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P32073 (GUAA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GMP synthase [glutamine-hydrolyzing]

EC=6.3.5.2
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene names
Name:guaA
Synonyms:gua2
ORF Names:DDB_G0281551
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathway

Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.

Subunit structure

Homodimer By similarity.

Developmental stage

Produced during growth but not during development.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718GMP synthase [glutamine-hydrolyzing]
PRO_0000140256

Regions

Domain43 – 247205Glutamine amidotransferase type-1
Domain248 – 457210GMPS ATP-PPase
Nucleotide binding275 – 2817ATP By similarity

Sites

Active site1281For GATase activity By similarity
Active site2211For GATase activity By similarity
Active site2231For GATase activity By similarity

Experimental info

Sequence conflict5351G → V in AAA33213. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32073 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: DCB5DCDE84793DEA

FASTA71879,562
        10         20         30         40         50         60 
MTITSPVIKT PPLNSEIRLE SNLTVESGDI EINEKDIVNA SEVIVILDAG SQYSKVIDRR 

        70         80         90        100        110        120 
VRELNVASEI HPLNIDLLEL IKIKSKSGST IKGIIISGGP ESVYGENAPK FDKSLFSEKL 

       130        140        150        160        170        180 
NLPIFGICYG MQLMNYIFGG KVESNSQRED GVHNIEILKD ENQQLVSKLF KNLNQTEQVL 

       190        200        210        220        230        240 
LTHGDSVTKI ADGFKIICKS DDGIVSGIEN ERLGYYGVQF HPEVDLTTNG KKMFSNFLID 

       250        260        270        280        290        300 
ICGCSANYTL DDREQQAITY IKSIVSNKKV LVLVSGGVDS TVCAALISKA IGPENVIALH 

       310        320        330        340        350        360 
IDNGFMRKDE SLNVEKALSV LGLHLIVVDA SQTFYNSTTT IKGHLTSSLK ETISPEERRK 

       370        380        390        400        410        420 
IIGDTFMRVA ENEVKKLGLQ PEDVYLAQGT LRPDLIESSS KTVSGVADVI KTHHNDTELV 

       430        440        450        460        470        480 
RILRDSGRVV EPLKDYHKDE VRELGKSLGL SDSLVWRQPF PGPGLAIRII CADEPYLVNY 

       490        500        510        520        530        540 
DFTNNVVQYL VTGEASSELE SEVKIKIDKQ LTEMKCKRQD KITIKPVLLP IQTVGVQGDG 

       550        560        570        580        590        600 
RTYSYLLGLY SSENSTIDQI PWSYIFNLAR TIPKICHNIN RVVFIFSQNA TKHTNIKVSN 

       610        620        630        640        650        660 
EPVKHITPTR LTPDVIKQLQ HADSIVSEQL YKYNLIKSLS QVPVVSLPID FGVTGNRSIA 

       670        680        690        700        710 
IRTFITNDFM TGVPAIPGKN ISFDCLQEIT NNILTSVNGI SKVLFDCTSK PPGTTEFL 

« Hide

References

« Hide 'large scale' references
[1]"Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase."
van Lookeren Campagne M.M., Franke J., Kessin R.H.
J. Biol. Chem. 266:16448-16452(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64282 mRNA. Translation: AAA33213.1.
AAFI02000042 Genomic DNA. Translation: EAL66537.1.
PIRA41164.

3D structure databases

ProteinModelPortalP32073.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0215334.

Protein family/group databases

MEROPSC26.962.

Proteomic databases

PRIDEP32073.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0215334; DDB0215334; DDB_G0281551.
KEGGddi:DDB_G0281551.

Organism-specific databases

dictyBaseDDB_G0281551. guaA.

Phylogenomic databases

eggNOGCOG0519.
KOK01951.
OMAINRVVYV.
PhylomeDBP32073.
ProtClustDBCLSZ2430524.

Enzyme and pathway databases

UniPathwayUPA00189; UER00296.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 2 hits.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00888. guaA_Nterm. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP32073.

Entry information

Entry nameGUAA_DICDI
AccessionPrimary (citable) accession number: P32073
Secondary accession number(s): Q54TQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase