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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128For GATase activityPROSITE-ProRule annotation1
Active sitei221For GATase activityPROSITE-ProRule annotation1
Active sitei223For GATase activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi275 – 281ATPPROSITE-ProRule annotation7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GMP synthase (glutamine-hydrolyzing) activity Source: dictyBase
  • GMP synthase activity Source: GO_Central
  • pyrophosphatase activity Source: InterPro

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • GMP biosynthetic process Source: dictyBase
  • GMP metabolic process Source: dictyBase

Keywordsi

Molecular functionLigase
Biological processGMP biosynthesis, Purine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00189; UER00296

Protein family/group databases

MEROPSiC26.962

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Synonyms:gua2
ORF Names:DDB_G0281551
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0281551 guaA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001402561 – 718GMP synthase [glutamine-hydrolyzing]Add BLAST718

Proteomic databases

PaxDbiP32073
PRIDEiP32073

Expressioni

Developmental stagei

Produced during growth but not during development.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0215334

Structurei

3D structure databases

ProteinModelPortaliP32073
SMRiP32073
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 247Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST205
Domaini248 – 457GMPS ATP-PPasePROSITE-ProRule annotationAdd BLAST210

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1622 Eukaryota
COG0518 LUCA
COG0519 LUCA
InParanoidiP32073
KOiK01951
OMAiDKWVGVK
PhylomeDBiP32073

Family and domain databases

CDDicd01742 GATase1_GMP_Synthase, 1 hit
cd01997 GMP_synthase_C, 1 hit
Gene3Di3.40.50.620, 1 hit
3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR001674 GMP_synth_C
IPR004739 GMP_synth_GATase
IPR025777 GMPS_ATP_PPase_dom
IPR022310 NAD/GMP_synthase
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00958 GMP_synt_C, 2 hits
PF02540 NAD_synthase, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00888 guaA_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS51553 GMPS_ATP_PPASE, 1 hit

Sequencei

Sequence statusi: Complete.

P32073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITSPVIKT PPLNSEIRLE SNLTVESGDI EINEKDIVNA SEVIVILDAG
60 70 80 90 100
SQYSKVIDRR VRELNVASEI HPLNIDLLEL IKIKSKSGST IKGIIISGGP
110 120 130 140 150
ESVYGENAPK FDKSLFSEKL NLPIFGICYG MQLMNYIFGG KVESNSQRED
160 170 180 190 200
GVHNIEILKD ENQQLVSKLF KNLNQTEQVL LTHGDSVTKI ADGFKIICKS
210 220 230 240 250
DDGIVSGIEN ERLGYYGVQF HPEVDLTTNG KKMFSNFLID ICGCSANYTL
260 270 280 290 300
DDREQQAITY IKSIVSNKKV LVLVSGGVDS TVCAALISKA IGPENVIALH
310 320 330 340 350
IDNGFMRKDE SLNVEKALSV LGLHLIVVDA SQTFYNSTTT IKGHLTSSLK
360 370 380 390 400
ETISPEERRK IIGDTFMRVA ENEVKKLGLQ PEDVYLAQGT LRPDLIESSS
410 420 430 440 450
KTVSGVADVI KTHHNDTELV RILRDSGRVV EPLKDYHKDE VRELGKSLGL
460 470 480 490 500
SDSLVWRQPF PGPGLAIRII CADEPYLVNY DFTNNVVQYL VTGEASSELE
510 520 530 540 550
SEVKIKIDKQ LTEMKCKRQD KITIKPVLLP IQTVGVQGDG RTYSYLLGLY
560 570 580 590 600
SSENSTIDQI PWSYIFNLAR TIPKICHNIN RVVFIFSQNA TKHTNIKVSN
610 620 630 640 650
EPVKHITPTR LTPDVIKQLQ HADSIVSEQL YKYNLIKSLS QVPVVSLPID
660 670 680 690 700
FGVTGNRSIA IRTFITNDFM TGVPAIPGKN ISFDCLQEIT NNILTSVNGI
710
SKVLFDCTSK PPGTTEFL
Length:718
Mass (Da):79,562
Last modified:December 4, 2007 - v2
Checksum:iDCB5DCDE84793DEA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti535G → V in AAA33213 (PubMed:1885577).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64282 mRNA Translation: AAA33213.1
AAFI02000042 Genomic DNA Translation: EAL66537.1
PIRiA41164
RefSeqiXP_640534.1, XM_635442.1

Genome annotation databases

EnsemblProtistsiEAL66537; EAL66537; DDB_G0281551
GeneIDi8623144
KEGGiddi:DDB_G0281551

Similar proteinsi

Entry informationi

Entry nameiGUAA_DICDI
AccessioniPrimary (citable) accession number: P32073
Secondary accession number(s): Q54TQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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