOR.
PR00249. GPCRSECRETIN.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286380.
PROP32082.
SOURCESearch...

Entry information

Entry nameGHRHR_MOUSE
AccessionPrimary (citable) accession number: P32082
Secondary accession number(s): Q0VB62
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries