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Protein

Anthranilate synthase alpha subunit 2, chloroplastic

Gene

ASA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS to produce anthranilate (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Enzyme regulationi

Feedback inhibition by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (AXX17_At5g53930), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (AXX17_At5g28490), Tryptophan synthase (AXX17_At4g31220), Tryptophan synthase, Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 2, chloroplastic (TSB2), Tryptophan synthase (AXX17_At4g31220)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G29690-MONOMER
UniPathwayiUPA00035; UER00040

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase alpha subunit 2, chloroplastic (EC:4.1.3.27)
Alternative name(s):
Anthranilate synthase component 1-2
Anthranilate synthase component I-2
Gene namesi
Name:ASA2
Ordered Locus Names:At2g29690
ORF Names:T27A16.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G29690
TAIRilocus:2005520 AT2G29690

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 87ChloroplastSequence analysisAdd BLAST87
ChainiPRO_000003579088 – 621Anthranilate synthase alpha subunit 2, chloroplasticAdd BLAST534

Proteomic databases

PaxDbiP32069
PRIDEiP32069

Expressioni

Gene expression databases

ExpressionAtlasiP32069 baseline and differential
GenevisibleiP32069 AT

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit and a large alpha subunit.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G29690.1

Structurei

3D structure databases

ProteinModelPortaliP32069
SMRiP32069
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1223 Eukaryota
COG0147 LUCA
HOGENOMiHOG000025142
InParanoidiP32069
KOiK01657
OMAiIAGTFKR
OrthoDBiEOG0936065Q
PhylomeDBiP32069

Family and domain databases

Gene3Di3.60.120.10, 1 hit
InterProiView protein in InterPro
IPR005801 ADC_synthase
IPR019999 Anth_synth_I-like
IPR006805 Anth_synth_I_N
IPR005256 Anth_synth_I_PabB
IPR015890 Chorismate_C
PfamiView protein in Pfam
PF04715 Anth_synt_I_N, 1 hit
PF00425 Chorismate_bind, 1 hit
PRINTSiPR00095 ANTSNTHASEI
SUPFAMiSSF56322 SSF56322, 1 hit
TIGRFAMsiTIGR00564 trpE_most, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVSISAVK SDFFTVEAIA VTHHRTPHPP HFPSLRFPLS LKSPPATSLN
60 70 80 90 100
LVAGSKLLHF SRRLPSIKCS YTPSLDLSEE QFTKFKKASE KGNLVPLFRC
110 120 130 140 150
VFSDHLTPIL AYRCLVKEDD RDAPSFLFES VEPGSQSSNI GRYSVVGAQP
160 170 180 190 200
TIEIVAKGNV VTVMDHGASL RTEEEVDDPM MVPQKIMEEW NPQGIDELPE
210 220 230 240 250
AFCGGWVGYF SYDTVRYVEK KKLPFSNAPE DDRSLPDVNL GLYDDVIVFD
260 270 280 290 300
HVEKKAYVIH WVRIDKDRSV EENFREGMNR LESLTSRIQD QKPPKMPTGF
310 320 330 340 350
IKLRTQLFGP KLEKSTMTSE AYKEAVVEAK EHILAGDIFQ IVLSQRFERR
360 370 380 390 400
TFADPFEIYR ALRIVNPSPY MAYLQVRGCI LVASSPEILL RSKNRKITNR
410 420 430 440 450
PLAGTVRRGK TPKEDLMLEK ELLSDEKQCA EHIMLVDLGR NDVGKVSKPG
460 470 480 490 500
SVEVKKLKDI EWFSHVMHIS STVVGELLDH LTSWDALRAV LPVGTVSGAP
510 520 530 540 550
KVKAMELIDE LEVTRRGPYS GGFGGISFNG DMDIALALRT MVFPTNTRYD
560 570 580 590 600
TLYSYKHPQR RREWIAHIQA GAGIVADSNP DDEHRECENK AAALARAIDL
610 620
AESSFLEAPE FTTITPHINN I
Length:621
Mass (Da):69,816
Last modified:October 1, 1993 - v1
Checksum:iD4039FE58A420967
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85F → L in BAD95154 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92354 Genomic DNA Translation: AAA32739.1
AC005496 Genomic DNA Translation: AAC35228.1
CP002685 Genomic DNA Translation: AEC08294.1
AK221602 mRNA Translation: BAD95154.1
PIRiJQ1685
S27752
RefSeqiNP_180530.1, NM_128523.5
UniGeneiAt.28056

Genome annotation databases

EnsemblPlantsiAT2G29690.1; AT2G29690.1; AT2G29690
GeneIDi817519
GrameneiAT2G29690.1; AT2G29690.1; AT2G29690
KEGGiath:AT2G29690

Similar proteinsi

Entry informationi

Entry nameiTRPX_ARATH
AccessioniPrimary (citable) accession number: P32069
Secondary accession number(s): Q56XS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 25, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health