Reviewed,
UniProtKB/Swiss-Prot P32068 (TRPE_ARATH)
Last modified
November 3, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Anthranilate synthase component I-1, chloroplastic EC=4.1.3.27 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 595 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate. |
| Pathway | Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5. |
| Subunit structure | Tetramer of two components I and two components II. |
| Subcellular location | Plastid › chloroplast Probable. |
| Miscellaneous | Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity. |
| Sequence similarities | Belongs to the anthranilate synthase component I family. |
| Sequence caution | The sequence AY072013 differs from that shown. Reason: Frameshift at position 197. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Molecular function | Lyase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | auxin biosynthetic process Inferred from genetic interaction. Source: TAIR lateral root primordium developmentInferred from mutant phenotype. Source: TAIR response to ethylene stimulusInferred from genetic interaction. Source: TAIR tryptophan biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | anthranilate synthase complex Ref.1 Non-traceable author statement. Source: TAIR chloroplastInferred from direct assay. Source: TAIR |
| Molecular function | anthranilate synthase activity Ref.1 Inferred from mutant phenotype. Source: TAIR |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 60 | 60 | Chloroplast Potential | ||||||
| Chain | 61 – 595 | 535 | Anthranilate synthase component I-1, chloroplastic | PRO_0000035789 | |||||
Experimental info | |||||||||
| Sequence conflict | 59 | 1 | P → R in AY072013. Ref.3 | ||||||
| Sequence conflict | 121 – 123 | 3 | QMS → LLA in AY072013. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway." Niyogi K.K., Fink G.R. Plant Cell 4:721-733(1992) [PubMed: 1392592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M92353 Genomic DNA. Translation: AAA32738.1. AB005237 Genomic DNA. Translation: BAB09667.1. AY072013 mRNA. No translation available. | |
| IPI | IPI00545909. |
| PIR | JQ1684. |
| RefSeq | NP_196192.1. |
| UniGene | At.285 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1K0G based on UniProtKB P05041. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P32068. |
Proteomic databases | |
| PRIDE | P32068. |
Genome annotation databases | |
| GeneID | 830457. |
| GenomeReviews | Gene locus AT5G05730 in contig BA000015_GR. |
| KEGG | ath:AT5G05730. |
| NMPDR | fig|3702.1.peg.22686. |
Organism-specific databases | |
| TAIR | At5g05730. |
Phylogenomic databases | |
| OMA | MELNIAI. |
Enzyme and pathway databases | |
| BRENDA | 4.1.3.27. 302. |
Gene expression databases | |
| ArrayExpress | P32068. |
| Genevestigator | P32068. |
| GermOnline | AT5G05730. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR005256. Anth_synthI. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. [Graphical view] |
| Gene3D | G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit. |
| PANTHER | PTHR11236. TRPE_1_chor_bd. 1 hit. |
| Pfam | PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view] |
| PRINTS | PR00095. ANTSNTHASEI. |
| ProDom | PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR00564. trpE_most. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | TRPE_ARATH | ||||||||
| Accession | Primary (citable) accession number: P32068 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


