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Protein

Cold shock protein CspB

Gene

cspB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU09100-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cold shock protein CspB
Alternative name(s):
Major cold shock protein
Gene namesi
Name:cspB
Synonyms:cspA
Ordered Locus Names:BSU09100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasmnucleoid 1 Publication

  • Note: Shows transcription-dependent localization at subcellular sites surrounding the nucleoid.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

A double cshB-cspB disruption mutant cannot be made.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6767Cold shock protein CspBPRO_0000100295Add
BLAST

Proteomic databases

PaxDbiP32081.

Expressioni

Inductioni

In response to low temperature.

Interactioni

Subunit structurei

Homodimer. Interacts with RNA helicase CshB when cells are transcriptionally active.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005033.

Structurei

Secondary structure

1
67
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109Combined sources
Turni11 – 144Combined sources
Beta strandi15 – 195Combined sources
Beta strandi21 – 233Combined sources
Beta strandi26 – 294Combined sources
Helixi30 – 323Combined sources
Beta strandi35 – 384Combined sources
Beta strandi46 – 549Combined sources
Beta strandi57 – 659Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CSPX-ray2.45A1-67[»]
1CSQX-ray2.70A1-67[»]
1NMFNMR-A1-67[»]
1NMGNMR-A1-67[»]
2ES2X-ray1.78A1-67[»]
2F52NMR-A1-67[»]
2I5LX-ray2.55X1-67[»]
2I5MX-ray2.30X1-67[»]
3PF4X-ray1.38A/B1-67[»]
3PF5X-ray1.68A/B1-67[»]
ProteinModelPortaliP32081.
SMRiP32081. Positions 1-67.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32081.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6360CSDAdd
BLAST

Sequence similaritiesi

Contains 1 CSD (cold-shock) domain.Curated

Phylogenomic databases

eggNOGiENOG4105VEQ. Bacteria.
COG1278. LUCA.
HOGENOMiHOG000070674.
InParanoidiP32081.
KOiK03704.
OMAiFIQVENG.
OrthoDBiEOG618R0J.
PhylomeDBiP32081.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00313. CSD. 1 hit.
[Graphical view]
PIRSFiPIRSF002599. Cold_shock_A. 1 hit.
PRINTSiPR00050. COLDSHOCK.
SMARTiSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00352. COLD_SHOCK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEGKVKWFN SEKGFGFIEV EGQDDVFVHF SAIQGEGFKT LEEGQAVSFE
60
IVEGNRGPQA ANVTKEA
Length:67
Mass (Da):7,365
Last modified:October 1, 1993 - v1
Checksum:i1E7340FDB19E5BDC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171F → L in strain: Globigii.
Natural varianti44 – 441G → S in strain: Globigii.
Natural varianti46 – 461A → S.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59715 Genomic DNA. Translation: CAA42235.1.
U58859 Genomic DNA. Translation: AAB01346.1.
X96983 Genomic DNA. Translation: CAA65693.1.
AL009126 Genomic DNA. Translation: CAB12738.1.
U60029 Genomic DNA. Translation: AAC80233.1.
U60030 Genomic DNA. Translation: AAC80234.1.
X73463 Genomic DNA. Translation: CAA51842.1.
PIRiA45723.
RefSeqiNP_388791.1. NC_000964.3.
WP_003224086.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB12738; CAB12738; BSU09100.
GeneIDi11238806.
936224.
KEGGibsu:BSU09100.
PATRICi18973518. VBIBacSub10457_0953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59715 Genomic DNA. Translation: CAA42235.1.
U58859 Genomic DNA. Translation: AAB01346.1.
X96983 Genomic DNA. Translation: CAA65693.1.
AL009126 Genomic DNA. Translation: CAB12738.1.
U60029 Genomic DNA. Translation: AAC80233.1.
U60030 Genomic DNA. Translation: AAC80234.1.
X73463 Genomic DNA. Translation: CAA51842.1.
PIRiA45723.
RefSeqiNP_388791.1. NC_000964.3.
WP_003224086.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CSPX-ray2.45A1-67[»]
1CSQX-ray2.70A1-67[»]
1NMFNMR-A1-67[»]
1NMGNMR-A1-67[»]
2ES2X-ray1.78A1-67[»]
2F52NMR-A1-67[»]
2I5LX-ray2.55X1-67[»]
2I5MX-ray2.30X1-67[»]
3PF4X-ray1.38A/B1-67[»]
3PF5X-ray1.68A/B1-67[»]
ProteinModelPortaliP32081.
SMRiP32081. Positions 1-67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005033.

Proteomic databases

PaxDbiP32081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12738; CAB12738; BSU09100.
GeneIDi11238806.
936224.
KEGGibsu:BSU09100.
PATRICi18973518. VBIBacSub10457_0953.

Phylogenomic databases

eggNOGiENOG4105VEQ. Bacteria.
COG1278. LUCA.
HOGENOMiHOG000070674.
InParanoidiP32081.
KOiK03704.
OMAiFIQVENG.
OrthoDBiEOG618R0J.
PhylomeDBiP32081.

Enzyme and pathway databases

BioCyciBSUB:BSU09100-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32081.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00313. CSD. 1 hit.
[Graphical view]
PIRSFiPIRSF002599. Cold_shock_A. 1 hit.
PRINTSiPR00050. COLDSHOCK.
SMARTiSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00352. COLD_SHOCK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures."
    Willimsky G., Bang H., Fisher G., Marahiel M.A.
    J. Bacteriol. 174:6326-6335(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Wendrich T.M., Marahiel M.A.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  3. "A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the Bacillus subtilis chromosome."
    Noback M.A., Terpstra P., Holsappel S., Venema G., Bron S.
    Microbiology 142:3021-3026(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "Mapping of the Bacillus subtilis cspB gene and cloning of its homologs in thermophilic, mesophilic and psychrotrophic bacilli."
    Schroeder K., Zuber P., Willimsky G., Wagner B., Marahiel M.A.
    Gene 136:277-280(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-62.
    Strain: Globigii.
  6. "Detection and speciation of bacteria through PCR using universal major cold-shock protein primer oligomers."
    Francis K.P., Stewart G.S.A.B.
    J. Ind. Microbiol. Biotechnol. 19:286-293(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-56.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  7. "Cold shock stress-induced proteins in Bacillus subtilis."
    Graumann P., Schroeder K., Schmid R., Marahiel M.A.
    J. Bacteriol. 178:4611-4619(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-31.
    Strain: 168 / JH642.
  8. "The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides."
    Graumann P., Marahiel M.A.
    FEBS Lett. 338:157-160(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis."
    Hunger K., Beckering C.L., Wiegeshoff F., Graumann P.L., Marahiel M.A.
    J. Bacteriol. 188:240-248(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CSHB, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: 168 / JH642.
  10. "Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein."
    Schindelin H., Marahiel M.A., Heinemann U.
    Nature 364:164-168(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  11. "Structure in solution of the major cold-shock protein from Bacillus subtilis."
    Schnuchel A., Wiltscheck R., Czisch M., Herrier M., Willimsky G., Graumann P., Marahiel M.A., Holak T.A.
    Nature 364:169-171(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiCSPB_BACSU
AccessioniPrimary (citable) accession number: P32081
Secondary accession number(s): P41017, Q45690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 17, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.