P32069 (TRPX_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component I-2, chloroplastic
EC=4.1.3.27

Gene names

Name:	ASA2
Ordered Locus Names:	At2g29690
ORF Names:	T27A16.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Subcellular location	Plastid › chloroplast Probable.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from genetic interaction Ref.1. Source: TAIR tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	anthranilate synthase complex Non-traceable author statement Ref.1. Source: TAIR chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR
Molecular_function	anthranilate synthase activity Inferred from genetic interaction Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 87	87	Chloroplast Potential
	Chain	88 – 621	534	Anthranilate synthase component I-2, chloroplastic		PRO_0000035790
Experimental info
	Sequence conflict	85	1	F → L in BAD95154. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P32069 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: D4039FE58A420967	FASTA	621	69,816
	10 20 30 40 50 60 MSAVSISAVK SDFFTVEAIA VTHHRTPHPP HFPSLRFPLS LKSPPATSLN LVAGSKLLHF 70 80 90 100 110 120 SRRLPSIKCS YTPSLDLSEE QFTKFKKASE KGNLVPLFRC VFSDHLTPIL AYRCLVKEDD 130 140 150 160 170 180 RDAPSFLFES VEPGSQSSNI GRYSVVGAQP TIEIVAKGNV VTVMDHGASL RTEEEVDDPM 190 200 210 220 230 240 MVPQKIMEEW NPQGIDELPE AFCGGWVGYF SYDTVRYVEK KKLPFSNAPE DDRSLPDVNL 250 260 270 280 290 300 GLYDDVIVFD HVEKKAYVIH WVRIDKDRSV EENFREGMNR LESLTSRIQD QKPPKMPTGF 310 320 330 340 350 360 IKLRTQLFGP KLEKSTMTSE AYKEAVVEAK EHILAGDIFQ IVLSQRFERR TFADPFEIYR 370 380 390 400 410 420 ALRIVNPSPY MAYLQVRGCI LVASSPEILL RSKNRKITNR PLAGTVRRGK TPKEDLMLEK 430 440 450 460 470 480 ELLSDEKQCA EHIMLVDLGR NDVGKVSKPG SVEVKKLKDI EWFSHVMHIS STVVGELLDH 490 500 510 520 530 540 LTSWDALRAV LPVGTVSGAP KVKAMELIDE LEVTRRGPYS GGFGGISFNG DMDIALALRT 550 560 570 580 590 600 MVFPTNTRYD TLYSYKHPQR RREWIAHIQA GAGIVADSNP DDEHRECENK AAALARAIDL 610 620 AESSFLEAPE FTTITPHINN I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway." Niyogi K.K., Fink G.R. Plant Cell 4:721-733(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92354 Genomic DNA. Translation: AAA32739.1. AC005496 Genomic DNA. Translation: AAC35228.1. CP002685 Genomic DNA. Translation: AEC08294.1. AK221602 mRNA. Translation: BAD95154.1.
IPI	IPI00520649.
PIR	JQ1685. S27752.
RefSeq	NP_180530.1. NM_128523.4.
UniGene	At.28056.
3D structure databases
ProteinModelPortal	P32069.
SMR	P32069. Positions 90-604.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G29690.1-P.
Proteomic databases
PaxDb	P32069.
PRIDE	P32069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29690.1; AT2G29690.1; AT2G29690.
GeneID	817519.
KEGG	ath:AT2G29690.
Organism-specific databases
TAIR	At2g29690.
Phylogenomic databases
eggNOG	COG0147.
HOGENOM	HOG000025142.
InParanoid	P32069.
KO	K01657.
OMA	LAFVETF.
PhylomeDB	P32069.
ProtClustDB	PLN02445.
Enzyme and pathway databases
UniPathway	UPA00035; UER00040.
Gene expression databases
Genevestigator	P32069.
GermOnline	AT2G29690. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.60.120.10. 1 hit.
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR005256. Anth_synth_I_PabB. IPR015890. Chorismate-bd_C. [Graphical view]
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR00564. trpE_most. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPX_ARATH

Accession

Primary (citable) accession number: P32069
Secondary accession number(s): Q56XS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program