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P32073

- GUAA_DICDI

UniProt

P32073 - GUAA_DICDI

Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei128 – 1281For GATase activityPROSITE-ProRule annotation
    Active sitei221 – 2211For GATase activityPROSITE-ProRule annotation
    Active sitei223 – 2231For GATase activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi275 – 2817ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: dictyBase
    2. GMP synthase (glutamine-hydrolyzing) activity Source: dictyBase
    3. pyrophosphatase activity Source: InterPro

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. GMP biosynthetic process Source: dictyBase
    3. GMP metabolic process Source: dictyBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00189; UER00296.

    Protein family/group databases

    MEROPSiC26.962.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
    Alternative name(s):
    GMP synthetase
    Glutamine amidotransferase
    Gene namesi
    Name:guaA
    Synonyms:gua2
    ORF Names:DDB_G0281551
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0281551. guaA.

    Subcellular locationi

    GO - Cellular componenti

    1. phagocytic vesicle Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 718718GMP synthase [glutamine-hydrolyzing]PRO_0000140256Add
    BLAST

    Proteomic databases

    PRIDEiP32073.

    Expressioni

    Developmental stagei

    Produced during growth but not during development.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0215334.

    Structurei

    3D structure databases

    ProteinModelPortaliP32073.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 247205Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini248 – 457210GMPS ATP-PPasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation
    Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0519.
    KOiK01951.
    OMAiGTVHKKN.
    PhylomeDBiP32073.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR001674. GMP_synth_C.
    IPR004739. GMP_synth_N.
    IPR025777. GMPS_ATP_PPase_dom.
    IPR022310. NAD/GMP_synthase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    PF00958. GMP_synt_C. 2 hits.
    PF02540. NAD_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    PS51553. GMPS_ATP_PPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32073-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTITSPVIKT PPLNSEIRLE SNLTVESGDI EINEKDIVNA SEVIVILDAG    50
    SQYSKVIDRR VRELNVASEI HPLNIDLLEL IKIKSKSGST IKGIIISGGP 100
    ESVYGENAPK FDKSLFSEKL NLPIFGICYG MQLMNYIFGG KVESNSQRED 150
    GVHNIEILKD ENQQLVSKLF KNLNQTEQVL LTHGDSVTKI ADGFKIICKS 200
    DDGIVSGIEN ERLGYYGVQF HPEVDLTTNG KKMFSNFLID ICGCSANYTL 250
    DDREQQAITY IKSIVSNKKV LVLVSGGVDS TVCAALISKA IGPENVIALH 300
    IDNGFMRKDE SLNVEKALSV LGLHLIVVDA SQTFYNSTTT IKGHLTSSLK 350
    ETISPEERRK IIGDTFMRVA ENEVKKLGLQ PEDVYLAQGT LRPDLIESSS 400
    KTVSGVADVI KTHHNDTELV RILRDSGRVV EPLKDYHKDE VRELGKSLGL 450
    SDSLVWRQPF PGPGLAIRII CADEPYLVNY DFTNNVVQYL VTGEASSELE 500
    SEVKIKIDKQ LTEMKCKRQD KITIKPVLLP IQTVGVQGDG RTYSYLLGLY 550
    SSENSTIDQI PWSYIFNLAR TIPKICHNIN RVVFIFSQNA TKHTNIKVSN 600
    EPVKHITPTR LTPDVIKQLQ HADSIVSEQL YKYNLIKSLS QVPVVSLPID 650
    FGVTGNRSIA IRTFITNDFM TGVPAIPGKN ISFDCLQEIT NNILTSVNGI 700
    SKVLFDCTSK PPGTTEFL 718
    Length:718
    Mass (Da):79,562
    Last modified:December 4, 2007 - v2
    Checksum:iDCB5DCDE84793DEA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti535 – 5351G → V in AAA33213. (PubMed:1885577)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64282 mRNA. Translation: AAA33213.1.
    AAFI02000042 Genomic DNA. Translation: EAL66537.1.
    PIRiA41164.
    RefSeqiXP_640534.1. XM_635442.1.

    Genome annotation databases

    EnsemblProtistsiDDB0215334; DDB0215334; DDB_G0281551.
    GeneIDi8623144.
    KEGGiddi:DDB_G0281551.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64282 mRNA. Translation: AAA33213.1 .
    AAFI02000042 Genomic DNA. Translation: EAL66537.1 .
    PIRi A41164.
    RefSeqi XP_640534.1. XM_635442.1.

    3D structure databases

    ProteinModelPortali P32073.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0215334.

    Protein family/group databases

    MEROPSi C26.962.

    Proteomic databases

    PRIDEi P32073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0215334 ; DDB0215334 ; DDB_G0281551 .
    GeneIDi 8623144.
    KEGGi ddi:DDB_G0281551.

    Organism-specific databases

    dictyBasei DDB_G0281551. guaA.

    Phylogenomic databases

    eggNOGi COG0519.
    KOi K01951.
    OMAi GTVHKKN.
    PhylomeDBi P32073.

    Enzyme and pathway databases

    UniPathwayi UPA00189 ; UER00296 .

    Miscellaneous databases

    PROi P32073.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR001674. GMP_synth_C.
    IPR004739. GMP_synth_N.
    IPR025777. GMPS_ATP_PPase_dom.
    IPR022310. NAD/GMP_synthase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    PF00958. GMP_synt_C. 2 hits.
    PF02540. NAD_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00888. guaA_Nterm. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    PS51553. GMPS_ATP_PPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase."
      van Lookeren Campagne M.M., Franke J., Kessin R.H.
      J. Biol. Chem. 266:16448-16452(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiGUAA_DICDI
    AccessioniPrimary (citable) accession number: P32073
    Secondary accession number(s): Q54TQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3