P32068 (TRPE_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component I-1, chloroplastic
EC=4.1.3.27

Gene names

Name:	ASA1
Ordered Locus Names:	At5g05730
ORF Names:	MJJ3.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Subcellular location	Plastid › chloroplast Probable.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.
Sequence caution	The sequence AY072013 differs from that shown. Reason: Frameshift at position 197.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin biosynthetic process Inferred from genetic interaction. Source: TAIR lateral root primordium development Inferred from mutant phenotype. Source: TAIR response to ethylene stimulus Inferred from genetic interaction. Source: TAIR response to wounding Inferred from expression pattern Ref.1. Source: TAIR tryptophan biosynthetic process Traceable author statement Ref.1. Source: TAIR
Cellular component	anthranilate synthase complex Non-traceable author statement Ref.1. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	anthranilate synthase activity Inferred from mutant phenotype Ref.1. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P32068-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 60	60	Chloroplast Potential
	Chain	61 – 595	535	Anthranilate synthase component I-1, chloroplastic		PRO_0000035789
Experimental info
	Sequence conflict	59	1	P → R in AY072013. Ref.4
	Sequence conflict	121 – 123	3	QMS → LLA in AY072013. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 674175416A2726F0	FASTA	595	66,312
	10 20 30 40 50 60 MSSSMNVATM QALTFSRRLL PSVASRYLSS SSVTVTGYSG RSSAYAPSFR SIKCVSVSPE 70 80 90 100 110 120 ASIVSDTKKL ADASKSTNLI PIYRCIFSDQ LTPVLAYRCL VKEDDREAPS FLFESVEPGS 130 140 150 160 170 180 QMSSVGRYSV VGAQPAMEIV AKENKVIVMD HNNETMTEEF VEDPMEIPRK ISEKWNPDPQ 190 200 210 220 230 240 LVQDLPDAFC GGWVGFFSYD TVRYVEKRKL PFSKAPEDDR NLPDMHLGLY DDVVVFDHVE 250 260 270 280 290 300 KKAYVIHWIR LDGSLPYEKA YSNGMQHLEN LVAKLHDIEP PKLAAGNVNL QTRQFGPSLD 310 320 330 340 350 360 NSNVTCEEYK EAVVKAKEHI LAGDIFQIVL SQRFERRTFA DPFEVYRALR VVNPSPYMGY 370 380 390 400 410 420 LQARGCILVA SSPEILTKVK QNKIVNRPLA GTSKRGKNEV EDKRLEKELL ENEKQCAEHI 430 440 450 460 470 480 MLVDLGRNDV GKVTKYGSVK VEKLMNIERY SHVMHISSTV TGELQDGLTC WDVLRAALPV 490 500 510 520 530 540 GTVSGAPKVK AMELIDELEP TRRGPYSGGF GGVSFTGDMD IALSLRTIVF PTACQYNTMY 550 560 570 580 590 SYKDANKRRE WVAYLQAGAG VVADSDPQDE HCECQNKAAG LARAIDLAES AFVKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway." Niyogi K.K., Fink G.R. Plant Cell 4:721-733(1992) [PubMed: 1392592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92353 Genomic DNA. Translation: AAA32738.1. AB005237 Genomic DNA. Translation: BAB09667.1. CP002688 Genomic DNA. Translation: AED90915.1. AY072013 mRNA. No translation available.
IPI	IPI00545909.
PIR	JQ1684.
RefSeq	NP_196192.1. NM_120655.3.
UniGene	At.285.
3D structure databases
ProteinModelPortal	P32068.
SMR	P32068. Positions 83-587.
ModBase	Search...
Protein-protein interaction databases
STRING	P32068.
Proteomic databases
PRIDE	P32068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G05730.1; AT5G05730.1; AT5G05730.
GeneID	830457.
GenomeReviews	Gene locus AT5G05730 in contig BA000015_GR.
KEGG	ath:AT5G05730.
NMPDR	fig|3702.1.peg.22686.
Organism-specific databases
TAIR	At5g05730.
Phylogenomic databases
eggNOG	KOG1223.
GeneTree	EPGT00050000007781.
HOGENOM	HBG507440.
InParanoid	P32068.
OMA	RTIYGRA.
PhylomeDB	P32068.
ProtClustDB	PLN02445.
Gene expression databases
ArrayExpress	P32068.
Genevestigator	P32068.
GermOnline	AT5G05730. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR005256. Anth_synth_I_PabB. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
KO	K01657.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR00564. TrpE_most. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_ARATH

Accession

Primary (citable) accession number: P32068

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program