P32074 (COPG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coatomer subunit gamma

Alternative name(s):
Gamma-coat protein
Short name=Gamma-COP

Gene names

Name:	SEC21
Ordered Locus Names:	YNL287W
ORF Names:	N0543

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	935 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Ref.1 Ref.4
Subunit structure	Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts (via C-terminus) with GEA1 (via N-terminal region) and KEI1 (via C-terminal region). Ref.1 Ref.10 Ref.11
Subcellular location	Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endosome. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity. Ref.1 Ref.5 Ref.7
Miscellaneous	Present with 77900 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the COPG family. Contains 5 HEAT repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Endosome Golgi apparatus Membrane
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.1. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde vesicle-mediated transport, Golgi to ER Inferred from mutant phenotype Ref.4. Source: SGD
Cellular component	COPI vesicle coat Inferred from direct assay Ref.1. Source: SGD endosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GLO3	P38682	3	EBI-4891,EBI-7679
SEC27	P41811	4	EBI-4891,EBI-4898

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 935	935	Coatomer subunit gamma		PRO_0000193861
Regions
	Repeat	258 – 296	39	HEAT 1
	Repeat	337 – 372	36	HEAT 2
	Repeat	373 – 410	38	HEAT 3
	Repeat	412 – 449	38	HEAT 4
	Repeat	524 – 562	39	HEAT 5
Amino acid modifications
	Modified residue	504	1	Phosphoserine Ref.9
	Modified residue	581	1	Phosphoserine Ref.9
	Modified residue	637	1	Phosphothreonine Ref.8 Ref.9
	Modified residue	638	1	Phosphothreonine Ref.8 Ref.9
	Modified residue	653	1	Phosphoserine Ref.8 Ref.9
	Modified residue	695	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	353	1	D → N in AAA34598. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32074 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 99DC7D737D4EF761	FASTA	935	104,831
	10 20 30 40 50 60 MSAHTYKKFE NSTSGDLPDK MTIYQDCMNT FNESPVNSKR CRLLISRLLR LLAQGETFPQ 70 80 90 100 110 120 NEATALFFSI SKLFQHQNDP LRQAVYLAIK ELSGISEDVL MATSSIMKDV QNGSDLIKPD 130 140 150 160 170 180 AIRSLTYVLD ESTAFSAERL LKSAVVSRHP SISSAALCTS YHLLPISEVT IRRFTNETQE 190 200 210 220 230 240 AVLDLKQFPN QHGNSEYYPN STYISQYHAL GLLYQLKKTD KMALLKLVRH FSENNSMKNQ 250 260 270 280 290 300 LAKVELVKIV NDLIYRDPQL FSQFRPLLSD WLSNKFESVQ LETAKLITSF ATRNSRLVAP 310 320 330 340 350 360 ELYAAAISAL QSLLTVPRVS SRFAALRILN RISMVSPEKI VVCNPELESL INDSNRNIST 370 380 390 400 410 420 YAITTLLKTG TSKNISSLIS TITNFIHDVS DDFKIIIIDA VRTLSLNFPQ EWKSILNFLI 430 440 450 460 470 480 DVLKNSEGGF KFKNSIVEAL IDIVSFVPQS KELALENLCD FIEDCEFNEI LVRILHLLGK 490 500 510 520 530 540 EGPSAPNPSL YVRHIYNRVV LENSIIRSAA VVALSKFALT KNDPTLYESI ISLLKRIAND 550 560 570 580 590 600 KDDEVRDRAT IALEFIDSAR NKDDVIAQNL IESKYFYDIP SLESKLSSYI SSNTDSFATA 610 620 630 640 650 660 FDVNQVRKFT EDEMKAINLK RKQEQIFNQK SETTLDTTPE AESVPEKRAD ANSFAGPNLD 670 680 690 700 710 720 DHQEDLLATK YADELLSIEQ IKPFGQLVNS SRAISLTEPE AEFVVRGVKH LFKDNVVLQF 730 740 750 760 770 780 NITNTLTDIA LDNVSVVCTP EISDEAELEE LFTLQVDRLL PSEEAACYVA FKKLDEIVME 790 800 810 820 830 840 