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Protein

Prophage integrase IntA

Gene

intA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Part of the cryptic P4-like prophage CP4-57, it excises the prophage when overexpressed, which also requires integration host factor (encoded by ihfA and ihfB) (PubMed:7511583). Overexpression of AlpA leads to excision of the CP4-57 prophage, which inactivates ssrA (the gene upstream of the prophage) that encodes tmRNA which is required to rescue stalled ribosomes in a process known as trans-translation (PubMed:7511582).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei373O-(3'-phospho-DNA)-tyrosine intermediateBy similarity1

GO - Molecular functioni

  • DNA binding Source: InterPro
  • prophage integrase activity Source: EcoCyc

GO - Biological processi

  • provirus excision Source: EcoCyc
  • viral entry into host cell Source: UniProtKB-KW
  • viral genome integration into host DNA Source: UniProtKB-KW

Keywordsi

Biological processDNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Enzyme and pathway databases

BioCyciEcoCyc:EG11783-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Prophage integrase IntACurated
Alternative name(s):
Prophage CP4-57 integrase SlpA1 Publication
Gene namesi
Name:intA
Synonyms:intX, slpA1 Publication
Ordered Locus Names:b2622, JW2602
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11783 intA

Subcellular locationi

GO - Cellular componenti

  • provirus Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Loss of excision of its prophage (PubMed:7511583).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975111 – 413Prophage integrase IntAAdd BLAST413

Proteomic databases

PaxDbiP32053
PRIDEiP32053

Expressioni

Inductioni

By AlpA (PubMed:7511582).1 Publication

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260622, 13 interactors
DIPiDIP-10034N
IntActiP32053, 26 interactors
STRINGi316407.85675485

Structurei

3D structure databases

ProteinModelPortaliP32053
SMRiP32053
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFP Bacteria
COG0582 LUCA
HOGENOMiHOG000263105
InParanoidiP32053
OMAiYIERRRP
PhylomeDBiP32053

Family and domain databases

Gene3Di1.10.150.130, 1 hit
1.10.443.10, 1 hit
3.30.160.390, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013762 Integrase-like_cat_sf
IPR002104 Integrase_catalytic
IPR038488 Integrase_DNA-bd_sf
IPR025166 Integrase_DNA_bind_dom
IPR010998 Integrase_recombinase_N
PfamiView protein in Pfam
PF13356 Arm-DNA-bind_3, 1 hit
PF00589 Phage_integrase, 1 hit
SUPFAMiSSF56349 SSF56349, 1 hit

Sequencei

Sequence statusi: Complete.

P32053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKTKPLTD TEIKAAKPKD ADYQLYDGDG LTLLIKSSGS KLWQFRYYRP
60 70 80 90 100
LTKQRTKQSF GAYPAVSLSD ARKLRAESKV LLAKDIDPQE HQKEQVRNSQ
110 120 130 140 150
EAKTNTFLLV AERWWNVKKT SVTEDYADDI WRSLERDIFP AIGDISITEI
160 170 180 190 200
KAHTLVKAVQ PVQARGALET VRRLCQRINE VMIYAQNTGL IDAVPSVNIG
210 220 230 240 250
KAFEKPQKKN MPSIRPDQLP QLMHTMRTAS ISMSTRCLFM WQLLTITRPA
260 270 280 290 300
EAAEARWDEI DFNASEWKIP AARMKMNRDH TVPLSDGALA ILEMMKPLSG
310 320 330 340 350
GREFIFPSRI KPNQPMNSQT VNAALKRAGL GGVLVSHGLR SIASTALNEE
360 370 380 390 400
GFPPDVIEAA LAHVDKNEVR RAYNRSDYLE QRRPMMQWWA DLVKAADSGS
410
IVLTHLSKIR LVG
Length:413
Mass (Da):46,652
Last modified:February 1, 1994 - v2
Checksum:iE050A1F60860BCB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03737 Unassigned DNA Translation: AAA18417.1
U36840 Genomic DNA Translation: AAA79791.1
U00096 Genomic DNA Translation: AAC75670.1
AP009048 Genomic DNA Translation: BAA20921.2
D12501 Genomic DNA Translation: BAA20981.1
PIRiH65040
RefSeqiNP_417111.1, NC_000913.3
WP_000101723.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75670; AAC75670; b2622
BAA20921; BAA20921; BAA20921
GeneIDi946160
KEGGiecj:JW2602
eco:b2622
PATRICifig|1411691.4.peg.4117

Similar proteinsi

Entry informationi

Entry nameiINTA_ECOLI
AccessioniPrimary (citable) accession number: P32053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: June 20, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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