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Protein

Anthranilate synthase alpha subunit 2, chloroplastic

Gene

ASA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS to produce anthranilate (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Enzyme regulationi

Feedback inhibition by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase beta chain 2, chloroplastic (TSB2)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G29690-MONOMER.
UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase alpha subunit 2, chloroplastic (EC:4.1.3.27)
Alternative name(s):
Anthranilate synthase component 1-2
Anthranilate synthase component I-2
Gene namesi
Name:ASA2
Ordered Locus Names:At2g29690
ORF Names:T27A16.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29690.

Subcellular locationi

GO - Cellular componenti

  • anthranilate synthase complex Source: TAIR
  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8787ChloroplastSequence analysisAdd
BLAST
Chaini88 – 621534Anthranilate synthase alpha subunit 2, chloroplasticPRO_0000035790Add
BLAST

Proteomic databases

PaxDbiP32069.
PRIDEiP32069.

Expressioni

Gene expression databases

GenevisibleiP32069. AT.

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit and a large alpha subunit.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G29690.1.

Structurei

3D structure databases

ProteinModelPortaliP32069.
SMRiP32069. Positions 83-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1223. Eukaryota.
COG0147. LUCA.
HOGENOMiHOG000025142.
InParanoidiP32069.
KOiK01657.
OMAiEHRECEN.
OrthoDBiEOG0936065Q.
PhylomeDBiP32069.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVSISAVK SDFFTVEAIA VTHHRTPHPP HFPSLRFPLS LKSPPATSLN
60 70 80 90 100
LVAGSKLLHF SRRLPSIKCS YTPSLDLSEE QFTKFKKASE KGNLVPLFRC
110 120 130 140 150
VFSDHLTPIL AYRCLVKEDD RDAPSFLFES VEPGSQSSNI GRYSVVGAQP
160 170 180 190 200
TIEIVAKGNV VTVMDHGASL RTEEEVDDPM MVPQKIMEEW NPQGIDELPE
210 220 230 240 250
AFCGGWVGYF SYDTVRYVEK KKLPFSNAPE DDRSLPDVNL GLYDDVIVFD
260 270 280 290 300
HVEKKAYVIH WVRIDKDRSV EENFREGMNR LESLTSRIQD QKPPKMPTGF
310 320 330 340 350
IKLRTQLFGP KLEKSTMTSE AYKEAVVEAK EHILAGDIFQ IVLSQRFERR
360 370 380 390 400
TFADPFEIYR ALRIVNPSPY MAYLQVRGCI LVASSPEILL RSKNRKITNR
410 420 430 440 450
PLAGTVRRGK TPKEDLMLEK ELLSDEKQCA EHIMLVDLGR NDVGKVSKPG
460 470 480 490 500
SVEVKKLKDI EWFSHVMHIS STVVGELLDH LTSWDALRAV LPVGTVSGAP
510 520 530 540 550
KVKAMELIDE LEVTRRGPYS GGFGGISFNG DMDIALALRT MVFPTNTRYD
560 570 580 590 600
TLYSYKHPQR RREWIAHIQA GAGIVADSNP DDEHRECENK AAALARAIDL
610 620
AESSFLEAPE FTTITPHINN I
Length:621
Mass (Da):69,816
Last modified:October 1, 1993 - v1
Checksum:iD4039FE58A420967
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851F → L in BAD95154 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92354 Genomic DNA. Translation: AAA32739.1.
AC005496 Genomic DNA. Translation: AAC35228.1.
CP002685 Genomic DNA. Translation: AEC08294.1.
AK221602 mRNA. Translation: BAD95154.1.
PIRiJQ1685.
S27752.
RefSeqiNP_180530.1. NM_128523.4.
UniGeneiAt.28056.

Genome annotation databases

EnsemblPlantsiAT2G29690.1; AT2G29690.1; AT2G29690.
GeneIDi817519.
GrameneiAT2G29690.1; AT2G29690.1; AT2G29690.
KEGGiath:AT2G29690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92354 Genomic DNA. Translation: AAA32739.1.
AC005496 Genomic DNA. Translation: AAC35228.1.
CP002685 Genomic DNA. Translation: AEC08294.1.
AK221602 mRNA. Translation: BAD95154.1.
PIRiJQ1685.
S27752.
RefSeqiNP_180530.1. NM_128523.4.
UniGeneiAt.28056.

3D structure databases

ProteinModelPortaliP32069.
SMRiP32069. Positions 83-607.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G29690.1.

Proteomic databases

PaxDbiP32069.
PRIDEiP32069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29690.1; AT2G29690.1; AT2G29690.
GeneIDi817519.
GrameneiAT2G29690.1; AT2G29690.1; AT2G29690.
KEGGiath:AT2G29690.

Organism-specific databases

TAIRiAT2G29690.

Phylogenomic databases

eggNOGiKOG1223. Eukaryota.
COG0147. LUCA.
HOGENOMiHOG000025142.
InParanoidiP32069.
KOiK01657.
OMAiEHRECEN.
OrthoDBiEOG0936065Q.
PhylomeDBiP32069.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BioCyciARA:AT2G29690-MONOMER.

Miscellaneous databases

PROiP32069.

Gene expression databases

GenevisibleiP32069. AT.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPX_ARATH
AccessioniPrimary (citable) accession number: P32069
Secondary accession number(s): Q56XS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.