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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei128 – 1281For GATase activityPROSITE-ProRule annotation
Active sitei221 – 2211For GATase activityPROSITE-ProRule annotation
Active sitei223 – 2231For GATase activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi275 – 2817ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GMP synthase (glutamine-hydrolyzing) activity Source: dictyBase
  • GMP synthase activity Source: GO_Central
  • pyrophosphatase activity Source: InterPro

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • GMP biosynthetic process Source: dictyBase
  • GMP metabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.962.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Synonyms:gua2
ORF Names:DDB_G0281551
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0281551. guaA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718GMP synthase [glutamine-hydrolyzing]PRO_0000140256Add
BLAST

Proteomic databases

PaxDbiP32073.
PRIDEiP32073.

Expressioni

Developmental stagei

Produced during growth but not during development.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0215334.

Structurei

3D structure databases

ProteinModelPortaliP32073.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 247205Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST
Domaini248 – 457210GMPS ATP-PPasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation
Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1622. Eukaryota.
COG0518. LUCA.
COG0519. LUCA.
InParanoidiP32073.
KOiK01951.
OMAiNMTTNPE.
PhylomeDBiP32073.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 2 hits.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITSPVIKT PPLNSEIRLE SNLTVESGDI EINEKDIVNA SEVIVILDAG
60 70 80 90 100
SQYSKVIDRR VRELNVASEI HPLNIDLLEL IKIKSKSGST IKGIIISGGP
110 120 130 140 150
ESVYGENAPK FDKSLFSEKL NLPIFGICYG MQLMNYIFGG KVESNSQRED
160 170 180 190 200
GVHNIEILKD ENQQLVSKLF KNLNQTEQVL LTHGDSVTKI ADGFKIICKS
210 220 230 240 250
DDGIVSGIEN ERLGYYGVQF HPEVDLTTNG KKMFSNFLID ICGCSANYTL
260 270 280 290 300
DDREQQAITY IKSIVSNKKV LVLVSGGVDS TVCAALISKA IGPENVIALH
310 320 330 340 350
IDNGFMRKDE SLNVEKALSV LGLHLIVVDA SQTFYNSTTT IKGHLTSSLK
360 370 380 390 400
ETISPEERRK IIGDTFMRVA ENEVKKLGLQ PEDVYLAQGT LRPDLIESSS
410 420 430 440 450
KTVSGVADVI KTHHNDTELV RILRDSGRVV EPLKDYHKDE VRELGKSLGL
460 470 480 490 500
SDSLVWRQPF PGPGLAIRII CADEPYLVNY DFTNNVVQYL VTGEASSELE
510 520 530 540 550
SEVKIKIDKQ LTEMKCKRQD KITIKPVLLP IQTVGVQGDG RTYSYLLGLY
560 570 580 590 600
SSENSTIDQI PWSYIFNLAR TIPKICHNIN RVVFIFSQNA TKHTNIKVSN
610 620 630 640 650
EPVKHITPTR LTPDVIKQLQ HADSIVSEQL YKYNLIKSLS QVPVVSLPID
660 670 680 690 700
FGVTGNRSIA IRTFITNDFM TGVPAIPGKN ISFDCLQEIT NNILTSVNGI
710
SKVLFDCTSK PPGTTEFL
Length:718
Mass (Da):79,562
Last modified:December 4, 2007 - v2
Checksum:iDCB5DCDE84793DEA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti535 – 5351G → V in AAA33213 (PubMed:1885577).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64282 mRNA. Translation: AAA33213.1.
AAFI02000042 Genomic DNA. Translation: EAL66537.1.
PIRiA41164.
RefSeqiXP_640534.1. XM_635442.1.

Genome annotation databases

EnsemblProtistsiDDB0215334; DDB0215334; DDB_G0281551.
GeneIDi8623144.
KEGGiddi:DDB_G0281551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64282 mRNA. Translation: AAA33213.1.
AAFI02000042 Genomic DNA. Translation: EAL66537.1.
PIRiA41164.
RefSeqiXP_640534.1. XM_635442.1.

3D structure databases

ProteinModelPortaliP32073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0215334.

Protein family/group databases

MEROPSiC26.962.

Proteomic databases

PaxDbiP32073.
PRIDEiP32073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0215334; DDB0215334; DDB_G0281551.
GeneIDi8623144.
KEGGiddi:DDB_G0281551.

Organism-specific databases

dictyBaseiDDB_G0281551. guaA.

Phylogenomic databases

eggNOGiKOG1622. Eukaryota.
COG0518. LUCA.
COG0519. LUCA.
InParanoidiP32073.
KOiK01951.
OMAiNMTTNPE.
PhylomeDBiP32073.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
ReactomeiR-DDI-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiP32073.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 2 hits.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_DICDI
AccessioniPrimary (citable) accession number: P32073
Secondary accession number(s): Q54TQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.