GFLNNLTFTT KEINPDTNEP FDGDEGFQDE YEIDSIFLNA GDYVKSSFTG NFSATFDELP 850 860 870 880 890 900 CEEVAVFNIQ EDLSIQEVVD KIILNSSCLP VESTQFAPSD SNSHTLKLFG KSALTGSKVA 910 920 930 LQIKMIKSSK GLALKVHGKG EDSLLCSDLV NGLMQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer." Hosobuchi M.M., Kreis T., Schekman R. Nature 360:603-605(1992) [PubMed: 1461285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum." Letourneur F., Gaynor E.C., Hennecke S., Demolliere C., Durden R., Emr S.D., Riezman H., Cosson P. Cell 79:1199-1207(1994) [PubMed: 8001155] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast." Gabriely G., Kama R., Gerst J.E. Mol. Cell. Biol. 27:526-540(2007) [PubMed: 17101773] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-637; THR-638 AND SER-653, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-504; SER-581; THR-637; THR-638; SER-653 AND SER-695, MASS SPECTROMETRY.
[10]	"A COPI coat subunit interacts directly with an early-Golgi localized Arf exchange factor." Deng Y., Golinelli-Cohen M.P., Smirnova E., Jackson C.L. EMBO Rep. 10:58-64(2009) [PubMed: 19039328] [Abstract] Cited for: INTERACTION WITH GEA1.
[11]	"Kei1: a novel subunit of inositolphosphorylceramide synthase, essential for its enzyme activity and Golgi localization." Sato K., Noda Y., Yoda K. Mol. Biol. Cell 20:4444-4457(2009) [PubMed: 19726565] [Abstract] Cited for: INTERACTION WITH KEI1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59708 Genomic DNA. Translation: AAA34598.1. Z71563 Genomic DNA. Translation: CAA96204.1. BK006947 Genomic DNA. Translation: DAA10272.1.
PIR	S63261.
RefSeq	NP_014112.1. NM_001183125.1.
3D structure databases
ProteinModelPortal	P32074.
SMR	P32074. Positions 307-334, 474-514, 661-933.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1611N.
IntAct	P32074. 22 interactions.
MINT	MINT-399140.
STRING	P32074.
Proteomic databases
PeptideAtlas	P32074.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL287W; YNL287W; YNL287W.
GeneID	855429.
KEGG	sce:YNL287W.
NMPDR	fig|4932.3.peg.5174.
Organism-specific databases
CYGD	YNL287w.
SGD	S000005231. SEC21.
Phylogenomic databases
eggNOG	fuNOG05600.
GeneTree	EFGT00050000003092.
HOGENOM	HBG631201.
OMA	GTFRGGV.
OrthoDB	EOG4FFH8X.
Gene expression databases
ArrayExpress	P32074.
Genevestigator	P32074.
GermOnline	YNL287W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. IPR015873. Clathrin_a/coatomer_app_sub_C. IPR009028. Coatomer/calthrin_app_sub_C. IPR013041. Coatomer/clathrin_app_Ig-like. IPR017106. Coatomer_gsu. IPR014863. Coatomer_gsu_app. IPR013040. Coatomer_gsu_app_Ig-like-sub. [Graphical view]
Gene3D	G3DSA:3.30.310.30. AP2_A_adaptin_C. 1 hit. G3DSA:1.25.10.10. ARM-like. 1 hit. G3DSA:2.60.40.1480. Coatomer_gsu_app_Ig-like-sub. 1 hit.
PANTHER	PTHR10261. PTHR10261. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. PF08752. Gamma-COP. 1 hit. [Graphical view]
PIRSF	PIRSF037093. Coatomer_gamma_subunit. 1 hit.
SUPFAM	SSF55711. AP2_adap_app. 1 hit. SSF48371. ARM-type_fold. 1 hit. SSF49348. Clath_adapt. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979304.

Entry information

Entry name

COPG_YEAST

Accession

Primary (citable) accession number: P32074
Secondary accession number(s): D6W0Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program