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P32068 (TRPE_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Anthranilate synthase component I-1, chloroplastic

EC=4.1.3.27
Gene names
Name:ASA1
Ordered Locus Names:At5g05730
ORF Names:MJJ3.14
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.

Subunit structure

Tetramer of two components I and two components II.

Subcellular location

Plastidchloroplast Probable.

Miscellaneous

Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.

Sequence similarities

Belongs to the anthranilate synthase component I family.

Sequence caution

The sequence AY072013 differs from that shown. Reason: Frameshift at position 197.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P32068-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6060Chloroplast Potential
Chain61 – 595535Anthranilate synthase component I-1, chloroplastic
PRO_0000035789

Experimental info

Sequence conflict591P → R in AY072013. Ref.4
Sequence conflict121 – 1233QMS → LLA in AY072013. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 674175416A2726F0

FASTA59566,312
        10         20         30         40         50         60 
MSSSMNVATM QALTFSRRLL PSVASRYLSS SSVTVTGYSG RSSAYAPSFR SIKCVSVSPE 

        70         80         90        100        110        120 
ASIVSDTKKL ADASKSTNLI PIYRCIFSDQ LTPVLAYRCL VKEDDREAPS FLFESVEPGS 

       130        140        150        160        170        180 
QMSSVGRYSV VGAQPAMEIV AKENKVIVMD HNNETMTEEF VEDPMEIPRK ISEKWNPDPQ 

       190        200        210        220        230        240 
LVQDLPDAFC GGWVGFFSYD TVRYVEKRKL PFSKAPEDDR NLPDMHLGLY DDVVVFDHVE 

       250        260        270        280        290        300 
KKAYVIHWIR LDGSLPYEKA YSNGMQHLEN LVAKLHDIEP PKLAAGNVNL QTRQFGPSLD 

       310        320        330        340        350        360 
NSNVTCEEYK EAVVKAKEHI LAGDIFQIVL SQRFERRTFA DPFEVYRALR VVNPSPYMGY 

       370        380        390        400        410        420 
LQARGCILVA SSPEILTKVK QNKIVNRPLA GTSKRGKNEV EDKRLEKELL ENEKQCAEHI 

       430        440        450        460        470        480 
MLVDLGRNDV GKVTKYGSVK VEKLMNIERY SHVMHISSTV TGELQDGLTC WDVLRAALPV 

       490        500        510        520        530        540 
GTVSGAPKVK AMELIDELEP TRRGPYSGGF GGVSFTGDMD IALSLRTIVF PTACQYNTMY 

       550        560        570        580        590 
SYKDANKRRE WVAYLQAGAG VVADSDPQDE HCECQNKAAG LARAIDLAES AFVKK 

« Hide

References

« Hide 'large scale' references
[1]"Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway."
Niyogi K.K., Fink G.R.
Plant Cell 4:721-733(1992) [PubMed: 1392592] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92353 Genomic DNA. Translation: AAA32738.1.
AB005237 Genomic DNA. Translation: BAB09667.1.
CP002688 Genomic DNA. Translation: AED90915.1.
AY072013 mRNA. No translation available.
IPIIPI00545909.
PIRJQ1684.
RefSeqNP_196192.1. NM_120655.3.
UniGeneAt.285.

3D structure databases

ProteinModelPortalP32068.
SMRP32068. Positions 83-587.
ModBaseSearch...

Protein-protein interaction databases

STRINGP32068.

Proteomic databases

PRIDEP32068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G05730.1; AT5G05730.1; AT5G05730.
GeneID830457.
GenomeReviewsGene locus AT5G05730 in contig BA000015_GR.
KEGGath:AT5G05730.
NMPDRfig|3702.1.peg.22686.

Organism-specific databases

TAIRAt5g05730.

Phylogenomic databases

eggNOGKOG1223.
GeneTreeEPGT00050000007781.
HOGENOMHBG507440.
InParanoidP32068.
OMARTIYGRA.
PhylomeDBP32068.
ProtClustDBPLN02445.

Gene expression databases

ArrayExpressP32068.
GenevestigatorP32068.
GermOnlineAT5G05730. Arabidopsis thaliana.

Family and domain databases

InterProIPR005801. ADC_synthase.
IPR019999. Anth_synth_I.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate-bd_C.
[Graphical view]
Gene3DG3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
KOK01657.
PfamPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSPR00095. ANTSNTHASEI.
SUPFAMSSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMsTIGR00564. TrpE_most. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRPE_ARATH
AccessionPrimary (citable) accession number: P32068
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families