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Protein

Prophage CP4-57 integrase

Gene

intA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei373 – 3731O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  • prophage integrase activity Source: EcoCyc
  • sequence-specific DNA binding Source: GO_Central

GO - Biological processi

  • DNA recombination Source: UniProtKB-KW
  • provirus excision Source: EcoCyc
  • viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Enzyme and pathway databases

BioCyciEcoCyc:EG11783-MONOMER.
ECOL316407:JW2602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prophage CP4-57 integrase
Gene namesi
Name:intA
Synonyms:intX, slpA
Ordered Locus Names:b2622, JW2602
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11783. intA.

Subcellular locationi

GO - Cellular componenti

  • provirus Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Prophage CP4-57 integrasePRO_0000197511Add
BLAST

Proteomic databases

PaxDbiP32053.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069592EBI-552967,EBI-542707

Protein-protein interaction databases

BioGridi4260622. 13 interactions.
DIPiDIP-10034N.
IntActiP32053. 26 interactions.
MINTiMINT-1219095.
STRINGi511145.b2622.

Structurei

3D structure databases

ProteinModelPortaliP32053.
SMRiP32053. Positions 8-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFP. Bacteria.
COG0582. LUCA.
HOGENOMiHOG000263105.
InParanoidiP32053.
OMAiDHAKDIW.
OrthoDBiEOG6X3W2Z.
PhylomeDBiP32053.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR025166. DUF4102.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR023109. Integrase_recombinase_N.
[Graphical view]
PfamiPF13356. DUF4102. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P32053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKTKPLTD TEIKAAKPKD ADYQLYDGDG LTLLIKSSGS KLWQFRYYRP
60 70 80 90 100
LTKQRTKQSF GAYPAVSLSD ARKLRAESKV LLAKDIDPQE HQKEQVRNSQ
110 120 130 140 150
EAKTNTFLLV AERWWNVKKT SVTEDYADDI WRSLERDIFP AIGDISITEI
160 170 180 190 200
KAHTLVKAVQ PVQARGALET VRRLCQRINE VMIYAQNTGL IDAVPSVNIG
210 220 230 240 250
KAFEKPQKKN MPSIRPDQLP QLMHTMRTAS ISMSTRCLFM WQLLTITRPA
260 270 280 290 300
EAAEARWDEI DFNASEWKIP AARMKMNRDH TVPLSDGALA ILEMMKPLSG
310 320 330 340 350
GREFIFPSRI KPNQPMNSQT VNAALKRAGL GGVLVSHGLR SIASTALNEE
360 370 380 390 400
GFPPDVIEAA LAHVDKNEVR RAYNRSDYLE QRRPMMQWWA DLVKAADSGS
410
IVLTHLSKIR LVG
Length:413
Mass (Da):46,652
Last modified:February 1, 1994 - v2
Checksum:iE050A1F60860BCB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03737 Unassigned DNA. Translation: AAA18417.1.
U36840 Genomic DNA. Translation: AAA79791.1.
U00096 Genomic DNA. Translation: AAC75670.1.
AP009048 Genomic DNA. Translation: BAA20921.2.
D12501 Genomic DNA. Translation: BAA20981.1.
PIRiH65040.
RefSeqiNP_417111.1. NC_000913.3.
WP_000101723.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75670; AAC75670; b2622.
BAA20921; BAA20921; BAA20921.
GeneIDi946160.
KEGGiecj:JW2602.
eco:b2622.
PATRICi32120641. VBIEscCol129921_2719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03737 Unassigned DNA. Translation: AAA18417.1.
U36840 Genomic DNA. Translation: AAA79791.1.
U00096 Genomic DNA. Translation: AAC75670.1.
AP009048 Genomic DNA. Translation: BAA20921.2.
D12501 Genomic DNA. Translation: BAA20981.1.
PIRiH65040.
RefSeqiNP_417111.1. NC_000913.3.
WP_000101723.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32053.
SMRiP32053. Positions 8-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260622. 13 interactions.
DIPiDIP-10034N.
IntActiP32053. 26 interactions.
MINTiMINT-1219095.
STRINGi511145.b2622.

Proteomic databases

PaxDbiP32053.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75670; AAC75670; b2622.
BAA20921; BAA20921; BAA20921.
GeneIDi946160.
KEGGiecj:JW2602.
eco:b2622.
PATRICi32120641. VBIEscCol129921_2719.

Organism-specific databases

EchoBASEiEB1731.
EcoGeneiEG11783. intA.

Phylogenomic databases

eggNOGiENOG4105DFP. Bacteria.
COG0582. LUCA.
HOGENOMiHOG000263105.
InParanoidiP32053.
OMAiDHAKDIW.
OrthoDBiEOG6X3W2Z.
PhylomeDBiP32053.

Enzyme and pathway databases

BioCyciEcoCyc:EG11783-MONOMER.
ECOL316407:JW2602-MONOMER.

Miscellaneous databases

PROiP32053.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR025166. DUF4102.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR023109. Integrase_recombinase_N.
[Graphical view]
PfamiPF13356. DUF4102. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Excision of a P4-like cryptic prophage leads to Alp protease expression in Escherichia coli."
    Kirby J.E., Trempy J.E., Gottesman S.
    J. Bacteriol. 176:2068-2081(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-99.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli."
    Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.
    Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.
    Strain: K12.

Entry informationi

Entry nameiINTA_ECOLI
AccessioniPrimary (citable) accession number: P32053
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: January 20, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.